LOCUS       BBa_K1159210             928 bp    DNA     linear       22-Dec-2024
DEFINITION  Secretory nLuc_SpyCatcher fusion (IgKappa-SigP_nLuc_SpyCatcher) in 
            RFC[25] N-Part
ACCESSION   BBa_K1159210
VERSION     BBa_K1159210.1  
FEATURES             Location/Qualifiers
     RBS             1..4
                     /label=Consensus Sequence for P. patens
     CDS             5..67
                     /label=Ig Kappa Signal Peptide
     misc_feature    1..67
                     /label=BBa_K1159304
     misc_feature    68..73
                     /label=25/25 scar
     misc_feature    68..73
                     /label=BBa_K1159993
     CDS             74..583
                     /label=NanoLuc Luciferase
     CDS             74..583
                     /label=BBa_K1159001
     CDS             1..583
                     /label=BBa_K1159006
     misc_feature    584..589
                     /label=25/25 scar
     misc_feature    584..589
                     /label=BBa_K1159993
     CDS             590..928
                     /label=N-terminal FbaB (modified SpyCatcher)
     misc_feature    689..691
                     /label=Iso --> Glu
     misc_feature    794..796
                     /label=Met --> Tyr
     misc_feature    680..682
                     /label=Reactive Lys for isopeptide forming reaction
     CDS             590..928
                     /label=BBa_K1159200
ORIGIN
        1 aacaatggaa accgatactc tcctcctctg ggttctcttg ctttgggttc caggatctac
       61 cggcgatacc ggcgtgttca ccctcgagga tttcgttgga gattggagac agaccgctgg
      121 atacaacctt gatcaggttt tggagcaggg tggtgtgtct agtcttttcc agaacctcgg
      181 agtgtctgtg acccctatcc agagaatcgt tctctctggt gagaacggac tcaagatcga
      241 tatccacgtg atcatccctt acgagggact ctcaggtgat cagatgggac agatcgagaa
      301 gattttcaag gtggtgtacc ctgttgatga tcaccacttc aaggtgatcc tccactacgg
      361 aactctcgtg atcgatggtg tgaccccaaa catgatcgat tacttcggaa ggccatacga
      421 gggaatcgct gtgttcgatg gaaagaagat taccgttacc ggaaccctct ggaacggaaa
      481 caagattatc gatgagaggc tcatcaaccc tgatggatct ctcttgttca gggtgaccat
      541 caacggtgtg actggttgga gactttgcga gagaatcctt gctaccggcg ttgatacctt
      601 atcaggttta tcaagtgagc aaggtcagtc cggtgatatg acaattgaag aagatagtgc
      661 tacccatatt aaattctcaa aacgtgatga ggacggcaaa gagttagctg gtgcaactat
      721 ggagttgcgt gattcatctg gtaaaactat tagtacatgg atttcagatg gacaagtgaa
      781 agatttctac ctgtatccag gaaaatatac atttgtcgaa accgcagcac cagacggtta
      841 tgaggtagca actgctatta cctttacagt taatgagcaa ggtcaggtta ctgtaaatgg
      901 caaagcaact aaaggtgacg ctcatatt
//