BBa_K1159200
1
BBa_K1159200
Splitted and engineered N-terminal FbaB for isopeptide bound formation (SpyCatcher) in RFC[25]
2013-09-13T11:00:00Z
2015-05-08T01:09:31Z
''Streptococcus pyogenes''
This part codes for a protein that recognizes and forms a covalent isopeptide bound to a oligopeptide. That oligopeptide can be found under BBa_K1159201. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
false
false
_1471_
0
11507
9
In stock
false
x
false
Dong-Jiunn Jeffery TRUONG
annotation2343366
1
Reactive Lys for isopeptide forming reaction
range2343366
1
91
93
annotation2343345
1
N-terminal FbaB (modified SpyCatcher)
range2343345
1
1
339
annotation2343349
1
Met --> Tyr
range2343349
1
205
207
annotation2343348
1
Iso --> Glu
range2343348
1
100
102
BBa_K1159304
1
BBa_K1159304
Signal Peptide of Ig Kappa chain from <i>Mus musculus</i> in RFC[25] N-part
2013-09-13T11:00:00Z
2015-06-11T01:54:39Z
''Mus musculus''
This part codes for the signal peptide of the Ig Kappa chain from ''Mus musculus''. The signal peptide induces translocation of the protein into the Endoplasmatic Reticulum (ER) for secretion or membrane integration. Protein fused to the C-terminus of this part should be secreted or otherwise if the part fused region contains a transmembrane domain it will be integrated in cytoplasmatic membrane. This part starts with a consensus sequence for Physcomitrella patens which allows efficient translation start. Furthermore, this part is flanked by RFC[25] N-part pre- and suffix for fusions to the C-terminus of this part.
false
false
_1471_
4206
11507
9
In stock
false
x
false
TU Munich 2013
annotation2341499
1
Ig Kappa Signal Peptide
range2341499
1
5
67
annotation2341498
1
Consensus Sequence for P. patens
range2341498
1
1
4
BBa_K1159993
1
BBa_K1159993
25/25 Assembly Scar
2013-09-14T11:00:00Z
2015-05-08T01:09:31Z
Synthetic
Assembly scar between RFC[25] Biobrick and RFC[25] Biobrick.
false
false
_1471_
0
11507
9
Not in stock
false
x
false
TU Munich 2013
annotation2342863
1
25/25 scar
range2342863
1
1
6
BBa_K1159001
1
BBa_K1159001
NanoLuc Luciferase in RFC[25]
2013-05-16T11:00:00Z
2016-01-26T02:12:53Z
x
x
false
false
_1471_
4206
14732
9
In stock
true
x
false
TU Munich 2013
annotation2342942
1
NanoLuc Luciferase
range2342942
1
1
510
BBa_K1159006
1
BBa_K1159006
Secretory NanoLuc Luciferase (IgKappa-SigP_nLuc) in RFC[25] N-Part
2013-09-14T11:00:00Z
2015-05-28T03:52:04Z
Synthetic
NanoLuc Luciferase is engineered ATP-independent luciferase from a deep-sea shrimp which luminescense 2 magnitues higher than these from Renilla reniformis or from Photinus pyralis (firefly). Also the molecular weight of NanoLuc luciferase is twice smaller compared to other luciferase (only 19 kDa). This part encodes for a NanoLuc Luciferase which is fused to a N-terminal signal peptide for secretion (IgKappa signal peptide from Mus musculus) with also works in moss (Physcomitrella patens). This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
false
false
_1471_
4206
11507
9
In stock
false
x
false
TU Munich 2013
component2343006
1
BBa_K1159304
component2343010
1
BBa_K1159001
component2343008
1
BBa_K1159993
annotation2343006
1
BBa_K1159304
range2343006
1
1
67
annotation2343010
1
BBa_K1159001
range2343010
1
74
583
annotation2343008
1
BBa_K1159993
range2343008
1
68
73
BBa_K1159210
1
BBa_K1159210
Secretory nLuc_SpyCatcher fusion (IgKappa-SigP_nLuc_SpyCatcher) in RFC[25] N-Part
2013-09-16T11:00:00Z
2015-05-08T01:09:31Z
Synthetic
Secretory Fusionprotein consisting N-terminal nLuc and C-terminal SpyTag (IgKappa-SigP_nLuc_SpyTag) in RFC[25] N-Part. This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
false
false
_1471_
0
11507
9
In stock
false
x
false
Dong-Jiunn Jeffery TRUONG
component2350296
1
BBa_K1159993
component2350301
1
BBa_K1159200
component2350294
1
BBa_K1159006
annotation2350296
1
BBa_K1159993
range2350296
1
584
589
annotation2350294
1
BBa_K1159006
range2350294
1
1
583
annotation2350301
1
BBa_K1159200
range2350301
1
590
928
BBa_K1159993_sequence
1
accggc
BBa_K1159304_sequence
1
aacaatggaaaccgatactctcctcctctgggttctcttgctttgggttccaggatctaccggcgat
BBa_K1159006_sequence
1
aacaatggaaaccgatactctcctcctctgggttctcttgctttgggttccaggatctaccggcgataccggcgtgttcaccctcgaggatttcgttggagattggagacagaccgctggatacaaccttgatcaggttttggagcagggtggtgtgtctagtcttttccagaacctcggagtgtctgtgacccctatccagagaatcgttctctctggtgagaacggactcaagatcgatatccacgtgatcatcccttacgagggactctcaggtgatcagatgggacagatcgagaagattttcaaggtggtgtaccctgttgatgatcaccacttcaaggtgatcctccactacggaactctcgtgatcgatggtgtgaccccaaacatgatcgattacttcggaaggccatacgagggaatcgctgtgttcgatggaaagaagattaccgttaccggaaccctctggaacggaaacaagattatcgatgagaggctcatcaaccctgatggatctctcttgttcagggtgaccatcaacggtgtgactggttggagactttgcgagagaatccttgct
BBa_K1159200_sequence
1
gttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatatt
BBa_K1159001_sequence
1
gtgttcaccctcgaggatttcgttggagattggagacagaccgctggatacaaccttgatcaggttttggagcagggtggtgtgtctagtcttttccagaacctcggagtgtctgtgacccctatccagagaatcgttctctctggtgagaacggactcaagatcgatatccacgtgatcatcccttacgagggactctcaggtgatcagatgggacagatcgagaagattttcaaggtggtgtaccctgttgatgatcaccacttcaaggtgatcctccactacggaactctcgtgatcgatggtgtgaccccaaacatgatcgattacttcggaaggccatacgagggaatcgctgtgttcgatggaaagaagattaccgttaccggaaccctctggaacggaaacaagattatcgatgagaggctcatcaaccctgatggatctctcttgttcagggtgaccatcaacggtgtgactggttggagactttgcgagagaatccttgct
BBa_K1159210_sequence
1
aacaatggaaaccgatactctcctcctctgggttctcttgctttgggttccaggatctaccggcgataccggcgtgttcaccctcgaggatttcgttggagattggagacagaccgctggatacaaccttgatcaggttttggagcagggtggtgtgtctagtcttttccagaacctcggagtgtctgtgacccctatccagagaatcgttctctctggtgagaacggactcaagatcgatatccacgtgatcatcccttacgagggactctcaggtgatcagatgggacagatcgagaagattttcaaggtggtgtaccctgttgatgatcaccacttcaaggtgatcctccactacggaactctcgtgatcgatggtgtgaccccaaacatgatcgattacttcggaaggccatacgagggaatcgctgtgttcgatggaaagaagattaccgttaccggaaccctctggaacggaaacaagattatcgatgagaggctcatcaaccctgatggatctctcttgttcagggtgaccatcaacggtgtgactggttggagactttgcgagagaatccttgctaccggcgttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatatt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z