LOCUS       BBa_K1159211             928 bp    DNA     linear       22-Nov-2024
DEFINITION  Secretory SpyCatcher_nLuc fusion (IgKappa-SigP_SpyCatcher_nLuc) in 
            RFC[25] N-Part
ACCESSION   BBa_K1159211
VERSION     BBa_K1159211.1  
FEATURES             Location/Qualifiers
     RBS             1..4
                     /label=Consensus Sequence for P. patens
     CDS             5..67
                     /label=Ig Kappa Signal Peptide
     misc_feature    1..67
                     /label=BBa_K1159304
     misc_feature    68..73
                     /label=25/25 scar
     misc_feature    68..73
                     /label=BBa_K1159993
     CDS             74..412
                     /label=N-terminal FbaB (modified SpyCatcher)
     misc_feature    173..175
                     /label=Iso --> Glu
     misc_feature    278..280
                     /label=Met --> Tyr
     misc_feature    164..166
                     /label=Reactive Lys for isopeptide forming reaction
     CDS             74..412
                     /label=BBa_K1159200
     CDS             1..412
                     /label=BBa_K1159202
     misc_feature    413..418
                     /label=25/25 scar
     misc_feature    413..418
                     /label=BBa_K1159993
     CDS             419..928
                     /label=NanoLuc Luciferase
     CDS             419..928
                     /label=BBa_K1159001
ORIGIN
        1 aacaatggaa accgatactc tcctcctctg ggttctcttg ctttgggttc caggatctac
       61 cggcgatacc ggcgttgata ccttatcagg tttatcaagt gagcaaggtc agtccggtga
      121 tatgacaatt gaagaagata gtgctaccca tattaaattc tcaaaacgtg atgaggacgg
      181 caaagagtta gctggtgcaa ctatggagtt gcgtgattca tctggtaaaa ctattagtac
      241 atggatttca gatggacaag tgaaagattt ctacctgtat ccaggaaaat atacatttgt
      301 cgaaaccgca gcaccagacg gttatgaggt agcaactgct attaccttta cagttaatga
      361 gcaaggtcag gttactgtaa atggcaaagc aactaaaggt gacgctcata ttaccggcgt
      421 gttcaccctc gaggatttcg ttggagattg gagacagacc gctggataca accttgatca
      481 ggttttggag cagggtggtg tgtctagtct tttccagaac ctcggagtgt ctgtgacccc
      541 tatccagaga atcgttctct ctggtgagaa cggactcaag atcgatatcc acgtgatcat
      601 cccttacgag ggactctcag gtgatcagat gggacagatc gagaagattt tcaaggtggt
      661 gtaccctgtt gatgatcacc acttcaaggt gatcctccac tacggaactc tcgtgatcga
      721 tggtgtgacc ccaaacatga tcgattactt cggaaggcca tacgagggaa tcgctgtgtt
      781 cgatggaaag aagattaccg ttaccggaac cctctggaac ggaaacaaga ttatcgatga
      841 gaggctcatc aaccctgatg gatctctctt gttcagggtg accatcaacg gtgtgactgg
      901 ttggagactt tgcgagagaa tccttgct
//