LOCUS BBa_K1159212 2725 bp DNA linear 08-Dec-2025 DEFINITION Membrane-anchored SpyCatcher with secretory SpyTag_nLuc reporter fusion translational unit ACCESSION BBa_K1159212 VERSION BBa_K1159212.1 FEATURES Location/Qualifiers RBS 1..4 /label=Consensus Sequence for ''P. patens'' CDS 5..100 /label=SERK Signal Peptide misc_feature 1..100 /label=BBa_K1159303 misc_feature 101..106 /label=25/25 scar misc_feature 101..106 /label=BBa_K1159993 CDS 107..445 /label=N-terminal FbaB (modified SpyCatcher) misc_feature 206..208 /label=Iso --> Glu misc_feature 311..313 /label=Met --> Tyr misc_feature 197..199 /label=Reactive Lys for isopeptide forming reaction CDS 107..445 /label=BBa_K1159200 CDS 1..445 /label=BBa_K1159204 misc_feature 446..451 /label=25/25 scar misc_feature 446..451 /label=BBa_K1159993 misc_feature 458..481 /label=''Strep''-tag II misc_feature 485..505 /label=TEV cleavage site misc_feature 452..508 /label=BBa_K1159309 misc_feature 509..514 /label=25/25 scar misc_feature 509..514 /label=BBa_K1159993 CDS 515..700 /label=Transmembrane Domain of SERK Receptor from ''P. patens'' misc_feature 515..700 /label=BBa_K1159305 misc_feature 701..706 /label=25/25 scar misc_feature 701..706 /label=BBa_K1159993 misc_feature 707..730 /label=MiddleLinker misc_feature 707..730 /label=BBa_K243005 misc_feature 731..736 /label=25/25 scar misc_feature 731..736 /label=BBa_K1159993 CDS 737..1447 /label=GFPmut1 CDS 737..1447 /label=BBa_K1159311 CDS 515..1447 /label=BBa_K1159315 CDS 452..1447 /label=BBa_K1159316 CDS 1..1447 /label=BBa_K1159206 misc_feature 1448..1464 /label=25/10 NC scar misc_feature 1448..1464 /label=BBa_K1159996 RBS 1465..2091 /label=Polioviral IRES RBS 1465..2091 /label=BBa_K1159300 misc_feature 2092..2097 /label=10/25 N-part scar misc_feature 2092..2097 /label=BBa_K1159995 RBS 2098..2101 /label=Consensus Sequence for P. patens CDS 2102..2164 /label=Ig Kappa Signal Peptide misc_feature 2098..2164 /label=BBa_K1159304 misc_feature 2165..2170 /label=25/25 scar misc_feature 2165..2170 /label=BBa_K1159993 misc_feature 2171..2209 /label=SpyTag misc_feature 2189..2191 /label=Reactive Asp for isopeptide forming reaction CDS 2171..2209 /label=BBa_K1159201 CDS 2098..2209 /label=BBa_K1159203 misc_feature 2210..2215 /label=25/25 scar misc_feature 2210..2215 /label=BBa_K1159993 CDS 2216..2725 /label=NanoLuc Luciferase CDS 2216..2725 /label=BBa_K1159001 CDS 2098..2725 /label=BBa_K1159208 ORIGIN 1 aacaatgcct ggtgaagttg cttggtggag gcctcttttc cttatcgctc ttatgcctat 61 cggagtgctc tctaacgctg agggtgatgc tcttaacacc accggcgttg ataccttatc 121 aggtttatca agtgagcaag gtcagtccgg tgatatgaca attgaagaag atagtgctac 181 ccatattaaa ttctcaaaac gtgatgagga cggcaaagag ttagctggtg caactatgga 241 gttgcgtgat tcatctggta aaactattag tacatggatt tcagatggac aagtgaaaga 301 tttctacctg tatccaggaa aatatacatt tgtcgaaacc gcagcaccag acggttatga 361 ggtagcaact gctattacct ttacagttaa tgagcaaggt caggttactg taaatggcaa 421 agcaactaaa ggtgacgctc atattaccgg cagcgcttgg tctcatcctc aatttgaaaa 481 gggtgaaaat ctttattttc aaagcggaac cggccctgga caacctcctt ttcctcctcc 541 tcctcctttt acaccacctc ctccacaaac tcctaacggt gcttctggtg agaactctac 601 tggtgctatt gctggtggtg ttgctgctgg tgcagctctt ttgtttgctg ctcctgctat 661 tggattcgct tggtggagaa gaagaaggcc tattgaggct accggcggtg gttctggtgg 721 tggttctggt accggcagta aaggagaaga acttttcact ggagttgtcc caattcttgt 781 tgaattagat ggtgacgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga 841 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc 901 atggccaaca cttgtcacta ctctgactta tggtgttcaa tgcttttcaa gatacccaga 961 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag 1021 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg 1081 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat 1141 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa 1201 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt 1261 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tcctttcacc 1321 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga 1381 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgagct 1441 ctacaaaacc ggttaatact agagttaaaa cagctctggg gttgtaccca ccccagaggc 1501 ccacgtggcg gctagtactc cggtattgcg gtacccttgt acgcctgttt tatactccct 1561 tcccgtaact tagacgcaca aaaccaagtt caatagaagg gggtacaaac cagtaccacc 1621 acgaacaagc acttctgttt ccccggtgat gtcgtataga ctgcttgcgt ggttgaaagc 1681 gacggatccg ttatccgctt atgtacttcg agaagcccag taccacctcg gaatcttcga 1741 tgcgttgcgc tcagcactca accccagagt gtagcttagg ctgatgagtc tggacatccc 1801 tcaccggtga cggtggtcca ggctgcgttg gcggcctacc tatggctaac gccatgggac 1861 gctagttgtg aacaaggtgt gaagagccta ttgagctaca taagaatcct ccggcccctg 1921 aatgcggcta atcccaacct cggagcaggt ggtcacaaac cagtgattgg cctgtcgtaa 1981 cgcgcaagtc cgtggcggaa ccgactactt tgggtgtccg tgtttccttt tattttattg 2041 tggctgctta tggtgacaat cacagattgt tatcataaag cgaattggat ttactagaac 2101 aatggaaacc gatactctcc tcctctgggt tctcttgctt tgggttccag gatctaccgg 2161 cgataccggc gctcatattg tcatggttga tgcttacaag ccaactaaga ccggcgtgtt 2221 caccctcgag gatttcgttg gagattggag acagaccgct ggatacaacc ttgatcaggt 2281 tttggagcag ggtggtgtgt ctagtctttt ccagaacctc ggagtgtctg tgacccctat 2341 ccagagaatc gttctctctg gtgagaacgg actcaagatc gatatccacg tgatcatccc 2401 ttacgaggga ctctcaggtg atcagatggg acagatcgag aagattttca aggtggtgta 2461 ccctgttgat gatcaccact tcaaggtgat cctccactac ggaactctcg tgatcgatgg 2521 tgtgacccca aacatgatcg attacttcgg aaggccatac gagggaatcg ctgtgttcga 2581 tggaaagaag attaccgtta ccggaaccct ctggaacgga aacaagatta tcgatgagag 2641 gctcatcaac cctgatggat ctctcttgtt cagggtgacc atcaacggtg tgactggttg 2701 gagactttgc gagagaatcc ttgct //