##gff-version 3
##sequence-region BBa_K1159212 1 2725
BBa_K1159212	.	CDS	1	1447	.	+	0	ID=BBa_K1159206;Name=BBa_K1159206
BBa_K1159212	.	CDS	452	1447	.	+	0	ID=BBa_K1159316;Name=BBa_K1159316;Parent=BBa_K1159206
BBa_K1159212	.	polypeptide_domain	452	508	.	+	0	ID=BBa_K1159309;Name=BBa_K1159309;Parent=BBa_K1159316
BBa_K1159212	.	sequence_feature	485	505	.	+	0	ID=annotation2342591;Name=TEV cleavage site;Parent=BBa_K1159309
BBa_K1159212	.	tag	458	481	.	+	0	ID=annotation2342578;Name=''Strep''-tag II;Parent=BBa_K1159309
BBa_K1159212	.	sequence_feature	509	514	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993;Parent=BBa_K1159316
BBa_K1159212	.	sequence_feature	509	514	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159212	.	CDS	515	1447	.	+	0	ID=BBa_K1159315;Name=BBa_K1159315;Parent=BBa_K1159316
BBa_K1159212	.	polypeptide_domain	515	700	.	+	0	ID=BBa_K1159305;Name=BBa_K1159305;Parent=BBa_K1159315
BBa_K1159212	.	CDS	515	700	.	+	0	ID=annotation2343588;Name=Transmembrane Domain of SERK Receptor from ''P. patens'';Parent=BBa_K1159305
BBa_K1159212	.	CDS	737	1447	.	+	0	ID=BBa_K1159311;Name=BBa_K1159311;Parent=BBa_K1159315
BBa_K1159212	.	CDS	737	1447	.	+	0	ID=annotation2343590;Name=GFPmut1;Parent=BBa_K1159311
BBa_K1159212	.	sequence_feature	701	706	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993;Parent=BBa_K1159315
BBa_K1159212	.	sequence_feature	701	706	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159212	.	sequence_feature	707	730	.	+	0	ID=BBa_K243005;Name=BBa_K243005;Parent=BBa_K1159315
BBa_K1159212	.	engineered_region	707	730	.	+	0	ID=annotation2041884;Name=MiddleLinker;Parent=BBa_K243005
BBa_K1159212	.	sequence_feature	731	736	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993;Parent=BBa_K1159315
BBa_K1159212	.	sequence_feature	731	736	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159212	.	sequence_feature	446	451	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993;Parent=BBa_K1159206
BBa_K1159212	.	sequence_feature	446	451	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159212	.	CDS	1	445	.	+	0	ID=BBa_K1159204;Name=BBa_K1159204;Parent=BBa_K1159206
BBa_K1159212	.	polypeptide_domain	1	100	.	+	0	ID=BBa_K1159303;Name=BBa_K1159303;Parent=BBa_K1159204
BBa_K1159212	.	ribosome_entry_site	1	4	.	+	0	ID=annotation2341512;Name=Consensus Sequence for ''P. patens'';Parent=BBa_K1159303
BBa_K1159212	.	CDS	5	100	.	+	0	ID=annotation2341513;Name=SERK Signal Peptide;Parent=BBa_K1159303
BBa_K1159212	.	sequence_feature	101	106	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993;Parent=BBa_K1159204
BBa_K1159212	.	sequence_feature	101	106	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159212	.	CDS	107	445	.	+	0	ID=BBa_K1159200;Name=BBa_K1159200;Parent=BBa_K1159204
BBa_K1159212	.	sequence_alteration	206	208	.	+	0	ID=annotation2343348;Name=Iso --> Glu;Parent=BBa_K1159200
BBa_K1159212	.	CDS	107	445	.	+	0	ID=annotation2343345;Name=N-terminal FbaB (modified SpyCatcher);Parent=BBa_K1159200
BBa_K1159212	.	conserved_region	197	199	.	+	0	ID=annotation2343366;Name=Reactive Lys for isopeptide forming reaction;Parent=BBa_K1159200
BBa_K1159212	.	sequence_alteration	311	313	.	+	0	ID=annotation2343349;Name=Met --> Tyr;Parent=BBa_K1159200
BBa_K1159212	.	CDS	2098	2725	.	+	0	ID=BBa_K1159208;Name=BBa_K1159208
BBa_K1159212	.	sequence_feature	2210	2215	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993;Parent=BBa_K1159208
BBa_K1159212	.	sequence_feature	2210	2215	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159212	.	CDS	2216	2725	.	+	0	ID=BBa_K1159001;Name=BBa_K1159001;Parent=BBa_K1159208
BBa_K1159212	.	CDS	2216	2725	.	+	0	ID=annotation2342942;Name=NanoLuc Luciferase;Parent=BBa_K1159001
BBa_K1159212	.	CDS	2098	2209	.	+	0	ID=BBa_K1159203;Name=BBa_K1159203;Parent=BBa_K1159208
BBa_K1159212	.	CDS	2171	2209	.	+	0	ID=BBa_K1159201;Name=BBa_K1159201;Parent=BBa_K1159203
BBa_K1159212	.	tag	2171	2209	.	+	0	ID=annotation2343481;Name=SpyTag;Parent=BBa_K1159201
BBa_K1159212	.	conserved_region	2189	2191	.	+	0	ID=annotation2343512;Name=Reactive Asp for isopeptide forming reaction;Parent=BBa_K1159201
BBa_K1159212	.	polypeptide_domain	2098	2164	.	+	0	ID=BBa_K1159304;Name=BBa_K1159304;Parent=BBa_K1159203
BBa_K1159212	.	ribosome_entry_site	2098	2101	.	+	0	ID=annotation2341498;Name=Consensus Sequence for P. patens;Parent=BBa_K1159304
BBa_K1159212	.	CDS	2102	2164	.	+	0	ID=annotation2341499;Name=Ig Kappa Signal Peptide;Parent=BBa_K1159304
BBa_K1159212	.	sequence_feature	2165	2170	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993;Parent=BBa_K1159203
BBa_K1159212	.	sequence_feature	2165	2170	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159212	.	sequence_feature	1448	1464	.	+	0	ID=BBa_K1159996;Name=BBa_K1159996
BBa_K1159212	.	sequence_feature	1448	1464	.	+	0	ID=annotation2342869;Name=25/10 NC scar;Parent=BBa_K1159996
BBa_K1159212	.	ribosome_entry_site	1465	2091	.	+	0	ID=BBa_K1159300;Name=BBa_K1159300
BBa_K1159212	.	ribosome_entry_site	1465	2091	.	+	0	ID=annotation2343585;Name=Polioviral IRES;Parent=BBa_K1159300
BBa_K1159212	.	sequence_feature	2092	2097	.	+	0	ID=BBa_K1159995;Name=BBa_K1159995
BBa_K1159212	.	sequence_feature	2092	2097	.	+	0	ID=annotation2342867;Name=10/25 N-part scar;Parent=BBa_K1159995
>BBa_K1159212
aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgct
gagggtgatgctcttaacaccaccggcgttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatga
caattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactat
ggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatcca
ggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagc
aaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatattaccggcagcgcttggtctcatcctcaatt
tgaaaagggtgaaaatctttattttcaaagcggaaccggccctggacaacctccttttcctcctcctcctccttttaca
ccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtg
cagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggctaccggcggtgg
ttctggtggtggttctggtaccggcagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagat
ggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaat
ttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatggtgttcaatgcttttc
aagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactata
tttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagt
taaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtata
catcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaa
ctagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaaccattacctgtccacac
aatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattac
acatggcatggatgagctctacaaaaccggttaatactagagttaaaacagctctggggttgtacccaccccagaggcc
cacgtggcggctagtactccggtattgcggtacccttgtacgcctgttttatactcccttcccgtaacttagacgcaca
aaaccaagttcaatagaagggggtacaaaccagtaccaccacgaacaagcacttctgtttccccggtgatgtcgtatag
actgcttgcgtggttgaaagcgacggatccgttatccgcttatgtacttcgagaagcccagtaccacctcggaatcttc
gatgcgttgcgctcagcactcaaccccagagtgtagcttaggctgatgagtctggacatccctcaccggtgacggtggt
ccaggctgcgttggcggcctacctatggctaacgccatgggacgctagttgtgaacaaggtgtgaagagcctattgagc
tacataagaatcctccggcccctgaatgcggctaatcccaacctcggagcaggtggtcacaaaccagtgattggcctgt
cgtaacgcgcaagtccgtggcggaaccgactactttgggtgtccgtgtttccttttattttattgtggctgcttatggt
gacaatcacagattgttatcataaagcgaattggatttactagaacaatggaaaccgatactctcctcctctgggttct
cttgctttgggttccaggatctaccggcgataccggcgctcatattgtcatggttgatgcttacaagccaactaagacc
ggcgtgttcaccctcgaggatttcgttggagattggagacagaccgctggatacaaccttgatcaggttttggagcagg
gtggtgtgtctagtcttttccagaacctcggagtgtctgtgacccctatccagagaatcgttctctctggtgagaacgg
actcaagatcgatatccacgtgatcatcccttacgagggactctcaggtgatcagatgggacagatcgagaagattttc
aaggtggtgtaccctgttgatgatcaccacttcaaggtgatcctccactacggaactctcgtgatcgatggtgtgaccc
caaacatgatcgattacttcggaaggccatacgagggaatcgctgtgttcgatggaaagaagattaccgttaccggaac
cctctggaacggaaacaagattatcgatgagaggctcatcaaccctgatggatctctcttgttcagggtgaccatcaac
ggtgtgactggttggagactttgcgagagaatccttgct