##gff-version 3
##sequence-region BBa_K1159214 1 2725
BBa_K1159214	.	sequence_feature	1148	1164	.	+	0	ID=BBa_K1159996;Name=BBa_K1159996
BBa_K1159214	.	sequence_feature	1148	1164	.	+	0	ID=annotation2342869;Name=25/10 NC scar;Parent=BBa_K1159996
BBa_K1159214	.	ribosome_entry_site	1165	1791	.	+	0	ID=BBa_K1159300;Name=BBa_K1159300
BBa_K1159214	.	ribosome_entry_site	1165	1791	.	+	0	ID=annotation2343585;Name=Polioviral IRES;Parent=BBa_K1159300
BBa_K1159214	.	CDS	1798	2725	.	+	0	ID=BBa_K1159210;Name=BBa_K1159210
BBa_K1159214	.	sequence_feature	2381	2386	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993;Parent=BBa_K1159210
BBa_K1159214	.	sequence_feature	2381	2386	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159214	.	CDS	1798	2380	.	+	0	ID=BBa_K1159006;Name=BBa_K1159006;Parent=BBa_K1159210
BBa_K1159214	.	sequence_feature	1865	1870	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993;Parent=BBa_K1159006
BBa_K1159214	.	sequence_feature	1865	1870	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159214	.	CDS	1871	2380	.	+	0	ID=BBa_K1159001;Name=BBa_K1159001;Parent=BBa_K1159006
BBa_K1159214	.	CDS	1871	2380	.	+	0	ID=annotation2342942;Name=NanoLuc Luciferase;Parent=BBa_K1159001
BBa_K1159214	.	polypeptide_domain	1798	1864	.	+	0	ID=BBa_K1159304;Name=BBa_K1159304;Parent=BBa_K1159006
BBa_K1159214	.	CDS	1802	1864	.	+	0	ID=annotation2341499;Name=Ig Kappa Signal Peptide;Parent=BBa_K1159304
BBa_K1159214	.	ribosome_entry_site	1798	1801	.	+	0	ID=annotation2341498;Name=Consensus Sequence for P. patens;Parent=BBa_K1159304
BBa_K1159214	.	CDS	2387	2725	.	+	0	ID=BBa_K1159200;Name=BBa_K1159200;Parent=BBa_K1159210
BBa_K1159214	.	conserved_region	2477	2479	.	+	0	ID=annotation2343366;Name=Reactive Lys for isopeptide forming reaction;Parent=BBa_K1159200
BBa_K1159214	.	CDS	2387	2725	.	+	0	ID=annotation2343345;Name=N-terminal FbaB (modified SpyCatcher);Parent=BBa_K1159200
BBa_K1159214	.	sequence_alteration	2486	2488	.	+	0	ID=annotation2343348;Name=Iso --> Glu;Parent=BBa_K1159200
BBa_K1159214	.	sequence_alteration	2591	2593	.	+	0	ID=annotation2343349;Name=Met --> Tyr;Parent=BBa_K1159200
BBa_K1159214	.	CDS	1	1147	.	+	0	ID=BBa_K1159207;Name=BBa_K1159207
BBa_K1159214	.	CDS	152	1147	.	+	0	ID=BBa_K1159316;Name=BBa_K1159316;Parent=BBa_K1159207
BBa_K1159214	.	polypeptide_domain	152	208	.	+	0	ID=BBa_K1159309;Name=BBa_K1159309;Parent=BBa_K1159316
BBa_K1159214	.	tag	158	181	.	+	0	ID=annotation2342578;Name=''Strep''-tag II;Parent=BBa_K1159309
BBa_K1159214	.	sequence_feature	185	205	.	+	0	ID=annotation2342591;Name=TEV cleavage site;Parent=BBa_K1159309
BBa_K1159214	.	sequence_feature	209	214	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993;Parent=BBa_K1159316
BBa_K1159214	.	sequence_feature	209	214	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159214	.	CDS	215	1147	.	+	0	ID=BBa_K1159315;Name=BBa_K1159315;Parent=BBa_K1159316
BBa_K1159214	.	CDS	437	1147	.	+	0	ID=BBa_K1159311;Name=BBa_K1159311;Parent=BBa_K1159315
BBa_K1159214	.	CDS	437	1147	.	+	0	ID=annotation2343590;Name=GFPmut1;Parent=BBa_K1159311
BBa_K1159214	.	polypeptide_domain	215	400	.	+	0	ID=BBa_K1159305;Name=BBa_K1159305;Parent=BBa_K1159315
BBa_K1159214	.	CDS	215	400	.	+	0	ID=annotation2343588;Name=Transmembrane Domain of SERK Receptor from ''P. patens'';Parent=BBa_K1159305
BBa_K1159214	.	sequence_feature	407	430	.	+	0	ID=BBa_K243005;Name=BBa_K243005;Parent=BBa_K1159315
BBa_K1159214	.	engineered_region	407	430	.	+	0	ID=annotation2041884;Name=MiddleLinker;Parent=BBa_K243005
BBa_K1159214	.	sequence_feature	401	406	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993;Parent=BBa_K1159315
BBa_K1159214	.	sequence_feature	401	406	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159214	.	sequence_feature	431	436	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993;Parent=BBa_K1159315
BBa_K1159214	.	sequence_feature	431	436	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159214	.	sequence_feature	146	151	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993;Parent=BBa_K1159207
BBa_K1159214	.	sequence_feature	146	151	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159214	.	CDS	1	145	.	+	0	ID=BBa_K1159205;Name=BBa_K1159205;Parent=BBa_K1159207
BBa_K1159214	.	sequence_feature	101	106	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993;Parent=BBa_K1159205
BBa_K1159214	.	sequence_feature	101	106	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159214	.	CDS	107	145	.	+	0	ID=BBa_K1159201;Name=BBa_K1159201;Parent=BBa_K1159205
BBa_K1159214	.	tag	107	145	.	+	0	ID=annotation2343481;Name=SpyTag;Parent=BBa_K1159201
BBa_K1159214	.	conserved_region	125	127	.	+	0	ID=annotation2343512;Name=Reactive Asp for isopeptide forming reaction;Parent=BBa_K1159201
BBa_K1159214	.	polypeptide_domain	1	100	.	+	0	ID=BBa_K1159303;Name=BBa_K1159303;Parent=BBa_K1159205
BBa_K1159214	.	ribosome_entry_site	1	4	.	+	0	ID=annotation2341512;Name=Consensus Sequence for ''P. patens'';Parent=BBa_K1159303
BBa_K1159214	.	CDS	5	100	.	+	0	ID=annotation2341513;Name=SERK Signal Peptide;Parent=BBa_K1159303
BBa_K1159214	.	sequence_feature	1792	1797	.	+	0	ID=BBa_K1159995;Name=BBa_K1159995
BBa_K1159214	.	sequence_feature	1792	1797	.	+	0	ID=annotation2342867;Name=10/25 N-part scar;Parent=BBa_K1159995
>BBa_K1159214
aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgct
gagggtgatgctcttaacaccaccggcgctcatattgtcatggttgatgcttacaagccaactaagaccggcagcgctt
ggtctcatcctcaatttgaaaagggtgaaaatctttattttcaaagcggaaccggccctggacaacctccttttcctcc
tcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggt
ggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattg
aggctaccggcggtggttctggtggtggttctggtaccggcagtaaaggagaagaacttttcactggagttgtcccaat
tcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacgga
aaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatg
gtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgt
acaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgataccctt
gttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactata
actcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattga
agatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaac
cattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaa
cagctgctgggattacacatggcatggatgagctctacaaaaccggttaatactagagttaaaacagctctggggttgt
acccaccccagaggcccacgtggcggctagtactccggtattgcggtacccttgtacgcctgttttatactcccttccc
gtaacttagacgcacaaaaccaagttcaatagaagggggtacaaaccagtaccaccacgaacaagcacttctgtttccc
cggtgatgtcgtatagactgcttgcgtggttgaaagcgacggatccgttatccgcttatgtacttcgagaagcccagta
ccacctcggaatcttcgatgcgttgcgctcagcactcaaccccagagtgtagcttaggctgatgagtctggacatccct
caccggtgacggtggtccaggctgcgttggcggcctacctatggctaacgccatgggacgctagttgtgaacaaggtgt
gaagagcctattgagctacataagaatcctccggcccctgaatgcggctaatcccaacctcggagcaggtggtcacaaa
ccagtgattggcctgtcgtaacgcgcaagtccgtggcggaaccgactactttgggtgtccgtgtttccttttattttat
tgtggctgcttatggtgacaatcacagattgttatcataaagcgaattggatttactagaacaatggaaaccgatactc
tcctcctctgggttctcttgctttgggttccaggatctaccggcgataccggcgtgttcaccctcgaggatttcgttgg
agattggagacagaccgctggatacaaccttgatcaggttttggagcagggtggtgtgtctagtcttttccagaacctc
ggagtgtctgtgacccctatccagagaatcgttctctctggtgagaacggactcaagatcgatatccacgtgatcatcc
cttacgagggactctcaggtgatcagatgggacagatcgagaagattttcaaggtggtgtaccctgttgatgatcacca
cttcaaggtgatcctccactacggaactctcgtgatcgatggtgtgaccccaaacatgatcgattacttcggaaggcca
tacgagggaatcgctgtgttcgatggaaagaagattaccgttaccggaaccctctggaacggaaacaagattatcgatg
agaggctcatcaaccctgatggatctctcttgttcagggtgaccatcaacggtgtgactggttggagactttgcgagag
aatccttgctaccggcgttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaa
gatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtg
attcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatac
atttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggtt
actgtaaatggcaaagcaactaaaggtgacgctcatatt