BBa_K1159205 1 BBa_K1159205 Secretory SpyTag (SERK-SigP_SpyTag) in RFC[25] N-Part 2013-09-16T11:00:00Z 2015-06-11T01:59:37Z Synthetic This part encodes the SpyTag [http://parts.igem.org/Part:BBa_K1159201 BBa_K1159201] with a N-terminal signal peptide for secretion (SERK receptor signal peptide from ''Physcomitrella patens''). This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible. false false _1471_ 4206 11507 9 In stock false x false Dong-Jiunn Jeffery TRUONG component2348900 1 BBa_K1159993 component2348898 1 BBa_K1159303 component2348903 1 BBa_K1159201 annotation2348900 1 BBa_K1159993 range2348900 1 101 106 annotation2348903 1 BBa_K1159201 range2348903 1 107 145 annotation2348898 1 BBa_K1159303 range2348898 1 1 100 BBa_K1159311 1 BBa_K1159311 GFPmut1 2013-09-14T11:00:00Z 2015-05-08T01:09:31Z ''Aequarea victoria'' This part codes for GFPmut1 which is flanked by RFC[25] pre- and suffix. false false _1471_ 0 11507 9 Not in stock false x false TU Munich 2013 annotation2343590 1 GFPmut1 range2343590 1 1 711 BBa_K1159309 1 BBa_K1159309 19 AA Linker with N-terminal <i>Strep</i>-tag II and C-terminal TEV cleavage site in RFC[25] 2013-09-14T11:00:00Z 2015-05-08T01:09:31Z Synthetic This part encodes a 19 amino acids long linker with a N-terminal ''Strep''-tag II and a C-terminal cleavage site for the TEV Protease. For further protein fusions this part is flanked by RFC[25} pre- and suffix. false false _1471_ 0 11507 9 In stock false x false TU Munich 2013 annotation2342591 1 TEV cleavage site range2342591 1 34 54 annotation2342578 1 ''Strep''-tag II range2342578 1 7 30 BBa_K1159001 1 BBa_K1159001 NanoLuc Luciferase in RFC[25] 2013-05-16T11:00:00Z 2016-01-26T02:12:53Z x x false false _1471_ 4206 14732 9 In stock true x false TU Munich 2013 annotation2342942 1 NanoLuc Luciferase range2342942 1 1 510 BBa_K1159207 1 BBa_K1159207 Membrane-anchored SpyTag in RFC[25] N-Part 2013-09-16T11:00:00Z 2015-06-11T01:58:39Z Synthetic This part codes for a membrane-anchored version of SpyTag [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159201 BBa_K1159201] for the chassis ''Physcomitrella patens''. Please see subparts for for further description. This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible. false false _1471_ 4206 11507 9 In stock false x false Dong-Jiunn Jeffery TRUONG component2349003 1 BBa_K1159316 component2348984 1 BBa_K1159205 component2348986 1 BBa_K1159993 annotation2348984 1 BBa_K1159205 range2348984 1 1 145 annotation2349003 1 BBa_K1159316 range2349003 1 152 1147 annotation2348986 1 BBa_K1159993 range2348986 1 146 151 BBa_K1159993 1 BBa_K1159993 25/25 Assembly Scar 2013-09-14T11:00:00Z 2015-05-08T01:09:31Z Synthetic Assembly scar between RFC[25] Biobrick and RFC[25] Biobrick. false false _1471_ 0 11507 9 Not in stock false x false TU Munich 2013 annotation2342863 1 25/25 scar range2342863 1 1 6 BBa_K1159995 1 BBa_K1159995 10/25 N-Part Assembly Scar 2013-09-14T11:00:00Z 2015-05-08T01:09:31Z Synthetic Assembly scar between RFC[10] coding/non-coding Biobrick and RFC[25] N-part Biobrick (not RFC[25] standard prefix!). Formally it has the same scar like the assembly scar between RFC[10] coding/non-coding Biobrick and RFC[10] coding Biobrick. false false _1471_ 0 11507 9 Not in stock false x false TU Munich 2013 annotation2342867 1 10/25 N-part scar range2342867 1 1 6 BBa_K1159303 1 BBa_K1159303 Signal Peptide of SERK Receptor from <i>Physcomitrella patens</i> in RFC[25] 2013-09-13T11:00:00Z 2015-06-11T01:54:49Z ''Physcomitrella patens'' This part codes for the signal peptide from the SERK receptor (Somatic Embryogenesis RECEPTOR KINAS) of ''Physocmitrella patens''. The signal peptide induces translocation of the protein into the Endoplasmatic Reticulum (ER) for secretion or membrane integration. Protein fused to the C-terminus of this part should be secreted or otherwise if the part fused region contains a transmembrane domain it will be integrated in cytoplasmatic membrane. This part starts with a consensus sequence for ''Physcomitrella patens'' which allows efficient translation start. Furthermore, this part is flanked by RFC[25] N-part pre- and suffix for fusions to the C-terminus of this part. false false _1471_ 4206 11507 9 In stock false x false TU Munich 2013 annotation2341512 1 Consensus Sequence for ''P. patens'' range2341512 1 1 4 annotation2341513 1 SERK Signal Peptide range2341513 1 5 100 BBa_K1159305 1 BBa_K1159305 Transmembrane domain of SERK Receptor from <i>Physcomitrella patens</i> in RFC[25] 2013-09-13T11:00:00Z 2015-06-11T01:54:11Z ''Physcomitrella patens'' This part is encoding the transmembrane region of the SERK Receptor from ''Physcomitrella patens''. For membrane translocation a N-terminal signal peptide is still necessary for translocation through the ER membrane. This part is flanked by RFC[25] pre- and suffix. false false _1471_ 4206 11507 9 In stock false x false TU Munich 2013 annotation2343588 1 Transmembrane Domain of SERK Receptor from ''P. patens'' range2343588 1 1 186 BBa_K1159315 1 BBa_K1159315 TMD of SERK Receptor from <i>Physcomitrella patens</i> with C-terminal GFP fusion in RFC[25] 2013-09-15T11:00:00Z 2015-05-28T03:06:34Z Synthetic This part codes for the Transmembrane Domain of SERK Receptor from <i>Physcomitrella patens'' with a C-terminal (intracellular) GFPmut1 fusion; SERK TMD and GFPmut1 are seperated by a middle long linker. Still, a signal peptide is needed for membrane integration. This part is flanked by RFC[25] pre- and suffix for further protein fusions. false false _1471_ 4206 11507 9 In stock false x false TU Munich 2013 component2346338 1 BBa_K243005 component2346334 1 BBa_K1159305 component2346340 1 BBa_K1159993 component2346336 1 BBa_K1159993 component2346342 1 BBa_K1159311 annotation2346342 1 BBa_K1159311 range2346342 1 223 933 annotation2346340 1 BBa_K1159993 range2346340 1 217 222 annotation2346338 1 BBa_K243005 range2346338 1 193 216 annotation2346336 1 BBa_K1159993 range2346336 1 187 192 annotation2346334 1 BBa_K1159305 range2346334 1 1 186 BBa_K1159200 1 BBa_K1159200 Splitted and engineered N-terminal FbaB for isopeptide bound formation (SpyCatcher) in RFC[25] 2013-09-13T11:00:00Z 2015-05-08T01:09:31Z ''Streptococcus pyogenes'' This part codes for a protein that recognizes and forms a covalent isopeptide bound to a oligopeptide. That oligopeptide can be found under BBa_K1159201. This part is flanked by RFC[25] pre- and suffix for further protein fusions. false false _1471_ 0 11507 9 In stock false x false Dong-Jiunn Jeffery TRUONG annotation2343366 1 Reactive Lys for isopeptide forming reaction range2343366 1 91 93 annotation2343345 1 N-terminal FbaB (modified SpyCatcher) range2343345 1 1 339 annotation2343348 1 Iso --> Glu range2343348 1 100 102 annotation2343349 1 Met --> Tyr range2343349 1 205 207 BBa_K1159006 1 BBa_K1159006 Secretory NanoLuc Luciferase (IgKappa-SigP_nLuc) in RFC[25] N-Part 2013-09-14T11:00:00Z 2015-05-28T03:52:04Z Synthetic NanoLuc Luciferase is engineered ATP-independent luciferase from a deep-sea shrimp which luminescense 2 magnitues higher than these from Renilla reniformis or from Photinus pyralis (firefly). Also the molecular weight of NanoLuc luciferase is twice smaller compared to other luciferase (only 19 kDa). This part encodes for a NanoLuc Luciferase which is fused to a N-terminal signal peptide for secretion (IgKappa signal peptide from Mus musculus) with also works in moss (Physcomitrella patens). This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible. false false _1471_ 4206 11507 9 In stock false x false TU Munich 2013 component2343010 1 BBa_K1159001 component2343006 1 BBa_K1159304 component2343008 1 BBa_K1159993 annotation2343006 1 BBa_K1159304 range2343006 1 1 67 annotation2343008 1 BBa_K1159993 range2343008 1 68 73 annotation2343010 1 BBa_K1159001 range2343010 1 74 583 BBa_K1159304 1 BBa_K1159304 Signal Peptide of Ig Kappa chain from <i>Mus musculus</i> in RFC[25] N-part 2013-09-13T11:00:00Z 2015-06-11T01:54:39Z ''Mus musculus'' This part codes for the signal peptide of the Ig Kappa chain from ''Mus musculus''. The signal peptide induces translocation of the protein into the Endoplasmatic Reticulum (ER) for secretion or membrane integration. Protein fused to the C-terminus of this part should be secreted or otherwise if the part fused region contains a transmembrane domain it will be integrated in cytoplasmatic membrane. This part starts with a consensus sequence for Physcomitrella patens which allows efficient translation start. Furthermore, this part is flanked by RFC[25] N-part pre- and suffix for fusions to the C-terminus of this part. false false _1471_ 4206 11507 9 In stock false x false TU Munich 2013 annotation2341499 1 Ig Kappa Signal Peptide range2341499 1 5 67 annotation2341498 1 Consensus Sequence for P. patens range2341498 1 1 4 BBa_K1159210 1 BBa_K1159210 Secretory nLuc_SpyCatcher fusion (IgKappa-SigP_nLuc_SpyCatcher) in RFC[25] N-Part 2013-09-16T11:00:00Z 2015-05-08T01:09:31Z Synthetic Secretory Fusionprotein consisting N-terminal nLuc and C-terminal SpyTag (IgKappa-SigP_nLuc_SpyTag) in RFC[25] N-Part. This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible. false false _1471_ 0 11507 9 In stock false x false Dong-Jiunn Jeffery TRUONG component2350301 1 BBa_K1159200 component2350294 1 BBa_K1159006 component2350296 1 BBa_K1159993 annotation2350296 1 BBa_K1159993 range2350296 1 584 589 annotation2350294 1 BBa_K1159006 range2350294 1 1 583 annotation2350301 1 BBa_K1159200 range2350301 1 590 928 BBa_K1159300 1 BBa_K1159300 Polioviral Internal Ribosome Entry Site (IRES) for polycistronic expression in eukaryotes 2013-09-13T11:00:00Z 2015-06-11T01:55:50Z Human Poliovirus 1 Mahoney Strain This part contains the Internal Ribosome Entry Site (IRES) of the human poliovirus 1 Mahoney strain which allows polycistronic expression systems in eukaryotic systems. This part is flanked by RFC[10] pre- and suffix. By cloning this part betweens CDSs, many proteins/peptides can expressed by a single promoter and terminator. false false _1471_ 4206 11507 9 In stock false x false Dong-Jiunn Jeffery TRUONG annotation2343585 1 Polioviral IRES range2343585 1 1 627 BBa_K1159201 1 BBa_K1159201 Splitted and engineered C-terminal FbaB for isopeptide bound formation (SpyTag) in RFC[25] 2013-09-13T11:00:00Z 2015-05-08T01:09:31Z ''Streptococcus pyogenes'' This part codes for a oligopeptide that is recognized and bound by a covalent isopeptide bound to a protein, called SpyCatcher. That catcher protein for this oligopeptdie can be found under BBa_K1159200. This part is flanked by RFC[25] pre- and suffix for further protein fusions. false false _1471_ 0 11507 9 In stock false x false Dong-Jiunn Jeffery TRUONG annotation2343481 1 SpyTag range2343481 1 1 39 annotation2343512 1 Reactive Asp for isopeptide forming reaction range2343512 1 19 21 BBa_K1159214 1 BBa_K1159214 Membrane-anchored SpyTag with secretory nLuc_SpyCatcher reporter fusion translational unit 2013-09-16T11:00:00Z 2015-05-08T01:09:31Z Synthetic This part encodes a translational unit consisting two fusion proteins, a Membrane-anchored SpyCatcher and a secretory nLuc_SpyTag (IgKappa-SigP_nLuc_SpyTag) reporter fusion to show if post-translational protein fusion like reaction happens between the membrane-localized catcher protein and the secretory fusion protein containing N-terminal nLuc and C-terminal SpyTag Luciferase. As a translational unit both fusion proteins are seperated by a polioviral IRES to allow bicistronic expression in eukaryotic systems. To express this system you only have to insert your favored promoter/terminator in the prefix/suffix of this part. For further information, please see under the subparts linked in the text. false false _1471_ 0 11507 9 In stock false x false Dong-Jiunn Jeffery TRUONG component2350872 1 BBa_K1159210 component2350854 1 BBa_K1159300 component2350856 1 BBa_K1159995 component2350850 1 BBa_K1159207 component2350852 1 BBa_K1159996 annotation2350850 1 BBa_K1159207 range2350850 1 1 1147 annotation2350872 1 BBa_K1159210 range2350872 1 1798 2725 annotation2350852 1 BBa_K1159996 range2350852 1 1148 1164 annotation2350854 1 BBa_K1159300 range2350854 1 1165 1791 annotation2350856 1 BBa_K1159995 range2350856 1 1792 1797 BBa_K1159316 1 BBa_K1159316 SERK-Receptor TMD with C-terminal GFP and N-terminal <i>Strep</i>-tag II TEV-site linker in RFC[25] 2013-09-15T11:00:00Z 2015-06-11T01:53:10Z Synthetic This part codes for the Transmembrane Domain of SERK Receptor from ''Physcomitrella patens'' with a C-terminal (intracellular) GFPmut1 fusion and a N-terminal fusion to a linker containing ''Strep''-tag II and a TEV cleavage site; SERK TMD and GFPmut1 are seperated by a middle long linker. This part is flanked by RFC[25] pre- and suffix for further protein fusions. Note: A signal peptide is needed for membrane integration. false false _1471_ 4206 11507 9 In stock false x false TU Munich 2013 component2346350 1 BBa_K1159309 component2346352 1 BBa_K1159993 component2346363 1 BBa_K1159315 annotation2346352 1 BBa_K1159993 range2346352 1 58 63 annotation2346363 1 BBa_K1159315 range2346363 1 64 996 annotation2346350 1 BBa_K1159309 range2346350 1 1 57 BBa_K243005 1 MiddleLink Middle Linker ( Gly-Gly-Ser-Gly)x2 2009-10-13T11:00:00Z 2015-05-08T01:11:37Z Oligos synthesized by sigma. Hybridized by PCR. This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. We used this part to connect our protein domains with our lipocalin domains, for our project the universal endonuclease.The sequence produced the aminoacids Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly . false false _352_ 0 4732 9 It's complicated false None. false Freiburg Bioware09 annotation2041884 1 MiddleLinker range2041884 1 1 24 BBa_K1159996 1 BBa_K1159996 25/10 NC Assembly Scar 2013-09-14T11:00:00Z 2015-05-08T01:09:31Z Synthetic Assembly scar between RFC[25] Biobrick and RFC[10] non-coding Biobrick. false false _1471_ 0 11507 9 Not in stock false x false TU Munich 2013 annotation2342869 1 25/10 NC scar range2342869 1 1 17 BBa_K1159993_sequence 1 accggc BBa_K1159300_sequence 1 ttaaaacagctctggggttgtacccaccccagaggcccacgtggcggctagtactccggtattgcggtacccttgtacgcctgttttatactcccttcccgtaacttagacgcacaaaaccaagttcaatagaagggggtacaaaccagtaccaccacgaacaagcacttctgtttccccggtgatgtcgtatagactgcttgcgtggttgaaagcgacggatccgttatccgcttatgtacttcgagaagcccagtaccacctcggaatcttcgatgcgttgcgctcagcactcaaccccagagtgtagcttaggctgatgagtctggacatccctcaccggtgacggtggtccaggctgcgttggcggcctacctatggctaacgccatgggacgctagttgtgaacaaggtgtgaagagcctattgagctacataagaatcctccggcccctgaatgcggctaatcccaacctcggagcaggtggtcacaaaccagtgattggcctgtcgtaacgcgcaagtccgtggcggaaccgactactttgggtgtccgtgtttccttttattttattgtggctgcttatggtgacaatcacagattgttatcataaagcgaattggatt BBa_K1159304_sequence 1 aacaatggaaaccgatactctcctcctctgggttctcttgctttgggttccaggatctaccggcgat BBa_K1159006_sequence 1 aacaatggaaaccgatactctcctcctctgggttctcttgctttgggttccaggatctaccggcgataccggcgtgttcaccctcgaggatttcgttggagattggagacagaccgctggatacaaccttgatcaggttttggagcagggtggtgtgtctagtcttttccagaacctcggagtgtctgtgacccctatccagagaatcgttctctctggtgagaacggactcaagatcgatatccacgtgatcatcccttacgagggactctcaggtgatcagatgggacagatcgagaagattttcaaggtggtgtaccctgttgatgatcaccacttcaaggtgatcctccactacggaactctcgtgatcgatggtgtgaccccaaacatgatcgattacttcggaaggccatacgagggaatcgctgtgttcgatggaaagaagattaccgttaccggaaccctctggaacggaaacaagattatcgatgagaggctcatcaaccctgatggatctctcttgttcagggtgaccatcaacggtgtgactggttggagactttgcgagagaatccttgct BBa_K1159200_sequence 1 gttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatatt BBa_K1159303_sequence 1 aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacacc BBa_K243005_sequence 1 ggtggttctggtggtggttctggt BBa_K1159205_sequence 1 aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacaccaccggcgctcatattgtcatggttgatgcttacaagccaactaag BBa_K1159996_sequence 1 accggttaatactagag BBa_K1159201_sequence 1 gctcatattgtcatggttgatgcttacaagccaactaag BBa_K1159995_sequence 1 tactag BBa_K1159207_sequence 1 aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacaccaccggcgctcatattgtcatggttgatgcttacaagccaactaagaccggcagcgcttggtctcatcctcaatttgaaaagggtgaaaatctttattttcaaagcggaaccggccctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggctaccggcggtggttctggtggtggttctggtaccggcagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgagctctacaaa BBa_K1159315_sequence 1 cctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggctaccggcggtggttctggtggtggttctggtaccggcagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgagctctacaaa BBa_K1159001_sequence 1 gtgttcaccctcgaggatttcgttggagattggagacagaccgctggatacaaccttgatcaggttttggagcagggtggtgtgtctagtcttttccagaacctcggagtgtctgtgacccctatccagagaatcgttctctctggtgagaacggactcaagatcgatatccacgtgatcatcccttacgagggactctcaggtgatcagatgggacagatcgagaagattttcaaggtggtgtaccctgttgatgatcaccacttcaaggtgatcctccactacggaactctcgtgatcgatggtgtgaccccaaacatgatcgattacttcggaaggccatacgagggaatcgctgtgttcgatggaaagaagattaccgttaccggaaccctctggaacggaaacaagattatcgatgagaggctcatcaaccctgatggatctctcttgttcagggtgaccatcaacggtgtgactggttggagactttgcgagagaatccttgct BBa_K1159214_sequence 1 aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacaccaccggcgctcatattgtcatggttgatgcttacaagccaactaagaccggcagcgcttggtctcatcctcaatttgaaaagggtgaaaatctttattttcaaagcggaaccggccctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggctaccggcggtggttctggtggtggttctggtaccggcagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgagctctacaaaaccggttaatactagagttaaaacagctctggggttgtacccaccccagaggcccacgtggcggctagtactccggtattgcggtacccttgtacgcctgttttatactcccttcccgtaacttagacgcacaaaaccaagttcaatagaagggggtacaaaccagtaccaccacgaacaagcacttctgtttccccggtgatgtcgtatagactgcttgcgtggttgaaagcgacggatccgttatccgcttatgtacttcgagaagcccagtaccacctcggaatcttcgatgcgttgcgctcagcactcaaccccagagtgtagcttaggctgatgagtctggacatccctcaccggtgacggtggtccaggctgcgttggcggcctacctatggctaacgccatgggacgctagttgtgaacaaggtgtgaagagcctattgagctacataagaatcctccggcccctgaatgcggctaatcccaacctcggagcaggtggtcacaaaccagtgattggcctgtcgtaacgcgcaagtccgtggcggaaccgactactttgggtgtccgtgtttccttttattttattgtggctgcttatggtgacaatcacagattgttatcataaagcgaattggatttactagaacaatggaaaccgatactctcctcctctgggttctcttgctttgggttccaggatctaccggcgataccggcgtgttcaccctcgaggatttcgttggagattggagacagaccgctggatacaaccttgatcaggttttggagcagggtggtgtgtctagtcttttccagaacctcggagtgtctgtgacccctatccagagaatcgttctctctggtgagaacggactcaagatcgatatccacgtgatcatcccttacgagggactctcaggtgatcagatgggacagatcgagaagattttcaaggtggtgtaccctgttgatgatcaccacttcaaggtgatcctccactacggaactctcgtgatcgatggtgtgaccccaaacatgatcgattacttcggaaggccatacgagggaatcgctgtgttcgatggaaagaagattaccgttaccggaaccctctggaacggaaacaagattatcgatgagaggctcatcaaccctgatggatctctcttgttcagggtgaccatcaacggtgtgactggttggagactttgcgagagaatccttgctaccggcgttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatatt BBa_K1159309_sequence 1 agcgcttggtctcatcctcaatttgaaaagggtgaaaatctttattttcaaagcgga BBa_K1159311_sequence 1 agtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgagctctacaaa BBa_K1159305_sequence 1 cctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggct BBa_K1159210_sequence 1 aacaatggaaaccgatactctcctcctctgggttctcttgctttgggttccaggatctaccggcgataccggcgtgttcaccctcgaggatttcgttggagattggagacagaccgctggatacaaccttgatcaggttttggagcagggtggtgtgtctagtcttttccagaacctcggagtgtctgtgacccctatccagagaatcgttctctctggtgagaacggactcaagatcgatatccacgtgatcatcccttacgagggactctcaggtgatcagatgggacagatcgagaagattttcaaggtggtgtaccctgttgatgatcaccacttcaaggtgatcctccactacggaactctcgtgatcgatggtgtgaccccaaacatgatcgattacttcggaaggccatacgagggaatcgctgtgttcgatggaaagaagattaccgttaccggaaccctctggaacggaaacaagattatcgatgagaggctcatcaaccctgatggatctctcttgttcagggtgaccatcaacggtgtgactggttggagactttgcgagagaatccttgctaccggcgttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatatt BBa_K1159316_sequence 1 agcgcttggtctcatcctcaatttgaaaagggtgaaaatctttattttcaaagcggaaccggccctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggctaccggcggtggttctggtggtggttctggtaccggcagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgagctctacaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z