LOCUS BBa_K1159215 2725 bp DNA linear 25-Nov-2024 DEFINITION Membrane-anchored SpyTag with secretory SpyCatcher_nLuc reporter fusion translational unit ACCESSION BBa_K1159215 VERSION BBa_K1159215.1 FEATURES Location/Qualifiers RBS 1..4 /label=Consensus Sequence for ''P. patens'' CDS 5..100 /label=SERK Signal Peptide misc_feature 1..100 /label=BBa_K1159303 misc_feature 101..106 /label=25/25 scar misc_feature 101..106 /label=BBa_K1159993 misc_feature 107..145 /label=SpyTag misc_feature 125..127 /label=Reactive Asp for isopeptide forming reaction CDS 107..145 /label=BBa_K1159201 CDS 1..145 /label=BBa_K1159205 misc_feature 146..151 /label=25/25 scar misc_feature 146..151 /label=BBa_K1159993 misc_feature 158..181 /label=''Strep''-tag II misc_feature 185..205 /label=TEV cleavage site misc_feature 152..208 /label=BBa_K1159309 misc_feature 209..214 /label=25/25 scar misc_feature 209..214 /label=BBa_K1159993 CDS 215..400 /label=Transmembrane Domain of SERK Receptor from ''P. patens'' misc_feature 215..400 /label=BBa_K1159305 misc_feature 401..406 /label=25/25 scar misc_feature 401..406 /label=BBa_K1159993 misc_feature 407..430 /label=MiddleLinker misc_feature 407..430 /label=BBa_K243005 misc_feature 431..436 /label=25/25 scar misc_feature 431..436 /label=BBa_K1159993 CDS 437..1147 /label=GFPmut1 CDS 437..1147 /label=BBa_K1159311 CDS 215..1147 /label=BBa_K1159315 CDS 152..1147 /label=BBa_K1159316 CDS 1..1147 /label=BBa_K1159207 misc_feature 1148..1164 /label=25/10 NC scar misc_feature 1148..1164 /label=BBa_K1159996 RBS 1165..1791 /label=Polioviral IRES RBS 1165..1791 /label=BBa_K1159300 misc_feature 1792..1797 /label=10/25 N-part scar misc_feature 1792..1797 /label=BBa_K1159995 RBS 1798..1801 /label=Consensus Sequence for P. patens CDS 1802..1864 /label=Ig Kappa Signal Peptide misc_feature 1798..1864 /label=BBa_K1159304 misc_feature 1865..1870 /label=25/25 scar misc_feature 1865..1870 /label=BBa_K1159993 CDS 1871..2209 /label=N-terminal FbaB (modified SpyCatcher) misc_feature 1970..1972 /label=Iso --> Glu misc_feature 2075..2077 /label=Met --> Tyr misc_feature 1961..1963 /label=Reactive Lys for isopeptide forming reaction CDS 1871..2209 /label=BBa_K1159200 CDS 1798..2209 /label=BBa_K1159202 misc_feature 2210..2215 /label=25/25 scar misc_feature 2210..2215 /label=BBa_K1159993 CDS 2216..2725 /label=NanoLuc Luciferase CDS 2216..2725 /label=BBa_K1159001 CDS 1798..2725 /label=BBa_K1159211 ORIGIN 1 aacaatgcct ggtgaagttg cttggtggag gcctcttttc cttatcgctc ttatgcctat 61 cggagtgctc tctaacgctg agggtgatgc tcttaacacc accggcgctc atattgtcat 121 ggttgatgct tacaagccaa ctaagaccgg cagcgcttgg tctcatcctc aatttgaaaa 181 gggtgaaaat ctttattttc aaagcggaac cggccctgga caacctcctt ttcctcctcc 241 tcctcctttt acaccacctc ctccacaaac tcctaacggt gcttctggtg agaactctac 301 tggtgctatt gctggtggtg ttgctgctgg tgcagctctt ttgtttgctg ctcctgctat 361 tggattcgct tggtggagaa gaagaaggcc tattgaggct accggcggtg gttctggtgg 421 tggttctggt accggcagta aaggagaaga acttttcact ggagttgtcc caattcttgt 481 tgaattagat ggtgacgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga 541 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc 601 atggccaaca cttgtcacta ctctgactta tggtgttcaa tgcttttcaa gatacccaga 661 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag 721 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg 781 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat 841 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa 901 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt 961 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tcctttcacc 1021 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga 1081 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgagct 1141 ctacaaaacc ggttaatact agagttaaaa cagctctggg gttgtaccca ccccagaggc 1201 ccacgtggcg gctagtactc cggtattgcg gtacccttgt acgcctgttt tatactccct 1261 tcccgtaact tagacgcaca aaaccaagtt caatagaagg gggtacaaac cagtaccacc 1321 acgaacaagc acttctgttt ccccggtgat gtcgtataga ctgcttgcgt ggttgaaagc 1381 gacggatccg ttatccgctt atgtacttcg agaagcccag taccacctcg gaatcttcga 1441 tgcgttgcgc tcagcactca accccagagt gtagcttagg ctgatgagtc tggacatccc 1501 tcaccggtga cggtggtcca ggctgcgttg gcggcctacc tatggctaac gccatgggac 1561 gctagttgtg aacaaggtgt gaagagccta ttgagctaca taagaatcct ccggcccctg 1621 aatgcggcta atcccaacct cggagcaggt ggtcacaaac cagtgattgg cctgtcgtaa 1681 cgcgcaagtc cgtggcggaa ccgactactt tgggtgtccg tgtttccttt tattttattg 1741 tggctgctta tggtgacaat cacagattgt tatcataaag cgaattggat ttactagaac 1801 aatggaaacc gatactctcc tcctctgggt tctcttgctt tgggttccag gatctaccgg 1861 cgataccggc gttgatacct tatcaggttt atcaagtgag caaggtcagt ccggtgatat 1921 gacaattgaa gaagatagtg ctacccatat taaattctca aaacgtgatg aggacggcaa 1981 agagttagct ggtgcaacta tggagttgcg tgattcatct ggtaaaacta ttagtacatg 2041 gatttcagat ggacaagtga aagatttcta cctgtatcca ggaaaatata catttgtcga 2101 aaccgcagca ccagacggtt atgaggtagc aactgctatt acctttacag ttaatgagca 2161 aggtcaggtt actgtaaatg gcaaagcaac taaaggtgac gctcatatta ccggcgtgtt 2221 caccctcgag gatttcgttg gagattggag acagaccgct ggatacaacc ttgatcaggt 2281 tttggagcag ggtggtgtgt ctagtctttt ccagaacctc ggagtgtctg tgacccctat 2341 ccagagaatc gttctctctg gtgagaacgg actcaagatc gatatccacg tgatcatccc 2401 ttacgaggga ctctcaggtg atcagatggg acagatcgag aagattttca aggtggtgta 2461 ccctgttgat gatcaccact tcaaggtgat cctccactac ggaactctcg tgatcgatgg 2521 tgtgacccca aacatgatcg attacttcgg aaggccatac gagggaatcg ctgtgttcga 2581 tggaaagaag attaccgtta ccggaaccct ctggaacgga aacaagatta tcgatgagag 2641 gctcatcaac cctgatggat ctctcttgtt cagggtgacc atcaacggtg tgactggttg 2701 gagactttgc gagagaatcc ttgct //