BBa_K1159215
type: ComponentDefinition
persistent identity: BBa K1159215
display id: BBa_K1159215
version: 1
was generated by: iGEM to SBOL conversion
description: Membrane-anchored SpyTag with secretory SpyCatcher_nLuc reporter fusion translational unit
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part encodes a translational unit consisting two fusion proteins, a Membrane-anchored SpyTag and a secretory SpyCatcher_nLuc (IgKappa-SigP_SpyCatcher_nLuc) reporter fusion to show if post-translational protein fusion like reaction happens between the membrane-localized tag protein and the secretory fusion protein containing N-terminal SpyCatcher and C-terminal NanoLuc Luciferase. As a translational unit both fusion proteins are seperated by a polioviral IRES to allow bicistronic expression in eukaryotic systems. To express this system you only have to insert your favored promoter/terminator in the prefix/suffix of this part. For further information, please see under the subparts linked in the text.
discontinued: false
dominant: false
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_K1159995
component: BBa_K1159300
component: BBa_K1159996
component: BBa_K1159207
component: BBa_K1159211
sequence annotation: BBa_K1159207
sequence annotation: BBa_K1159996
sequence annotation: BBa_K1159300
sequence annotation: BBa_K1159995
sequence annotation: BBa_K1159211
sequence: https://synbiohub.org/public/igem/BBa_K1159215_sequence/1

BBa_K1159995
type: Component
persistent identity: component2350910
display id: component2350910
version: 1
top level: BBa_K1159215
definition: BBa_K1159995
access: public

BBa_K1159300
type: Component
persistent identity: component2350908
display id: component2350908
version: 1
top level: BBa_K1159215
definition: BBa_K1159300
access: public

BBa_K1159996
type: Component
persistent identity: component2350906
display id: component2350906
version: 1
top level: BBa_K1159215
definition: BBa_K1159996
access: public

BBa_K1159207
type: Component
persistent identity: component2350904
display id: component2350904
version: 1
top level: BBa_K1159215
definition: BBa_K1159207
access: public

BBa_K1159211
type: Component
persistent identity: component2350926
display id: component2350926
version: 1
top level: BBa_K1159215
definition: BBa_K1159211
access: public

BBa_K1159207
type: SequenceAnnotation
persistent identity: annotation2350904
display id: annotation2350904
version: 1
top level: BBa_K1159215
location: https://synbiohub.org/public/igem/BBa_K1159215/annotation2350904/range2350904/1
component: BBa_K1159207

https://synbiohub.org/public/igem/BBa_K1159215/annotation2350904/range2350904/1
type: Range
persistent identity: range2350904
display id: range2350904
version: 1
top level: BBa_K1159215
direction: direction/reverse
start: 1
end: 1147
orientation: inline

BBa_K1159996
type: SequenceAnnotation
persistent identity: annotation2350906
display id: annotation2350906
version: 1
top level: BBa_K1159215
location: https://synbiohub.org/public/igem/BBa_K1159215/annotation2350906/range2350906/1
component: BBa_K1159996

https://synbiohub.org/public/igem/BBa_K1159215/annotation2350906/range2350906/1
type: Range
persistent identity: range2350906
display id: range2350906
version: 1
top level: BBa_K1159215
direction: direction/reverse
start: 1148
end: 1164
orientation: inline

BBa_K1159300
type: SequenceAnnotation
persistent identity: annotation2350908
display id: annotation2350908
version: 1
top level: BBa_K1159215
location: https://synbiohub.org/public/igem/BBa_K1159215/annotation2350908/range2350908/1
component: BBa_K1159300

https://synbiohub.org/public/igem/BBa_K1159215/annotation2350908/range2350908/1
type: Range
persistent identity: range2350908
display id: range2350908
version: 1
top level: BBa_K1159215
direction: direction/reverse
start: 1165
end: 1791
orientation: inline

BBa_K1159995
type: SequenceAnnotation
persistent identity: annotation2350910
display id: annotation2350910
version: 1
top level: BBa_K1159215
location: https://synbiohub.org/public/igem/BBa_K1159215/annotation2350910/range2350910/1
component: BBa_K1159995

https://synbiohub.org/public/igem/BBa_K1159215/annotation2350910/range2350910/1
type: Range
persistent identity: range2350910
display id: range2350910
version: 1
top level: BBa_K1159215
direction: direction/reverse
start: 1792
end: 1797
orientation: inline

BBa_K1159211
type: SequenceAnnotation
persistent identity: annotation2350926
display id: annotation2350926
version: 1
top level: BBa_K1159215
location: https://synbiohub.org/public/igem/BBa_K1159215/annotation2350926/range2350926/1
component: BBa_K1159211

https://synbiohub.org/public/igem/BBa_K1159215/annotation2350926/range2350926/1
type: Range
persistent identity: range2350926
display id: range2350926
version: 1
top level: BBa_K1159215
direction: direction/reverse
start: 1798
end: 2725
orientation: inline

BBa_K1159300
type: ComponentDefinition
persistent identity: BBa K1159300
display id: BBa_K1159300
version: 1
was generated by: iGEM to SBOL conversion
description: Polioviral Internal Ribosome Entry Site (IRES) for polycistronic expression in eukaryotes
created: 2013-09-13T11:00:00Z
modified: 2015-06-11T01:55:50Z
mutable provenance: Human Poliovirus 1 Mahoney Strain
owned by: james
owned by: myers
mutable description: This part contains the Internal Ribosome Entry Site (IRES) of the human poliovirus 1 Mahoney strain which allows polycistronic expression systems in eukaryotic systems. This part is flanked by RFC[10] pre- and suffix. By cloning this part betweens CDSs, many proteins/peptides can expressed by a single promoter and terminator.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Polioviral IRES
sequence: https://synbiohub.org/public/igem/BBa_K1159300_sequence/1

Polioviral IRES
type: SequenceAnnotation
persistent identity: annotation2343585
display id: annotation2343585
version: 1
top level: BBa_K1159300
location: https://synbiohub.org/public/igem/BBa_K1159300/annotation2343585/range2343585/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1159300/annotation2343585/range2343585/1
type: Range
persistent identity: range2343585
display id: range2343585
version: 1
top level: BBa_K1159300
direction: direction/forward
start: 1
end: 627
orientation: inline

BBa_K1159995
type: ComponentDefinition
persistent identity: BBa K1159995
display id: BBa_K1159995
version: 1
was generated by: iGEM to SBOL conversion
description: 10/25 N-Part Assembly Scar
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: Assembly scar between RFC[10] coding/non-coding Biobrick and RFC[25] N-part Biobrick (not RFC[25] standard prefix!). Formally it has the same scar like the assembly scar between RFC[10] coding/non-coding Biobrick and RFC[10] coding Biobrick.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: 10/25 N-part scar
sequence: https://synbiohub.org/public/igem/BBa_K1159995_sequence/1

10/25 N-part scar
type: SequenceAnnotation
persistent identity: annotation2342867
display id: annotation2342867
version: 1
top level: BBa_K1159995
location: https://synbiohub.org/public/igem/BBa_K1159995/annotation2342867/range2342867/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1159995/annotation2342867/range2342867/1
type: Range
persistent identity: range2342867
display id: range2342867
version: 1
top level: BBa_K1159995
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_K1159996
type: ComponentDefinition
persistent identity: BBa K1159996
display id: BBa_K1159996
version: 1
was generated by: iGEM to SBOL conversion
description: 25/10 NC Assembly Scar
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: Assembly scar between RFC[25] Biobrick and RFC[10] non-coding Biobrick.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: 25/10 NC scar
sequence: https://synbiohub.org/public/igem/BBa_K1159996_sequence/1

25/10 NC scar
type: SequenceAnnotation
persistent identity: annotation2342869
display id: annotation2342869
version: 1
top level: BBa_K1159996
location: https://synbiohub.org/public/igem/BBa_K1159996/annotation2342869/range2342869/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1159996/annotation2342869/range2342869/1
type: Range
persistent identity: range2342869
display id: range2342869
version: 1
top level: BBa_K1159996
direction: direction/forward
start: 1
end: 17
orientation: inline

BBa_K1159207
type: ComponentDefinition
persistent identity: BBa K1159207
display id: BBa_K1159207
version: 1
was generated by: iGEM to SBOL conversion
description: Membrane-anchored SpyTag in RFC[25] N-Part
created: 2013-09-16T11:00:00Z
modified: 2015-06-11T01:58:39Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part codes for a membrane-anchored version of SpyTag [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159201 BBa_K1159201] for the chassis ''Physcomitrella patens''. Please see subparts for for further description. This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
discontinued: false
dominant: false
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1159205
component: BBa_K1159316
component: BBa_K1159993
sequence annotation: BBa_K1159205
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159316
sequence: https://synbiohub.org/public/igem/BBa_K1159207_sequence/1

BBa_K1159205
type: Component
persistent identity: component2348984
display id: component2348984
version: 1
top level: BBa_K1159207
definition: BBa_K1159205
access: public

BBa_K1159316
type: Component
persistent identity: component2349003
display id: component2349003
version: 1
top level: BBa_K1159207
definition: BBa_K1159316
access: public

BBa_K1159993
type: Component
persistent identity: component2348986
display id: component2348986
version: 1
top level: BBa_K1159207
definition: BBa_K1159993
access: public

BBa_K1159205
type: SequenceAnnotation
persistent identity: annotation2348984
display id: annotation2348984
version: 1
top level: BBa_K1159207
location: https://synbiohub.org/public/igem/BBa_K1159207/annotation2348984/range2348984/1
component: BBa_K1159205

https://synbiohub.org/public/igem/BBa_K1159207/annotation2348984/range2348984/1
type: Range
persistent identity: range2348984
display id: range2348984
version: 1
top level: BBa_K1159207
direction: direction/reverse
start: 1
end: 145
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2348986
display id: annotation2348986
version: 1
top level: BBa_K1159207
location: https://synbiohub.org/public/igem/BBa_K1159207/annotation2348986/range2348986/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159207/annotation2348986/range2348986/1
type: Range
persistent identity: range2348986
display id: range2348986
version: 1
top level: BBa_K1159207
direction: direction/reverse
start: 146
end: 151
orientation: inline

BBa_K1159316
type: SequenceAnnotation
persistent identity: annotation2349003
display id: annotation2349003
version: 1
top level: BBa_K1159207
location: https://synbiohub.org/public/igem/BBa_K1159207/annotation2349003/range2349003/1
component: BBa_K1159316

https://synbiohub.org/public/igem/BBa_K1159207/annotation2349003/range2349003/1
type: Range
persistent identity: range2349003
display id: range2349003
version: 1
top level: BBa_K1159207
direction: direction/reverse
start: 152
end: 1147
orientation: inline

BBa_K1159211
type: ComponentDefinition
persistent identity: BBa K1159211
display id: BBa_K1159211
version: 1
was generated by: iGEM to SBOL conversion
description: Secretory SpyCatcher_nLuc fusion (IgKappa-SigP_SpyCatcher_nLuc) in RFC[25] N-Part
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: Secretory Fusionprotein consisting N-terminal SpyCatcher and C-terminal nLuc (IgKappa-SigP_SpyCatcher_nLuc) in RFC[25] N-Part. This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
discontinued: false
dominant: false
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_K1159001
component: BBa_K1159993
component: BBa_K1159202
sequence annotation: BBa_K1159202
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159001
sequence: https://synbiohub.org/public/igem/BBa_K1159211_sequence/1

BBa_K1159001
type: Component
persistent identity: component2350316
display id: component2350316
version: 1
top level: BBa_K1159211
definition: BBa_K1159001
access: public

BBa_K1159993
type: Component
persistent identity: component2350314
display id: component2350314
version: 1
top level: BBa_K1159211
definition: BBa_K1159993
access: public

BBa_K1159202
type: Component
persistent identity: component2350312
display id: component2350312
version: 1
top level: BBa_K1159211
definition: BBa_K1159202
access: public

BBa_K1159202
type: SequenceAnnotation
persistent identity: annotation2350312
display id: annotation2350312
version: 1
top level: BBa_K1159211
location: https://synbiohub.org/public/igem/BBa_K1159211/annotation2350312/range2350312/1
component: BBa_K1159202

https://synbiohub.org/public/igem/BBa_K1159211/annotation2350312/range2350312/1
type: Range
persistent identity: range2350312
display id: range2350312
version: 1
top level: BBa_K1159211
direction: direction/reverse
start: 1
end: 412
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2350314
display id: annotation2350314
version: 1
top level: BBa_K1159211
location: https://synbiohub.org/public/igem/BBa_K1159211/annotation2350314/range2350314/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159211/annotation2350314/range2350314/1
type: Range
persistent identity: range2350314
display id: range2350314
version: 1
top level: BBa_K1159211
direction: direction/reverse
start: 413
end: 418
orientation: inline

BBa_K1159001
type: SequenceAnnotation
persistent identity: annotation2350316
display id: annotation2350316
version: 1
top level: BBa_K1159211
location: https://synbiohub.org/public/igem/BBa_K1159211/annotation2350316/range2350316/1
component: BBa_K1159001

https://synbiohub.org/public/igem/BBa_K1159211/annotation2350316/range2350316/1
type: Range
persistent identity: range2350316
display id: range2350316
version: 1
top level: BBa_K1159211
direction: direction/reverse
start: 419
end: 928
orientation: inline

BBa_K1159001
type: ComponentDefinition
persistent identity: BBa K1159001
display id: BBa_K1159001
version: 1
was generated by: iGEM to SBOL conversion
description: NanoLuc Luciferase in RFC[25]
created: 2013-05-16T11:00:00Z
modified: 2016-01-26T02:12:53Z
mutable provenance: x
owned by: james
owned by: myers
mutable description: x
discontinued: false
dominant: false
experience: experience/Works
group u list: _1471_
m user id: 4206
owner id: 14732
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: NanoLuc Luciferase
sequence: https://synbiohub.org/public/igem/BBa_K1159001_sequence/1

NanoLuc Luciferase
type: SequenceAnnotation
persistent identity: annotation2342942
display id: annotation2342942
version: 1
top level: BBa_K1159001
location: https://synbiohub.org/public/igem/BBa_K1159001/annotation2342942/range2342942/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1159001/annotation2342942/range2342942/1
type: Range
persistent identity: range2342942
display id: range2342942
version: 1
top level: BBa_K1159001
direction: direction/forward
start: 1
end: 510
orientation: inline

BBa_K1159993
type: ComponentDefinition
persistent identity: BBa K1159993
display id: BBa_K1159993
version: 1
was generated by: iGEM to SBOL conversion
description: 25/25 Assembly Scar
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: Assembly scar between RFC[25] Biobrick and RFC[25] Biobrick.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: 25/25 scar
sequence: https://synbiohub.org/public/igem/BBa_K1159993_sequence/1

25/25 scar
type: SequenceAnnotation
persistent identity: annotation2342863
display id: annotation2342863
version: 1
top level: BBa_K1159993
location: https://synbiohub.org/public/igem/BBa_K1159993/annotation2342863/range2342863/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1159993/annotation2342863/range2342863/1
type: Range
persistent identity: range2342863
display id: range2342863
version: 1
top level: BBa_K1159993
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_K1159316
type: ComponentDefinition
persistent identity: BBa K1159316
display id: BBa_K1159316
version: 1
was generated by: iGEM to SBOL conversion
description: SERK-Receptor TMD with C-terminal GFP and N-terminal Strep-tag II TEV-site linker in RFC[25]
created: 2013-09-15T11:00:00Z
modified: 2015-06-11T01:53:10Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part codes for the Transmembrane Domain of SERK Receptor from ''Physcomitrella patens'' with a C-terminal (intracellular) GFPmut1 fusion and a N-terminal fusion to a linker containing ''Strep''-tag II and a TEV cleavage site; SERK TMD and GFPmut1 are seperated by a middle long linker. This part is flanked by RFC[25] pre- and suffix for further protein fusions. Note: A signal peptide is needed for membrane integration.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_K1159315
component: BBa_K1159993
component: BBa_K1159309
sequence annotation: BBa_K1159309
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159315
sequence: https://synbiohub.org/public/igem/BBa_K1159316_sequence/1

BBa_K1159315
type: Component
persistent identity: component2346363
display id: component2346363
version: 1
top level: BBa_K1159316
definition: BBa_K1159315
access: public

BBa_K1159993
type: Component
persistent identity: component2346352
display id: component2346352
version: 1
top level: BBa_K1159316
definition: BBa_K1159993
access: public

BBa_K1159309
type: Component
persistent identity: component2346350
display id: component2346350
version: 1
top level: BBa_K1159316
definition: BBa_K1159309
access: public

BBa_K1159309
type: SequenceAnnotation
persistent identity: annotation2346350
display id: annotation2346350
version: 1
top level: BBa_K1159316
location: https://synbiohub.org/public/igem/BBa_K1159316/annotation2346350/range2346350/1
component: BBa_K1159309

https://synbiohub.org/public/igem/BBa_K1159316/annotation2346350/range2346350/1
type: Range
persistent identity: range2346350
display id: range2346350
version: 1
top level: BBa_K1159316
direction: direction/reverse
start: 1
end: 57
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2346352
display id: annotation2346352
version: 1
top level: BBa_K1159316
location: https://synbiohub.org/public/igem/BBa_K1159316/annotation2346352/range2346352/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159316/annotation2346352/range2346352/1
type: Range
persistent identity: range2346352
display id: range2346352
version: 1
top level: BBa_K1159316
direction: direction/reverse
start: 58
end: 63
orientation: inline

BBa_K1159315
type: SequenceAnnotation
persistent identity: annotation2346363
display id: annotation2346363
version: 1
top level: BBa_K1159316
location: https://synbiohub.org/public/igem/BBa_K1159316/annotation2346363/range2346363/1
component: BBa_K1159315

https://synbiohub.org/public/igem/BBa_K1159316/annotation2346363/range2346363/1
type: Range
persistent identity: range2346363
display id: range2346363
version: 1
top level: BBa_K1159316
direction: direction/reverse
start: 64
end: 996
orientation: inline

BBa_K1159202
type: ComponentDefinition
persistent identity: BBa K1159202
display id: BBa_K1159202
version: 1
was generated by: iGEM to SBOL conversion
description: Secretory SpyCatcher (IgKappa-SigP_SpyCatcher) in RFC[25] N-Part
created: 2013-09-15T11:00:00Z
modified: 2015-06-11T02:00:29Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part encodes the SpyCatcher [http://parts.igem.org/Part:BBa_K1159200 BBa_K1159200] with a N-terminal signal peptide for secretion (Ig Kappa signal peptide from ''Mus musculus'') which also works in moss ''Physcomitrella patens''. This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
discontinued: false
dominant: false
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_K1159304
component: BBa_K1159200
component: BBa_K1159993
sequence annotation: BBa_K1159304
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159200
sequence: https://synbiohub.org/public/igem/BBa_K1159202_sequence/1

BBa_K1159304
type: Component
persistent identity: component2348476
display id: component2348476
version: 1
top level: BBa_K1159202
definition: BBa_K1159304
access: public

BBa_K1159200
type: Component
persistent identity: component2348483
display id: component2348483
version: 1
top level: BBa_K1159202
definition: BBa_K1159200
access: public

BBa_K1159993
type: Component
persistent identity: component2348478
display id: component2348478
version: 1
top level: BBa_K1159202
definition: BBa_K1159993
access: public

BBa_K1159304
type: SequenceAnnotation
persistent identity: annotation2348476
display id: annotation2348476
version: 1
top level: BBa_K1159202
location: https://synbiohub.org/public/igem/BBa_K1159202/annotation2348476/range2348476/1
component: BBa_K1159304

https://synbiohub.org/public/igem/BBa_K1159202/annotation2348476/range2348476/1
type: Range
persistent identity: range2348476
display id: range2348476
version: 1
top level: BBa_K1159202
direction: direction/reverse
start: 1
end: 67
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2348478
display id: annotation2348478
version: 1
top level: BBa_K1159202
location: https://synbiohub.org/public/igem/BBa_K1159202/annotation2348478/range2348478/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159202/annotation2348478/range2348478/1
type: Range
persistent identity: range2348478
display id: range2348478
version: 1
top level: BBa_K1159202
direction: direction/reverse
start: 68
end: 73
orientation: inline

BBa_K1159200
type: SequenceAnnotation
persistent identity: annotation2348483
display id: annotation2348483
version: 1
top level: BBa_K1159202
location: https://synbiohub.org/public/igem/BBa_K1159202/annotation2348483/range2348483/1
component: BBa_K1159200

https://synbiohub.org/public/igem/BBa_K1159202/annotation2348483/range2348483/1
type: Range
persistent identity: range2348483
display id: range2348483
version: 1
top level: BBa_K1159202
direction: direction/reverse
start: 74
end: 412
orientation: inline

BBa_K1159205
type: ComponentDefinition
persistent identity: BBa K1159205
display id: BBa_K1159205
version: 1
was generated by: iGEM to SBOL conversion
description: Secretory SpyTag (SERK-SigP_SpyTag) in RFC[25] N-Part
created: 2013-09-16T11:00:00Z
modified: 2015-06-11T01:59:37Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part encodes the SpyTag [http://parts.igem.org/Part:BBa_K1159201 BBa_K1159201] with a N-terminal signal peptide for secretion (SERK receptor signal peptide from ''Physcomitrella patens''). This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
discontinued: false
dominant: false
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_K1159993
component: BBa_K1159303
component: BBa_K1159201
sequence annotation: BBa_K1159303
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159201
sequence: https://synbiohub.org/public/igem/BBa_K1159205_sequence/1

BBa_K1159993
type: Component
persistent identity: component2348900
display id: component2348900
version: 1
top level: BBa_K1159205
definition: BBa_K1159993
access: public

BBa_K1159303
type: Component
persistent identity: component2348898
display id: component2348898
version: 1
top level: BBa_K1159205
definition: BBa_K1159303
access: public

BBa_K1159201
type: Component
persistent identity: component2348903
display id: component2348903
version: 1
top level: BBa_K1159205
definition: BBa_K1159201
access: public

BBa_K1159303
type: SequenceAnnotation
persistent identity: annotation2348898
display id: annotation2348898
version: 1
top level: BBa_K1159205
location: https://synbiohub.org/public/igem/BBa_K1159205/annotation2348898/range2348898/1
component: BBa_K1159303

https://synbiohub.org/public/igem/BBa_K1159205/annotation2348898/range2348898/1
type: Range
persistent identity: range2348898
display id: range2348898
version: 1
top level: BBa_K1159205
direction: direction/reverse
start: 1
end: 100
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2348900
display id: annotation2348900
version: 1
top level: BBa_K1159205
location: https://synbiohub.org/public/igem/BBa_K1159205/annotation2348900/range2348900/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159205/annotation2348900/range2348900/1
type: Range
persistent identity: range2348900
display id: range2348900
version: 1
top level: BBa_K1159205
direction: direction/reverse
start: 101
end: 106
orientation: inline

BBa_K1159201
type: SequenceAnnotation
persistent identity: annotation2348903
display id: annotation2348903
version: 1
top level: BBa_K1159205
location: https://synbiohub.org/public/igem/BBa_K1159205/annotation2348903/range2348903/1
component: BBa_K1159201

https://synbiohub.org/public/igem/BBa_K1159205/annotation2348903/range2348903/1
type: Range
persistent identity: range2348903
display id: range2348903
version: 1
top level: BBa_K1159205
direction: direction/reverse
start: 107
end: 145
orientation: inline

BBa_K1159303
type: ComponentDefinition
persistent identity: BBa K1159303
display id: BBa_K1159303
version: 1
was generated by: iGEM to SBOL conversion
description: Signal Peptide of SERK Receptor from Physcomitrella patens in RFC[25]
created: 2013-09-13T11:00:00Z
modified: 2015-06-11T01:54:49Z
mutable provenance: ''Physcomitrella patens''
owned by: james
owned by: myers
mutable description: This part codes for the signal peptide from the SERK receptor (Somatic Embryogenesis RECEPTOR KINAS) of ''Physocmitrella patens''. The signal peptide induces translocation of the protein into the Endoplasmatic Reticulum (ER) for secretion or membrane integration. Protein fused to the C-terminus of this part should be secreted or otherwise if the part fused region contains a transmembrane domain it will be integrated in cytoplasmatic membrane. This part starts with a consensus sequence for ''Physcomitrella patens'' which allows efficient translation start. Furthermore, this part is flanked by RFC[25] N-part pre- and suffix for fusions to the C-terminus of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence annotation: Consensus Sequence for ''P. patens''
sequence annotation: SERK Signal Peptide
sequence: https://synbiohub.org/public/igem/BBa_K1159303_sequence/1

Consensus Sequence for ''P. patens''
type: SequenceAnnotation
persistent identity: annotation2341512
display id: annotation2341512
version: 1
top level: BBa_K1159303
location: https://synbiohub.org/public/igem/BBa_K1159303/annotation2341512/range2341512/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1159303/annotation2341512/range2341512/1
type: Range
persistent identity: range2341512
display id: range2341512
version: 1
top level: BBa_K1159303
direction: direction/forward
start: 1
end: 4
orientation: inline

SERK Signal Peptide
type: SequenceAnnotation
persistent identity: annotation2341513
display id: annotation2341513
version: 1
top level: BBa_K1159303
location: https://synbiohub.org/public/igem/BBa_K1159303/annotation2341513/range2341513/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1159303/annotation2341513/range2341513/1
type: Range
persistent identity: range2341513
display id: range2341513
version: 1
top level: BBa_K1159303
direction: direction/forward
start: 5
end: 100
orientation: inline

BBa_K1159309
type: ComponentDefinition
persistent identity: BBa K1159309
display id: BBa_K1159309
version: 1
was generated by: iGEM to SBOL conversion
description: 19 AA Linker with N-terminal Strep-tag II and C-terminal TEV cleavage site in RFC[25]
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part encodes a 19 amino acids long linker with a N-terminal ''Strep''-tag II and a C-terminal cleavage site for the TEV Protease. For further protein fusions this part is flanked by RFC[25} pre- and suffix.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence annotation: ''Strep''-tag II
sequence annotation: TEV cleavage site
sequence: https://synbiohub.org/public/igem/BBa_K1159309_sequence/1

''Strep''-tag II
type: SequenceAnnotation
persistent identity: annotation2342578
display id: annotation2342578
version: 1
top level: BBa_K1159309
location: https://synbiohub.org/public/igem/BBa_K1159309/annotation2342578/range2342578/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K1159309/annotation2342578/range2342578/1
type: Range
persistent identity: range2342578
display id: range2342578
version: 1
top level: BBa_K1159309
direction: direction/forward
start: 7
end: 30
orientation: inline

TEV cleavage site
type: SequenceAnnotation
persistent identity: annotation2342591
display id: annotation2342591
version: 1
top level: BBa_K1159309
location: https://synbiohub.org/public/igem/BBa_K1159309/annotation2342591/range2342591/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1159309/annotation2342591/range2342591/1
type: Range
persistent identity: range2342591
display id: range2342591
version: 1
top level: BBa_K1159309
direction: direction/forward
start: 34
end: 54
orientation: inline

BBa_K1159315
type: ComponentDefinition
persistent identity: BBa K1159315
display id: BBa_K1159315
version: 1
was generated by: iGEM to SBOL conversion
description: TMD of SERK Receptor from Physcomitrella patens with C-terminal GFP fusion in RFC[25]
created: 2013-09-15T11:00:00Z
modified: 2015-05-28T03:06:34Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part codes for the Transmembrane Domain of SERK Receptor from Physcomitrella patens'' with a C-terminal (intracellular) GFPmut1 fusion; SERK TMD and GFPmut1 are seperated by a middle long linker. Still, a signal peptide is needed for membrane integration. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_K1159993
component: BBa_K243005
component: BBa_K1159305
component: BBa_K1159311
sequence annotation: BBa_K1159305
sequence annotation: BBa_K1159993
sequence annotation: BBa_K243005
sequence annotation: BBa_K1159311
sequence: https://synbiohub.org/public/igem/BBa_K1159315_sequence/1

BBa_K1159993
type: Component
persistent identity: component2346340
display id: component2346340
version: 1
top level: BBa_K1159315
definition: BBa_K1159993
access: public

BBa_K243005
type: Component
persistent identity: component2346338
display id: component2346338
version: 1
top level: BBa_K1159315
definition: MiddleLink
access: public

BBa_K1159993
type: Component
persistent identity: component2346336
display id: component2346336
version: 1
top level: BBa_K1159315
definition: BBa_K1159993
access: public

BBa_K1159305
type: Component
persistent identity: component2346334
display id: component2346334
version: 1
top level: BBa_K1159315
definition: BBa_K1159305
access: public

BBa_K1159311
type: Component
persistent identity: component2346342
display id: component2346342
version: 1
top level: BBa_K1159315
definition: BBa_K1159311
access: public

BBa_K1159305
type: SequenceAnnotation
persistent identity: annotation2346334
display id: annotation2346334
version: 1
top level: BBa_K1159315
location: https://synbiohub.org/public/igem/BBa_K1159315/annotation2346334/range2346334/1
component: BBa_K1159305

https://synbiohub.org/public/igem/BBa_K1159315/annotation2346334/range2346334/1
type: Range
persistent identity: range2346334
display id: range2346334
version: 1
top level: BBa_K1159315
direction: direction/reverse
start: 1
end: 186
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2346336
display id: annotation2346336
version: 1
top level: BBa_K1159315
location: https://synbiohub.org/public/igem/BBa_K1159315/annotation2346336/range2346336/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159315/annotation2346336/range2346336/1
type: Range
persistent identity: range2346336
display id: range2346336
version: 1
top level: BBa_K1159315
direction: direction/reverse
start: 187
end: 192
orientation: inline

BBa_K243005
type: SequenceAnnotation
persistent identity: annotation2346338
display id: annotation2346338
version: 1
top level: BBa_K1159315
location: https://synbiohub.org/public/igem/BBa_K1159315/annotation2346338/range2346338/1
component: BBa_K243005

https://synbiohub.org/public/igem/BBa_K1159315/annotation2346338/range2346338/1
type: Range
persistent identity: range2346338
display id: range2346338
version: 1
top level: BBa_K1159315
direction: direction/reverse
start: 193
end: 216
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2346340
display id: annotation2346340
version: 1
top level: BBa_K1159315
location: https://synbiohub.org/public/igem/BBa_K1159315/annotation2346340/range2346340/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159315/annotation2346340/range2346340/1
type: Range
persistent identity: range2346340
display id: range2346340
version: 1
top level: BBa_K1159315
direction: direction/reverse
start: 217
end: 222
orientation: inline

BBa_K1159311
type: SequenceAnnotation
persistent identity: annotation2346342
display id: annotation2346342
version: 1
top level: BBa_K1159315
location: https://synbiohub.org/public/igem/BBa_K1159315/annotation2346342/range2346342/1
component: BBa_K1159311

https://synbiohub.org/public/igem/BBa_K1159315/annotation2346342/range2346342/1
type: Range
persistent identity: range2346342
display id: range2346342
version: 1
top level: BBa_K1159315
direction: direction/reverse
start: 223
end: 933
orientation: inline

BBa_K1159304
type: ComponentDefinition
persistent identity: BBa K1159304
display id: BBa_K1159304
version: 1
was generated by: iGEM to SBOL conversion
description: Signal Peptide of Ig Kappa chain from Mus musculus in RFC[25] N-part
created: 2013-09-13T11:00:00Z
modified: 2015-06-11T01:54:39Z
mutable provenance: ''Mus musculus''
owned by: james
owned by: myers
mutable description: This part codes for the signal peptide of the Ig Kappa chain from ''Mus musculus''. The signal peptide induces translocation of the protein into the Endoplasmatic Reticulum (ER) for secretion or membrane integration. Protein fused to the C-terminus of this part should be secreted or otherwise if the part fused region contains a transmembrane domain it will be integrated in cytoplasmatic membrane. This part starts with a consensus sequence for Physcomitrella patens which allows efficient translation start. Furthermore, this part is flanked by RFC[25] N-part pre- and suffix for fusions to the C-terminus of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence annotation: Consensus Sequence for P. patens
sequence annotation: Ig Kappa Signal Peptide
sequence: https://synbiohub.org/public/igem/BBa_K1159304_sequence/1

Consensus Sequence for P. patens
type: SequenceAnnotation
persistent identity: annotation2341498
display id: annotation2341498
version: 1
top level: BBa_K1159304
location: https://synbiohub.org/public/igem/BBa_K1159304/annotation2341498/range2341498/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1159304/annotation2341498/range2341498/1
type: Range
persistent identity: range2341498
display id: range2341498
version: 1
top level: BBa_K1159304
direction: direction/forward
start: 1
end: 4
orientation: inline

Ig Kappa Signal Peptide
type: SequenceAnnotation
persistent identity: annotation2341499
display id: annotation2341499
version: 1
top level: BBa_K1159304
location: https://synbiohub.org/public/igem/BBa_K1159304/annotation2341499/range2341499/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1159304/annotation2341499/range2341499/1
type: Range
persistent identity: range2341499
display id: range2341499
version: 1
top level: BBa_K1159304
direction: direction/forward
start: 5
end: 67
orientation: inline

BBa_K1159200
type: ComponentDefinition
persistent identity: BBa K1159200
display id: BBa_K1159200
version: 1
was generated by: iGEM to SBOL conversion
description: Splitted and engineered N-terminal FbaB for isopeptide bound formation (SpyCatcher) in RFC[25]
created: 2013-09-13T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: ''Streptococcus pyogenes''
owned by: james
owned by: myers
mutable description: This part codes for a protein that recognizes and forms a covalent isopeptide bound to a oligopeptide. That oligopeptide can be found under BBa_K1159201. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: N-terminal FbaB (modified SpyCatcher)
sequence annotation: Reactive Lys for isopeptide forming reaction
sequence annotation: Iso --> Glu
sequence annotation: Met --> Tyr
sequence: https://synbiohub.org/public/igem/BBa_K1159200_sequence/1

N-terminal FbaB (modified SpyCatcher)
type: SequenceAnnotation
persistent identity: annotation2343345
display id: annotation2343345
version: 1
top level: BBa_K1159200
location: https://synbiohub.org/public/igem/BBa_K1159200/annotation2343345/range2343345/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1159200/annotation2343345/range2343345/1
type: Range
persistent identity: range2343345
display id: range2343345
version: 1
top level: BBa_K1159200
direction: direction/forward
start: 1
end: 339
orientation: inline

Reactive Lys for isopeptide forming reaction
type: SequenceAnnotation
persistent identity: annotation2343366
display id: annotation2343366
version: 1
top level: BBa_K1159200
location: https://synbiohub.org/public/igem/BBa_K1159200/annotation2343366/range2343366/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_K1159200/annotation2343366/range2343366/1
type: Range
persistent identity: range2343366
display id: range2343366
version: 1
top level: BBa_K1159200
direction: direction/forward
start: 91
end: 93
orientation: inline

Iso --> Glu
type: SequenceAnnotation
persistent identity: annotation2343348
display id: annotation2343348
version: 1
top level: BBa_K1159200
location: https://synbiohub.org/public/igem/BBa_K1159200/annotation2343348/range2343348/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K1159200/annotation2343348/range2343348/1
type: Range
persistent identity: range2343348
display id: range2343348
version: 1
top level: BBa_K1159200
direction: direction/forward
start: 100
end: 102
orientation: inline

Met --> Tyr
type: SequenceAnnotation
persistent identity: annotation2343349
display id: annotation2343349
version: 1
top level: BBa_K1159200
location: https://synbiohub.org/public/igem/BBa_K1159200/annotation2343349/range2343349/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K1159200/annotation2343349/range2343349/1
type: Range
persistent identity: range2343349
display id: range2343349
version: 1
top level: BBa_K1159200
direction: direction/forward
start: 205
end: 207
orientation: inline

BBa_K1159305
type: ComponentDefinition
persistent identity: BBa K1159305
display id: BBa_K1159305
version: 1
was generated by: iGEM to SBOL conversion
description: Transmembrane domain of SERK Receptor from Physcomitrella patens in RFC[25]
created: 2013-09-13T11:00:00Z
modified: 2015-06-11T01:54:11Z
mutable provenance: ''Physcomitrella patens''
owned by: james
owned by: myers
mutable description: This part is encoding the transmembrane region of the SERK Receptor from ''Physcomitrella patens''. For membrane translocation a N-terminal signal peptide is still necessary for translocation through the ER membrane. This part is flanked by RFC[25] pre- and suffix.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Transmembrane Domain of SERK Receptor from ''P. patens''
sequence: https://synbiohub.org/public/igem/BBa_K1159305_sequence/1

Transmembrane Domain of SERK Receptor from ''P. patens''
type: SequenceAnnotation
persistent identity: annotation2343588
display id: annotation2343588
version: 1
top level: BBa_K1159305
location: https://synbiohub.org/public/igem/BBa_K1159305/annotation2343588/range2343588/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1159305/annotation2343588/range2343588/1
type: Range
persistent identity: range2343588
display id: range2343588
version: 1
top level: BBa_K1159305
direction: direction/forward
start: 1
end: 186
orientation: inline

BBa_K1159201
type: ComponentDefinition
persistent identity: BBa K1159201
display id: BBa_K1159201
version: 1
was generated by: iGEM to SBOL conversion
description: Splitted and engineered C-terminal FbaB for isopeptide bound formation (SpyTag) in RFC[25]
created: 2013-09-13T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: ''Streptococcus pyogenes''
owned by: james
owned by: myers
mutable description: This part codes for a oligopeptide that is recognized and bound by a covalent isopeptide bound to a protein, called SpyCatcher. That catcher protein for this oligopeptdie can be found under BBa_K1159200. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: SpyTag
sequence annotation: Reactive Asp for isopeptide forming reaction
sequence: https://synbiohub.org/public/igem/BBa_K1159201_sequence/1

SpyTag
type: SequenceAnnotation
persistent identity: annotation2343481
display id: annotation2343481
version: 1
top level: BBa_K1159201
location: https://synbiohub.org/public/igem/BBa_K1159201/annotation2343481/range2343481/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K1159201/annotation2343481/range2343481/1
type: Range
persistent identity: range2343481
display id: range2343481
version: 1
top level: BBa_K1159201
direction: direction/forward
start: 1
end: 39
orientation: inline

Reactive Asp for isopeptide forming reaction
type: SequenceAnnotation
persistent identity: annotation2343512
display id: annotation2343512
version: 1
top level: BBa_K1159201
location: https://synbiohub.org/public/igem/BBa_K1159201/annotation2343512/range2343512/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_K1159201/annotation2343512/range2343512/1
type: Range
persistent identity: range2343512
display id: range2343512
version: 1
top level: BBa_K1159201
direction: direction/forward
start: 19
end: 21
orientation: inline

MiddleLink
type: ComponentDefinition
persistent identity: BBa K243005
display id: BBa_K243005
version: 1
was generated by: iGEM to SBOL conversion
description: Middle Linker ( Gly-Gly-Ser-Gly)x2
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:37Z
mutable provenance: Oligos synthesized by sigma. Hybridized by PCR.
owned by: james
owned by: myers
mutable description: This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. We used this part to connect our protein domains with our lipocalin domains, for our project the universal endonuclease.The sequence produced the aminoacids Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly .
discontinued: false
dominant: false
experience: experience/None
group u list: _352_
m user id: 0
owner id: 4732
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: Freiburg Bioware09
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence annotation: MiddleLinker
sequence: https://synbiohub.org/public/igem/BBa_K243005_sequence/1

MiddleLinker
type: SequenceAnnotation
persistent identity: annotation2041884
display id: annotation2041884
version: 1
top level: MiddleLink
location: https://synbiohub.org/public/igem/BBa_K243005/annotation2041884/range2041884/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K243005/annotation2041884/range2041884/1
type: Range
persistent identity: range2041884
display id: range2041884
version: 1
top level: MiddleLink
direction: direction/forward
start: 1
end: 24
orientation: inline

BBa_K1159311
type: ComponentDefinition
persistent identity: BBa K1159311
display id: BBa_K1159311
version: 1
was generated by: iGEM to SBOL conversion
description: GFPmut1
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: ''Aequarea victoria''
owned by: james
owned by: myers
mutable description: This part codes for GFPmut1 which is flanked by RFC[25] pre- and suffix.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFPmut1
sequence: https://synbiohub.org/public/igem/BBa_K1159311_sequence/1

GFPmut1
type: SequenceAnnotation
persistent identity: annotation2343590
display id: annotation2343590
version: 1
top level: BBa_K1159311
location: https://synbiohub.org/public/igem/BBa_K1159311/annotation2343590/range2343590/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1159311/annotation2343590/range2343590/1
type: Range
persistent identity: range2343590
display id: range2343590
version: 1
top level: BBa_K1159311
direction: direction/forward
start: 1
end: 711
orientation: inline

https://synbiohub.org/public/igem/BBa_K1159215_sequence/1
type: Sequence
persistent identity: BBa K1159215 sequence
display id: BBa_K1159215_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacaccaccggcgctcatattgtcatggttgatgcttacaagccaactaagaccggcagcgcttggtctcatcctcaatttgaaaagggtgaaaatctttattttcaaagcggaaccggccctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggctaccggcggtggttctggtggtggttctggtaccggcagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgagctctacaaaaccggttaatactagagttaaaacagctctggggttgtacccaccccagaggcccacgtggcggctagtactccggtattgcggtacccttgtacgcctgttttatactcccttcccgtaacttagacgcacaaaaccaagttcaatagaagggggtacaaaccagtaccaccacgaacaagcacttctgtttccccggtgatgtcgtatagactgcttgcgtggttgaaagcgacggatccgttatccgcttatgtacttcgagaagcccagtaccacctcggaatcttcgatgcgttgcgctcagcactcaaccccagagtgtagcttaggctgatgagtctggacatccctcaccggtgacggtggtccaggctgcgttggcggcctacctatggctaacgccatgggacgctagttgtgaacaaggtgtgaagagcctattgagctacataagaatcctccggcccctgaatgcggctaatcccaacctcggagcaggtggtcacaaaccagtgattggcctgtcgtaacgcgcaagtccgtggcggaaccgactactttgggtgtccgtgtttccttttattttattgtggctgcttatggtgacaatcacagattgttatcataaagcgaattggatttactagaacaatggaaaccgatactctcctcctctgggttctcttgctttgggttccaggatctaccggcgataccggcgttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaacta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159300_sequence/1
type: Sequence
persistent identity: BBa K1159300 sequence
display id: BBa_K1159300_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaaaacagctctggggttgtacccaccccagaggcccacgtggcggctagtactccggtattgcggtacccttgtacgcctgttttatactcccttcccgtaacttagacgcacaaaaccaagttcaatagaagggggtacaaaccagtaccaccacgaacaagcacttctgtttccccggtgatgtcgtatagactgcttgcgtggttgaaagcgacggatccgttatccgcttatgtacttcgagaagcccagtaccacctcggaatcttcgatgcgttgcgctcagcactcaaccccagagtgtagcttaggctgatgagtctggacatccctcaccggtgacggtggtccaggctgcgttggcggcctacctatggctaacgccatgggacgctagttgtgaacaaggtgtgaagagcctattgagctacataagaatcctccggcccctgaatgcggctaatcccaacctcggagcaggtggtcacaaaccagtgattggcctgtcgtaacgcgcaagtccgtggcggaaccgactactttgggtgtccgtgtttccttttattttattgtggctgcttatggtgacaatcacagattgttatcataaagcgaattggatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159995_sequence/1
type: Sequence
persistent identity: BBa K1159995 sequence
display id: BBa_K1159995_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: tactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159996_sequence/1
type: Sequence
persistent identity: BBa K1159996 sequence
display id: BBa_K1159996_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accggttaatactagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159207_sequence/1
type: Sequence
persistent identity: BBa K1159207 sequence
display id: BBa_K1159207_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacaccaccggcgctcatattgtcatggttgatgcttacaagccaactaagaccggcagcgcttggtctcatcctcaatttgaaaagggtgaaaatctttattttcaaagcggaaccggccctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggctaccggcggtggttctggtggtggttctggtaccggcagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgagctctacaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159211_sequence/1
type: Sequence
persistent identity: BBa K1159211 sequence
display id: BBa_K1159211_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacaatggaaaccgatactctcctcctctgggttctcttgctttgggttccaggatctaccggcgataccggcgttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatattaccggcgtgttcaccctcgaggatttcgttggagattggagacagaccgctggatacaaccttgatcaggttttggagcagggtggtgtgtctagtcttttccagaacctcggagtgtctgtgacccctatccagagaatcgttctctctggtgagaacggactcaagatcgatatccacgtgatcatcccttacgagggactctcaggtgatcagatgggacagatcgagaagattttcaaggtggtgtaccctgttgatgatcaccacttcaaggtgatcctccactacggaactctcgtgatcgatggtgtgaccccaaacatgatcgattacttcggaaggccatacgagggaatcgctgtgttcgatggaaagaagattaccgttaccggaaccctctggaacggaaacaagattatcgatgagaggctcatcaaccctgatggatctctcttgttcagggtgaccatcaacggtgtgactggttggagactttgcgagagaatccttgct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159001_sequence/1
type: Sequence
persistent identity: BBa K1159001 sequence
display id: BBa_K1159001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgttcaccctcgaggatttcgttggagattggagacagaccgctggatacaaccttgatcaggttttggagcagggtggtgtgtctagtcttttccagaacctcggagtgtctgtgacccctatccagagaatcgttctctctggtgagaacggactcaagatcgatatccacgtgatcatcccttacgagggactctcaggtgatcagatgggacagatcgagaagattttcaaggtggtgtaccctgttgatgatcaccacttcaaggtgatcctccactacggaactctcgtgatcgatggtgtgaccccaaacatgatcgattacttcggaaggccatacgagggaatcgctgtgttcgatggaaagaagattaccgttaccggaaccctctggaacggaaacaagattatcgatgagaggctcatcaaccctgatggatctctcttgttcagggtgaccatcaacggtgtgactggttggagactttgcgagagaatccttgct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159993_sequence/1
type: Sequence
persistent identity: BBa K1159993 sequence
display id: BBa_K1159993_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159316_sequence/1
type: Sequence
persistent identity: BBa K1159316 sequence
display id: BBa_K1159316_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agcgcttggtctcatcctcaatttgaaaagggtgaaaatctttattttcaaagcggaaccggccctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggctaccggcggtggttctggtggtggttctggtaccggcagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgagctctacaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159202_sequence/1
type: Sequence
persistent identity: BBa K1159202 sequence
display id: BBa_K1159202_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacaatggaaaccgatactctcctcctctgggttctcttgctttgggttccaggatctaccggcgataccggcgttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159205_sequence/1
type: Sequence
persistent identity: BBa K1159205 sequence
display id: BBa_K1159205_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacaccaccggcgctcatattgtcatggttgatgcttacaagccaactaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159303_sequence/1
type: Sequence
persistent identity: BBa K1159303 sequence
display id: BBa_K1159303_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159309_sequence/1
type: Sequence
persistent identity: BBa K1159309 sequence
display id: BBa_K1159309_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agcgcttggtctcatcctcaatttgaaaagggtgaaaatctttattttcaaagcgga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159315_sequence/1
type: Sequence
persistent identity: BBa K1159315 sequence
display id: BBa_K1159315_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggctaccggcggtggttctggtggtggttctggtaccggcagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgagctctacaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159304_sequence/1
type: Sequence
persistent identity: BBa K1159304 sequence
display id: BBa_K1159304_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacaatggaaaccgatactctcctcctctgggttctcttgctttgggttccaggatctaccggcgat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159200_sequence/1
type: Sequence
persistent identity: BBa K1159200 sequence
display id: BBa_K1159200_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159305_sequence/1
type: Sequence
persistent identity: BBa K1159305 sequence
display id: BBa_K1159305_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159201_sequence/1
type: Sequence
persistent identity: BBa K1159201 sequence
display id: BBa_K1159201_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctcatattgtcatggttgatgcttacaagccaactaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K243005_sequence/1
type: Sequence
persistent identity: BBa K243005 sequence
display id: BBa_K243005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtggttctggtggtggttctggt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159311_sequence/1
type: Sequence
persistent identity: BBa K1159311 sequence
display id: BBa_K1159311_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgagctctacaaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z