LOCUS       BBa_K1159316             996 bp    DNA     linear       25-Nov-2024
DEFINITION  SERK-Receptor TMD with C-terminal GFP and N-terminal 
            <i>Strep</i>-tag II TEV-site linker in RFC[25]
ACCESSION   BBa_K1159316
VERSION     BBa_K1159316.1  
FEATURES             Location/Qualifiers
     misc_feature    7..30
                     /label=''Strep''-tag II
     misc_feature    34..54
                     /label=TEV cleavage site
     misc_feature    1..57
                     /label=BBa_K1159309
     misc_feature    58..63
                     /label=25/25 scar
     misc_feature    58..63
                     /label=BBa_K1159993
     CDS             64..249
                     /label=Transmembrane Domain of SERK Receptor from ''P. 
                     patens''
     misc_feature    64..249
                     /label=BBa_K1159305
     misc_feature    250..255
                     /label=25/25 scar
     misc_feature    250..255
                     /label=BBa_K1159993
     misc_feature    256..279
                     /label=MiddleLinker
     misc_feature    256..279
                     /label=BBa_K243005
     misc_feature    280..285
                     /label=25/25 scar
     misc_feature    280..285
                     /label=BBa_K1159993
     CDS             286..996
                     /label=GFPmut1
     CDS             286..996
                     /label=BBa_K1159311
     CDS             64..996
                     /label=BBa_K1159315
ORIGIN
        1 agcgcttggt ctcatcctca atttgaaaag ggtgaaaatc tttattttca aagcggaacc
       61 ggccctggac aacctccttt tcctcctcct cctcctttta caccacctcc tccacaaact
      121 cctaacggtg cttctggtga gaactctact ggtgctattg ctggtggtgt tgctgctggt
      181 gcagctcttt tgtttgctgc tcctgctatt ggattcgctt ggtggagaag aagaaggcct
      241 attgaggcta ccggcggtgg ttctggtggt ggttctggta ccggcagtaa aggagaagaa
      301 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgacgttaa tgggcacaaa
      361 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
      421 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tctgacttat
      481 ggtgttcaat gcttttcaag atacccagat catatgaaac agcatgactt tttcaagagt
      541 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac
      601 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
      661 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
      721 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa
      781 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
      841 ccaattggcg atggccctgt cctttcacca gacaaccatt acctgtccac acaatctgcc
      901 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
      961 gctgggatta cacatggcat ggatgagctc tacaaa
//