LOCUS       BBa_K1159317            3285 bp    DNA     linear       06-Dec-2025
DEFINITION  pRD29A_Strong-Plant-Kozak-(RBS)_RFP_t35S_nptII-cassette
ACCESSION   BBa_K1159317
VERSION     BBa_K1159317.1  
FEATURES             Location/Qualifiers
     misc_feature    855..857
                     /label=RFP start codon
     RBS             843..854
                     /label=Kozak
     misc_feature    837..842
                     /label=Kpn I
     promoter        1..836
                     /label=RD29A
     CDS             855..1529
                     /label=RFP
     misc_feature    1530..1535
                     /label=Translational Stop 2x
     misc_feature    1..1535
                     /label=BBa_K414000
     misc_feature    1536..1543
                     /label=10/10 NC scar
     misc_feature    1536..1543
                     /label=BBa_K1159991
     misc_feature    1544..1760
                     /label=CaMV 35S Terminator
     terminator      1544..1760
                     /label=BBa_K1159307
     misc_feature    1761..1768
                     /label=10/10 NC scar
     misc_feature    1761..1768
                     /label=BBa_K1159991
     promoter        1823..2129
                     /label=NOS Promoter
     CDS             2142..2936
                     /label=Neomycin phosphotransferase II
     misc_feature    3012..3267
                     /label=NOS Terminator
     misc_feature    1769..3285
                     /label=BBa_K1159308
ORIGIN
        1 aggtagaact tatatacatt atattgtaat tttttgtaac aaaatgtttt tattattatt
       61 atagaatttt actggttaaa ttaaaaatga atagaaaagg tgaattaaga ggagagagga
      121 ggtaaacatt ttcttctatt ttttcatatt ttcaggataa attattgtaa aagtttacaa
      181 gatttccatt tgactagggt aaatgaggaa tattctctag taagatcatt atttcatcta
      241 cttcttttat cttctaccag tagaggaata aacaatattt agctcctttg taaatacaaa
      301 ttaattttcc ttcttgacat cattcaattt taattttacg tataaaataa aagatcatac
      361 ctattagaac gattaaggag aaatacaatt cgaatgagaa ggatgtgccg tttgttataa
      421 taaacagcca cacgacgtaa acgtaaaatg accacatgat gggccaatag acatggaccg
      481 actactaata atagtaagtt acattttagg atggaataaa tatcataccg acatcagttt
      541 tgaaagaaaa gggaaaaaaa gaaaaaataa ataaaagata tactaccgac atgagttcca
      601 aaaagcaaaa aaaaagatca agccgacaca gacacgcgta gagagcaaaa tgactttgac
      661 gtcacaccac gaaaacagac gcttcatacg tgtcccttta tctctctcag tctctctata
      721 aacttagtga gaccctcctc tgttttactc acaaatatgc aaactagaaa acaatcatca
      781 ggaataaagg gtttgattac ttctattgga aagaaaaaaa tctttggaaa atggatggta
      841 ccaaaaaaaa aacaatggcc tcctccgagg acgtcatcaa ggagttcatg cgcttcaagg
      901 tgcgcatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
      961 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct
     1021 tcgcctggga catcctgtcc cctcagttcc agtacggctc caaggcctac gtgaagcacc
     1081 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
     1141 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctccaggacg
     1201 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     1261 tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg gatgtacccc gaggacggcg
     1321 ccctgaaggg cgagatcaag atgaggctga agctgaagga cggcggccac tacgacgccg
     1381 aggtcaagac cacctacatg gccaagaagc ccgtgcagct gcccggcgcc tacaagaccg
     1441 acatcaagct ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc
     1501 gcgccgaggg ccgccactcc accggcgcct aataatacta gagctagagt ccgcaaaaat
     1561 caccagtctc tctctacaaa tctatctctc tctatttttc tccagaataa tgtgtgagta
     1621 gttcccagat aagggaatta gggttcttat agggtttcgc tcatgtgttg agcatataag
     1681 aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct
     1741 aaaaccaaaa tccagtgacc tactagagat cggatcctgt caaacactga tagtttaaac
     1801 tgaaggcggg aaacgacaat ctgatcatga gcggagaatt aagggagtca cgttatgacc
     1861 cccgccgatg acgcgggaca agccgtttta cgtttggaac tgacagaacc gcaacgttga
     1921 aggagccact cagccgcggg tttctggagt ttaatgagct aagcacatac gtcagaaacc
     1981 attattgcgc gttcaaaagt cgcctaaggt cactatcagc tagcaaatat ttcttgtcaa
     2041 aaatgctcca ctgacgttcc ataaattccc ctcggtatcc aattagagtc tcatattcac
     2101 tctcaatcca aataatctgc accggatctg gatcgtttcg catgattgaa caagatggat
     2161 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     2221 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     2281 tttttgtcaa gaccgacctg tccggtgccc tgaatgaatt gcaggacgag gcagcgcggc
     2341 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     2401 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     2461 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     2521 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     2581 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     2641 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     2701 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     2761 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     2821 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     2881 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     2941 gacccaagct ttagatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc
     3001 ggatgatccc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     3061 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     3121 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     3181 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     3241 cggtgtcatc tatgttacta gatcgggcct cctgtcaagc ttgat
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