##gff-version 3
##sequence-region BBa_K1159317 1 3285
BBa_K1159317	.	sequence_feature	1761	1768	.	+	0	ID=BBa_K1159991;Name=BBa_K1159991
BBa_K1159317	.	sequence_feature	1761	1768	.	+	0	ID=annotation2342822;Name=10/10 NC scar;Parent=BBa_K1159991
BBa_K1159317	.	terminator	1544	1760	.	+	0	ID=BBa_K1159307;Name=BBa_K1159307
BBa_K1159317	.	stop_codon	1544	1760	.	+	0	ID=annotation2341614;Name=CaMV 35S Terminator;Parent=BBa_K1159307
BBa_K1159317	.	engineered_region	1	1535	.	+	0	ID=BBa_K414000;Name=BBa_K414000
BBa_K1159317	.	stop_codon	1530	1535	.	+	0	ID=annotation2087834;Name=Translational Stop 2x;Parent=BBa_K414000
BBa_K1159317	.	start_codon	855	857	.	+	0	ID=annotation2086056;Name=RFP start codon;Parent=BBa_K414000
BBa_K1159317	.	ribosome_entry_site	843	854	.	+	0	ID=annotation2086057;Name=Kozak;Parent=BBa_K414000
BBa_K1159317	.	promoter	1	836	.	+	0	ID=annotation2086059;Name=RD29A;Parent=BBa_K414000
BBa_K1159317	.	CDS	855	1529	.	+	0	ID=annotation2086060;Name=RFP;Parent=BBa_K414000
BBa_K1159317	.	sequence_feature	837	842	.	+	0	ID=annotation2086058;Name=Kpn I;Parent=BBa_K414000
BBa_K1159317	.	sequence_feature	1536	1543	.	+	0	ID=BBa_K1159991;Name=BBa_K1159991
BBa_K1159317	.	sequence_feature	1536	1543	.	+	0	ID=annotation2342822;Name=10/10 NC scar;Parent=BBa_K1159991
BBa_K1159317	.	engineered_region	1769	3285	.	+	0	ID=BBa_K1159308;Name=BBa_K1159308
BBa_K1159317	.	stop_codon	3012	3267	.	+	0	ID=annotation2341662;Name=NOS Terminator;Parent=BBa_K1159308
BBa_K1159317	.	CDS	2142	2936	.	+	0	ID=annotation2341660;Name=Neomycin phosphotransferase II;Parent=BBa_K1159308
BBa_K1159317	.	promoter	1823	2129	.	+	0	ID=annotation2341659;Name=NOS Promoter;Parent=BBa_K1159308
>BBa_K1159317
aggtagaacttatatacattatattgtaattttttgtaacaaaatgtttttattattattatagaattttactggttaa
attaaaaatgaatagaaaaggtgaattaagaggagagaggaggtaaacattttcttctattttttcatattttcaggat
aaattattgtaaaagtttacaagatttccatttgactagggtaaatgaggaatattctctagtaagatcattatttcat
ctacttcttttatcttctaccagtagaggaataaacaatatttagctcctttgtaaatacaaattaattttccttcttg
acatcattcaattttaattttacgtataaaataaaagatcatacctattagaacgattaaggagaaatacaattcgaat
gagaaggatgtgccgtttgttataataaacagccacacgacgtaaacgtaaaatgaccacatgatgggccaatagacat
ggaccgactactaataatagtaagttacattttaggatggaataaatatcataccgacatcagttttgaaagaaaaggg
aaaaaaagaaaaaataaataaaagatatactaccgacatgagttccaaaaagcaaaaaaaaagatcaagccgacacaga
cacgcgtagagagcaaaatgactttgacgtcacaccacgaaaacagacgcttcatacgtgtccctttatctctctcagt
ctctctataaacttagtgagaccctcctctgttttactcacaaatatgcaaactagaaaacaatcatcaggaataaagg
gtttgattacttctattggaaagaaaaaaatctttggaaaatggatggtaccaaaaaaaaaacaatggcctcctccgag
gacgtcatcaaggagttcatgcgcttcaaggtgcgcatggagggctccgtgaacggccacgagttcgagatcgagggcg
agggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggcggccccctgcccttcgcctg
ggacatcctgtcccctcagttccagtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaag
ctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggact
cctccctccaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgca
gaagaagaccatgggctgggaggcctccaccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagatg
aggctgaagctgaaggacggcggccactacgacgccgaggtcaagaccacctacatggccaagaagcccgtgcagctgc
ccggcgcctacaagaccgacatcaagctggacatcacctcccacaacgaggactacaccatcgtggaacagtacgagcg
cgccgagggccgccactccaccggcgcctaataatactagagctagagtccgcaaaaatcaccagtctctctctacaaa
tctatctctctctatttttctccagaataatgtgtgagtagttcccagataagggaattagggttcttatagggtttcg
ctcatgtgttgagcatataagaaacccttagtatgtatttgtatttgtaaaatacttctatcaataaaatttctaattc
ctaaaaccaaaatccagtgacctactagagatcggatcctgtcaaacactgatagtttaaactgaaggcgggaaacgac
aatctgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccgttttacgtttg
gaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggagtttaatgagctaagcacatacgtcag
aaaccattattgcgcgttcaaaagtcgcctaaggtcactatcagctagcaaatatttcttgtcaaaaatgctccactga
cgttccataaattcccctcggtatccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggat
cgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactg
ggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaag
accgacctgtccggtgccctgaatgaattgcaggacgaggcagcgcggctatcgtggctggccacgacgggcgttcctt
gcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcct
gtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggct
acctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcagg
atgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcga
ggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgac
tgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcg
aatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttga
cgagttcttctgagcgggacccaagctttagatcttgctgcgttcggatattttcgtggagttcccgccacagacccgg
atgatccccgatcgttcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatca
tataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttat
gattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgc
gcggtgtcatctatgttactagatcgggcctcctgtcaagcttgat