LOCUS BBa_K1160000 9159 bp DNA linear 22-Dec-2024 DEFINITION coding sequence of Cas9 from CRISPR system type II ACCESSION BBa_K1160000 VERSION BBa_K1160000.1 FEATURES Location/Qualifiers CDS 904..5010 /label=Ca9 coding sequence promoter 615..818 /label=CMV promoter misc_feature 235..614 /label=CMV enhancer misc_feature 5120..5344 /label=bGH poly(A) signal misc_feature 5390..5818 /label=f1 ori misc_feature 6276..6644 /label=BSD misc_feature 7404..7992 /label=ori CDS 8163..9023 /label=AmpR ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 accatggaca agaaatactc tattggactg gatatcggga caaactccgt tggctgggcc 961 gtcataaccg acgagtataa ggtgccaagc aagaaattca aggtgctggg taatactgac 1021 cgccattcaa tcaagaagaa cctgatcgga gcactcctct tcgactccgg tgaaaccgct 1081 gaagctactc ggctgaagcg gaccgcaagg cggagataca cccgccgcaa gaatcggata 1141 tgttatctgc aagagatctt tagcaacgaa atggctaagg tggacgactc cttctttcac 1201 cgcctggaag agagctttct ggtggaggag gataagaaac acgagaggca ccctatattc 1261 ggaaatatcg tggatgaggt ggcttaccat gaaaagtatc ctacaatcta ccatctgagg 1321 aagaagctgg tggacagcac cgataaagca gacctgaggc tcatctatct ggccctggct 1381 catatgataa agtttagagg acactttctg atcgagggcg acctgaatcc cgataattcc 1441 gatgtggata aactcttcat tcaactggtg cagacatata accaactgtt cgaggagaat 1501 cccataaacg cttctggtgt ggatgccaag gctattctgt ccgctcggct gtccaagtca 1561 cgcagactgg agaatctgat tgcccaactg ccaggagaaa agaagaacgg cctgtttggg 1621 aacctcatcg ccctgagcct gggcctgaca cctaacttca agtccaattt tgatctggcc 1681 gaagatgcta aactccagct ctccaaggac acctatgacg atgatctgga caacctgctc 1741 gcacagatag gcgaccagta cgccgatctc tttctggctg ctaagaatct ctccgacgcc 1801 attctgctga gcgacatact ccgggtcaac actgagatca ccaaagcacc tctgagcgcc 1861 tccatgataa aacgctatga tgaacaccat caagacctga ctctgctcaa agccctcgtg 1921 aggcaacagc tgccagagaa gtacaaagag atattcttcg accagagcaa gaatggatat 1981 gccggataca tcgatggcgg agcatcacag gaagaatttt acaagttcat caaaccaatc 2041 ctcgagaaga tggacggtac tgaagagctg ctggtgaagc tgaacaggga ggacctgctg 2101 aggaagcaga ggacctttga taatggctcc attccacatc agatacacct gggagagctg 2161 catgcaatcc tccgcaggca ggaggatttc tatcctttcc tgaaggataa ccgggagaag 2221 atagagaaga tcctgacctt caggatccct tattacgtcg gccctctggc tagaggcaac 2281 tcccgcttcg cttggatgac caggaaatct gaggagacaa ttactccttg gaacttcgaa 2341 gaggtcgtgg ataagggcgc aagcgcccag tcattcatcg aacggatgac caatttcgat 2401 aagaacctgc ccaacgagaa ggtcctgccc aaacattcac tcctgtacga gtatttcacc 2461 gtctataacg agctgactaa agtgaagtac gtgaccgagg gcatgaggaa gcctgccttc 2521 ctgtccggag agcagaagaa ggctatcgtt gatctgctct tcaagactaa tagaaaggtg 2581 acagtgaagc agctcaagga ggattacttt aagaagatcg aatgctttga ctcagtggaa 2641 atctctggcg tggaggaccg ctttaatgcc agcctgggca cttaccatga tctgctgaag 2701 ataatcaaag acaaagattt cctcgataat gaggagaacg aggacatcct ggaagatatc 2761 gtgctgaccc tgactctgtt cgaggataga gagatgatcg aagagcgcct gaagacctat 2821 gcccatctgt ttgacgataa agtcatgaaa cagctcaagc ggcggcgcta cactgggtgg 2881 ggtagactct ccaggaaact cataaacggc atccgcgaca aacagagcgg aaagaccatc 2941 ctggatttcc tgaaatccga cggattcgct aacaggaact tcatgcaact gattcacgat 3001 gactctctga catttaaaga ggacatccag aaggcacagg tgagcggtca aggcgacagc 3061 ctgcacgagc acatcgccaa cctcgctgga tcacccgcca taaagaaggg aatactgcag 3121 acagtcaagg tcgtggacga actcgtcaaa gtgatgggtc ggcacaagcc agagaatatc 3181 gttatcgaaa tggcaaggga gaaccaaacc acccagaagg gccagaagaa ctctcgggaa 3241 cggatgaaaa gaatcgaaga gggaattaag gagctgggat ctcagatact gaaggagcac 3301 cctgtggaga atacacagct ccagaacgag aaactctacc tgtactacct ccagaacggg 3361 cgggacatgt acgttgacca ggaactcgac atcaaccggc tgtccgatta tgacgtggac 3421 catattgttc cacagtcctt cctcaaagat gactccattg acaacaaggt gctgaccaga 3481 tccgataaga atcgcggtaa gtctgacaat gttccatcag aagaggtggt caagaagatg 3541 aagaattact ggcggcagct cctcaacgcc aaactgatca cccagcggaa gtttgacaat 3601 ctgactaagg cagaaagagg aggtctgagc gaactcgaca aggccggctt tattaagagg 3661 caactggtcg aaacacgcca gattaccaaa cacgtggcac aaatcctcga ctctaggatg 3721 aacactaagt acgatgagaa cgataagctg atcagggaag tgaaagtgat aactctgaag 3781 agcaagctgg tgtctgactt ccggaaggac tttcaattct acaaagttcg cgaaataaac 3841 aattaccatc atgctcacga tgcctatctc aatgctgtcg ttggcaccgc cctgatcaag 3901 aaatacccta aactggagtc tgagttcgtg tacggtgact ataaagtcta cgatgtgagg 3961 aagatgatag caaagtctga gcaagagatt ggcaaagcca ccgccaagta cttcttctac 4021 tctaatatca tgaatttctt taagactgag ataaccctgg ctaacggcga aatccggaag 4081 cgcccactga tcgaaacaaa cggagaaaca ggagaaatcg tgtgggataa aggcagggac 4141 ttcgcaactg tgcggaaggt gctgtccatg ccacaagtca atatcgtgaa gaagaccgaa 4201 gtgcagaccg gcggattctc aaaggagagc atcctgccaa agcggaactc tgacaagctg 4261 atcgccagga agaaagattg ggacccaaag aagtatggcg gtttcgattc ccctacagtg 4321 gcttattccg ttctggtcgt ggcaaaagtg gagaaaggca agtccaagaa actcaagtct 4381 gttaaggagc tgctcggaat tactattatg gagagatcca gcttcgagaa gaatccaatc 4441 gatttcctgg aagctaaggg ctataaagaa gtgaagaaag atctcatcat caaactgccc 4501 aagtactctc tctttgagct ggagaatggt aggaagcgga tgctggcctc cgccggagag 4561 ctgcagaaag gaaacgagct ggctctgccc tccaaatacg tgaacttcct gtatctggcc 4621 tcccactacg agaaactcaa aggtagccct gaagacaatg agcagaagca actctttgtt 4681 gagcaacata aacactacct ggacgaaatc attgaacaga ttagcgagtt cagcaagcgg 4741 gttattctgg ccgatgcaaa cctcgataaa gtgctgagcg catataataa gcacagggac 4801 aagccaattc gcgaacaagc agagaatatt atccacctct ttactctgac taatctgggc 4861 gctcctgctg ccttcaagta tttcgataca actattgaca ggaagcggta cacctctacc 4921 aaagaagttc tcgatgccac cctgatacac cagtcaatta ccggactgta cgagactcgc 4981 atcgacctgt ctcagctcgg cggcgactag gggcccttcg aaggtaagcc tatccctaac 5041 cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa 5101 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc 5161 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag 5221 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag 5281 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct 5341 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt 5401 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 5461 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 5521 tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg 5581 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 5641 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 5701 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 5761 gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa 5821 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga 5881 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc 5941 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc 6001 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 6061 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag 6121 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt 6181 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg 6241 gcatagtata atacgacaag gtgaggaact aaaccatggc caagcctttg tctcaagaag 6301 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact 6361 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat 6421 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg 6481 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc 6541 cctgcggacg gtgtcgacag gtgcttctcg atctgcatcc tgggatcaaa gcgatagtga 6601 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg 6661 tgtgggaggg ctaagcactt cgtggccgag gagcaggact gacacgtgct acgagatttc 6721 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 6781 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt 6841 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 6901 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 6961 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg 7021 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 7081 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 7141 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 7201 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 7261 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 7321 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 7381 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 7441 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 7501 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 7561 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc 7621 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 7681 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 7741 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 7801 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 7861 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 7921 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 7981 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 8041 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 8101 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 8161 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 8221 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 8281 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 8341 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 8401 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 8461 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 8521 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 8581 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 8641 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 8701 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 8761 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 8821 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 8881 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 8941 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 9001 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 9061 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 9121 gttccgcgca catttccccg aaaagtgcca cctgacgtc //