LOCUS BBa_K1160001 4900 bp DNA linear 27-Dec-2024 DEFINITION chimeric RNA，combining crRNA and tracrRNA ACCESSION BBa_K1160001 VERSION BBa_K1160001.1 FEATURES Location/Qualifiers misc_feature 4051..4813 /label=Spacers and Repeats promoter 456..966 /label=hPGK promoter CDS 978..1376 /label=BSD misc_feature 1466..1587 /label=SV40 poly(A) misc_feature 2006..2594 /label=ori CDS 2765..3625 /label=AmpR promoter 3626..3730 /label=AmpR promoter ORIGIN 1 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat 61 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt 121 gtggaaagga cgaaacaccg gtgagaccga gagagggtct cagttttaga gctagaaata 181 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt 241 tttttaaaga attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa 301 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca 361 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca 421 gggacagcag agatccactt tggccgcggc tcgagggggt tggggttgcg ccttttccaa 481 ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc 541 ggcgccgacc ctgggactcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc 601 gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt 661 tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc 721 ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc 781 aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag 841 gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc 901 aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct 961 ccccaggggg atccaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa 1021 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag 1081 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac 1141 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt 1201 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgtcgac 1261 aggtgcttct cgatctgcat cctgggatca aagcgatagt gaaggacagt gatggacagc 1321 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaggtac 1381 ctttaagacc aatgacttac aaggcagctg tagatcttag ccactttcta gagtcggggc 1441 ggccggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact 1501 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta 1561 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag 1621 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa 1681 atcgataagg atccgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg 1741 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact 1801 cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 1861 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 1921 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 1981 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 2041 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 2101 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 2161 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc 2221 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 2281 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 2341 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 2401 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 2461 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 2521 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 2581 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 2641 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 2701 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 2761 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 2821 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 2881 gccccagtgc tgcaatgata ccgcgggacc cacgctcacc ggctccagat ttatcagcaa 2941 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 3001 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 3061 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 3121 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 3181 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 3241 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 3301 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 3361 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 3421 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 3481 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 3541 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 3601 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 3661 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 3721 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa 3781 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 3841 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 3901 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 3961 aaaaacttga ttagggtgat ggttcacgag accttctacc gcagaggcgg gggaactcca 4021 agtgatatcc atcatcgcat ccagtgcgcc cggtttatcc ccgctgatgc ggggaacacc 4081 agcgtcaggc gtgaaatctc accgtcgttg ccggtttatc cctgctggcg cggggaactc 4141 tcggttcagg cgttgcaaac ctggctaccg ggcggtttat ccccgctaac gcggggaact 4201 cgtagtccat cattccacct atgtctgaac tcccggttta tccccgctgg cgcggggaac 4261 tcccggggga taatgtttac ggtcatgcgc cccccggttt atccccgctg gcgcggggaa 4321 ctctgggcgg cttgccttgc agccagctcc agcagcggtt tatccccgct ggcgcgggga 4381 actcaagctg gctggcaatc tctttcgggg tgagtccggt ttatccccgc tggcgcgggg 4441 aactctagtt tccgtatctc cggatttata aagctgacgg tttatccccg ctggcgcggg 4501 gaactcgcag gcggcgacgc gcagggtatg cgcgattcgc ggtttatccc cgctggcgcg 4561 gggaactcgc gaccgctcag aaattccaga cccgatccaa acggtttatc cccgctggcg 4621 cggggaactc tcaacattat caattacaac cgacagggag cccggtttat ccccgctggc 4681 gcggggaact cagcgtgttc ggcatcacct ttggcttcgg ctgcggttta tccccgctgg 4741 cgcggggaac tctgcgtgag cgtatcgccg cgcgtctgcg aaagcggttt atccccgctg 4801 gcgcggggaa ctctctaaaa gtatacattt gttcttaaag cattttttcc cataaaaaca 4861 acccaccaac cttaatgtaa catttcctta ttatggtctc //