LOCUS BBa_K1160002 5093 bp DNA linear 29-Dec-2024 DEFINITION cas9 cleavage detector ACCESSION BBa_K1160002 VERSION BBa_K1160002.1 FEATURES Location/Qualifiers CDS 624..1343 /label=eGFP misc_feature 1344..1408 /label=target sequence CDS 2229..3089 /label=AmpR CDS 4382..4756 /label=BleoR misc_feature 3260..3848 /label=ori ORIGIN 1 gcatgcagga atctctggca cggtgctaat ggtagttatc caacggagct gaggtagtcg 61 atatatctgg atatgccgcc tataggataa aaacaggaga gggtgaacct tgcttatggc 121 tactagattg ttcttgtact ctgaattctc attatgggaa actaaactaa tctcatctgt 181 gtgttgcagt actattgaat cgttgtagta tctacctgga gggcattcca tgaattagtg 241 agataacaga gttgggtaac tagagagaat aatagacgta tgcatgatta ctacacaacg 301 gatgtcgcac tctttcctta gttaaaacta tcatccaatc acaagatgcg ggctggaaag 361 acttgctccc gaaggataat cttctgcttc tatctccctt cctcatatgg tttcgcaggg 421 ctcatgcccc ttcttccttc gaactgcccg atgaggaagt ccttagccta tcaaagaatt 481 cgggaccatc atcgattttt agagccttac ctgatcgcaa tcaggatttc actactcata 541 taaatacatc gctcaaagct ccaactttgc ttgttcatac aattcttgat attcacacaa 601 ttgcccttca tatggccaca accatggtga gcaagggcga ggagctgttc accggggtgg 661 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg 721 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca 781 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca 841 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct 901 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg 961 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg 1021 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata 1081 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg 1141 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc 1201 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca 1261 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg 1321 gcatggacga gctgtacaag taatgggcgg cttgccttgc agccagctcc agcagaagct 1381 ggctggcaat ctctttcggg gtgagtcgtc gaccatcatc atcatcatca ttgagtttgt 1441 agccttagac atgactgttc ctcagttcaa gttgggcact tacgagaaga ccggtcttgc 1501 tagattctaa tcaagaggat gtcagaatgc catttgcctg agagatgcag gcttcatttt 1561 tgatactttt ttatttgtaa cctatatagt ataggatttt ttttgtcatt ttgtttcttc 1621 tcgtacgagc ttgctcctga tcagcctatc tcgcagctga tgaatatctt gtggtagggg 1681 tttgggaaaa tcattcgagt ttgatgtttt tcttggtatt tcccactcct cttcagagta 1741 cagaagatta agtgagacct tcgtttgtgc ggatccggcg cgccgatccg cctgatgcgg 1801 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 1861 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg 1921 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg 1981 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc 2041 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag 2101 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 2161 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 2221 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 2281 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 2341 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 2401 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 2461 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 2521 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 2581 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 2641 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 2701 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 2761 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 2821 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 2881 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 2941 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 3001 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 3061 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 3121 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 3181 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 3241 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 3301 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 3361 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 3421 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 3481 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 3541 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 3601 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 3661 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 3721 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 3781 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 3841 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 3901 ctcacacacc atagcttcaa aatgtttcta ctcctttttt actcttccag attttctcgg 3961 actccgcgca tcgccgtacc acttcaaaac acccaagcac agcatactaa atttcccctc 4021 tttcttcctc tagggtgtcg ttaattaccc gtactaaagg tttggaaaag aaaaaagaga 4081 ccgcctcgtt tctttttctt cgtcgaaaaa ggcaataaaa atttttatca cgtttctttt 4141 tcttgaaaat tttttttttt gatttttttc tctttcgatg acctcccatt gatatttaag 4201 ttaataaacg gtcttcaatt tctcaagttt cagtttcatt tttcttgttc tattacaact 4261 ttttttactt cttgctcatt agaaagaaag catagcaatc taatctaagg gcggcgttga 4321 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac 4381 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt 4441 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg 4501 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga 4561 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga 4621 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca 4681 gccgtggggg caggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc 4741 cgaggagcag gactgacacg tccgacgcgg cccgacgggt ccgaggcctc ggagatccgt 4801 cccccttttc ctttgtcgat atcatgtaat tagttatgtc acgcttacat tcacgccctc 4861 cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc taggtcccta 4921 tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa atttttcttt 4981 tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg cttgagaagg 5041 ttttgggacg ctcgaaggct ttaatttgca agctgaattg cggccgcaga tct //