LOCUS       BBa_K116002             1158 bp    DNA     linear       22-Nov-2024
DEFINITION  measurement of NhaA promoter activity
ACCESSION   BBa_K116002
VERSION     BBa_K116002.1  
FEATURES             Location/Qualifiers
     promoter        1..274
                     /label=BBa_K116001
     RBS             283..295
                     /label=RBS-3\Weak
     RBS             289..292
                     /label=RBS
     misc_feature    283..295
                     /label=BBa_B0032
     RBS             283..295
                     /label=BBa_B0032
     CDS             302..1021
                     /label=GFP protein
     CDS             302..1021
                     /label=BBa_E0040
     stem_loop       1041..1084
                     /label=stem_loop
     misc_feature    1030..1109
                     /label=BBa_B0010
     terminator      1030..1109
                     /label=BBa_B0010
     stem_loop       1125..1144
                     /label=T7 TE
     misc_feature    1151..1151
                     /label=stop
     polyA_site      1145..1158
                     /label=polya
     misc_feature    1118..1158
                     /label=BBa_B0012
     terminator      1118..1158
                     /label=BBa_B0012
ORIGIN
        1 attggcctaa caagcatcgc cgactgacaa caaattaatt attacttttc ctaattaatc
       61 cctcaggaat cctcacctta agctatgatt atctaggctt agggtcactc gtgagcgctt
      121 acagccgtca aaaacgcatc tcaccgctga tggcgcaaat tcttcaatag ctcgtaaaaa
      181 acgaattatt cctacactat aatctgattt taacgatgat tcgtgcgggg taaaatagta
      241 aaaacgatct attcacctga aagagaaata aaaatactag agtcacacag gaaagtacta
      301 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      361 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      421 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      481 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
      541 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      601 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      661 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      721 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
      781 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      841 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      901 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
      961 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
     1021 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     1081 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     1141 ggcctttctg cgtttata
//