LOCUS       BBa_K1162012             898 bp    DNA     linear       23-Dec-2024
DEFINITION  GFP with 10x His Tag
ACCESSION   BBa_K1162012
VERSION     BBa_K1162012.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=start
     CDS             1..717
                     /label=BBa_K1162011
     misc_feature    726..755
                     /label=10x-Histidine Tag
     misc_feature    756..758
                     /label=Stop
     misc_feature    759..761
                     /label=Stop
     misc_feature    726..761
                     /label=BBa_K844000
     stem_loop       781..824
                     /label=stem_loop
     misc_feature    770..849
                     /label=BBa_B0010
     terminator      770..849
                     /label=BBa_B0010
     stem_loop       865..884
                     /label=T7 TE
     misc_feature    891..891
                     /label=stop
     polyA_site      885..898
                     /label=polya
     misc_feature    858..898
                     /label=BBa_B0012
     terminator      858..898
                     /label=BBa_B0012
     terminator      770..898
                     /label=BBa_B0015
ORIGIN
        1 atggctagca aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
       61 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgctacatac
      121 ggaaagctta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
      181 cttgtcacta ctttctctta tggtgttcaa tgcttttccc gttatccgga tcatatgaaa
      241 cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaacg cactatatct
      301 ttcaaagatg acgggaacta caagacgcgt gctgaagtca agtttgaagg tgataccctt
      361 gttaatcgta tcgagttaaa aggtattgat tttaaagaag atggaaacat tctcggacac
      421 aaactcgagt acaactataa ctcacacaat gtatacatca cggcagacaa acaaaagaat
      481 ggaatcaaag ctaacttcaa aattcgccac aacattgaag atggatccgt tcaactagca
      541 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
      601 tacctgtcga cacaatctgc cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc
      661 cttcttgagt ttgtaactgc tgctgggatt acacatggca tggatgagct ctacaaatac
      721 tagagcatca tcaccatcac caccatcatc accattaata atactagagc caggcatcaa
      781 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      841 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//