BBa_K844004 1 BBa_K844004 Spider Silk 1x Subunit "B" (Balanced tRNA codon optimized) 2012-10-01T11:00:00Z 2015-05-08T01:13:33Z Artificially designed based on BBa_K844002 and DNA was synthesized Spider silk subunit optimized to use a more balanced, but still greatly reduced, number of tRNA codons; contains two elasticity domains and one strength domain. false false _1104_ 0 13876 9 In stock false none false Andrea Halling annotation2212321 1 Beta-spiral range2212321 1 10 24 annotation2212322 1 Beta-spiral range2212322 1 31 45 annotation2212319 1 Elasticity Domain range2212319 1 1 84 annotation2212324 1 Beta-spiral range2212324 1 69 84 annotation2212330 1 Beta-spiral range2212330 1 153 168 annotation2212323 1 Beta-spiral range2212323 1 46 60 annotation2212332 1 Strength Domain range2212332 1 186 204 annotation2212327 1 Beta-spiral range2212327 1 95 110 annotation2212325 1 Elasticity Domain range2212325 1 85 168 annotation2212326 1 Beta-helix range2212326 1 85 94 annotation2212328 1 Beta-spiral range2212328 1 114 129 annotation2212329 1 Beta-spiral range2212329 1 130 144 annotation2212320 1 Beta-helix range2212320 1 1 9 annotation2212331 1 Linker Domain range2212331 1 169 185 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K1162306 1 BBa_K1162306 Antimicrobial spider silk generator with C-terminus 10x His-Tag 2013-09-16T11:00:00Z 2016-02-10T11:27:24Z Human (Homo sapiens) and Spider (Argiope aurantia) LL-37 + 8x spider silk false false _1474_ 4206 13965 9 It's complicated true RFC 23. In frame fusion protein. false Ryan Putman component2351084 1 BBa_K844004 component2351185 1 BBa_B0015 component2351178 1 BBa_K844000 component2351069 1 BBa_K844004 component2351144 1 BBa_K844004 component2351159 1 BBa_K844004 component2351174 1 BBa_K844004 component2351099 1 BBa_K844004 component2351129 1 BBa_K844004 component2351114 1 BBa_K844004 component2351052 1 BBa_K208010 component2351054 1 BBa_K1162006 annotation2351129 1 BBa_K844004 range2351129 1 1197 1400 annotation2351054 1 BBa_K1162006 range2351054 1 227 340 annotation2351159 1 BBa_K844004 range2351159 1 1621 1824 annotation2351178 1 BBa_K844000 range2351178 1 2045 2080 annotation2351174 1 BBa_K844004 range2351174 1 1833 2036 annotation2351069 1 BBa_K844004 range2351069 1 349 552 annotation2351052 1 BBa_K208010 range2351052 1 1 220 annotation2351114 1 BBa_K844004 range2351114 1 985 1188 annotation2351084 1 BBa_K844004 range2351084 1 561 764 annotation2351185 1 BBa_B0015 range2351185 1 2089 2217 annotation2351144 1 BBa_K844004 range2351144 1 1409 1612 annotation2351099 1 BBa_K844004 range2351099 1 773 976 BBa_K844000 1 BBa_K844000 10x-Histidine (10x-His) Tag with double stop codon (TAATAA) 2012-10-01T11:00:00Z 2015-05-08T01:13:33Z Constructed through oligonucleotide annealing Released HQ 2013 10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part. false false _1104_ 0 9404 9 In stock true none false Kathleen Miller annotation2206607 1 10x-Histidine Tag range2206607 1 1 30 annotation2206608 1 Stop range2206608 1 31 33 annotation2206609 1 Stop range2206609 1 34 36 BBa_K1162006 1 BBa_K1162006 LL-37 antimicrobial peptide from Humans (<i>Homo sapiens</i>) 2013-09-14T11:00:00Z 2016-01-27T02:42:11Z Human (Homo sapiens) This is an antimicrobial peptide (AMP) is commonly found in humans. This part was commercially synthesized and codon optimized for expression in E. coli and is an improvement on LL-37 parts currently in the registry. The prefix and suffix are compatible with RFC 23 (silver fusion) to allow for protein fusions and this part contains an ???atg??? to allow for immediate protein synthesis. Furthermore this LL-37 derivative does not have any stop codons which allows for composite BioBrick Related parts Previously this part has been used by Slovenia iGEM team 2009 (http://parts.igem.org/wiki/index.php?title=Part:BBa_K245114) This part (BBa_K245114) lacks a start codon. Previously this part has been used Trieste iGEM team 2012 http://parts.igem.org/Part:BBa_K875009?title=Part:BBa_K875009 This part, BBa_K875009, has a BioBrick prefix and suffix of RFC 10 so cannot be used for protein fusions, such as in cases of secretion and protein purification. Also has a stop codon which limits what can be added to the C-terminal end (purification tag/secretion tag). false false _1474_ 4206 13965 9 In stock true RFC 23. ATG(Met) added to start of coding region. false Ryan Putman annotation2348651 1 Met range2348651 1 1 3 annotation2365824 1 LL-37 range2365824 1 4 114 BBa_K208010 1 BBa_K208010 Lac Promoter (BBa_R0010) and RBS (B0034) 2009-10-11T11:00:00Z 2015-05-08T01:11:24Z synthetic Released HQ 2013 lac + rbs false false _310_ 0 3473 9 In stock true none false Elisabeth Linton annotation2034625 1 BBa_B0034 range2034625 1 209 220 annotation2034626 1 BBa_R0010 range2034626 1 1 200 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K844004_sequence 1 ggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagca BBa_K1162006_sequence 1 atgctgctgggtgattttttccgcaaaagcaaagaaaaaatcggcaaagagttcaaacgtatcgttcagcgcattaaagattttctgcgtaatctggttccgcgtaccgaaagc BBa_K1162306_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgctgctgggtgattttttccgcaaaagcaaagaaaaaatcggcaaagagttcaaacgtatcgttcagcgcattaaagattttctgcgtaatctggttccgcgtaccgaaagctactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagcatcatcaccatcaccaccatcatcaccattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K844000_sequence 1 catcatcaccatcaccaccatcatcaccattaataa BBa_K208010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z