Antimicrobial spider silk generator with C-terminus 10x His-Tag
Human (Homo sapiens) and Spider (Argiope aurantia)
Double terminator consisting of BBa_B0010 and BBa_B0012
Lac Promoter (BBa_R0010) and RBS (B0034)
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
Constructed through oligonucleotide annealing
10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part.
Spider Silk 1x Subunit "B" (Balanced tRNA codon optimized)
Artificially designed based on BBa_K844002 and DNA was synthesized
Spider silk subunit optimized to use a more balanced, but still greatly reduced, number of tRNA codons; contains two elasticity domains and one strength domain.
LL-37 antimicrobial peptide from Humans (Homo sapiens)
This is an antimicrobial peptide (AMP) is commonly found in humans. This part was commercially synthesized and codon optimized for expression in E. coli and is an improvement on LL-37 parts currently in the registry. The prefix and suffix are compatible with RFC 23 (silver fusion) to allow for protein fusions and this part contains an ???atg??? to allow for immediate protein synthesis. Furthermore this LL-37 derivative does not have any stop codons which allows for composite BioBrick Related parts Previously this part has been used by Slovenia iGEM team 2009 (http://parts.igem.org/wiki/index.php?title=Part:BBa_K245114) This part (BBa_K245114) lacks a start codon. Previously this part has been used Trieste iGEM team 2012 http://parts.igem.org/Part:BBa_K875009?title=Part:BBa_K875009 This part, BBa_K875009, has a BioBrick prefix and suffix of RFC 10 so cannot be used for protein fusions, such as in cases of secretion and protein purification. Also has a stop codon which limits what can be added to the C-terminal end (purification tag/secretion tag).
RFC 23. ATG(Met) added to start of coding region.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1