LOCUS BBa_K1162307 2937 bp DNA linear 26-Nov-2024 DEFINITION WAM-1 antimicrobial peptide fused with 8x spider silk protein, with 10x His-Tag ACCESSION BBa_K1162307 VERSION BBa_K1162307.1 FEATURES Location/Qualifiers RBS 209..220 /label=BBa_B0034 promoter 1..200 /label=BBa_R0010 promoter 1..220 /label=BBa_K208010 misc_feature 227..229 /label=Met CDS 230..337 /label=WAM-1 CDS 227..337 /label=BBa_K1162007 misc_feature 346..429 /label=Elasticity Domain misc_feature 346..354 /label=Beta-helix misc_feature 355..369 /label=Beta-spiral misc_feature 376..390 /label=Beta-spiral misc_feature 391..405 /label=Beta-spiral misc_feature 414..429 /label=Beta-spiral misc_feature 430..513 /label=Elasticity Domain misc_feature 430..439 /label=Beta-helix misc_feature 440..455 /label=Beta-spiral misc_feature 459..474 /label=Beta-spiral misc_feature 475..489 /label=Beta-spiral misc_feature 498..513 /label=Beta-spiral misc_feature 514..530 /label=Linker Domain misc_feature 531..549 /label=Strength Domain CDS 346..549 /label=BBa_K844004 misc_feature 558..641 /label=Elasticity Domain misc_feature 558..566 /label=Beta-helix misc_feature 567..581 /label=Beta-spiral misc_feature 588..602 /label=Beta-spiral misc_feature 603..617 /label=Beta-spiral misc_feature 626..641 /label=Beta-spiral misc_feature 642..725 /label=Elasticity Domain misc_feature 642..651 /label=Beta-helix misc_feature 652..667 /label=Beta-spiral misc_feature 671..686 /label=Beta-spiral misc_feature 687..701 /label=Beta-spiral misc_feature 710..725 /label=Beta-spiral misc_feature 726..742 /label=Linker Domain misc_feature 743..761 /label=Strength Domain CDS 558..761 /label=BBa_K844004 misc_feature 770..853 /label=Elasticity Domain misc_feature 770..778 /label=Beta-helix misc_feature 779..793 /label=Beta-spiral misc_feature 800..814 /label=Beta-spiral misc_feature 815..829 /label=Beta-spiral misc_feature 838..853 /label=Beta-spiral misc_feature 854..937 /label=Elasticity Domain misc_feature 854..863 /label=Beta-helix misc_feature 864..879 /label=Beta-spiral misc_feature 883..898 /label=Beta-spiral misc_feature 899..913 /label=Beta-spiral misc_feature 922..937 /label=Beta-spiral misc_feature 938..954 /label=Linker Domain misc_feature 955..973 /label=Strength Domain CDS 770..973 /label=BBa_K844004 misc_feature 982..1065 /label=Elasticity Domain misc_feature 982..990 /label=Beta-helix misc_feature 991..1005 /label=Beta-spiral misc_feature 1012..1026 /label=Beta-spiral misc_feature 1027..1041 /label=Beta-spiral misc_feature 1050..1065 /label=Beta-spiral misc_feature 1066..1149 /label=Elasticity Domain misc_feature 1066..1075 /label=Beta-helix misc_feature 1076..1091 /label=Beta-spiral misc_feature 1095..1110 /label=Beta-spiral misc_feature 1111..1125 /label=Beta-spiral misc_feature 1134..1149 /label=Beta-spiral misc_feature 1150..1166 /label=Linker Domain misc_feature 1167..1185 /label=Strength Domain CDS 982..1185 /label=BBa_K844004 misc_feature 1194..1277 /label=Elasticity Domain misc_feature 1194..1202 /label=Beta-helix misc_feature 1203..1217 /label=Beta-spiral misc_feature 1224..1238 /label=Beta-spiral misc_feature 1239..1253 /label=Beta-spiral misc_feature 1262..1277 /label=Beta-spiral misc_feature 1278..1361 /label=Elasticity Domain misc_feature 1278..1287 /label=Beta-helix misc_feature 1288..1303 /label=Beta-spiral misc_feature 1307..1322 /label=Beta-spiral misc_feature 1323..1337 /label=Beta-spiral misc_feature 1346..1361 /label=Beta-spiral misc_feature 1362..1378 /label=Linker Domain misc_feature 1379..1397 /label=Strength Domain CDS 1194..1397 /label=BBa_K844004 misc_feature 1406..1489 /label=Elasticity Domain misc_feature 1406..1414 /label=Beta-helix misc_feature 1415..1429 /label=Beta-spiral misc_feature 1436..1450 /label=Beta-spiral misc_feature 1451..1465 /label=Beta-spiral misc_feature 1474..1489 /label=Beta-spiral misc_feature 1490..1573 /label=Elasticity Domain misc_feature 1490..1499 /label=Beta-helix misc_feature 1500..1515 /label=Beta-spiral misc_feature 1519..1534 /label=Beta-spiral misc_feature 1535..1549 /label=Beta-spiral misc_feature 1558..1573 /label=Beta-spiral misc_feature 1574..1590 /label=Linker Domain misc_feature 1591..1609 /label=Strength Domain CDS 1406..1609 /label=BBa_K844004 misc_feature 1618..1701 /label=Elasticity Domain misc_feature 1618..1626 /label=Beta-helix misc_feature 1627..1641 /label=Beta-spiral misc_feature 1648..1662 /label=Beta-spiral misc_feature 1663..1677 /label=Beta-spiral misc_feature 1686..1701 /label=Beta-spiral misc_feature 1702..1785 /label=Elasticity Domain misc_feature 1702..1711 /label=Beta-helix misc_feature 1712..1727 /label=Beta-spiral misc_feature 1731..1746 /label=Beta-spiral misc_feature 1747..1761 /label=Beta-spiral misc_feature 1770..1785 /label=Beta-spiral misc_feature 1786..1802 /label=Linker Domain misc_feature 1803..1821 /label=Strength Domain CDS 1618..1821 /label=BBa_K844004 misc_feature 1830..1913 /label=Elasticity Domain misc_feature 1830..1838 /label=Beta-helix misc_feature 1839..1853 /label=Beta-spiral misc_feature 1860..1874 /label=Beta-spiral misc_feature 1875..1889 /label=Beta-spiral misc_feature 1898..1913 /label=Beta-spiral misc_feature 1914..1997 /label=Elasticity Domain misc_feature 1914..1923 /label=Beta-helix misc_feature 1924..1939 /label=Beta-spiral misc_feature 1943..1958 /label=Beta-spiral misc_feature 1959..1973 /label=Beta-spiral misc_feature 1982..1997 /label=Beta-spiral misc_feature 1998..2014 /label=Linker Domain misc_feature 2015..2033 /label=Strength Domain CDS 1830..2033 /label=BBa_K844004 misc_feature 2040..2042 /label=start CDS 2040..2756 /label=BBa_K1162011 misc_feature 2765..2794 /label=10x-Histidine Tag misc_feature 2795..2797 /label=Stop misc_feature 2798..2800 /label=Stop misc_feature 2765..2800 /label=BBa_K844000 stem_loop 2820..2863 /label=stem_loop misc_feature 2809..2888 /label=BBa_B0010 terminator 2809..2888 /label=BBa_B0010 stem_loop 2904..2923 /label=T7 TE misc_feature 2930..2930 /label=stop polyA_site 2924..2937 /label=polya misc_feature 2897..2937 /label=BBa_B0012 terminator 2897..2937 /label=BBa_B0012 terminator 2809..2937 /label=BBa_B0015 misc_feature 2040..2937 /label=BBa_K1162012 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aacgcggttt 241 tggtaaaaaa ctgcgtaaac gcctgaaaaa attccgcaac agcatcaaaa aacgtctgaa 301 aaactttaac gtggtgattc cgattccgct gcctggttac tagagggtgg atatggtcct 361 ggagcaggtc aacaaggacc aggtagtcaa ggacctggtt ctggaggtca acaaggacca 421 ggtggacaag gtggatatgg tcctggagca ggtcaacaag gaccaggtag tcaaggacct 481 ggttctggag gtcaacaagg accaggtgga caaggtcctt atggaccaag tgcagcagca 541 gcagcagcat actagagggt ggatatggtc ctggagcagg tcaacaagga ccaggtagtc 601 aaggacctgg ttctggaggt caacaaggac caggtggaca aggtggatat ggtcctggag 661 caggtcaaca aggaccaggt agtcaaggac ctggttctgg aggtcaacaa ggaccaggtg 721 gacaaggtcc ttatggacca agtgcagcag cagcagcagc atactagagg gtggatatgg 781 tcctggagca ggtcaacaag gaccaggtag tcaaggacct ggttctggag gtcaacaagg 841 accaggtgga caaggtggat atggtcctgg agcaggtcaa caaggaccag gtagtcaagg 901 acctggttct ggaggtcaac aaggaccagg tggacaaggt ccttatggac caagtgcagc 961 agcagcagca gcatactaga gggtggatat ggtcctggag caggtcaaca aggaccaggt 1021 agtcaaggac ctggttctgg aggtcaacaa ggaccaggtg gacaaggtgg atatggtcct 1081 ggagcaggtc aacaaggacc aggtagtcaa ggacctggtt ctggaggtca acaaggacca 1141 ggtggacaag gtccttatgg accaagtgca gcagcagcag cagcatacta gagggtggat 1201 atggtcctgg agcaggtcaa caaggaccag gtagtcaagg acctggttct ggaggtcaac 1261 aaggaccagg tggacaaggt ggatatggtc ctggagcagg tcaacaagga ccaggtagtc 1321 aaggacctgg ttctggaggt caacaaggac caggtggaca aggtccttat ggaccaagtg 1381 cagcagcagc agcagcatac tagagggtgg atatggtcct ggagcaggtc aacaaggacc 1441 aggtagtcaa ggacctggtt ctggaggtca acaaggacca ggtggacaag gtggatatgg 1501 tcctggagca ggtcaacaag gaccaggtag tcaaggacct ggttctggag gtcaacaagg 1561 accaggtgga caaggtcctt atggaccaag tgcagcagca gcagcagcat actagagggt 1621 ggatatggtc ctggagcagg tcaacaagga ccaggtagtc aaggacctgg ttctggaggt 1681 caacaaggac caggtggaca aggtggatat ggtcctggag caggtcaaca aggaccaggt 1741 agtcaaggac ctggttctgg aggtcaacaa ggaccaggtg gacaaggtcc ttatggacca 1801 agtgcagcag cagcagcagc atactagagg gtggatatgg tcctggagca ggtcaacaag 1861 gaccaggtag tcaaggacct ggttctggag gtcaacaagg accaggtgga caaggtggat 1921 atggtcctgg agcaggtcaa caaggaccag gtagtcaagg acctggttct ggaggtcaac 1981 aaggaccagg tggacaaggt ccttatggac caagtgcagc agcagcagca gcatactaga 2041 tggctagcaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg 2101 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gctacatacg 2161 gaaagcttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac 2221 ttgtcactac tttctcttat ggtgttcaat gcttttcccg ttatccggat catatgaaac 2281 ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaacgc actatatctt 2341 tcaaagatga cgggaactac aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg 2401 ttaatcgtat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt ctcggacaca 2461 aactcgagta caactataac tcacacaatg tatacatcac ggcagacaaa caaaagaatg 2521 gaatcaaagc taacttcaaa attcgccaca acattgaaga tggatccgtt caactagcag 2581 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt 2641 acctgtcgac acaatctgcc ctttcgaaag atcccaacga aaagcgtgac cacatggtcc 2701 ttcttgagtt tgtaactgct gctgggatta cacatggcat ggatgagctc tacaaatact 2761 agagcatcat caccatcacc accatcatca ccattaataa tactagagcc aggcatcaaa 2821 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 2881 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //