##gff-version 3 ##sequence-region BBa_K1162307 1 2937 BBa_K1162307 . CDS 346 549 . + 0 ID=BBa_K844004;Name=BBa_K844004 BBa_K1162307 . sequence_feature 376 390 . + 0 ID=annotation2212322;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 430 513 . + 0 ID=annotation2212325;Name=Elasticity Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 346 429 . + 0 ID=annotation2212319;Name=Elasticity Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 414 429 . + 0 ID=annotation2212324;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 355 369 . + 0 ID=annotation2212321;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 498 513 . + 0 ID=annotation2212330;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 531 549 . + 0 ID=annotation2212332;Name=Strength Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 440 455 . + 0 ID=annotation2212327;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 430 439 . + 0 ID=annotation2212326;Name=Beta-helix;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 459 474 . + 0 ID=annotation2212328;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 346 354 . + 0 ID=annotation2212320;Name=Beta-helix;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 514 530 . + 0 ID=annotation2212331;Name=Linker Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 475 489 . + 0 ID=annotation2212329;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 391 405 . + 0 ID=annotation2212323;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . CDS 1618 1821 . + 0 ID=BBa_K844004;Name=BBa_K844004 BBa_K1162307 . sequence_feature 1648 1662 . + 0 ID=annotation2212322;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1702 1785 . + 0 ID=annotation2212325;Name=Elasticity Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1618 1701 . + 0 ID=annotation2212319;Name=Elasticity Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1686 1701 . + 0 ID=annotation2212324;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1627 1641 . + 0 ID=annotation2212321;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1770 1785 . + 0 ID=annotation2212330;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1803 1821 . + 0 ID=annotation2212332;Name=Strength Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1712 1727 . + 0 ID=annotation2212327;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1702 1711 . + 0 ID=annotation2212326;Name=Beta-helix;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1731 1746 . + 0 ID=annotation2212328;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1618 1626 . + 0 ID=annotation2212320;Name=Beta-helix;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1786 1802 . + 0 ID=annotation2212331;Name=Linker Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1747 1761 . + 0 ID=annotation2212329;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1663 1677 . + 0 ID=annotation2212323;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . CDS 227 337 . + 0 ID=BBa_K1162007;Name=BBa_K1162007 BBa_K1162307 . start_codon 227 229 . + 0 ID=annotation2362765;Name=Met;Parent=BBa_K1162007 BBa_K1162307 . CDS 230 337 . + 0 ID=annotation2362766;Name=WAM-1;Parent=BBa_K1162007 BBa_K1162307 . CDS 558 761 . + 0 ID=BBa_K844004;Name=BBa_K844004 BBa_K1162307 . sequence_feature 588 602 . + 0 ID=annotation2212322;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 642 725 . + 0 ID=annotation2212325;Name=Elasticity Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 558 641 . + 0 ID=annotation2212319;Name=Elasticity Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 626 641 . + 0 ID=annotation2212324;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 567 581 . + 0 ID=annotation2212321;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 710 725 . + 0 ID=annotation2212330;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 743 761 . + 0 ID=annotation2212332;Name=Strength Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 652 667 . + 0 ID=annotation2212327;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 642 651 . + 0 ID=annotation2212326;Name=Beta-helix;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 671 686 . + 0 ID=annotation2212328;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 558 566 . + 0 ID=annotation2212320;Name=Beta-helix;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 726 742 . + 0 ID=annotation2212331;Name=Linker Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 687 701 . + 0 ID=annotation2212329;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 603 617 . + 0 ID=annotation2212323;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . CDS 1830 2033 . + 0 ID=BBa_K844004;Name=BBa_K844004 BBa_K1162307 . sequence_feature 1860 1874 . + 0 ID=annotation2212322;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1914 1997 . + 0 ID=annotation2212325;Name=Elasticity Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1830 1913 . + 0 ID=annotation2212319;Name=Elasticity Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1898 1913 . + 0 ID=annotation2212324;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1839 1853 . + 0 ID=annotation2212321;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1982 1997 . + 0 ID=annotation2212330;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 2015 2033 . + 0 ID=annotation2212332;Name=Strength Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1924 1939 . + 0 ID=annotation2212327;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1914 1923 . + 0 ID=annotation2212326;Name=Beta-helix;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1943 1958 . + 0 ID=annotation2212328;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1830 1838 . + 0 ID=annotation2212320;Name=Beta-helix;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1998 2014 . + 0 ID=annotation2212331;Name=Linker Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1959 1973 . + 0 ID=annotation2212329;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1875 1889 . + 0 ID=annotation2212323;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . promoter 1 220 . + 0 ID=BBa_K208010;Name=BBa_K208010 BBa_K1162307 . ribosome_entry_site 209 220 . + 0 ID=annotation2034625;Name=BBa_B0034;Parent=BBa_K208010 BBa_K1162307 . promoter 1 200 . + 0 ID=annotation2034626;Name=BBa_R0010;Parent=BBa_K208010 BBa_K1162307 . engineered_region 2040 2937 . + 0 ID=BBa_K1162012;Name=BBa_K1162012 BBa_K1162307 . tag 2765 2800 . + 0 ID=BBa_K844000;Name=BBa_K844000;Parent=BBa_K1162012 BBa_K1162307 . sequence_feature 2765 2794 . + 0 ID=annotation2206607;Name=10x-Histidine Tag;Parent=BBa_K844000 BBa_K1162307 . stop_codon 2798 2800 . + 0 ID=annotation2206609;Name=Stop;Parent=BBa_K844000 BBa_K1162307 . stop_codon 2795 2797 . + 0 ID=annotation2206608;Name=Stop;Parent=BBa_K844000 BBa_K1162307 . terminator 2809 2937 . + 0 ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1162012 BBa_K1162307 . terminator 2809 2888 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K1162307 . engineered_region 2809 2888 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K1162307 . stem_loop 2820 2863 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K1162307 . terminator 2897 2937 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K1162307 . stop_codon 2930 2930 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1162307 . polyA_site 2924 2937 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1162307 . stem_loop 2904 2923 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1162307 . engineered_region 2897 2937 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1162307 . CDS 2040 2756 . + 0 ID=BBa_K1162011;Name=BBa_K1162011;Parent=BBa_K1162012 BBa_K1162307 . start_codon 2040 2042 . + 0 ID=annotation2343983;Name=start;Parent=BBa_K1162011 BBa_K1162307 . CDS 982 1185 . + 0 ID=BBa_K844004;Name=BBa_K844004 BBa_K1162307 . sequence_feature 1012 1026 . + 0 ID=annotation2212322;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1066 1149 . + 0 ID=annotation2212325;Name=Elasticity Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 982 1065 . + 0 ID=annotation2212319;Name=Elasticity Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1050 1065 . + 0 ID=annotation2212324;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 991 1005 . + 0 ID=annotation2212321;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1134 1149 . + 0 ID=annotation2212330;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1167 1185 . + 0 ID=annotation2212332;Name=Strength Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1076 1091 . + 0 ID=annotation2212327;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1066 1075 . + 0 ID=annotation2212326;Name=Beta-helix;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1095 1110 . + 0 ID=annotation2212328;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 982 990 . + 0 ID=annotation2212320;Name=Beta-helix;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1150 1166 . + 0 ID=annotation2212331;Name=Linker Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1111 1125 . + 0 ID=annotation2212329;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1027 1041 . + 0 ID=annotation2212323;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . CDS 770 973 . + 0 ID=BBa_K844004;Name=BBa_K844004 BBa_K1162307 . sequence_feature 800 814 . + 0 ID=annotation2212322;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 854 937 . + 0 ID=annotation2212325;Name=Elasticity Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 770 853 . + 0 ID=annotation2212319;Name=Elasticity Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 838 853 . + 0 ID=annotation2212324;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 779 793 . + 0 ID=annotation2212321;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 922 937 . + 0 ID=annotation2212330;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 955 973 . + 0 ID=annotation2212332;Name=Strength Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 864 879 . + 0 ID=annotation2212327;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 854 863 . + 0 ID=annotation2212326;Name=Beta-helix;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 883 898 . + 0 ID=annotation2212328;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 770 778 . + 0 ID=annotation2212320;Name=Beta-helix;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 938 954 . + 0 ID=annotation2212331;Name=Linker Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 899 913 . + 0 ID=annotation2212329;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 815 829 . + 0 ID=annotation2212323;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . CDS 1194 1397 . + 0 ID=BBa_K844004;Name=BBa_K844004 BBa_K1162307 . sequence_feature 1224 1238 . + 0 ID=annotation2212322;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1278 1361 . + 0 ID=annotation2212325;Name=Elasticity Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1194 1277 . + 0 ID=annotation2212319;Name=Elasticity Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1262 1277 . + 0 ID=annotation2212324;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1203 1217 . + 0 ID=annotation2212321;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1346 1361 . + 0 ID=annotation2212330;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1379 1397 . + 0 ID=annotation2212332;Name=Strength Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1288 1303 . + 0 ID=annotation2212327;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1278 1287 . + 0 ID=annotation2212326;Name=Beta-helix;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1307 1322 . + 0 ID=annotation2212328;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1194 1202 . + 0 ID=annotation2212320;Name=Beta-helix;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1362 1378 . + 0 ID=annotation2212331;Name=Linker Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1323 1337 . + 0 ID=annotation2212329;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1239 1253 . + 0 ID=annotation2212323;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . CDS 1406 1609 . + 0 ID=BBa_K844004;Name=BBa_K844004 BBa_K1162307 . sequence_feature 1436 1450 . + 0 ID=annotation2212322;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1490 1573 . + 0 ID=annotation2212325;Name=Elasticity Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1406 1489 . + 0 ID=annotation2212319;Name=Elasticity Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1474 1489 . + 0 ID=annotation2212324;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1415 1429 . + 0 ID=annotation2212321;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1558 1573 . + 0 ID=annotation2212330;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1591 1609 . + 0 ID=annotation2212332;Name=Strength Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1500 1515 . + 0 ID=annotation2212327;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1490 1499 . + 0 ID=annotation2212326;Name=Beta-helix;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1519 1534 . + 0 ID=annotation2212328;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1406 1414 . + 0 ID=annotation2212320;Name=Beta-helix;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1574 1590 . + 0 ID=annotation2212331;Name=Linker Domain;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1535 1549 . + 0 ID=annotation2212329;Name=Beta-spiral;Parent=BBa_K844004 BBa_K1162307 . sequence_feature 1451 1465 . + 0 ID=annotation2212323;Name=Beta-spiral;Parent=BBa_K844004 >BBa_K1162307 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaacgcgg ttttggtaaaaaactgcgtaaacgcctgaaaaaattccgcaacagcatcaaaaaacgtctgaaaaactttaacgtggtg attccgattccgctgcctggttactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacc tggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaa ggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatact agagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggacca ggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaag gaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagca ggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctg gagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatgg accaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaag gacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtag tcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagca tactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaagg accaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaa caaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctgg agcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggt cctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtcctt atggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagt caaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccag gtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagc agcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaac aaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggagg tcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagatggctagcaaagga gaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtg gagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatg gccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgacttt ttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtg ctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacat tctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaa gctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattg gcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcg tgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaatactagagc atcatcaccatcaccaccatcatcaccattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaag actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc tttctgcgtttata