BBa_K844004 1 BBa_K844004 Spider Silk 1x Subunit "B" (Balanced tRNA codon optimized) 2012-10-01T11:00:00Z 2015-05-08T01:13:33Z Artificially designed based on BBa_K844002 and DNA was synthesized Spider silk subunit optimized to use a more balanced, but still greatly reduced, number of tRNA codons; contains two elasticity domains and one strength domain. false false _1104_ 0 13876 9 In stock false none false Andrea Halling annotation2212321 1 Beta-spiral range2212321 1 10 24 annotation2212322 1 Beta-spiral range2212322 1 31 45 annotation2212319 1 Elasticity Domain range2212319 1 1 84 annotation2212324 1 Beta-spiral range2212324 1 69 84 annotation2212330 1 Beta-spiral range2212330 1 153 168 annotation2212323 1 Beta-spiral range2212323 1 46 60 annotation2212332 1 Strength Domain range2212332 1 186 204 annotation2212327 1 Beta-spiral range2212327 1 95 110 annotation2212325 1 Elasticity Domain range2212325 1 85 168 annotation2212326 1 Beta-helix range2212326 1 85 94 annotation2212328 1 Beta-spiral range2212328 1 114 129 annotation2212329 1 Beta-spiral range2212329 1 130 144 annotation2212320 1 Beta-helix range2212320 1 1 9 annotation2212331 1 Linker Domain range2212331 1 169 185 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K844000 1 BBa_K844000 10x-Histidine (10x-His) Tag with double stop codon (TAATAA) 2012-10-01T11:00:00Z 2015-05-08T01:13:33Z Constructed through oligonucleotide annealing Released HQ 2013 10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part. false false _1104_ 0 9404 9 In stock true none false Kathleen Miller annotation2206607 1 10x-Histidine Tag range2206607 1 1 30 annotation2206608 1 Stop range2206608 1 31 33 annotation2206609 1 Stop range2206609 1 34 36 BBa_K1162011 1 BBa_K1162011 GFP with stop codon removed 2013-09-14T11:00:00Z 2015-05-08T01:09:32Z E. coli Improves upon the original GFP (BBa_K208000) by removing the stop codon. This allows for C terminal addition to this construct. For example purification/secretion tag or a protein fusion with GFP. This GFP confers to RFC 23 silver fusion standard. false false _1474_ 0 9404 9 It's complicated false RFC 23 standards used. Stop codon from BBa_K208000 removed. false Kathleen Miller annotation2343983 1 start range2343983 1 1 3 BBa_K208010 1 BBa_K208010 Lac Promoter (BBa_R0010) and RBS (B0034) 2009-10-11T11:00:00Z 2015-05-08T01:11:24Z synthetic Released HQ 2013 lac + rbs false false _310_ 0 3473 9 In stock true none false Elisabeth Linton annotation2034625 1 BBa_B0034 range2034625 1 209 220 annotation2034626 1 BBa_R0010 range2034626 1 1 200 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_K1162012 1 BBa_K1162012 GFP with 10x His Tag 2013-09-14T11:00:00Z 2015-05-08T01:09:32Z E. coli. The GFP present in this part is modified from (BBa_K208000 http://parts.igem.org/wiki/index.php?title=Part:BBa_K208000), with stop codon removed to allow for full translation to 10x His Tag, BBa_K844000 (http://parts.igem.org/wiki/index.php?title=Part:BBa_K844000). This part is useful as it allows the user to carry out gene expression studies and purify the final protein-GFP-His Tag fusion if desired. The prefix in front of the GFP is RFC 23 compatible to allow for in-frame expression of genes. Once protein expression has been demonstrated the gene can be put in front of BBa_K844000 for protein purification without the GFP. false false _1474_ 0 9404 9 It's complicated false RFC 23 with GFP and 10x His Tag fusion. false Kathleen Miller component2344030 1 BBa_K844000 component2344026 1 BBa_K1162011 component2344037 1 BBa_B0015 annotation2344037 1 BBa_B0015 range2344037 1 770 898 annotation2344030 1 BBa_K844000 range2344030 1 726 761 annotation2344026 1 BBa_K1162011 range2344026 1 1 717 BBa_K1162307 1 BBa_K1162307 WAM-1 antimicrobial peptide fused with 8x spider silk protein, with 10x His-Tag 2013-09-24T11:00:00Z 2015-05-08T01:09:32Z Composite of genes from tammar wallaby and Argiope aurantia spider WAM-1 with 8x spider silk, 10x His-Tag false false _1474_ 0 13965 9 Not in stock false RFC 23. ATG (Met) at start of coding region. false Ryan Putman component2363142 1 BBa_K844004 component2363127 1 BBa_K844004 component2363052 1 BBa_K1162007 component2363112 1 BBa_K844004 component2363157 1 BBa_K844004 component2363186 1 BBa_K1162012 component2363067 1 BBa_K844004 component2363082 1 BBa_K844004 component2363097 1 BBa_K844004 component2363049 1 BBa_K208010 component2363172 1 BBa_K844004 annotation2363112 1 BBa_K844004 range2363112 1 982 1185 annotation2363082 1 BBa_K844004 range2363082 1 558 761 annotation2363052 1 BBa_K1162007 range2363052 1 227 337 annotation2363097 1 BBa_K844004 range2363097 1 770 973 annotation2363067 1 BBa_K844004 range2363067 1 346 549 annotation2363049 1 BBa_K208010 range2363049 1 1 220 annotation2363127 1 BBa_K844004 range2363127 1 1194 1397 annotation2363157 1 BBa_K844004 range2363157 1 1618 1821 annotation2363186 1 BBa_K1162012 range2363186 1 2040 2937 annotation2363172 1 BBa_K844004 range2363172 1 1830 2033 annotation2363142 1 BBa_K844004 range2363142 1 1406 1609 BBa_K1162007 1 BBa_K1162007 WAM-1 antimicrobial peptide from the tammar wallaby (<i>Macropus eugenii</i>) 2013-09-14T11:00:00Z 2016-01-28T12:14:14Z Tammar Wallaby (Macropus eugenii) An antimicrobial peptide from the Cathelicidin family of peptides, isolated from the Tammar Wallaby (Macropus eugenii). ATG(Met) added at start of coding region. false false _1474_ 4206 13965 9 In stock false RFC 23. false Ryan Putman annotation2362765 1 Met range2362765 1 1 3 annotation2362766 1 WAM-1 range2362766 1 4 111 BBa_K1162012_sequence 1 atggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaatactagagcatcatcaccatcaccaccatcatcaccattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K844004_sequence 1 ggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagca BBa_K1162011_sequence 1 atggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaa BBa_K844000_sequence 1 catcatcaccatcaccaccatcatcaccattaataa BBa_K208010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1162007_sequence 1 atgaaacgcggttttggtaaaaaactgcgtaaacgcctgaaaaaattccgcaacagcatcaaaaaacgtctgaaaaactttaacgtggtgattccgattccgctgcctggt BBa_K1162307_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaacgcggttttggtaaaaaactgcgtaaacgcctgaaaaaattccgcaacagcatcaaaaaacgtctgaaaaactttaacgtggtgattccgattccgctgcctggttactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagatggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaatactagagcatcatcaccatcaccaccatcatcaccattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z