WAM-1 antimicrobial peptide fused with 8x spider silk protein, with 10x His-Tag
Composite of genes from tammar wallaby and Argiope aurantia spider
RFC 23. ATG (Met) at start of coding region.
Lac Promoter (BBa_R0010) and RBS (B0034)
Spider Silk 1x Subunit "B" (Balanced tRNA codon optimized)
Artificially designed based on BBa_K844002 and DNA was synthesized
Spider silk subunit optimized to use a more balanced, but still greatly reduced, number of tRNA codons; contains two elasticity domains and one strength domain.
WAM-1 antimicrobial peptide from the tammar wallaby (Macropus eugenii)
An antimicrobial peptide from the Cathelicidin family of peptides, isolated from the Tammar Wallaby (Macropus eugenii). ATG(Met) added at start of coding region.
The GFP present in this part is modified from (BBa_K208000 http://parts.igem.org/wiki/index.php?title=Part:BBa_K208000), with stop codon removed to allow for full translation to 10x His Tag, BBa_K844000 (http://parts.igem.org/wiki/index.php?title=Part:BBa_K844000). This part is useful as it allows the user to carry out gene expression studies and purify the final protein-GFP-His Tag fusion if desired. The prefix in front of the GFP is RFC 23 compatible to allow for in-frame expression of genes. Once protein expression has been demonstrated the gene can be put in front of BBa_K844000 for protein purification without the GFP.
Double terminator consisting of BBa_B0010 and BBa_B0012
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
Constructed through oligonucleotide annealing
10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part.
Improves upon the original GFP (BBa_K208000) by removing the stop codon. This allows for C terminal addition to this construct. For example purification/secretion tag or a protein fusion with GFP. This GFP confers to RFC 23 silver fusion standard.
RFC 23 standards used. Stop codon from BBa_K208000 removed.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1