BBa_K1162307
type: ComponentDefinition
persistent identity: BBa K1162307
display id: BBa_K1162307
version: 1
was generated by: iGEM to SBOL conversion
description: WAM-1 antimicrobial peptide fused with 8x spider silk protein, with 10x His-Tag
created: 2013-09-24T11:00:00Z
modified: 2015-05-08T01:09:32Z
mutable provenance: Composite of genes from tammar wallaby and Argiope aurantia spider
owned by: james
owned by: myers
mutable description: WAM-1 with 8x spider silk, 10x His-Tag
discontinued: false
dominant: false
group u list: _1474_
m user id: 0
owner id: 13965
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: RFC 23. ATG (Met) at start of coding region.
star: false
creator: Ryan Putman
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1162012
component: BBa_K844004
component: BBa_K208010
component: BBa_K1162007
sequence annotation: BBa_K208010
sequence annotation: BBa_K1162007
sequence annotation: BBa_K844004
sequence annotation: BBa_K1162012
sequence: https://synbiohub.org/public/igem/BBa_K1162307_sequence/1

BBa_K1162012
type: Component
persistent identity: component2363186
display id: component2363186
version: 1
top level: BBa_K1162307
definition: BBa_K1162012
access: public

BBa_K844004
type: Component
persistent identity: component2363172
display id: component2363172
version: 1
top level: BBa_K1162307
definition: BBa_K844004
access: public

BBa_K208010
type: Component
persistent identity: component2363049
display id: component2363049
version: 1
top level: BBa_K1162307
definition: BBa_K208010
access: public

BBa_K844004
type: Component
persistent identity: component2363067
display id: component2363067
version: 1
top level: BBa_K1162307
definition: BBa_K844004
access: public

BBa_K1162007
type: Component
persistent identity: component2363052
display id: component2363052
version: 1
top level: BBa_K1162307
definition: BBa_K1162007
access: public

BBa_K844004
type: Component
persistent identity: component2363097
display id: component2363097
version: 1
top level: BBa_K1162307
definition: BBa_K844004
access: public

BBa_K844004
type: Component
persistent identity: component2363082
display id: component2363082
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definition: BBa_K844004
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BBa_K844004
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persistent identity: component2363127
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definition: BBa_K844004
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BBa_K844004
type: Component
persistent identity: component2363112
display id: component2363112
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definition: BBa_K844004
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BBa_K844004
type: Component
persistent identity: component2363157
display id: component2363157
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definition: BBa_K844004
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BBa_K844004
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persistent identity: component2363142
display id: component2363142
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definition: BBa_K844004
access: public

BBa_K208010
type: SequenceAnnotation
persistent identity: annotation2363049
display id: annotation2363049
version: 1
top level: BBa_K1162307
location: https://synbiohub.org/public/igem/BBa_K1162307/annotation2363049/range2363049/1
component: BBa_K208010

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BBa_K1162007
type: SequenceAnnotation
persistent identity: annotation2363052
display id: annotation2363052
version: 1
top level: BBa_K1162307
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BBa_K844004
type: SequenceAnnotation
persistent identity: annotation2363067
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version: 1
top level: BBa_K1162307
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BBa_K844004
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persistent identity: annotation2363082
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BBa_K844004
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BBa_K844004
type: SequenceAnnotation
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BBa_K844004
type: SequenceAnnotation
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BBa_K844004
type: SequenceAnnotation
persistent identity: annotation2363142
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BBa_K844004
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BBa_K844004
type: SequenceAnnotation
persistent identity: annotation2363172
display id: annotation2363172
version: 1
top level: BBa_K1162307
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end: 2033
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BBa_K1162012
type: SequenceAnnotation
persistent identity: annotation2363186
display id: annotation2363186
version: 1
top level: BBa_K1162307
location: https://synbiohub.org/public/igem/BBa_K1162307/annotation2363186/range2363186/1
component: BBa_K1162012

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display id: range2363186
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BBa_K208010
type: ComponentDefinition
persistent identity: BBa K208010
display id: BBa_K208010
version: 1
was generated by: iGEM to SBOL conversion
description: Lac Promoter (BBa_R0010) and RBS (B0034)
created: 2009-10-11T11:00:00Z
modified: 2015-05-08T01:11:24Z
mutable provenance: synthetic
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: lac + rbs
discontinued: false
dominant: false
experience: experience/Works
group u list: _310_
m user id: 0
owner id: 3473
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: none
star: false
creator: Elisabeth Linton
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K208010_sequence/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2034626
display id: annotation2034626
version: 1
top level: BBa_K208010
location: https://synbiohub.org/public/igem/BBa_K208010/annotation2034626/range2034626/1
role: feature/promoter
role: SO:0000167

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type: Range
persistent identity: range2034626
display id: range2034626
version: 1
top level: BBa_K208010
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2034625
display id: annotation2034625
version: 1
top level: BBa_K208010
location: https://synbiohub.org/public/igem/BBa_K208010/annotation2034625/range2034625/1
role: SO:0000139
role: feature/rbs

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type: Range
persistent identity: range2034625
display id: range2034625
version: 1
top level: BBa_K208010
direction: direction/forward
start: 209
end: 220
orientation: inline

BBa_K844004
type: ComponentDefinition
persistent identity: BBa K844004
display id: BBa_K844004
version: 1
was generated by: iGEM to SBOL conversion
description: Spider Silk 1x Subunit "B" (Balanced tRNA codon optimized)
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:33Z
mutable provenance: Artificially designed based on BBa_K844002 and DNA was synthesized
owned by: james
owned by: myers
mutable description: Spider silk subunit optimized to use a more balanced, but still greatly reduced, number of tRNA codons; contains two elasticity domains and one strength domain.
discontinued: false
dominant: false
group u list: _1104_
m user id: 0
owner id: 13876
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: none
star: false
creator: Andrea Halling
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Elasticity Domain
sequence annotation: Beta-helix
sequence annotation: Beta-spiral
sequence annotation: Linker Domain
sequence annotation: Strength Domain
sequence: https://synbiohub.org/public/igem/BBa_K844004_sequence/1

Elasticity Domain
type: SequenceAnnotation
persistent identity: annotation2212319
display id: annotation2212319
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212319/range2212319/1
role: feature/misc
role: SO:0000110

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type: Range
persistent identity: range2212319
display id: range2212319
version: 1
top level: BBa_K844004
direction: direction/forward
start: 1
end: 84
orientation: inline

Beta-helix
type: SequenceAnnotation
persistent identity: annotation2212320
display id: annotation2212320
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212320/range2212320/1
role: SO:0000110
role: feature/misc

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type: Range
persistent identity: range2212320
display id: range2212320
version: 1
top level: BBa_K844004
direction: direction/forward
start: 1
end: 9
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212321
display id: annotation2212321
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212321/range2212321/1
role: SO:0000110
role: feature/misc

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type: Range
persistent identity: range2212321
display id: range2212321
version: 1
top level: BBa_K844004
direction: direction/forward
start: 10
end: 24
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212322
display id: annotation2212322
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212322/range2212322/1
role: SO:0000110
role: feature/misc

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persistent identity: range2212322
display id: range2212322
version: 1
top level: BBa_K844004
direction: direction/forward
start: 31
end: 45
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212323
display id: annotation2212323
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212323/range2212323/1
role: feature/misc
role: SO:0000110

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type: Range
persistent identity: range2212323
display id: range2212323
version: 1
top level: BBa_K844004
direction: direction/forward
start: 46
end: 60
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212324
display id: annotation2212324
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212324/range2212324/1
role: feature/misc
role: SO:0000110

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type: Range
persistent identity: range2212324
display id: range2212324
version: 1
top level: BBa_K844004
direction: direction/forward
start: 69
end: 84
orientation: inline

Elasticity Domain
type: SequenceAnnotation
persistent identity: annotation2212325
display id: annotation2212325
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212325/range2212325/1
role: SO:0000110
role: feature/misc

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persistent identity: range2212325
display id: range2212325
version: 1
top level: BBa_K844004
direction: direction/forward
start: 85
end: 168
orientation: inline

Beta-helix
type: SequenceAnnotation
persistent identity: annotation2212326
display id: annotation2212326
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212326/range2212326/1
role: SO:0000110
role: feature/misc

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type: Range
persistent identity: range2212326
display id: range2212326
version: 1
top level: BBa_K844004
direction: direction/forward
start: 85
end: 94
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212327
display id: annotation2212327
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212327/range2212327/1
role: SO:0000110
role: feature/misc

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type: Range
persistent identity: range2212327
display id: range2212327
version: 1
top level: BBa_K844004
direction: direction/forward
start: 95
end: 110
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212328
display id: annotation2212328
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212328/range2212328/1
role: SO:0000110
role: feature/misc

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type: Range
persistent identity: range2212328
display id: range2212328
version: 1
top level: BBa_K844004
direction: direction/forward
start: 114
end: 129
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212329
display id: annotation2212329
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212329/range2212329/1
role: SO:0000110
role: feature/misc

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type: Range
persistent identity: range2212329
display id: range2212329
version: 1
top level: BBa_K844004
direction: direction/forward
start: 130
end: 144
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212330
display id: annotation2212330
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212330/range2212330/1
role: SO:0000110
role: feature/misc

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type: Range
persistent identity: range2212330
display id: range2212330
version: 1
top level: BBa_K844004
direction: direction/forward
start: 153
end: 168
orientation: inline

Linker Domain
type: SequenceAnnotation
persistent identity: annotation2212331
display id: annotation2212331
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212331/range2212331/1
role: SO:0000110
role: feature/misc

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persistent identity: range2212331
display id: range2212331
version: 1
top level: BBa_K844004
direction: direction/forward
start: 169
end: 185
orientation: inline

Strength Domain
type: SequenceAnnotation
persistent identity: annotation2212332
display id: annotation2212332
version: 1
top level: BBa_K844004
location: https://synbiohub.org/public/igem/BBa_K844004/annotation2212332/range2212332/1
role: SO:0000110
role: feature/misc

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type: Range
persistent identity: range2212332
display id: range2212332
version: 1
top level: BBa_K844004
direction: direction/forward
start: 186
end: 204
orientation: inline

BBa_K1162007
type: ComponentDefinition
persistent identity: BBa K1162007
display id: BBa_K1162007
version: 1
was generated by: iGEM to SBOL conversion
description: WAM-1 antimicrobial peptide from the tammar wallaby (Macropus eugenii)
created: 2013-09-14T11:00:00Z
modified: 2016-01-28T12:14:14Z
mutable provenance: Tammar Wallaby (Macropus eugenii)
owned by: james
owned by: myers
mutable description: An antimicrobial peptide from the Cathelicidin family of peptides, isolated from the Tammar Wallaby (Macropus eugenii). ATG(Met) added at start of coding region.
discontinued: false
dominant: false
experience: experience/None
group u list: _1474_
m user id: 4206
owner id: 13965
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: RFC 23.
star: false
creator: Ryan Putman
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Met
sequence annotation: WAM-1
sequence: https://synbiohub.org/public/igem/BBa_K1162007_sequence/1

Met
type: SequenceAnnotation
persistent identity: annotation2362765
display id: annotation2362765
version: 1
top level: BBa_K1162007
location: https://synbiohub.org/public/igem/BBa_K1162007/annotation2362765/range2362765/1
role: SO:0000318
role: feature/start

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type: Range
persistent identity: range2362765
display id: range2362765
version: 1
top level: BBa_K1162007
direction: direction/forward
start: 1
end: 3
orientation: inline

WAM-1
type: SequenceAnnotation
persistent identity: annotation2362766
display id: annotation2362766
version: 1
top level: BBa_K1162007
location: https://synbiohub.org/public/igem/BBa_K1162007/annotation2362766/range2362766/1
role: SO:0000316
role: feature/cds

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type: Range
persistent identity: range2362766
display id: range2362766
version: 1
top level: BBa_K1162007
direction: direction/forward
start: 4
end: 111
orientation: inline

BBa_K1162012
type: ComponentDefinition
persistent identity: BBa K1162012
display id: BBa_K1162012
version: 1
was generated by: iGEM to SBOL conversion
description: GFP with 10x His Tag
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:32Z
mutable provenance: E. coli.
owned by: james
owned by: myers
mutable description: The GFP present in this part is modified from (BBa_K208000 http://parts.igem.org/wiki/index.php?title=Part:BBa_K208000), with stop codon removed to allow for full translation to 10x His Tag, BBa_K844000 (http://parts.igem.org/wiki/index.php?title=Part:BBa_K844000). This part is useful as it allows the user to carry out gene expression studies and purify the final protein-GFP-His Tag fusion if desired. The prefix in front of the GFP is RFC 23 compatible to allow for in-frame expression of genes. Once protein expression has been demonstrated the gene can be put in front of BBa_K844000 for protein purification without the GFP.
discontinued: false
dominant: false
group u list: _1474_
m user id: 0
owner id: 9404
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: RFC 23 with GFP and 10x His Tag fusion.
star: false
creator: Kathleen Miller
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0015
component: BBa_K844000
component: BBa_K1162011
sequence annotation: BBa_K1162011
sequence annotation: BBa_K844000
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1162012_sequence/1

BBa_B0015
type: Component
persistent identity: component2344037
display id: component2344037
version: 1
top level: BBa_K1162012
definition: BBa_B0015
access: public

BBa_K844000
type: Component
persistent identity: component2344030
display id: component2344030
version: 1
top level: BBa_K1162012
definition: BBa_K844000
access: public

BBa_K1162011
type: Component
persistent identity: component2344026
display id: component2344026
version: 1
top level: BBa_K1162012
definition: BBa_K1162011
access: public

BBa_K1162011
type: SequenceAnnotation
persistent identity: annotation2344026
display id: annotation2344026
version: 1
top level: BBa_K1162012
location: https://synbiohub.org/public/igem/BBa_K1162012/annotation2344026/range2344026/1
component: BBa_K1162011

https://synbiohub.org/public/igem/BBa_K1162012/annotation2344026/range2344026/1
type: Range
persistent identity: range2344026
display id: range2344026
version: 1
top level: BBa_K1162012
direction: direction/reverse
start: 1
end: 717
orientation: inline

BBa_K844000
type: SequenceAnnotation
persistent identity: annotation2344030
display id: annotation2344030
version: 1
top level: BBa_K1162012
location: https://synbiohub.org/public/igem/BBa_K1162012/annotation2344030/range2344030/1
component: BBa_K844000

https://synbiohub.org/public/igem/BBa_K1162012/annotation2344030/range2344030/1
type: Range
persistent identity: range2344030
display id: range2344030
version: 1
top level: BBa_K1162012
direction: direction/reverse
start: 726
end: 761
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2344037
display id: annotation2344037
version: 1
top level: BBa_K1162012
location: https://synbiohub.org/public/igem/BBa_K1162012/annotation2344037/range2344037/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1162012/annotation2344037/range2344037/1
type: Range
persistent identity: range2344037
display id: range2344037
version: 1
top level: BBa_K1162012
direction: direction/reverse
start: 770
end: 898
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K844000
type: ComponentDefinition
persistent identity: BBa K844000
display id: BBa_K844000
version: 1
was generated by: iGEM to SBOL conversion
description: 10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:33Z
mutable provenance: Constructed through oligonucleotide annealing
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: 10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1104_
m user id: 0
owner id: 9404
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: none
star: false
creator: Kathleen Miller
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence annotation: 10x-Histidine Tag
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K844000_sequence/1

10x-Histidine Tag
type: SequenceAnnotation
persistent identity: annotation2206607
display id: annotation2206607
version: 1
top level: BBa_K844000
location: https://synbiohub.org/public/igem/BBa_K844000/annotation2206607/range2206607/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K844000/annotation2206607/range2206607/1
type: Range
persistent identity: range2206607
display id: range2206607
version: 1
top level: BBa_K844000
direction: direction/forward
start: 1
end: 30
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2206608
display id: annotation2206608
version: 1
top level: BBa_K844000
location: https://synbiohub.org/public/igem/BBa_K844000/annotation2206608/range2206608/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K844000/annotation2206608/range2206608/1
type: Range
persistent identity: range2206608
display id: range2206608
version: 1
top level: BBa_K844000
direction: direction/forward
start: 31
end: 33
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2206609
display id: annotation2206609
version: 1
top level: BBa_K844000
location: https://synbiohub.org/public/igem/BBa_K844000/annotation2206609/range2206609/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K844000/annotation2206609/range2206609/1
type: Range
persistent identity: range2206609
display id: range2206609
version: 1
top level: BBa_K844000
direction: direction/forward
start: 34
end: 36
orientation: inline

BBa_K1162011
type: ComponentDefinition
persistent identity: BBa K1162011
display id: BBa_K1162011
version: 1
was generated by: iGEM to SBOL conversion
description: GFP with stop codon removed
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:32Z
mutable provenance: E. coli
owned by: james
owned by: myers
mutable description: Improves upon the original GFP (BBa_K208000) by removing the stop codon. This allows for C terminal addition to this construct. For example purification/secretion tag or a protein fusion with GFP. This GFP confers to RFC 23 silver fusion standard.
discontinued: false
dominant: false
group u list: _1474_
m user id: 0
owner id: 9404
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: RFC 23 standards used. Stop codon from BBa_K208000 removed.
star: false
creator: Kathleen Miller
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1162011_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2343983
display id: annotation2343983
version: 1
top level: BBa_K1162011
location: https://synbiohub.org/public/igem/BBa_K1162011/annotation2343983/range2343983/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K1162011/annotation2343983/range2343983/1
type: Range
persistent identity: range2343983
display id: range2343983
version: 1
top level: BBa_K1162011
direction: direction/forward
start: 1
end: 3
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1162307_sequence/1
type: Sequence
persistent identity: BBa K1162307 sequence
display id: BBa_K1162307_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaacgcggttttggtaaaaaactgcgtaaacgcctgaaaaaattccgcaacagcatcaaaaaacgtctgaaaaactttaacgtggtgattccgattccgctgcctggttactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagcatactagagggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K208010_sequence/1
type: Sequence
persistent identity: BBa K208010 sequence
display id: BBa_K208010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K844004_sequence/1
type: Sequence
persistent identity: BBa K844004 sequence
display id: BBa_K844004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtggatatggtcctggagcaggtcaacaaggaccaggtagtcaaggacctggttctggaggtcaacaaggaccaggtggacaaggtccttatggaccaagtgcagcagcagcagcagca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1162007_sequence/1
type: Sequence
persistent identity: BBa K1162007 sequence
display id: BBa_K1162007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaacgcggttttggtaaaaaactgcgtaaacgcctgaaaaaattccgcaacagcatcaaaaaacgtctgaaaaactttaacgtggtgattccgattccgctgcctggt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1162012_sequence/1
type: Sequence
persistent identity: BBa K1162012 sequence
display id: BBa_K1162012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaatactagagcatcatcaccatcaccaccatcatcaccattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K844000_sequence/1
type: Sequence
persistent identity: BBa K844000 sequence
display id: BBa_K844000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catcatcaccatcaccaccatcatcaccattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1162011_sequence/1
type: Sequence
persistent identity: BBa K1162011 sequence
display id: BBa_K1162011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z