LOCUS       BBa_K1163100             893 bp    DNA     linear       22-Dec-2024
DEFINITION  SuperfolderGFP under the control of the AceB promoter (iron 
            sensitive)
ACCESSION   BBa_K1163100
VERSION     BBa_K1163100.1  
FEATURES             Location/Qualifiers
     RBS             285..294
                     /label=Putative shine-delgarno sequence
     promoter        1..300
                     /label=BBa_K1163400
     CDS             307..756
                     /label=superfolder GFP
     misc_feature    307..756
                     /label=BBa_K1163402
     stem_loop       776..819
                     /label=stem_loop
     misc_feature    765..844
                     /label=BBa_B0010
     terminator      765..844
                     /label=BBa_B0010
     stem_loop       860..879
                     /label=T7 TE
     misc_feature    886..886
                     /label=stop
     polyA_site      880..893
                     /label=polya
     misc_feature    853..893
                     /label=BBa_B0012
     terminator      853..893
                     /label=BBa_B0012
     terminator      765..893
                     /label=BBa_B0015
ORIGIN
        1 atctacggca catgaatcca acgctggatt aatcttctgt gatagtcgat cgttaagcga
       61 ttcagcacct tacctcaggc accttcgggt gcctttttta tttccgaaac gtacctcagc
      121 aggtgaataa attttattca tattgttatc aacaagttat caagtatttt taattaaaat
      181 ggaaattgtt tttgattttg cattttaaat gagtagtctt agttgtgctg aacgaaaaga
      241 gcacaacgat ccttcgttca cagtggggaa gttttcggat ccatgacgag gagctgcacg
      301 tactagatga ctgataacaa taccgcccta aagaaagctg gcctgaaagt aacgcttcct
      361 cgtttaaaaa tcctggaagt tcttcaggag ccggacaacc atcacgtcag tgcggaagat
      421 ttatacaaac gtctgatcga tatgggtgaa gaaattggtc tggctacggt atatcgcgta
      481 ctgaaccagt ttgacgacgc tggtatcgtc acccgccaca attttgaagg cggtaaatcc
      541 gtatttgaac tgacacagca acatcaccac gatcacctga tctgcctcga ctgcggcaag
      601 gttatcgaat ttagtgatga ttccatcgaa gcgcgtcagc gtgaaattgc cgcaaaacat
      661 ggcattcgcc tgactaacca cagtctctat ctttacggtc actgtgccga aggcgattgc
      721 cgcgaagatg aacatgcgca cgaaggcaaa taataatact agagccaggc atcaaataaa
      781 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
      841 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//