BBa_K1163402 1 BBa_K1163402 superfolder GFP 2013-09-21T11:00:00Z 2015-05-08T01:09:32Z DNA synthesis This is a strong superfolder gfp. This part have been characterized fused to the promoter AceB in K1163100, Fes... false false _1475_ 0 14862 9 Not in stock false none false Emiel van der Kouwe annotation2358081 1 superfolder GFP range2358081 1 1 450 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_K1163100 1 BBa_K1163100 SuperfolderGFP under the control of the AceB promoter (iron sensitive) 2013-09-21T11:00:00Z 2015-05-08T01:09:32Z Assembled by goldengate totot false false _1475_ 0 14862 9 Not in stock false none false Emiel van der Kouwe component2358083 1 BBa_K1163400 component2358085 1 BBa_K1163402 component2358092 1 BBa_B0015 annotation2358083 1 BBa_K1163400 range2358083 1 1 300 annotation2358085 1 BBa_K1163402 range2358085 1 307 756 annotation2358092 1 BBa_B0015 range2358092 1 765 893 BBa_K1163400 1 BBa_K1163400 AceB promoter, iron sensitive, down-regulated by dimerized FUR protein 2013-09-21T11:00:00Z 2015-05-08T01:09:32Z Extracted form genomic sequence of E. coli MG1655 Promoter from E. coli that controls the AceB gene, involved in iron uptake. This promoter sequence contains a RBS and a FUR binding site, although it is not clearly possible to identify them precisely. It has been shown to downregulate the expression of sfGFP or any gene down-stream in the presence or iron in the range 10^-7, 10^-5 mol.L-1 false false _1475_ 0 14862 9 Not in stock false Impossible to idengitfy the RBS and FUR binding site precisely false Emiel van der Kouwe annotation2357894 1 Putative shine-delgarno sequence range2357894 1 285 294 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1163402_sequence 1 atgactgataacaataccgccctaaagaaagctggcctgaaagtaacgcttcctcgtttaaaaatcctggaagttcttcaggagccggacaaccatcacgtcagtgcggaagatttatacaaacgtctgatcgatatgggtgaagaaattggtctggctacggtatatcgcgtactgaaccagtttgacgacgctggtatcgtcacccgccacaattttgaaggcggtaaatccgtatttgaactgacacagcaacatcaccacgatcacctgatctgcctcgactgcggcaaggttatcgaatttagtgatgattccatcgaagcgcgtcagcgtgaaattgccgcaaaacatggcattcgcctgactaaccacagtctctatctttacggtcactgtgccgaaggcgattgccgcgaagatgaacatgcgcacgaaggcaaataataa BBa_K1163100_sequence 1 atctacggcacatgaatccaacgctggattaatcttctgtgatagtcgatcgttaagcgattcagcaccttacctcaggcaccttcgggtgccttttttatttccgaaacgtacctcagcaggtgaataaattttattcatattgttatcaacaagttatcaagtatttttaattaaaatggaaattgtttttgattttgcattttaaatgagtagtcttagttgtgctgaacgaaaagagcacaacgatccttcgttcacagtggggaagttttcggatccatgacgaggagctgcacgtactagatgactgataacaataccgccctaaagaaagctggcctgaaagtaacgcttcctcgtttaaaaatcctggaagttcttcaggagccggacaaccatcacgtcagtgcggaagatttatacaaacgtctgatcgatatgggtgaagaaattggtctggctacggtatatcgcgtactgaaccagtttgacgacgctggtatcgtcacccgccacaattttgaaggcggtaaatccgtatttgaactgacacagcaacatcaccacgatcacctgatctgcctcgactgcggcaaggttatcgaatttagtgatgattccatcgaagcgcgtcagcgtgaaattgccgcaaaacatggcattcgcctgactaaccacagtctctatctttacggtcactgtgccgaaggcgattgccgcgaagatgaacatgcgcacgaaggcaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1163400_sequence 1 atctacggcacatgaatccaacgctggattaatcttctgtgatagtcgatcgttaagcgattcagcaccttacctcaggcaccttcgggtgccttttttatttccgaaacgtacctcagcaggtgaataaattttattcatattgttatcaacaagttatcaagtatttttaattaaaatggaaattgtttttgattttgcattttaaatgagtagtcttagttgtgctgaacgaaaagagcacaacgatccttcgttcacagtggggaagttttcggatccatgacgaggagctgcacg BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z