BBa_K1163104
1
BBa_K1163104
FepA promoter region
2013-09-21T11:00:00Z
2015-05-08T01:09:32Z
Genomic sequence
Promoter from E. coli that controls the FepA gene, involved in iron uptake and iron sensitive.
FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In presence of iron, the FUR protein changes its conformation and dimerizes. This leads to the binding to the DNA on a FUR binding site, thus inhibiting the mRNA transcription.<br>
Why would you use this part?<br>
This promoter allows you to have an iron-sensing behaviour. In fact, in the presence of iron, the FepA promoter region decreases the expression of the downstream gene.<br>
false
false
_1475_
0
14862
9
Not in stock
false
We blindly extracted the 320 bp sequence upstream of the FepA gene in E. coli. It contains a putative FUR binding site and a putative RBS.
false
Emiel van der Kouwe
annotation2358138
1
FepA
range2358138
1
1
320
BBa_K1163104_sequence
1
ttcgtcattcagacgctgccattccgggccatgtttcgactgccaccagctctcacttcctacttttaacgccgtcacaccataaccccatgtttactgtgcaatttttcattgattgcagaaatatattgataatattattgataactatttgcatttgcaatagcgtaatggcgcgccgtgggaagcgcggacattaattaaccaactgcactgcgtgtctttcaggatcaaaggttttcgcggtagcgggatgcgtcgtgttgatgacgaccatgcccgacagttgcaattcgtggcaaaaatgcaggaataaaaca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z