BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K1163105 1 BBa_K1163105 FepA-sfGFP-Term 2013-09-21T11:00:00Z 2015-05-08T01:09:32Z Golden Gate assembly between BBa_K1163104 and BBa_B0015. Promoter from E. coli that controls the FepA gene, involved in iron uptake and iron sensitive. FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In presence of iron, the FUR protein changes its conformation and dimerizes. This leads to the binding to the DNA on a FUR binding site, thus inhibiting the mRNA transcription. Why would you use this part? This part allows the characterization of the FepA promoter region thanks to the sfGFP downstream. false false _1475_ 0 14862 9 It's complicated false None. false Emiel van der Kouwe component2358150 1 BBa_K1163002 component2358140 1 BBa_K1163104 annotation2358150 1 BBa_K1163002 range2358150 1 327 1183 annotation2358140 1 BBa_K1163104 range2358140 1 1 320 BBa_K1163104 1 BBa_K1163104 FepA promoter region 2013-09-21T11:00:00Z 2015-05-08T01:09:32Z Genomic sequence Promoter from E. coli that controls the FepA gene, involved in iron uptake and iron sensitive. FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In presence of iron, the FUR protein changes its conformation and dimerizes. This leads to the binding to the DNA on a FUR binding site, thus inhibiting the mRNA transcription.<br> Why would you use this part?<br> This promoter allows you to have an iron-sensing behaviour. In fact, in the presence of iron, the FepA promoter region decreases the expression of the downstream gene.<br> false false _1475_ 0 14862 9 Not in stock false We blindly extracted the 320 bp sequence upstream of the FepA gene in E. coli. It contains a putative FUR binding site and a putative RBS. false Emiel van der Kouwe annotation2358138 1 FepA range2358138 1 1 320 BBa_K1163002 1 BBa_K1163002 sfGFP-Term 2013-09-21T11:00:00Z 2015-05-08T01:09:32Z DNA synthesis Superfolder Green Fluorescent Protein (sfGFP) coding sequence concatenated with a RBS sequence. This part is characterized when coupled to the following promoter regions: AceB in K1163101 FepA in K1163104 Fes in K1163107 YncE in K11631010 false false _1475_ 0 14862 9 Not in stock false None false Emiel van der Kouwe component2358112 1 BBa_K1163001 component2358119 1 BBa_B0015 annotation2358112 1 BBa_K1163001 range2358112 1 1 720 annotation2358119 1 BBa_B0015 range2358119 1 729 857 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_K1163001 1 BBa_K1163001 sfGFP 2013-09-21T11:00:00Z 2015-05-08T01:09:32Z DNA synthesis Superfolder Green Fluorescent Protein (sfGFP) coding sequence. This part is characterized when coupled to the folowing promoter regions: AceB in K1163101 FepA in K1163103 Fes in K1163105 YncE in K1163107 false false _1475_ 0 14862 9 Not in stock false None false Emiel van der Kouwe annotation2358097 1 sfGFP range2358097 1 1 720 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1163105_sequence 1 ttcgtcattcagacgctgccattccgggccatgtttcgactgccaccagctctcacttcctacttttaacgccgtcacaccataaccccatgtttactgtgcaatttttcattgattgcagaaatatattgataatattattgataactatttgcatttgcaatagcgtaatggcgcgccgtgggaagcgcggacattaattaaccaactgcactgcgtgtctttcaggatcaaaggttttcgcggtagcgggatgcgtcgtgttgatgacgaccatgcccgacagttgcaattcgtggcaaaaatgcaggaataaaacatactagatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1163002_sequence 1 atgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1163104_sequence 1 ttcgtcattcagacgctgccattccgggccatgtttcgactgccaccagctctcacttcctacttttaacgccgtcacaccataaccccatgtttactgtgcaatttttcattgattgcagaaatatattgataatattattgataactatttgcatttgcaatagcgtaatggcgcgccgtgggaagcgcggacattaattaaccaactgcactgcgtgtctttcaggatcaaaggttttcgcggtagcgggatgcgtcgtgttgatgacgaccatgcccgacagttgcaattcgtggcaaaaatgcaggaataaaaca BBa_K1163001_sequence 1 atgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaataataa BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z