LOCUS       BBa_K1163112            1571 bp    DNA     linear       22-Dec-2024
DEFINITION  YncE-LacI-LVA-Term
ACCESSION   BBa_K1163112
VERSION     BBa_K1163112.1  
FEATURES             Location/Qualifiers
     promoter        1..300
                     /label=YncE
     promoter        1..300
                     /label=BBa_K1163110
     CDS             307..1434
                     /label=LacI-LVA
     CDS             307..1434
                     /label=BBa_K1163003
     stem_loop       1454..1497
                     /label=stem_loop
     misc_feature    1443..1522
                     /label=BBa_B0010
     terminator      1443..1522
                     /label=BBa_B0010
     stem_loop       1538..1557
                     /label=T7 TE
     misc_feature    1564..1564
                     /label=stop
     polyA_site      1558..1571
                     /label=polya
     misc_feature    1531..1571
                     /label=BBa_B0012
     terminator      1531..1571
                     /label=BBa_B0012
     terminator      1443..1571
                     /label=BBa_B0015
     misc_feature    307..1571
                     /label=BBa_K1163004
ORIGIN
        1 acgggggaaa ggacaagcag tgcgggcaaa acggtctgtc ggacggaaaa aatcttcatg
       61 aaaaaagcca ggttaagaat gggaaaacgc cgtcatggta atgaaattgt aaatttatgg
      121 aaaatgaaac ggcacaatac gttaagtaat tgagaaaatt gtagtcgtaa cggcaagaaa
      181 tgctccacat ttgagaaaat aatgattacc attcccattt ataacaagag cgtaacgatg
      241 attacgctta gcgaagcatt gtgaagcagc aaaaatatcg gttcatcaaa gggagtcgtc
      301 tactagatgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc
      361 tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg
      421 gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa
      481 ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg
      541 ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg
      601 gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc
      661 gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct
      721 gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc
      781 atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc
      841 gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt
      901 ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa
      961 cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag
     1021 ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc
     1081 gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat
     1141 accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg
     1201 ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc
     1261 aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa
     1321 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     1381 ctggaaagcg ggcaggctgc aaacgacgaa aactacgctt tagtagctta ataatactag
     1441 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1501 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1561 ctgcgtttat a
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