BBa_K1164002 1 BBa_K1164002 LacI repressor tagged with yBFP 2013-09-15T11:00:00Z 2015-05-08T01:09:32Z E.coli/commercial synthesis This is the LacI repressor from E.coli tagged with yeast codon optimized BFP. LacI will bind with high specificity to the the operator lacO and is inhibited in the presence of IPTG. BFP is a monomeric fluorescent protein that has an excitation peak of 399 nm and an emission peak of 465 nm. It was yeast codon optimized through DNA synthesis for the purpose of expressing in S. cerevisiae tagged to various repressors and activators. This is an updated version of BBa_K642001 that is functional in S. cerevisiae. false false _1476_ 0 10002 9 It's complicated false In order to remove the illegal XbaI site, site-directed mutagenesis was performed, changing nucleotide 255 from a thymine to a cytosine, and also changing nucleotide 258 from an adenine to a glycine. false Wilson Lam, Dylan Siriwardena annotation2346173 1 lacI range2346173 1 1 1080 annotation2346174 1 yBFP range2346174 1 1081 1782 BBa_K1164002_sequence 1 atgaaaccagtaacattgtatgatgtcgcagagtatgccggtgtctcttatcagactgtttccagagtggtgaaccaggccagccatgtttctgccaaaaccagggaaaaagtggaagcagccatggcagagctgaattacattcccaacagagtggcacaacaactggcaggcaaacagagcttgctgattggagttgccacctccagtctggccctgcatgcaccatctcaaattgtggcagccattaaatccagggctgatcaactgggagcctctgtggtggtgtcaatggtagaaagaagtggagttgaagcctgtaaagctgctgtgcacaatcttctggcacaaagagtcagtgggctgatcattaactatccactggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccagcactctttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagatggtacaagactgggtgtggagcatctggttgcattgggacaccagcaaattgcactgcttgcgggcccactcagttctgtctcagcaaggctgagactggccggctggcataaatatctcactaggaatcaaattcagccaatagctgaaagagaaggggactggagtgccatgtctgggtttcaacaaaccatgcaaatgctgaatgagggcattgttcccactgcaatgctggttgccaatgatcagatggcactgggtgcaatgagagccattactgagtctgggctgagagttggtgcagatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgccagtctcactggtgaagagaaaaaccaccctggcacccaatacacaaactgcctctccccgggcattggctgattcactcatgcagctagcaagacaggtttccagactggaaagtgggcagatgtccgaagaattgatcaaggaaaacatgcacatgaaattgtatatggaaggtactgtcgacaaccaccacttcaaatgcacctccgaaggtgaaggtaaaccttatgaaggtacacaaaccatgagaatcaaagtcgtcgaaggtggtccattgccatttgctttcgacattttggccacatctttcttgtatggttccaaaactttcatcaatcacacccaaggtattccagacttctttaaacaatctttccctgaaggtttcacttgggaaagagtcaccacctatgaagatggtggtgtcttgactgctactcaagacacatccttacaagacggttgcttgatctataacgtcaagattagaggtgtcaacttcacatcaaacggtcctgtcatgcaaaaaaagacattgggttgggaagctttcaccgaaactttgtatcctgccgacggtggtttagaaggtagaaacgacatggccttaaaattggtcggtggtagtcacttgattgccaacatcaaaacaacctatagatccaaaaaacctgccaaaaacttgaaaatgcctggtgtctattatgtcgactatagattggaaagaattaaggaagccaacaacgaaacttatgtcgaacaacacgaagttgctgtcgccagatattgtgacttgccttcaaaattgggtcacaaattgaac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z