BBa_K1164008 1 BBa_K1164008 pTRELX driving yeGFP with tPGK1 terminator 2013-09-15T11:00:00Z 2015-05-08T01:09:32Z S.cerevisiae/commercial synthesis This device consists of a modified version of the native Gal1 promoter found in S.cerevisiae driving production of yeast codon optimized GFP. The four gal4 binding domains present in the native Gal1 promoter have been replaced by four tetO sites. This promoter may be activated by regulatory activators that are able to bind to tetO. In addition, two lacO operators have been introduced downstream of the TATA box, allowing for repression of gene expression by lacI. Upon expression from the promoter, yEGFP will be produced. false false _1476_ 0 10002 9 It's complicated false N/A false Wilson Lam, Dylan Siriwardena, Lloyd Mai, Yara Abou-Hamde annotation2346192 1 tetO range2346192 1 47 65 annotation2346197 1 tPGK1 range2346197 1 1273 1466 annotation2346196 1 yEGFP range2346196 1 559 1272 annotation2346195 1 pTRELX range2346195 1 1 558 annotation2346191 1 tetO range2346191 1 28 46 annotation2346190 1 tetO range2346190 1 7 25 annotation2346193 1 tetO range2346193 1 113 131 annotation2346194 1 lacO range2346194 1 352 368 BBa_K1164008_sequence 1 gaagtatccctatcagtgatagagaagtccctatcagtgatagagatccctatcagtgatagagatgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgccttccctatcagtgatagagaaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaattaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtactccaacagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgttgttgtgtggaattgtgagcggataacaatttcacacaatattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggagaaaaaccccggatccacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggagaaaaaccccggatccatgtctaaaggtgaagaattattcactggtgttgtcccaattttggttgaattagatggtgatgttaatggtcacaaattttctgtctccggtgaaggtgaaggtgatgctacttacggtaaattgaccttaaaatttatttgtactactggtaaattgccagttccatggccaaccttagtcactactttcggttatggtgttcaatgttttgctagatacccagatcatatgaaacaacatgactttttcaagtctgccatgccagaaggttatgttcaagaaagaactatttttttcaaagatgacggtaactacaagaccagagctgaagtcaagtttgaaggtgataccttagttaatagaatcgaattaaaaggtattgattttaaagaagatggtaacattttaggtcacaaattggaatacaactataactctcacaatgtttacatcatggctgacaaacaaaagaatggtatcaaagttaacttcaaaattagacacaacattgaagatggttctgttcaattagctgaccattatcaacaaaatactccaattggtgatggtccagtcttgttaccagacaaccattacttatccactcaatctgccttatccaaagatccaaacgaaaagagagaccacatggtcttgttagaatttgttactgctgctggtattacccatggtatggatgaattgtacaaataaattgaattgaattgaaatcgatagatcaatttttttcttttctctttccccatcctttacgctaaaataatagtttattttattttttgaatattttttatttatatacgtatatatagactattatttatcttttaatgattattaagatttttattaaaaaaaaattcgctcctcttttaatgccttta igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z