##gff-version 3
##sequence-region BBa_K1165014 1 1018
BBa_K1165014	.	promoter	1	108	.	+	0	ID=BBa_R0082;Name=BBa_R0082
BBa_K1165014	.	non_covalent_binding_site	54	71	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_K1165014	.	non_covalent_binding_site	13	30	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_K1165014	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_K1165014	.	promoter	98	103	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_K1165014	.	promoter	75	80	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_K1165014	.	engineered_region	117	1018	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400
BBa_K1165014	.	terminator	828	907	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_K1165014	.	stem_loop	839	882	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1165014	.	engineered_region	828	907	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1165014	.	terminator	916	956	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_K1165014	.	polyA_site	943	956	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1165014	.	stem_loop	923	942	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1165014	.	stop_codon	949	949	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1165014	.	engineered_region	916	956	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1165014	.	ribosome_entry_site	117	128	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_K1165014	.	conserved_region	121	124	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1165014	.	promoter	965	1018	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_K1165014	.	promoter	1007	1012	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1165014	.	non_covalent_binding_site	965	983	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1165014	.	engineered_region	965	1018	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1165014	.	promoter	984	989	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1165014	.	non_covalent_binding_site	990	1008	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1165014	.	CDS	135	794	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_K1165014	.	CDS	138	754	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1165014	.	tag	755	788	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1165014	.	engineered_tag	795	819	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
>BBa_K1165014
tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttgga
ttattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatgtccagattagataaaagtaaa
gtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctag
gtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttaga
taggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttaga
tgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcg
aaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattt
tactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatg
ccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattga
tcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagctta
ataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac