LOCUS BBa_K116504 3420 bp DNA linear 16-Dec-2024 DEFINITION Tet+RBS+KaiA+Ter+Lac+RBS+KaiB+Lac+RBS+KaiC ACCESSION BBa_K116504 VERSION BBa_K116504.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 83..940 /label=BBa_J36801 stem_loop 960..1003 /label=stem_loop misc_feature 949..1028 /label=BBa_B0010 terminator 949..1028 /label=BBa_B0010 stem_loop 1044..1063 /label=T7 TE misc_feature 1070..1070 /label=stop polyA_site 1064..1077 /label=polya misc_feature 1037..1077 /label=BBa_B0012 terminator 1037..1077 /label=BBa_B0012 CDS 1086..1173 /label=end of LacI coding region (inactive) misc_feature 1086..1285 /label=BBa_R0010 misc_feature 1174..1211 /label=CAP binding site promoter 1222..1227 /label=-35 promoter 1246..1251 /label=-10 misc_feature 1251..1285 /label=LacI binding site misc_feature 1258..1258 /label=start promoter 1086..1285 /label=BBa_R0010 misc_feature 1298..1301 /label=conserved RBS 1294..1305 /label=BBa_B0034 CDS 1312..1623 /label=BBa_J36802 CDS 1632..1719 /label=end of LacI coding region (inactive) misc_feature 1632..1831 /label=BBa_R0010 misc_feature 1720..1757 /label=CAP binding site promoter 1768..1773 /label=-35 promoter 1792..1797 /label=-10 misc_feature 1797..1831 /label=LacI binding site misc_feature 1804..1804 /label=start promoter 1632..1831 /label=BBa_R0010 misc_feature 1844..1847 /label=conserved RBS 1840..1851 /label=BBa_B0034 CDS 1858..3420 /label=BBa_J36804 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag aggtgctctc gcaaattgca atctgcattt gggtggaatc 121 gacggcaatt ttgcaggatt gccagcgggc gctgtcggcc gatcgctatc aactccaagt 181 ctgtgagtct ggcgaaatgc tcttggagta tgcccaaacc catcgtgacc aaatcgactg 241 cctgatttta gtggcagcca atcccagctt cagggcagtt gttcagcagc tctgctttga 301 gggagtggtg gtaccagcga ttgtcgtagg cgatcgcgac agtgaggatc ccgatgaacc 361 agccaaagaa cagctctatc acagcgctga actgcacctc ggtatccatc agctcgagca 421 attgccctac caagttgatg ctgcactggc tgaatttctg cgcttagccc cggtcgagac 481 catggccgac cacatcatgc tgatgggggc caaccacgat cccgagctat cgagccagca 541 gcgggacctc gctcagcgac tacaagagcg cctaggctat ctcggggtct actacaagcg 601 tgatcccgat cgctttctgc gcaacctacc cgcctacgaa agccaaaagc tgcaccaagc 661 gatgcagact agctatcgtg aaatcgtttt gagctatttt tcgccgaata gcaacctcaa 721 ccagagcatt gacaacttcg tcaacatggc tttctttgcc gatgttccag tcaccaaagt 781 ggtagaaatt cacatggagc tgatggacga gtttgccaag aagctccgcg tagagggacg 841 ttcagaggac attttgctgg attatcggct gactttaatt gatgtaattg cacatctttg 901 tgagatgtat cgacggtcta tcccacgaga aacctgatga tactagagcc aggcatcaaa 961 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 1021 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac 1081 tagagcaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 1141 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 1201 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 1261 tgtgagcgga taacaatttc acacatacta gagaaagagg agaaatacta gatgagccct 1321 cgtaaaacct acattctcaa gctctacgtc gccggcaata ctccaaactc agtccgtgcc 1381 ctcaaaacgc tcaagaacat tctcgaagtt gaatttcaag gtgtttatgc tctaaaggtg 1441 atcgatgttc tcaaaaatcc tcagttggca gaagaggata aaatcctagc gacgccaacc 1501 ctcgccaagg ttctaccact gcctgtccga cggattattg gtgatttatc cgaccgtgag 1561 aaagttttga ttggccttga tttactctac ggcgaacttc aagattccga cgacttctaa 1621 taatactaga gcaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 1681 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 1741 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 1801 tggaattgtg agcggataac aatttcacac atactagaga aagaggagaa atactagatg 1861 acttccgctg agatgactag ccctaataat aattctgagc accaagccat cgctaagatg 1921 cgcacgatga ttgaaggctt tgatgatatt agtcatggcg gtcttccaat cgggcgatcg 1981 accctcgtta gtggtacttc aggaaccggc aagacccttt tttctattca atttctctat 2041 aacggtatta tcgagtttga tgagcctggg gttttcgtta ctttcgaaga aaccccgcaa 2101 gatatcatta aaaacgcccg tagttttggc tgggatttag ccaagctggt cgatgagggc 2161 aaactattta ttcttgatgc ttcacccgat ccagaaggtc aagaggttgt tggcggcttc 2221 gatctctctg ctctgattga gcggattaat tatgcaattc aaaagtatcg agcgcggcgg 2281 gtttcaattg actcggtcac gtccgttttc cagcaatatg atgcctcttc tgtggttcgc 2341 cgcgaactct ttcggttggt agctcgccta aaacaaattg gggcaactac ggtcatgacc 2401 accgagcgta tcgaggaata tggcccgatc gctcgttacg gtgttgagga atttgtctcc 2461 gataacgtcg tgattctccg caacgttttg gaaggggagc gccgtcgccg caccctcgaa 2521 atcctcaagc tacgtggcac cagccacatg aaaggggaat atccgttcac gattacggat 2581 catggcatca atatcttccc gctcggggca atgcgcctta cgcagcgatc gtcgaacgtg 2641 cgtgtttcat ctggtgtcgt ccgactcgat gaaatgtgtg gtgggggctt ctttaaggac 2701 tcaatcattc tggcaactgg cgctacaggc actggtaaaa ctctgttagt tagccgtttc 2761 gttgagaatg cttgtgctaa caaagagcgg gcgattctgt tcgcttatga agagtcacga 2821 gctcagctgc tccgcaatgc ctattcatgg ggaatggact ttgaggagat ggagcgccaa 2881 aacctcctca aaattgtttg cgcctatcct gaatccgcag gtcttgaaga ccatttgcag 2941 attattaagt cggagatcaa tgactttaag ccagctcgta ttgcaatcga ctccctctct 3001 gctttggcgc ggggcgttag caacaatgcc ttccgccaat ttgtaattgg tgtcactggc 3061 tacgcgaaac aagaagaaat cacgggacta ttcacaaata ccagtgatca atttatggga 3121 gcgcattcga ttactgactc ccatatctca acaattacgg atacgattat cttgctccaa 3181 tacgtcgaga ttcgtggcga aatgtcccgc gccattaacg tcttcaagat gcgcggatct 3241 tggcatgaca aagcaatccg cgagttcatg atcagcgaca aagggccgga catcaaggat 3301 tctttccgga actttgagcg gattatttca ggttcgccaa cacggattac cgtcgatgag 3361 aaaagcgaac tctcgcgaat tgtgcgcggc gttcaagaaa aagggccgga gagctagtag //