##gff-version 3
##sequence-region BBa_K116504 1 3420
BBa_K116504	.	terminator	1037	1077	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K116504	.	polyA_site	1064	1077	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116504	.	stem_loop	1044	1063	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116504	.	stop_codon	1070	1070	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116504	.	engineered_region	1037	1077	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116504	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K116504	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K116504	.	promoter	1086	1285	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K116504	.	promoter	1246	1251	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K116504	.	CDS	1086	1173	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K116504	.	promoter	1222	1227	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K116504	.	non_covalent_binding_site	1174	1211	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K116504	.	start_codon	1258	1258	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K116504	.	non_covalent_binding_site	1251	1285	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K116504	.	engineered_region	1086	1285	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K116504	.	terminator	949	1028	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K116504	.	stem_loop	960	1003	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116504	.	engineered_region	949	1028	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116504	.	ribosome_entry_site	1294	1305	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K116504	.	conserved_region	1298	1301	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K116504	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K116504	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K116504	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K116504	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K116504	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K116504	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K116504	.	CDS	1312	1623	.	+	0	ID=BBa_J36802;Name=BBa_J36802
BBa_K116504	.	promoter	1632	1831	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K116504	.	promoter	1792	1797	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K116504	.	CDS	1632	1719	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K116504	.	promoter	1768	1773	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K116504	.	non_covalent_binding_site	1720	1757	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K116504	.	start_codon	1804	1804	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K116504	.	non_covalent_binding_site	1797	1831	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K116504	.	engineered_region	1632	1831	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K116504	.	ribosome_entry_site	1840	1851	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K116504	.	conserved_region	1844	1847	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K116504	.	CDS	83	940	.	+	0	ID=BBa_J36801;Name=BBa_J36801
BBa_K116504	.	CDS	1858	3420	.	+	0	ID=BBa_J36804;Name=BBa_J36804
>BBa_K116504
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gaggtgctctcgcaaattgcaatctgcatttgggtggaatcgacggcaattttgcaggattgccagcgggcgctgtcgg
ccgatcgctatcaactccaagtctgtgagtctggcgaaatgctcttggagtatgcccaaacccatcgtgaccaaatcga
ctgcctgattttagtggcagccaatcccagcttcagggcagttgttcagcagctctgctttgagggagtggtggtacca
gcgattgtcgtaggcgatcgcgacagtgaggatcccgatgaaccagccaaagaacagctctatcacagcgctgaactgc
acctcggtatccatcagctcgagcaattgccctaccaagttgatgctgcactggctgaatttctgcgcttagccccggt
cgagaccatggccgaccacatcatgctgatgggggccaaccacgatcccgagctatcgagccagcagcgggacctcgct
cagcgactacaagagcgcctaggctatctcggggtctactacaagcgtgatcccgatcgctttctgcgcaacctacccg
cctacgaaagccaaaagctgcaccaagcgatgcagactagctatcgtgaaatcgttttgagctatttttcgccgaatag
caacctcaaccagagcattgacaacttcgtcaacatggctttctttgccgatgttccagtcaccaaagtggtagaaatt
cacatggagctgatggacgagtttgccaagaagctccgcgtagagggacgttcagaggacattttgctggattatcggc
tgactttaattgatgtaattgcacatctttgtgagatgtatcgacggtctatcccacgagaaacctgatgatactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctcc
ccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaat
taatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtg
agcggataacaatttcacacatactagagaaagaggagaaatactagatgagccctcgtaaaacctacattctcaagct
ctacgtcgccggcaatactccaaactcagtccgtgccctcaaaacgctcaagaacattctcgaagttgaatttcaaggt
gtttatgctctaaaggtgatcgatgttctcaaaaatcctcagttggcagaagaggataaaatcctagcgacgccaaccc
tcgccaaggttctaccactgcctgtccgacggattattggtgatttatccgaccgtgagaaagttttgattggccttga
tttactctacggcgaacttcaagattccgacgacttctaataatactagagcaatacgcaaaccgcctctccccgcgcg
ttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtg
agttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggat
aacaatttcacacatactagagaaagaggagaaatactagatgacttccgctgagatgactagccctaataataattct
gagcaccaagccatcgctaagatgcgcacgatgattgaaggctttgatgatattagtcatggcggtcttccaatcgggc
gatcgaccctcgttagtggtacttcaggaaccggcaagacccttttttctattcaatttctctataacggtattatcga
gtttgatgagcctggggttttcgttactttcgaagaaaccccgcaagatatcattaaaaacgcccgtagttttggctgg
gatttagccaagctggtcgatgagggcaaactatttattcttgatgcttcacccgatccagaaggtcaagaggttgttg
gcggcttcgatctctctgctctgattgagcggattaattatgcaattcaaaagtatcgagcgcggcgggtttcaattga
ctcggtcacgtccgttttccagcaatatgatgcctcttctgtggttcgccgcgaactctttcggttggtagctcgccta
aaacaaattggggcaactacggtcatgaccaccgagcgtatcgaggaatatggcccgatcgctcgttacggtgttgagg
aatttgtctccgataacgtcgtgattctccgcaacgttttggaaggggagcgccgtcgccgcaccctcgaaatcctcaa
gctacgtggcaccagccacatgaaaggggaatatccgttcacgattacggatcatggcatcaatatcttcccgctcggg
gcaatgcgccttacgcagcgatcgtcgaacgtgcgtgtttcatctggtgtcgtccgactcgatgaaatgtgtggtgggg
gcttctttaaggactcaatcattctggcaactggcgctacaggcactggtaaaactctgttagttagccgtttcgttga
gaatgcttgtgctaacaaagagcgggcgattctgttcgcttatgaagagtcacgagctcagctgctccgcaatgcctat
tcatggggaatggactttgaggagatggagcgccaaaacctcctcaaaattgtttgcgcctatcctgaatccgcaggtc
ttgaagaccatttgcagattattaagtcggagatcaatgactttaagccagctcgtattgcaatcgactccctctctgc
tttggcgcggggcgttagcaacaatgccttccgccaatttgtaattggtgtcactggctacgcgaaacaagaagaaatc
acgggactattcacaaataccagtgatcaatttatgggagcgcattcgattactgactcccatatctcaacaattacgg
atacgattatcttgctccaatacgtcgagattcgtggcgaaatgtcccgcgccattaacgtcttcaagatgcgcggatc
ttggcatgacaaagcaatccgcgagttcatgatcagcgacaaagggccggacatcaaggattctttccggaactttgag
cggattatttcaggttcgccaacacggattaccgtcgatgagaaaagcgaactctcgcgaattgtgcgcggcgttcaag
aaaaagggccggagagctagtag