LOCUS BBa_K116510 1010 bp DNA linear 22-Nov-2024 DEFINITION GFP under control of pOmpf promoter(K116500+E0240) ACCESSION BBa_K116510 VERSION BBa_K116510.1 FEATURES Location/Qualifiers misc_feature 27..88 /label=OmpR binding promoter 1..126 /label=BBa_K116500 RBS 135..147 /label=RBS-3\Weak RBS 141..144 /label=RBS misc_feature 135..147 /label=BBa_B0032 RBS 135..147 /label=BBa_B0032 CDS 154..873 /label=GFP protein CDS 154..873 /label=BBa_E0040 stem_loop 893..936 /label=stem_loop misc_feature 882..961 /label=BBa_B0010 terminator 882..961 /label=BBa_B0010 stem_loop 977..996 /label=T7 TE misc_feature 1003..1003 /label=stop polyA_site 997..1010 /label=polya misc_feature 970..1010 /label=BBa_B0012 terminator 970..1010 /label=BBa_B0012 ORIGIN 1 gacggtgttc acaaagttcc ttaaatttta cttttggtta catatttttt ctttttgaaa 61 ccaaatcttt atctttgtag cactttcacg gtagcgaaac gttagtttga atggaaagat 121 gcctgctact agagtcacac aggaaagtac tagatgcgta aaggagaaga acttttcact 181 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc 241 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact 301 actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa 361 tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc 421 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt 481 gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat 541 tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat 601 gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac 661 aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc 721 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa 781 gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt 841 acacatggca tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg 901 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 961 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //