LOCUS BBa_K116514 4747 bp DNA linear 08-Dec-2025 DEFINITION KaiABC with SasA output ACCESSION BBa_K116514 VERSION BBa_K116514.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..1182 /label=BBa_K116502 stem_loop 1202..1245 /label=stem_loop misc_feature 1191..1270 /label=BBa_B0010 terminator 1191..1270 /label=BBa_B0010 stem_loop 1286..1305 /label=T7 TE misc_feature 1312..1312 /label=stop polyA_site 1306..1319 /label=polya misc_feature 1279..1319 /label=BBa_B0012 terminator 1279..1319 /label=BBa_B0012 misc_feature 1328..1346 /label=TetR 1 misc_feature 1328..1381 /label=BBa_R0040 promoter 1347..1352 /label=-35 misc_feature 1353..1371 /label=TetR 2 promoter 1370..1375 /label=-10 promoter 1328..1381 /label=BBa_R0040 misc_feature 1394..1397 /label=conserved RBS 1390..1401 /label=BBa_B0034 CDS 1410..2267 /label=BBa_J36801 stem_loop 2287..2330 /label=stem_loop misc_feature 2276..2355 /label=BBa_B0010 terminator 2276..2355 /label=BBa_B0010 stem_loop 2371..2390 /label=T7 TE misc_feature 2397..2397 /label=stop polyA_site 2391..2404 /label=polya misc_feature 2364..2404 /label=BBa_B0012 terminator 2364..2404 /label=BBa_B0012 CDS 2413..2500 /label=end of LacI coding region (inactive) misc_feature 2413..2612 /label=BBa_R0010 misc_feature 2501..2538 /label=CAP binding site promoter 2549..2554 /label=-35 promoter 2573..2578 /label=-10 misc_feature 2578..2612 /label=LacI binding site misc_feature 2585..2585 /label=start promoter 2413..2612 /label=BBa_R0010 misc_feature 2625..2628 /label=conserved RBS 2621..2632 /label=BBa_B0034 CDS 2639..2950 /label=BBa_J36802 CDS 2959..3046 /label=end of LacI coding region (inactive) misc_feature 2959..3158 /label=BBa_R0010 misc_feature 3047..3084 /label=CAP binding site promoter 3095..3100 /label=-35 promoter 3119..3124 /label=-10 misc_feature 3124..3158 /label=LacI binding site misc_feature 3131..3131 /label=start promoter 2959..3158 /label=BBa_R0010 misc_feature 3171..3174 /label=conserved RBS 3167..3178 /label=BBa_B0034 CDS 3185..4747 /label=BBa_J36804 ORIGIN 1 aaagaggaga aatactagat gggagagtct ctgtcaccac aagcactggc tcaaccgttg 61 ctactgcaac tgttcgtcga tacccggccc ctgtcacagc acattgtgca gcgggttaaa 121 aatattttgg cagcagtaga ggcaaccgtc cccatcagct tgcaggtgat caatgtggcg 181 gaccagccac aactggtgga gtactaccgc ctagtcgtca cgcctgccct ggttaaaatt 241 ggtccaggct ctcgccaagt tctgagtggc atcgacctca ccgatcaatt agccaaccag 301 ttgccccagt ggctggttca gcaagaggcc ttttttgccg atcgagagcc acctgaagtc 361 aacattccgt tcacggagct aggccaaccc gagacccccg cgttgcagca ggctgatgcc 421 ttttttcagc ttcagcaaca atacgctgat ctctcggagc ggacaaaatt ccttgagcag 481 gtcattgctc tcgtcgccca cgacctccgc aatccgctaa ccgctgctct tttggcggtc 541 gacaccattc aaatccgcag tcaatccttt tctgtggcga cagccaagga aatgcaggga 601 ctgtgcagtc tgttcgatca ggcacgatcg caattgcgtg aaatcgagcg catgattgcc 661 gagattttgg aggcaactcg ccactctggc gaaagcttgc ggatcaatcc ccgcgaagtc 721 gtctttgagc cgctcttaca acaggttctg gaacagttgc atgaacgctg gcggagtaag 781 cagcagcagt tgatcacgga tgttccaggc gacctgccca cgctctacgc cgaccctgac 841 cgtctgcggc aggttctggt caatctgcta gacaacgcca tcaaatacac tccgcccggg 901 ggcacaatca cgatcgcagc gctccatcgc acgagccaga aagtgcagat cagcatcagc 961 gacacgggct ctggcatccc tcgcgaccag ctcagcgtca ttttcaagaa cttggtgcgg 1021 ctttcccgcg atagcagcca agagggctac ggcattggac tatcggtttg ccagcgaatt 1081 gttcaggccc actttggccg catctgggtt gcttcggaac tgggccaagg cagcaccttc 1141 cacttcacga tgccggttta tcgctacacc atgccctgct aatactagag ccaggcatca 1201 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 1261 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat 1321 actagagtcc ctatcagtga tagagattga catccctatc agtgatagag atactgagca 1381 ctactagaga aagaggagaa atactagagg tgctctcgca aattgcaatc tgcatttggg 1441 tggaatcgac ggcaattttg caggattgcc agcgggcgct gtcggccgat cgctatcaac 1501 tccaagtctg tgagtctggc gaaatgctct tggagtatgc ccaaacccat cgtgaccaaa 1561 tcgactgcct gattttagtg gcagccaatc ccagcttcag ggcagttgtt cagcagctct 1621 gctttgaggg agtggtggta ccagcgattg tcgtaggcga tcgcgacagt gaggatcccg 1681 atgaaccagc caaagaacag ctctatcaca gcgctgaact gcacctcggt atccatcagc 1741 tcgagcaatt gccctaccaa gttgatgctg cactggctga atttctgcgc ttagccccgg 1801 tcgagaccat ggccgaccac atcatgctga tgggggccaa ccacgatccc gagctatcga 1861 gccagcagcg ggacctcgct cagcgactac aagagcgcct aggctatctc ggggtctact 1921 acaagcgtga tcccgatcgc tttctgcgca acctacccgc ctacgaaagc caaaagctgc 1981 accaagcgat gcagactagc tatcgtgaaa tcgttttgag ctatttttcg ccgaatagca 2041 acctcaacca gagcattgac aacttcgtca acatggcttt ctttgccgat gttccagtca 2101 ccaaagtggt agaaattcac atggagctga tggacgagtt tgccaagaag ctccgcgtag 2161 agggacgttc agaggacatt ttgctggatt atcggctgac tttaattgat gtaattgcac 2221 atctttgtga gatgtatcga cggtctatcc cacgagaaac ctgatgatac tagagccagg 2281 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 2341 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 2401 tatatactag agcaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 2461 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 2521 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt 2581 gtggaattgt gagcggataa caatttcaca catactagag aaagaggaga aatactagat 2641 gagccctcgt aaaacctaca ttctcaagct ctacgtcgcc ggcaatactc caaactcagt 2701 ccgtgccctc aaaacgctca agaacattct cgaagttgaa tttcaaggtg tttatgctct 2761 aaaggtgatc gatgttctca aaaatcctca gttggcagaa gaggataaaa tcctagcgac 2821 gccaaccctc gccaaggttc taccactgcc tgtccgacgg attattggtg atttatccga 2881 ccgtgagaaa gttttgattg gccttgattt actctacggc gaacttcaag attccgacga 2941 cttctaataa tactagagca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 3001 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 3061 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 3121 tgttgtgtgg aattgtgagc ggataacaat ttcacacata ctagagaaag aggagaaata 3181 ctagatgact tccgctgaga tgactagccc taataataat tctgagcacc aagccatcgc 3241 taagatgcgc acgatgattg aaggctttga tgatattagt catggcggtc ttccaatcgg 3301 gcgatcgacc ctcgttagtg gtacttcagg aaccggcaag accctttttt ctattcaatt 3361 tctctataac ggtattatcg agtttgatga gcctggggtt ttcgttactt tcgaagaaac 3421 cccgcaagat atcattaaaa acgcccgtag ttttggctgg gatttagcca agctggtcga 3481 tgagggcaaa ctatttattc ttgatgcttc acccgatcca gaaggtcaag aggttgttgg 3541 cggcttcgat ctctctgctc tgattgagcg gattaattat gcaattcaaa agtatcgagc 3601 gcggcgggtt tcaattgact cggtcacgtc cgttttccag caatatgatg cctcttctgt 3661 ggttcgccgc gaactctttc ggttggtagc tcgcctaaaa caaattgggg caactacggt 3721 catgaccacc gagcgtatcg aggaatatgg cccgatcgct cgttacggtg ttgaggaatt 3781 tgtctccgat aacgtcgtga ttctccgcaa cgttttggaa ggggagcgcc gtcgccgcac 3841 cctcgaaatc ctcaagctac gtggcaccag ccacatgaaa ggggaatatc cgttcacgat 3901 tacggatcat ggcatcaata tcttcccgct cggggcaatg cgccttacgc agcgatcgtc 3961 gaacgtgcgt gtttcatctg gtgtcgtccg actcgatgaa atgtgtggtg ggggcttctt 4021 taaggactca atcattctgg caactggcgc tacaggcact ggtaaaactc tgttagttag 4081 ccgtttcgtt gagaatgctt gtgctaacaa agagcgggcg attctgttcg cttatgaaga 4141 gtcacgagct cagctgctcc gcaatgccta ttcatgggga atggactttg aggagatgga 4201 gcgccaaaac ctcctcaaaa ttgtttgcgc ctatcctgaa tccgcaggtc ttgaagacca 4261 tttgcagatt attaagtcgg agatcaatga ctttaagcca gctcgtattg caatcgactc 4321 cctctctgct ttggcgcggg gcgttagcaa caatgccttc cgccaatttg taattggtgt 4381 cactggctac gcgaaacaag aagaaatcac gggactattc acaaatacca gtgatcaatt 4441 tatgggagcg cattcgatta ctgactccca tatctcaaca attacggata cgattatctt 4501 gctccaatac gtcgagattc gtggcgaaat gtcccgcgcc attaacgtct tcaagatgcg 4561 cggatcttgg catgacaaag caatccgcga gttcatgatc agcgacaaag ggccggacat 4621 caaggattct ttccggaact ttgagcggat tatttcaggt tcgccaacac ggattaccgt 4681 cgatgagaaa agcgaactct cgcgaattgt gcgcggcgtt caagaaaaag ggccggagag 4741 ctagtag //