##gff-version 3
##sequence-region BBa_K116514 1 4747
BBa_K116514	.	promoter	2959	3158	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K116514	.	engineered_region	2959	3158	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K116514	.	start_codon	3131	3131	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K116514	.	non_covalent_binding_site	3047	3084	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K116514	.	promoter	3119	3124	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K116514	.	CDS	2959	3046	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K116514	.	non_covalent_binding_site	3124	3158	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K116514	.	promoter	3095	3100	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K116514	.	CDS	1410	2267	.	+	0	ID=BBa_J36801;Name=BBa_J36801
BBa_K116514	.	promoter	2413	2612	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K116514	.	engineered_region	2413	2612	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K116514	.	start_codon	2585	2585	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K116514	.	non_covalent_binding_site	2501	2538	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K116514	.	promoter	2573	2578	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K116514	.	CDS	2413	2500	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K116514	.	non_covalent_binding_site	2578	2612	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K116514	.	promoter	2549	2554	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K116514	.	CDS	3185	4747	.	+	0	ID=BBa_J36804;Name=BBa_J36804
BBa_K116514	.	terminator	2364	2404	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K116514	.	engineered_region	2364	2404	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116514	.	stop_codon	2397	2397	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116514	.	polyA_site	2391	2404	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116514	.	stem_loop	2371	2390	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116514	.	ribosome_entry_site	3167	3178	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K116514	.	conserved_region	3171	3174	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K116514	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K116514	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K116514	.	CDS	19	1182	.	+	0	ID=BBa_K116502;Name=BBa_K116502
BBa_K116514	.	ribosome_entry_site	1390	1401	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K116514	.	conserved_region	1394	1397	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K116514	.	CDS	2639	2950	.	+	0	ID=BBa_J36802;Name=BBa_J36802
BBa_K116514	.	ribosome_entry_site	2621	2632	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K116514	.	conserved_region	2625	2628	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K116514	.	promoter	1328	1381	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K116514	.	promoter	1370	1375	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K116514	.	non_covalent_binding_site	1328	1346	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K116514	.	non_covalent_binding_site	1353	1371	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K116514	.	engineered_region	1328	1381	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K116514	.	promoter	1347	1352	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K116514	.	terminator	1191	1270	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K116514	.	engineered_region	1191	1270	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116514	.	stem_loop	1202	1245	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116514	.	terminator	2276	2355	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K116514	.	engineered_region	2276	2355	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116514	.	stem_loop	2287	2330	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116514	.	terminator	1279	1319	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K116514	.	engineered_region	1279	1319	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116514	.	stop_codon	1312	1312	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116514	.	polyA_site	1306	1319	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116514	.	stem_loop	1286	1305	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_K116514
aaagaggagaaatactagatgggagagtctctgtcaccacaagcactggctcaaccgttgctactgcaactgttcgtcg
atacccggcccctgtcacagcacattgtgcagcgggttaaaaatattttggcagcagtagaggcaaccgtccccatcag
cttgcaggtgatcaatgtggcggaccagccacaactggtggagtactaccgcctagtcgtcacgcctgccctggttaaa
attggtccaggctctcgccaagttctgagtggcatcgacctcaccgatcaattagccaaccagttgccccagtggctgg
ttcagcaagaggccttttttgccgatcgagagccacctgaagtcaacattccgttcacggagctaggccaacccgagac
ccccgcgttgcagcaggctgatgccttttttcagcttcagcaacaatacgctgatctctcggagcggacaaaattcctt
gagcaggtcattgctctcgtcgcccacgacctccgcaatccgctaaccgctgctcttttggcggtcgacaccattcaaa
tccgcagtcaatccttttctgtggcgacagccaaggaaatgcagggactgtgcagtctgttcgatcaggcacgatcgca
attgcgtgaaatcgagcgcatgattgccgagattttggaggcaactcgccactctggcgaaagcttgcggatcaatccc
cgcgaagtcgtctttgagccgctcttacaacaggttctggaacagttgcatgaacgctggcggagtaagcagcagcagt
tgatcacggatgttccaggcgacctgcccacgctctacgccgaccctgaccgtctgcggcaggttctggtcaatctgct
agacaacgccatcaaatacactccgcccgggggcacaatcacgatcgcagcgctccatcgcacgagccagaaagtgcag
atcagcatcagcgacacgggctctggcatccctcgcgaccagctcagcgtcattttcaagaacttggtgcggctttccc
gcgatagcagccaagagggctacggcattggactatcggtttgccagcgaattgttcaggcccactttggccgcatctg
ggttgcttcggaactgggccaaggcagcaccttccacttcacgatgccggtttatcgctacaccatgccctgctaatac
tagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac
gctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatag
agattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagaggtgctctcgcaaa
ttgcaatctgcatttgggtggaatcgacggcaattttgcaggattgccagcgggcgctgtcggccgatcgctatcaact
ccaagtctgtgagtctggcgaaatgctcttggagtatgcccaaacccatcgtgaccaaatcgactgcctgattttagtg
gcagccaatcccagcttcagggcagttgttcagcagctctgctttgagggagtggtggtaccagcgattgtcgtaggcg
atcgcgacagtgaggatcccgatgaaccagccaaagaacagctctatcacagcgctgaactgcacctcggtatccatca
gctcgagcaattgccctaccaagttgatgctgcactggctgaatttctgcgcttagccccggtcgagaccatggccgac
cacatcatgctgatgggggccaaccacgatcccgagctatcgagccagcagcgggacctcgctcagcgactacaagagc
gcctaggctatctcggggtctactacaagcgtgatcccgatcgctttctgcgcaacctacccgcctacgaaagccaaaa
gctgcaccaagcgatgcagactagctatcgtgaaatcgttttgagctatttttcgccgaatagcaacctcaaccagagc
attgacaacttcgtcaacatggctttctttgccgatgttccagtcaccaaagtggtagaaattcacatggagctgatgg
acgagtttgccaagaagctccgcgtagagggacgttcagaggacattttgctggattatcggctgactttaattgatgt
aattgcacatctttgtgagatgtatcgacggtctatcccacgagaaacctgatgatactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgat
tcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctc
actcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttc
acacatactagagaaagaggagaaatactagatgagccctcgtaaaacctacattctcaagctctacgtcgccggcaat
actccaaactcagtccgtgccctcaaaacgctcaagaacattctcgaagttgaatttcaaggtgtttatgctctaaagg
tgatcgatgttctcaaaaatcctcagttggcagaagaggataaaatcctagcgacgccaaccctcgccaaggttctacc
actgcctgtccgacggattattggtgatttatccgaccgtgagaaagttttgattggccttgatttactctacggcgaa
cttcaagattccgacgacttctaataatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaa
tgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcatt
aggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacata
ctagagaaagaggagaaatactagatgacttccgctgagatgactagccctaataataattctgagcaccaagccatcg
ctaagatgcgcacgatgattgaaggctttgatgatattagtcatggcggtcttccaatcgggcgatcgaccctcgttag
tggtacttcaggaaccggcaagacccttttttctattcaatttctctataacggtattatcgagtttgatgagcctggg
gttttcgttactttcgaagaaaccccgcaagatatcattaaaaacgcccgtagttttggctgggatttagccaagctgg
tcgatgagggcaaactatttattcttgatgcttcacccgatccagaaggtcaagaggttgttggcggcttcgatctctc
tgctctgattgagcggattaattatgcaattcaaaagtatcgagcgcggcgggtttcaattgactcggtcacgtccgtt
ttccagcaatatgatgcctcttctgtggttcgccgcgaactctttcggttggtagctcgcctaaaacaaattggggcaa
ctacggtcatgaccaccgagcgtatcgaggaatatggcccgatcgctcgttacggtgttgaggaatttgtctccgataa
cgtcgtgattctccgcaacgttttggaaggggagcgccgtcgccgcaccctcgaaatcctcaagctacgtggcaccagc
cacatgaaaggggaatatccgttcacgattacggatcatggcatcaatatcttcccgctcggggcaatgcgccttacgc
agcgatcgtcgaacgtgcgtgtttcatctggtgtcgtccgactcgatgaaatgtgtggtgggggcttctttaaggactc
aatcattctggcaactggcgctacaggcactggtaaaactctgttagttagccgtttcgttgagaatgcttgtgctaac
aaagagcgggcgattctgttcgcttatgaagagtcacgagctcagctgctccgcaatgcctattcatggggaatggact
ttgaggagatggagcgccaaaacctcctcaaaattgtttgcgcctatcctgaatccgcaggtcttgaagaccatttgca
gattattaagtcggagatcaatgactttaagccagctcgtattgcaatcgactccctctctgctttggcgcggggcgtt
agcaacaatgccttccgccaatttgtaattggtgtcactggctacgcgaaacaagaagaaatcacgggactattcacaa
ataccagtgatcaatttatgggagcgcattcgattactgactcccatatctcaacaattacggatacgattatcttgct
ccaatacgtcgagattcgtggcgaaatgtcccgcgccattaacgtcttcaagatgcgcggatcttggcatgacaaagca
atccgcgagttcatgatcagcgacaaagggccggacatcaaggattctttccggaactttgagcggattatttcaggtt
cgccaacacggattaccgtcgatgagaaaagcgaactctcgcgaattgtgcgcggcgttcaagaaaaagggccggagag
ctagtag