LOCUS       BBa_K116520             1848 bp    DNA     linear       28-Jun-2024
DEFINITION  GFP under control of RpaA activated pOmpF promoter
ACCESSION   BBa_K116520
VERSION     BBa_K116520.1  
FEATURES             Location/Qualifiers
     misc_feature    27..88
                     /label=OmpR binding
     promoter        1..126
                     /label=BBa_K116500
     RBS             135..147
                     /label=RBS-3\Weak
     RBS             141..144
                     /label=RBS
     misc_feature    135..147
                     /label=BBa_B0032
     RBS             135..147
                     /label=BBa_B0032
     CDS             154..873
                     /label=GFP protein
     CDS             154..873
                     /label=BBa_E0040
     stem_loop       893..936
                     /label=stem_loop
     misc_feature    882..961
                     /label=BBa_B0010
     terminator      882..961
                     /label=BBa_B0010
     stem_loop       977..996
                     /label=T7 TE
     misc_feature    1003..1003
                     /label=stop
     polyA_site      997..1010
                     /label=polya
     misc_feature    970..1010
                     /label=BBa_B0012
     terminator      970..1010
                     /label=BBa_B0012
     misc_feature    1019..1037
                     /label=TetR 1
     misc_feature    1019..1072
                     /label=BBa_R0040
     promoter        1038..1043
                     /label=-35
     misc_feature    1044..1062
                     /label=TetR 2
     promoter        1061..1066
                     /label=-10
     promoter        1019..1072
                     /label=BBa_R0040
     misc_feature    1085..1088
                     /label=conserved
     RBS             1081..1092
                     /label=BBa_B0034
     CDS             1099..1848
                     /label=BBa_K116501
ORIGIN
        1 gacggtgttc acaaagttcc ttaaatttta cttttggtta catatttttt ctttttgaaa
       61 ccaaatcttt atctttgtag cactttcacg gtagcgaaac gttagtttga atggaaagat
      121 gcctgctact agagtcacac aggaaagtac tagatgcgta aaggagaaga acttttcact
      181 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
      241 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
      301 actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa
      361 tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc
      421 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt
      481 gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat
      541 tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat
      601 gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac
      661 aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc
      721 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa
      781 gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt
      841 acacatggca tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg
      901 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
      961 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtc
     1021 cctatcagtg atagagattg acatccctat cagtgataga gatactgagc actactagag
     1081 aaagaggaga aatactagat gaaaccccgc atcctcgtga tcgatgatga ctcagccatc
     1141 ttggagctgg tcgccgtcaa tctggagatg tctggctatg acgtacgcaa agctgaggac
     1201 ggcattaaag gtcaggcttt agctgttcag ctagttcccg acctgatcat gctggatcta
     1261 atgctgccgc gggttgatgg ctttaccgtc tgtcagcgac tgcggcgcga tgagcgtact
     1321 gccgaaattc cggtgctgat gctgaccgcc ctcggacaga ctcaggataa ggttgaaggc
     1381 ttcaacgcgg gtgctgacga ttatctgact aagcccttcg aagttgaaga gatgctggcc
     1441 cgcgtgcgtg ccttgctgcg gcgcaccgat cgcattcccc atgcagcccg ccatagcgaa
     1501 attctcagct acggtccgct gaccctgatt cccgagcggt ttgaggccat ttggttcaac
     1561 cgcacggtca agctgactca cttggaattt gagttgttgc actgcctgtt gcaacgccac
     1621 ggccaaacgg ttgcgccgag cgaaatcctc aaagaagtct ggggctatga tcccgacgat
     1681 gacatcgaga cgattcgcgt ccacatccgt catctgcgca ccaagctcga gcccgatccc
     1741 cggcacccgc gctacatcaa aacggtctat ggagcgggct actgccttga gctgccggcc
     1801 gagacggaac tccaccaaca cgccgatcag tttccttcgg cgtcctga
//