BBa_K116501
1
BBa_K116501
RpaA(regulator of phycobilisome-associated)
2008-10-28T12:00:00Z
2015-05-08T01:09:33Z
Synechococcus elongatus PCC7942
The SasA???RpaA signal transduction system represents an activation output pathway from the cyanobacteria Kai oscillator. SasA is transfer its phosphoryl group to RpaA, which is predicted to activate this RR.
false
false
_210_
0
2083
9
Not in stock
false
The binding affinity to DNA still have to be confirmed
false
Chun-Ju Yang
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986785
1
-35
range1986785
1
20
25
annotation1986787
1
-10
range1986787
1
43
48
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986786
1
TetR 2
range1986786
1
26
44
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
annotation7027
1
BBa_B0032
range7027
1
1
13
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_K116500
1
BBa_K116500
OmpF promoter that is activated or repressesed by OmpR according to osmolarity.
2008-10-28T12:00:00Z
2015-05-08T01:09:33Z
Promoter OmpF is activated by phosphorylated OmpR at low osmolarity, and is repressesed by phosphorylated OmpR at high osmolarity.In Escherichia coli, osmoregulation is mediated in part by the actions of such a two-component system consisting of EnvZ and OmpR. These proteins
act to control the relative levels of the outer membrane porin genes, ompF and ompC. At low osmo larity, OmpF predominates in the outer membrane, while at high osmolarity the OmpF porin is replaced by OmpC.OmpF has a larger pore and a faster ??ow rate than OmpC.
Promoter OmpF is activated by phosphorylated OmpR at low osmolarity, and is repressesed by phosphorylated OmpR at high osmolarity.In Escherichia coli, osmoregulation is mediated in part by the actions of such a two-component system consisting of EnvZ and OmpR. These proteins act to control the relative levels of the outer membrane porin genes, ompF and ompC. At low osmo larity, OmpF predominates in the outer membrane, while at high osmolarity the OmpF porin is replaced by OmpC.OmpF has a larger pore and a faster ??ow rate than OmpC.
Reference: K. Mattison, R. Oropeza, N. Byers, L. J. Kenney, J Mol Biol 315, 497 (Jan 25, 2002).
false
false
_210_
0
2083
9
It's complicated
false
The osmolarity range of OmpF sensing still have to be confirmed
false
Chun-Ju Yang
annotation1994040
1
OmpR binding
range1994040
1
27
88
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K116520
1
BBa_K116520
GFP under control of RpaA activated pOmpF promoter
2008-10-28T12:00:00Z
2015-05-08T01:09:33Z
BBa_K116510 BBa_K116511
GFP under control of RpaA activated pOmpF promoter
false
false
_210_
0
2083
9
It's complicated
false
N. Takai et al., Proc Natl Acad Sci U S A 103, 12109 (Aug 8, 2006).
false
Chun-Ju Yang
component1995093
1
BBa_R0040
component1995083
1
BBa_B0032
component1995086
1
BBa_E0040
component1995081
1
BBa_K116500
component1995099
1
BBa_B0034
component1995089
1
BBa_B0012
component1995100
1
BBa_K116501
component1995087
1
BBa_B0010
annotation1995087
1
BBa_B0010
range1995087
1
882
961
annotation1995100
1
BBa_K116501
range1995100
1
1099
1848
annotation1995089
1
BBa_B0012
range1995089
1
970
1010
annotation1995086
1
BBa_E0040
range1995086
1
154
873
annotation1995093
1
BBa_R0040
range1995093
1
1019
1072
annotation1995083
1
BBa_B0032
range1995083
1
135
147
annotation1995081
1
BBa_K116500
range1995081
1
1
126
annotation1995099
1
BBa_B0034
range1995099
1
1081
1092
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K116501_sequence
1
atgaaaccccgcatcctcgtgatcgatgatgactcagccatcttggagctggtcgccgtcaatctggagatgtctggctatgacgtacgcaaagctgaggacggcattaaaggtcaggctttagctgttcagctagttcccgacctgatcatgctggatctaatgctgccgcgggttgatggctttaccgtctgtcagcgactgcggcgcgatgagcgtactgccgaaattccggtgctgatgctgaccgccctcggacagactcaggataaggttgaaggcttcaacgcgggtgctgacgattatctgactaagcccttcgaagttgaagagatgctggcccgcgtgcgtgccttgctgcggcgcaccgatcgcattccccatgcagcccgccatagcgaaattctcagctacggtccgctgaccctgattcccgagcggtttgaggccatttggttcaaccgcacggtcaagctgactcacttggaatttgagttgttgcactgcctgttgcaacgccacggccaaacggttgcgccgagcgaaatcctcaaagaagtctggggctatgatcccgacgatgacatcgagacgattcgcgtccacatccgtcatctgcgcaccaagctcgagcccgatccccggcacccgcgctacatcaaaacggtctatggagcgggctactgccttgagctgccggccgagacggaactccaccaacacgccgatcagtttccttcggcgtcctga
BBa_B0034_sequence
1
aaagaggagaaa
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_B0032_sequence
1
tcacacaggaaag
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_K116520_sequence
1
gacggtgttcacaaagttccttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaaacgttagtttgaatggaaagatgcctgctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaccccgcatcctcgtgatcgatgatgactcagccatcttggagctggtcgccgtcaatctggagatgtctggctatgacgtacgcaaagctgaggacggcattaaaggtcaggctttagctgttcagctagttcccgacctgatcatgctggatctaatgctgccgcgggttgatggctttaccgtctgtcagcgactgcggcgcgatgagcgtactgccgaaattccggtgctgatgctgaccgccctcggacagactcaggataaggttgaaggcttcaacgcgggtgctgacgattatctgactaagcccttcgaagttgaagagatgctggcccgcgtgcgtgccttgctgcggcgcaccgatcgcattccccatgcagcccgccatagcgaaattctcagctacggtccgctgaccctgattcccgagcggtttgaggccatttggttcaaccgcacggtcaagctgactcacttggaatttgagttgttgcactgcctgttgcaacgccacggccaaacggttgcgccgagcgaaatcctcaaagaagtctggggctatgatcccgacgatgacatcgagacgattcgcgtccacatccgtcatctgcgcaccaagctcgagcccgatccccggcacccgcgctacatcaaaacggtctatggagcgggctactgccttgagctgccggccgagacggaactccaccaacacgccgatcagtttccttcggcgtcctga
BBa_K116500_sequence
1
gacggtgttcacaaagttccttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaaacgttagtttgaatggaaagatgcctgc
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z