##gff-version 3
##sequence-region BBa_K116520 1 1848
BBa_K116520	.	terminator	882	961	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K116520	.	stem_loop	893	936	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116520	.	engineered_region	882	961	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116520	.	CDS	1099	1848	.	+	0	ID=BBa_K116501;Name=BBa_K116501
BBa_K116520	.	ribosome_entry_site	135	147	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K116520	.	engineered_region	135	147	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K116520	.	ribosome_entry_site	135	147	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K116520	.	ribosome_entry_site	141	144	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K116520	.	CDS	154	873	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K116520	.	CDS	154	873	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K116520	.	ribosome_entry_site	1081	1092	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K116520	.	conserved_region	1085	1088	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K116520	.	promoter	1019	1072	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K116520	.	engineered_region	1019	1072	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K116520	.	non_covalent_binding_site	1019	1037	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K116520	.	non_covalent_binding_site	1044	1062	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K116520	.	promoter	1061	1066	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K116520	.	promoter	1038	1043	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K116520	.	terminator	970	1010	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K116520	.	polyA_site	997	1010	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116520	.	engineered_region	970	1010	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116520	.	stem_loop	977	996	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116520	.	stop_codon	1003	1003	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116520	.	promoter	1	126	.	+	0	ID=BBa_K116500;Name=BBa_K116500
BBa_K116520	.	non_covalent_binding_site	27	88	.	+	0	ID=annotation1994040;Name=OmpR binding;Parent=BBa_K116500
>BBa_K116520
gacggtgttcacaaagttccttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgta
gcactttcacggtagcgaaacgttagtttgaatggaaagatgcctgctactagagtcacacaggaaagtactagatgcg
taaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttct
gtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctg
ttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagca
tgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaag
acacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatg
gaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatgg
aatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatact
ccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacg
aaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaata
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatca
gtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaacc
ccgcatcctcgtgatcgatgatgactcagccatcttggagctggtcgccgtcaatctggagatgtctggctatgacgta
cgcaaagctgaggacggcattaaaggtcaggctttagctgttcagctagttcccgacctgatcatgctggatctaatgc
tgccgcgggttgatggctttaccgtctgtcagcgactgcggcgcgatgagcgtactgccgaaattccggtgctgatgct
gaccgccctcggacagactcaggataaggttgaaggcttcaacgcgggtgctgacgattatctgactaagcccttcgaa
gttgaagagatgctggcccgcgtgcgtgccttgctgcggcgcaccgatcgcattccccatgcagcccgccatagcgaaa
ttctcagctacggtccgctgaccctgattcccgagcggtttgaggccatttggttcaaccgcacggtcaagctgactca
cttggaatttgagttgttgcactgcctgttgcaacgccacggccaaacggttgcgccgagcgaaatcctcaaagaagtc
tggggctatgatcccgacgatgacatcgagacgattcgcgtccacatccgtcatctgcgcaccaagctcgagcccgatc
cccggcacccgcgctacatcaaaacggtctatggagcgggctactgccttgagctgccggccgagacggaactccacca
acacgccgatcagtttccttcggcgtcctga