BBa_K116520
type: ComponentDefinition
persistent identity: BBa K116520
display id: BBa_K116520
version: 1
was generated by: iGEM to SBOL conversion
description: GFP under control of RpaA activated pOmpF promoter
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:33Z
mutable provenance: BBa_K116510 BBa_K116511
owned by: james
owned by: myers
mutable description: GFP under control of RpaA activated pOmpF promoter
discontinued: false
dominant: false
group u list: _210_
m user id: 0
owner id: 2083
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N. Takai et al., Proc Natl Acad Sci U S A 103, 12109 (Aug 8, 2006).
star: false
creator: Chun-Ju Yang
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K116501
component: BBa_B0034
component: BBa_R0040
component: BBa_B0012
component: BBa_B0010
component: BBa_E0040
component: BBa_B0032
component: BBa_K116500
sequence annotation: BBa_K116500
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_K116501
sequence: https://synbiohub.org/public/igem/BBa_K116520_sequence/1

BBa_K116501
type: Component
persistent identity: component1995100
display id: component1995100
version: 1
top level: BBa_K116520
definition: BBa_K116501
access: public

BBa_B0034
type: Component
persistent identity: component1995099
display id: component1995099
version: 1
top level: BBa_K116520
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component1995093
display id: component1995093
version: 1
top level: BBa_K116520
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component1995089
display id: component1995089
version: 1
top level: BBa_K116520
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1995087
display id: component1995087
version: 1
top level: BBa_K116520
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1995086
display id: component1995086
version: 1
top level: BBa_K116520
definition: GFP
access: public

BBa_B0032
type: Component
persistent identity: component1995083
display id: component1995083
version: 1
top level: BBa_K116520
definition: BBa_B0032
access: public

BBa_K116500
type: Component
persistent identity: component1995081
display id: component1995081
version: 1
top level: BBa_K116520
definition: BBa_K116500
access: public

BBa_K116500
type: SequenceAnnotation
persistent identity: annotation1995081
display id: annotation1995081
version: 1
top level: BBa_K116520
location: https://synbiohub.org/public/igem/BBa_K116520/annotation1995081/range1995081/1
component: BBa_K116500

https://synbiohub.org/public/igem/BBa_K116520/annotation1995081/range1995081/1
type: Range
persistent identity: range1995081
display id: range1995081
version: 1
top level: BBa_K116520
direction: direction/reverse
start: 1
end: 126
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1995083
display id: annotation1995083
version: 1
top level: BBa_K116520
location: https://synbiohub.org/public/igem/BBa_K116520/annotation1995083/range1995083/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K116520/annotation1995083/range1995083/1
type: Range
persistent identity: range1995083
display id: range1995083
version: 1
top level: BBa_K116520
direction: direction/forward
start: 135
end: 147
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1995086
display id: annotation1995086
version: 1
top level: BBa_K116520
location: https://synbiohub.org/public/igem/BBa_K116520/annotation1995086/range1995086/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K116520/annotation1995086/range1995086/1
type: Range
persistent identity: range1995086
display id: range1995086
version: 1
top level: BBa_K116520
direction: direction/reverse
start: 154
end: 873
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1995087
display id: annotation1995087
version: 1
top level: BBa_K116520
location: https://synbiohub.org/public/igem/BBa_K116520/annotation1995087/range1995087/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K116520/annotation1995087/range1995087/1
type: Range
persistent identity: range1995087
display id: range1995087
version: 1
top level: BBa_K116520
direction: direction/forward
start: 882
end: 961
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1995089
display id: annotation1995089
version: 1
top level: BBa_K116520
location: https://synbiohub.org/public/igem/BBa_K116520/annotation1995089/range1995089/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K116520/annotation1995089/range1995089/1
type: Range
persistent identity: range1995089
display id: range1995089
version: 1
top level: BBa_K116520
direction: direction/forward
start: 970
end: 1010
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1995093
display id: annotation1995093
version: 1
top level: BBa_K116520
location: https://synbiohub.org/public/igem/BBa_K116520/annotation1995093/range1995093/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K116520/annotation1995093/range1995093/1
type: Range
persistent identity: range1995093
display id: range1995093
version: 1
top level: BBa_K116520
direction: direction/forward
start: 1019
end: 1072
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1995099
display id: annotation1995099
version: 1
top level: BBa_K116520
location: https://synbiohub.org/public/igem/BBa_K116520/annotation1995099/range1995099/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K116520/annotation1995099/range1995099/1
type: Range
persistent identity: range1995099
display id: range1995099
version: 1
top level: BBa_K116520
direction: direction/reverse
start: 1081
end: 1092
orientation: inline

BBa_K116501
type: SequenceAnnotation
persistent identity: annotation1995100
display id: annotation1995100
version: 1
top level: BBa_K116520
location: https://synbiohub.org/public/igem/BBa_K116520/annotation1995100/range1995100/1
component: BBa_K116501

https://synbiohub.org/public/igem/BBa_K116520/annotation1995100/range1995100/1
type: Range
persistent identity: range1995100
display id: range1995100
version: 1
top level: BBa_K116520
direction: direction/reverse
start: 1099
end: 1848
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_K116500
type: ComponentDefinition
persistent identity: BBa K116500
display id: BBa_K116500
version: 1
was generated by: iGEM to SBOL conversion
description: OmpF promoter that is activated or repressesed by OmpR according to osmolarity.
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:33Z
mutable provenance: Promoter OmpF is activated by phosphorylated OmpR at low osmolarity, and is repressesed by phosphorylated OmpR at high osmolarity.In Escherichia coli, osmoregulation is mediated in part by the actions of such a two-component system consisting of EnvZ and OmpR. These proteins act to control the relative levels of the outer membrane porin genes, ompF and ompC. At low osmo larity, OmpF predominates in the outer membrane, while at high osmolarity the OmpF porin is replaced by OmpC.OmpF has a larger pore and a faster ??ow rate than OmpC.
owned by: james
owned by: myers
mutable description: Promoter OmpF is activated by phosphorylated OmpR at low osmolarity, and is repressesed by phosphorylated OmpR at high osmolarity.In Escherichia coli, osmoregulation is mediated in part by the actions of such a two-component system consisting of EnvZ and OmpR. These proteins act to control the relative levels of the outer membrane porin genes, ompF and ompC. At low osmo larity, OmpF predominates in the outer membrane, while at high osmolarity the OmpF porin is replaced by OmpC.OmpF has a larger pore and a faster ??ow rate than OmpC. Reference: K. Mattison, R. Oropeza, N. Byers, L. J. Kenney, J Mol Biol 315, 497 (Jan 25, 2002).
discontinued: false
dominant: false
group u list: _210_
m user id: 0
owner id: 2083
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The osmolarity range of OmpF sensing still have to be confirmed
star: false
creator: Chun-Ju Yang
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: OmpR binding
sequence: https://synbiohub.org/public/igem/BBa_K116500_sequence/1

OmpR binding
type: SequenceAnnotation
persistent identity: annotation1994040
display id: annotation1994040
version: 1
top level: BBa_K116500
location: https://synbiohub.org/public/igem/BBa_K116500/annotation1994040/range1994040/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K116500/annotation1994040/range1994040/1
type: Range
persistent identity: range1994040
display id: range1994040
version: 1
top level: BBa_K116500
direction: direction/forward
start: 27
end: 88
orientation: inline

BBa_K116501
type: ComponentDefinition
persistent identity: BBa K116501
display id: BBa_K116501
version: 1
was generated by: iGEM to SBOL conversion
description: RpaA(regulator of phycobilisome-associated)
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:33Z
mutable provenance: Synechococcus elongatus PCC7942
owned by: james
owned by: myers
mutable description: The SasA???RpaA signal transduction system represents an activation output pathway from the cyanobacteria Kai oscillator. SasA is transfer its phosphoryl group to RpaA, which is predicted to activate this RR.
discontinued: false
dominant: false
group u list: _210_
m user id: 0
owner id: 2083
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The binding affinity to DNA still have to be confirmed
star: false
creator: Chun-Ju Yang
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K116501_sequence/1

https://synbiohub.org/public/igem/BBa_K116520_sequence/1
type: Sequence
persistent identity: BBa K116520 sequence
display id: BBa_K116520_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacggtgttcacaaagttccttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaaacgttagtttgaatggaaagatgcctgctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaccccgcatcctcgtgatcgatgatgactcagccatcttggagctggtcgccgtcaatctggagatgtctggctatgacgtacgcaaagctgaggacggcattaaaggtcaggctttagctgttcagctagttcccgacctgatcatgctggatctaatgctgccgcgggttgatggctttaccgtctgtcagcgactgcggcgcgatgagcgtactgccgaaattccggtgctgatgctgaccgccctcggacagactcaggataaggttgaaggcttcaacgcgggtgctgacgattatctgactaagcccttcgaagttgaagagatgctggcccgcgtgcgtgccttgctgcggcgcaccgatcgcattccccatgcagcccgccatagcgaaattctcagctacggtccgctgaccctgattcccgagcggtttgaggccatttggttcaaccgcacggtcaagctgactcacttggaatttgagttgttgcactgcctgttgcaacgccacggccaaacggttgcgccgagcgaaatcctcaaagaagtctggggctatgatcccgacgatgacatcgagacgattcgcgtccacatccgtcatctgcgcaccaagctcgagcccgatccccggcacccgcgctacatcaaaacggtctatggagcgggctactgccttgagctgccggccgagacggaactccaccaacacgccgatcagtttccttcggcgtcctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K116500_sequence/1
type: Sequence
persistent identity: BBa K116500 sequence
display id: BBa_K116500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacggtgttcacaaagttccttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaaacgttagtttgaatggaaagatgcctgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K116501_sequence/1
type: Sequence
persistent identity: BBa K116501 sequence
display id: BBa_K116501_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccccgcatcctcgtgatcgatgatgactcagccatcttggagctggtcgccgtcaatctggagatgtctggctatgacgtacgcaaagctgaggacggcattaaaggtcaggctttagctgttcagctagttcccgacctgatcatgctggatctaatgctgccgcgggttgatggctttaccgtctgtcagcgactgcggcgcgatgagcgtactgccgaaattccggtgctgatgctgaccgccctcggacagactcaggataaggttgaaggcttcaacgcgggtgctgacgattatctgactaagcccttcgaagttgaagagatgctggcccgcgtgcgtgccttgctgcggcgcaccgatcgcattccccatgcagcccgccatagcgaaattctcagctacggtccgctgaccctgattcccgagcggtttgaggccatttggttcaaccgcacggtcaagctgactcacttggaatttgagttgttgcactgcctgttgcaacgccacggccaaacggttgcgccgagcgaaatcctcaaagaagtctggggctatgatcccgacgatgacatcgagacgattcgcgtccacatccgtcatctgcgcaccaagctcgagcccgatccccggcacccgcgctacatcaaaacggtctatggagcgggctactgccttgagctgccggccgagacggaactccaccaacacgccgatcagtttccttcggcgtcctga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z