LOCUS       BBa_K116523             1830 bp    DNA     linear       02-May-2025
DEFINITION  GFP under control of RpaA activated pOmpC promoter
ACCESSION   BBa_K116523
VERSION     BBa_K116523.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    34..51
                     /label=C2 OmpR
     misc_feature    54..71
                     /label=C3 OmpR
     promoter        75..80
                     /label=-35
     promoter        98..103
                     /label=-10
     promoter        1..108
                     /label=BBa_R0082
     RBS             117..129
                     /label=RBS-3\Weak
     RBS             123..126
                     /label=RBS
     misc_feature    117..129
                     /label=BBa_B0032
     RBS             117..129
                     /label=BBa_B0032
     CDS             136..855
                     /label=GFP protein
     CDS             136..855
                     /label=BBa_E0040
     stem_loop       875..918
                     /label=stem_loop
     misc_feature    864..943
                     /label=BBa_B0010
     terminator      864..943
                     /label=BBa_B0010
     stem_loop       959..978
                     /label=T7 TE
     misc_feature    985..985
                     /label=stop
     polyA_site      979..992
                     /label=polya
     misc_feature    952..992
                     /label=BBa_B0012
     terminator      952..992
                     /label=BBa_B0012
     misc_feature    1001..1019
                     /label=TetR 1
     misc_feature    1001..1054
                     /label=BBa_R0040
     promoter        1020..1025
                     /label=-35
     misc_feature    1026..1044
                     /label=TetR 2
     promoter        1043..1048
                     /label=-10
     promoter        1001..1054
                     /label=BBa_R0040
     misc_feature    1067..1070
                     /label=conserved
     RBS             1063..1074
                     /label=BBa_B0034
     CDS             1081..1830
                     /label=BBa_K116501
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta
       61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagtcac
      121 acaggaaagt actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt
      181 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
      241 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
      301 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca
      361 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      421 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
      481 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
      541 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
      601 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
      661 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
      721 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
      781 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
      841 ctatacaaat aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      901 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
      961 ctcaccttcg ggtgggcctt tctgcgttta tatactagag tccctatcag tgatagagat
     1021 tgacatccct atcagtgata gagatactga gcactactag agaaagagga gaaatactag
     1081 atgaaacccc gcatcctcgt gatcgatgat gactcagcca tcttggagct ggtcgccgtc
     1141 aatctggaga tgtctggcta tgacgtacgc aaagctgagg acggcattaa aggtcaggct
     1201 ttagctgttc agctagttcc cgacctgatc atgctggatc taatgctgcc gcgggttgat
     1261 ggctttaccg tctgtcagcg actgcggcgc gatgagcgta ctgccgaaat tccggtgctg
     1321 atgctgaccg ccctcggaca gactcaggat aaggttgaag gcttcaacgc gggtgctgac
     1381 gattatctga ctaagccctt cgaagttgaa gagatgctgg cccgcgtgcg tgccttgctg
     1441 cggcgcaccg atcgcattcc ccatgcagcc cgccatagcg aaattctcag ctacggtccg
     1501 ctgaccctga ttcccgagcg gtttgaggcc atttggttca accgcacggt caagctgact
     1561 cacttggaat ttgagttgtt gcactgcctg ttgcaacgcc acggccaaac ggttgcgccg
     1621 agcgaaatcc tcaaagaagt ctggggctat gatcccgacg atgacatcga gacgattcgc
     1681 gtccacatcc gtcatctgcg caccaagctc gagcccgatc cccggcaccc gcgctacatc
     1741 aaaacggtct atggagcggg ctactgcctt gagctgccgg ccgagacgga actccaccaa
     1801 cacgccgatc agtttccttc ggcgtcctga
//