BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_J36802 1 KaiB KaiB coding region 2006-10-30T12:00:00Z 2015-08-31T04:08:47Z synthetic; GeneArt AG KaiB coding region with an extra stop codon at the end. From Prochlorococcus (Synechococcus) elongatus PCC7942 genome. The actual DNA is synthetic in nature; was synthesized from GeneArt AG. Note that the coding region is bounded by the BioBricks Primers and is on a non-BioBrick vector pPCR-Script (ampR). The biobricks primers can be cut out along with the KaiB coding sequence with KpnI and SacI. The length of the entire plasmid is 3220bp. false false _65_ 0 1189 65 Not in stock false -none- false Zhipeng Sun, Hetmann Hsieh, Jeffrey Lau, David Ramos BBa_K116501 1 BBa_K116501 RpaA(regulator of phycobilisome-associated) 2008-10-28T12:00:00Z 2015-05-08T01:09:33Z Synechococcus elongatus PCC7942 The SasA???RpaA signal transduction system represents an activation output pathway from the cyanobacteria Kai oscillator. SasA is transfer its phosphoryl group to RpaA, which is predicted to activate this RR. false false _210_ 0 2083 9 Not in stock false The binding affinity to DNA still have to be confirmed false Chun-Ju Yang BBa_K116500 1 BBa_K116500 OmpF promoter that is activated or repressesed by OmpR according to osmolarity. 2008-10-28T12:00:00Z 2015-05-08T01:09:33Z Promoter OmpF is activated by phosphorylated OmpR at low osmolarity, and is repressesed by phosphorylated OmpR at high osmolarity.In Escherichia coli, osmoregulation is mediated in part by the actions of such a two-component system consisting of EnvZ and OmpR. These proteins act to control the relative levels of the outer membrane porin genes, ompF and ompC. At low osmo larity, OmpF predominates in the outer membrane, while at high osmolarity the OmpF porin is replaced by OmpC.OmpF has a larger pore and a faster ??ow rate than OmpC. Promoter OmpF is activated by phosphorylated OmpR at low osmolarity, and is repressesed by phosphorylated OmpR at high osmolarity.In Escherichia coli, osmoregulation is mediated in part by the actions of such a two-component system consisting of EnvZ and OmpR. These proteins act to control the relative levels of the outer membrane porin genes, ompF and ompC. At low osmo larity, OmpF predominates in the outer membrane, while at high osmolarity the OmpF porin is replaced by OmpC.OmpF has a larger pore and a faster ??ow rate than OmpC. Reference: K. Mattison, R. Oropeza, N. Byers, L. J. Kenney, J Mol Biol 315, 497 (Jan 25, 2002). false false _210_ 0 2083 9 It's complicated false The osmolarity range of OmpF sensing still have to be confirmed false Chun-Ju Yang annotation1994040 1 OmpR binding range1994040 1 27 88 BBa_J36801 1 KaiA KaiA coding region 2006-10-30T12:00:00Z 2015-08-31T04:08:47Z synthetic; GeneArt AG KaiA coding region with an extra stop codon at the end and two silent mutations at the EcoRI and PstI cut sites. From Prochlorococcus (Synechococcus) elongatus PCC7942 genome. The actual DNA is synthetic in nature; was synthesized from GeneArt AG. Note that the coding region is bounded by the BioBricks Primers and is on a non-BioBrick vector pGA4 (ampR). The biobricks primers can be cut out along with the KaiA coding sequence with HindIII and SacI. Length of entire plasmid is 3802bp. false false _65_ 0 1189 65 Not in stock false -none- false Zhipeng Sun BBa_K116530 1 BBa_K116530 cyanobacterial KaiABC oscillator with pOmpF as output 2008-10-28T12:00:00Z 2015-05-08T01:09:33Z BBa_K116520 BBa_B0015 BBa_R0040 BBa_K116514 cyanobacterial KaiABC oscillator with pOmpF as output false false _210_ 0 2083 9 It's complicated false N. Takai et al., Proc Natl Acad Sci U S A 103, 12109 (Aug 8, 2006). false Chun-Ju Yang component1995189 1 BBa_B0010 component1995174 1 BBa_K116501 component1995161 1 BBa_B0010 component1995201 1 BBa_B0034 component1995187 1 BBa_B0034 component1995155 1 BBa_K116500 component1995160 1 BBa_E0040 component1995227 1 BBa_B0034 component1995219 1 BBa_R0010 component1995188 1 BBa_K116502 component1995167 1 BBa_R0040 component1995203 1 BBa_B0010 component1995177 1 BBa_B0012 component1995202 1 BBa_J36801 component1995209 1 BBa_R0010 component1995195 1 BBa_R0040 component1995163 1 BBa_B0012 component1995205 1 BBa_B0012 component1995175 1 BBa_B0010 component1995228 1 BBa_J36804 component1995217 1 BBa_B0034 component1995181 1 BBa_R0040 component1995173 1 BBa_B0034 component1995191 1 BBa_B0012 component1995218 1 BBa_J36802 component1995157 1 BBa_B0032 annotation1995155 1 BBa_K116500 range1995155 1 1 126 annotation1995160 1 BBa_E0040 range1995160 1 154 873 annotation1995177 1 BBa_B0012 range1995177 1 1945 1985 annotation1995167 1 BBa_R0040 range1995167 1 1019 1072 annotation1995203 1 BBa_B0010 range1995203 1 4331 4410 annotation1995227 1 BBa_B0034 range1995227 1 5222 5233 annotation1995189 1 BBa_B0010 range1995189 1 3246 3325 annotation1995205 1 BBa_B0012 range1995205 1 4419 4459 annotation1995181 1 BBa_R0040 range1995181 1 1994 2047 annotation1995219 1 BBa_R0010 range1995219 1 5014 5213 annotation1995201 1 BBa_B0034 range1995201 1 3445 3456 annotation1995202 1 BBa_J36801 range1995202 1 3465 4322 annotation1995191 1 BBa_B0012 range1995191 1 3334 3374 annotation1995175 1 BBa_B0010 range1995175 1 1857 1936 annotation1995187 1 BBa_B0034 range1995187 1 2056 2067 annotation1995218 1 BBa_J36802 range1995218 1 4694 5005 annotation1995173 1 BBa_B0034 range1995173 1 1081 1092 annotation1995163 1 BBa_B0012 range1995163 1 970 1010 annotation1995209 1 BBa_R0010 range1995209 1 4468 4667 annotation1995217 1 BBa_B0034 range1995217 1 4676 4687 annotation1995174 1 BBa_K116501 range1995174 1 1099 1848 annotation1995188 1 BBa_K116502 range1995188 1 2074 3237 annotation1995161 1 BBa_B0010 range1995161 1 882 961 annotation1995228 1 BBa_J36804 range1995228 1 5240 6802 annotation1995195 1 BBa_R0040 range1995195 1 3383 3436 annotation1995157 1 BBa_B0032 range1995157 1 135 147 BBa_K116502 1 SasA SasA (Synechococcus adaptive sensor A) 2008-10-28T12:00:00Z 2015-05-08T01:09:33Z Synechococcus elongatus PCC7942 The SasA???RpaA signal transduction system represents an activation output pathway from the cyanobacteria Kai oscillator. SasA is transfer its phosphoryl group to RpaA, which is predicted to activate this RR. false false _210_ 0 2083 9 Not in stock false The pst I enzyme site at 718bp - 723bp was mutated (CTGCAG->TTACAA). This mutated construct is convenient for us to clone in standard biobrick part. false Chun-Ju Yang BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J36804 1 KaiC KaiC coding region 2006-10-30T12:00:00Z 2015-08-31T04:08:47Z synthetic; GeneArt AG KaiC coding region with an extra stop codon at the end. Two silent point mutations at EcoRI and PstI site. From Prochlorococcus (Synechococcus) elongatus PCC7942 genome. The actual DNA is synthetic in nature; was synthesized from GeneArt AG. Note that the coding region is bounded by the BioBricks Primers and is on a non-BioBrick vector pGA4(ampR). The biobricks primers can be cut out along with the KaiCcoding sequence with KpnI and XhoI. The length of the entire plasmid is 4558bp. false false _65_ 0 1189 65 Not in stock false -none- false Zhipeng Sun, Hetmann Hsieh, Jeffery Lau, Dave Ramos BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986787 1 -10 range1986787 1 43 48 annotation1986786 1 TetR 2 range1986786 1 26 44 annotation1986785 1 -35 range1986785 1 20 25 annotation1986783 1 TetR 1 range1986783 1 1 19 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961224 1 -35 range1961224 1 137 142 annotation1961227 1 start range1961227 1 173 173 annotation1961225 1 -10 range1961225 1 161 166 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 BBa_K116501_sequence 1 atgaaaccccgcatcctcgtgatcgatgatgactcagccatcttggagctggtcgccgtcaatctggagatgtctggctatgacgtacgcaaagctgaggacggcattaaaggtcaggctttagctgttcagctagttcccgacctgatcatgctggatctaatgctgccgcgggttgatggctttaccgtctgtcagcgactgcggcgcgatgagcgtactgccgaaattccggtgctgatgctgaccgccctcggacagactcaggataaggttgaaggcttcaacgcgggtgctgacgattatctgactaagcccttcgaagttgaagagatgctggcccgcgtgcgtgccttgctgcggcgcaccgatcgcattccccatgcagcccgccatagcgaaattctcagctacggtccgctgaccctgattcccgagcggtttgaggccatttggttcaaccgcacggtcaagctgactcacttggaatttgagttgttgcactgcctgttgcaacgccacggccaaacggttgcgccgagcgaaatcctcaaagaagtctggggctatgatcccgacgatgacatcgagacgattcgcgtccacatccgtcatctgcgcaccaagctcgagcccgatccccggcacccgcgctacatcaaaacggtctatggagcgggctactgccttgagctgccggccgagacggaactccaccaacacgccgatcagtttccttcggcgtcctga BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_B0034_sequence 1 aaagaggagaaa BBa_J36804_sequence 1 atgacttccgctgagatgactagccctaataataattctgagcaccaagccatcgctaagatgcgcacgatgattgaaggctttgatgatattagtcatggcggtcttccaatcgggcgatcgaccctcgttagtggtacttcaggaaccggcaagacccttttttctattcaatttctctataacggtattatcgagtttgatgagcctggggttttcgttactttcgaagaaaccccgcaagatatcattaaaaacgcccgtagttttggctgggatttagccaagctggtcgatgagggcaaactatttattcttgatgcttcacccgatccagaaggtcaagaggttgttggcggcttcgatctctctgctctgattgagcggattaattatgcaattcaaaagtatcgagcgcggcgggtttcaattgactcggtcacgtccgttttccagcaatatgatgcctcttctgtggttcgccgcgaactctttcggttggtagctcgcctaaaacaaattggggcaactacggtcatgaccaccgagcgtatcgaggaatatggcccgatcgctcgttacggtgttgaggaatttgtctccgataacgtcgtgattctccgcaacgttttggaaggggagcgccgtcgccgcaccctcgaaatcctcaagctacgtggcaccagccacatgaaaggggaatatccgttcacgattacggatcatggcatcaatatcttcccgctcggggcaatgcgccttacgcagcgatcgtcgaacgtgcgtgtttcatctggtgtcgtccgactcgatgaaatgtgtggtgggggcttctttaaggactcaatcattctggcaactggcgctacaggcactggtaaaactctgttagttagccgtttcgttgagaatgcttgtgctaacaaagagcgggcgattctgttcgcttatgaagagtcacgagctcagctgctccgcaatgcctattcatggggaatggactttgaggagatggagcgccaaaacctcctcaaaattgtttgcgcctatcctgaatccgcaggtcttgaagaccatttgcagattattaagtcggagatcaatgactttaagccagctcgtattgcaatcgactccctctctgctttggcgcggggcgttagcaacaatgccttccgccaatttgtaattggtgtcactggctacgcgaaacaagaagaaatcacgggactattcacaaataccagtgatcaatttatgggagcgcattcgattactgactcccatatctcaacaattacggatacgattatcttgctccaatacgtcgagattcgtggcgaaatgtcccgcgccattaacgtcttcaagatgcgcggatcttggcatgacaaagcaatccgcgagttcatgatcagcgacaaagggccggacatcaaggattctttccggaactttgagcggattatttcaggttcgccaacacggattaccgtcgatgagaaaagcgaactctcgcgaattgtgcgcggcgttcaagaaaaagggccggagagctagtag BBa_K116530_sequence 1 gacggtgttcacaaagttccttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaaacgttagtttgaatggaaagatgcctgctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaccccgcatcctcgtgatcgatgatgactcagccatcttggagctggtcgccgtcaatctggagatgtctggctatgacgtacgcaaagctgaggacggcattaaaggtcaggctttagctgttcagctagttcccgacctgatcatgctggatctaatgctgccgcgggttgatggctttaccgtctgtcagcgactgcggcgcgatgagcgtactgccgaaattccggtgctgatgctgaccgccctcggacagactcaggataaggttgaaggcttcaacgcgggtgctgacgattatctgactaagcccttcgaagttgaagagatgctggcccgcgtgcgtgccttgctgcggcgcaccgatcgcattccccatgcagcccgccatagcgaaattctcagctacggtccgctgaccctgattcccgagcggtttgaggccatttggttcaaccgcacggtcaagctgactcacttggaatttgagttgttgcactgcctgttgcaacgccacggccaaacggttgcgccgagcgaaatcctcaaagaagtctggggctatgatcccgacgatgacatcgagacgattcgcgtccacatccgtcatctgcgcaccaagctcgagcccgatccccggcacccgcgctacatcaaaacggtctatggagcgggctactgccttgagctgccggccgagacggaactccaccaacacgccgatcagtttccttcggcgtcctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgggagagtctctgtcaccacaagcactggctcaaccgttgctactgcaactgttcgtcgatacccggcccctgtcacagcacattgtgcagcgggttaaaaatattttggcagcagtagaggcaaccgtccccatcagcttgcaggtgatcaatgtggcggaccagccacaactggtggagtactaccgcctagtcgtcacgcctgccctggttaaaattggtccaggctctcgccaagttctgagtggcatcgacctcaccgatcaattagccaaccagttgccccagtggctggttcagcaagaggccttttttgccgatcgagagccacctgaagtcaacattccgttcacggagctaggccaacccgagacccccgcgttgcagcaggctgatgccttttttcagcttcagcaacaatacgctgatctctcggagcggacaaaattccttgagcaggtcattgctctcgtcgcccacgacctccgcaatccgctaaccgctgctcttttggcggtcgacaccattcaaatccgcagtcaatccttttctgtggcgacagccaaggaaatgcagggactgtgcagtctgttcgatcaggcacgatcgcaattgcgtgaaatcgagcgcatgattgccgagattttggaggcaactcgccactctggcgaaagcttgcggatcaatccccgcgaagtcgtctttgagccgctcttacaacaggttctggaacagttgcatgaacgctggcggagtaagcagcagcagttgatcacggatgttccaggcgacctgcccacgctctacgccgaccctgaccgtctgcggcaggttctggtcaatctgctagacaacgccatcaaatacactccgcccgggggcacaatcacgatcgcagcgctccatcgcacgagccagaaagtgcagatcagcatcagcgacacgggctctggcatccctcgcgaccagctcagcgtcattttcaagaacttggtgcggctttcccgcgatagcagccaagagggctacggcattggactatcggtttgccagcgaattgttcaggcccactttggccgcatctgggttgcttcggaactgggccaaggcagcaccttccacttcacgatgccggtttatcgctacaccatgccctgctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagaggtgctctcgcaaattgcaatctgcatttgggtggaatcgacggcaattttgcaggattgccagcgggcgctgtcggccgatcgctatcaactccaagtctgtgagtctggcgaaatgctcttggagtatgcccaaacccatcgtgaccaaatcgactgcctgattttagtggcagccaatcccagcttcagggcagttgttcagcagctctgctttgagggagtggtggtaccagcgattgtcgtaggcgatcgcgacagtgaggatcccgatgaaccagccaaagaacagctctatcacagcgctgaactgcacctcggtatccatcagctcgagcaattgccctaccaagttgatgctgcactggctgaatttctgcgcttagccccggtcgagaccatggccgaccacatcatgctgatgggggccaaccacgatcccgagctatcgagccagcagcgggacctcgctcagcgactacaagagcgcctaggctatctcggggtctactacaagcgtgatcccgatcgctttctgcgcaacctacccgcctacgaaagccaaaagctgcaccaagcgatgcagactagctatcgtgaaatcgttttgagctatttttcgccgaatagcaacctcaaccagagcattgacaacttcgtcaacatggctttctttgccgatgttccagtcaccaaagtggtagaaattcacatggagctgatggacgagtttgccaagaagctccgcgtagagggacgttcagaggacattttgctggattatcggctgactttaattgatgtaattgcacatctttgtgagatgtatcgacggtctatcccacgagaaacctgatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgagccctcgtaaaacctacattctcaagctctacgtcgccggcaatactccaaactcagtccgtgccctcaaaacgctcaagaacattctcgaagttgaatttcaaggtgtttatgctctaaaggtgatcgatgttctcaaaaatcctcagttggcagaagaggataaaatcctagcgacgccaaccctcgccaaggttctaccactgcctgtccgacggattattggtgatttatccgaccgtgagaaagttttgattggccttgatttactctacggcgaacttcaagattccgacgacttctaataatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgacttccgctgagatgactagccctaataataattctgagcaccaagccatcgctaagatgcgcacgatgattgaaggctttgatgatattagtcatggcggtcttccaatcgggcgatcgaccctcgttagtggtacttcaggaaccggcaagacccttttttctattcaatttctctataacggtattatcgagtttgatgagcctggggttttcgttactttcgaagaaaccccgcaagatatcattaaaaacgcccgtagttttggctgggatttagccaagctggtcgatgagggcaaactatttattcttgatgcttcacccgatccagaaggtcaagaggttgttggcggcttcgatctctctgctctgattgagcggattaattatgcaattcaaaagtatcgagcgcggcgggtttcaattgactcggtcacgtccgttttccagcaatatgatgcctcttctgtggttcgccgcgaactctttcggttggtagctcgcctaaaacaaattggggcaactacggtcatgaccaccgagcgtatcgaggaatatggcccgatcgctcgttacggtgttgaggaatttgtctccgataacgtcgtgattctccgcaacgttttggaaggggagcgccgtcgccgcaccctcgaaatcctcaagctacgtggcaccagccacatgaaaggggaatatccgttcacgattacggatcatggcatcaatatcttcccgctcggggcaatgcgccttacgcagcgatcgtcgaacgtgcgtgtttcatctggtgtcgtccgactcgatgaaatgtgtggtgggggcttctttaaggactcaatcattctggcaactggcgctacaggcactggtaaaactctgttagttagccgtttcgttgagaatgcttgtgctaacaaagagcgggcgattctgttcgcttatgaagagtcacgagctcagctgctccgcaatgcctattcatggggaatggactttgaggagatggagcgccaaaacctcctcaaaattgtttgcgcctatcctgaatccgcaggtcttgaagaccatttgcagattattaagtcggagatcaatgactttaagccagctcgtattgcaatcgactccctctctgctttggcgcggggcgttagcaacaatgccttccgccaatttgtaattggtgtcactggctacgcgaaacaagaagaaatcacgggactattcacaaataccagtgatcaatttatgggagcgcattcgattactgactcccatatctcaacaattacggatacgattatcttgctccaatacgtcgagattcgtggcgaaatgtcccgcgccattaacgtcttcaagatgcgcggatcttggcatgacaaagcaatccgcgagttcatgatcagcgacaaagggccggacatcaaggattctttccggaactttgagcggattatttcaggttcgccaacacggattaccgtcgatgagaaaagcgaactctcgcgaattgtgcgcggcgttcaagaaaaagggccggagagctagtag BBa_J36802_sequence 1 atgagccctcgtaaaacctacattctcaagctctacgtcgccggcaatactccaaactcagtccgtgccctcaaaacgctcaagaacattctcgaagttgaatttcaaggtgtttatgctctaaaggtgatcgatgttctcaaaaatcctcagttggcagaagaggataaaatcctagcgacgccaaccctcgccaaggttctaccactgcctgtccgacggattattggtgatttatccgaccgtgagaaagttttgattggccttgatttactctacggcgaacttcaagattccgacgacttctaataa BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K116502_sequence 1 atgggagagtctctgtcaccacaagcactggctcaaccgttgctactgcaactgttcgtcgatacccggcccctgtcacagcacattgtgcagcgggttaaaaatattttggcagcagtagaggcaaccgtccccatcagcttgcaggtgatcaatgtggcggaccagccacaactggtggagtactaccgcctagtcgtcacgcctgccctggttaaaattggtccaggctctcgccaagttctgagtggcatcgacctcaccgatcaattagccaaccagttgccccagtggctggttcagcaagaggccttttttgccgatcgagagccacctgaagtcaacattccgttcacggagctaggccaacccgagacccccgcgttgcagcaggctgatgccttttttcagcttcagcaacaatacgctgatctctcggagcggacaaaattccttgagcaggtcattgctctcgtcgcccacgacctccgcaatccgctaaccgctgctcttttggcggtcgacaccattcaaatccgcagtcaatccttttctgtggcgacagccaaggaaatgcagggactgtgcagtctgttcgatcaggcacgatcgcaattgcgtgaaatcgagcgcatgattgccgagattttggaggcaactcgccactctggcgaaagcttgcggatcaatccccgcgaagtcgtctttgagccgctcttacaacaggttctggaacagttgcatgaacgctggcggagtaagcagcagcagttgatcacggatgttccaggcgacctgcccacgctctacgccgaccctgaccgtctgcggcaggttctggtcaatctgctagacaacgccatcaaatacactccgcccgggggcacaatcacgatcgcagcgctccatcgcacgagccagaaagtgcagatcagcatcagcgacacgggctctggcatccctcgcgaccagctcagcgtcattttcaagaacttggtgcggctttcccgcgatagcagccaagagggctacggcattggactatcggtttgccagcgaattgttcaggcccactttggccgcatctgggttgcttcggaactgggccaaggcagcaccttccacttcacgatgccggtttatcgctacaccatgccctgctaa BBa_J36801_sequence 1 gtgctctcgcaaattgcaatctgcatttgggtggaatcgacggcaattttgcaggattgccagcgggcgctgtcggccgatcgctatcaactccaagtctgtgagtctggcgaaatgctcttggagtatgcccaaacccatcgtgaccaaatcgactgcctgattttagtggcagccaatcccagcttcagggcagttgttcagcagctctgctttgagggagtggtggtaccagcgattgtcgtaggcgatcgcgacagtgaggatcccgatgaaccagccaaagaacagctctatcacagcgctgaactgcacctcggtatccatcagctcgagcaattgccctaccaagttgatgctgcactggctgaatttctgcgcttagccccggtcgagaccatggccgaccacatcatgctgatgggggccaaccacgatcccgagctatcgagccagcagcgggacctcgctcagcgactacaagagcgcctaggctatctcggggtctactacaagcgtgatcccgatcgctttctgcgcaacctacccgcctacgaaagccaaaagctgcaccaagcgatgcagactagctatcgtgaaatcgttttgagctatttttcgccgaatagcaacctcaaccagagcattgacaacttcgtcaacatggctttctttgccgatgttccagtcaccaaagtggtagaaattcacatggagctgatggacgagtttgccaagaagctccgcgtagagggacgttcagaggacattttgctggattatcggctgactttaattgatgtaattgcacatctttgtgagatgtatcgacggtctatcccacgagaaacctgatga BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_B0032_sequence 1 tcacacaggaaag BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K116500_sequence 1 gacggtgttcacaaagttccttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaaacgttagtttgaatggaaagatgcctgc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z