##gff-version 3
##sequence-region BBa_K116530 1 6802
BBa_K116530	.	promoter	1019	1072	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K116530	.	promoter	1061	1066	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K116530	.	non_covalent_binding_site	1044	1062	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K116530	.	promoter	1038	1043	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K116530	.	engineered_region	1019	1072	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K116530	.	non_covalent_binding_site	1019	1037	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K116530	.	terminator	4419	4459	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K116530	.	polyA_site	4446	4459	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116530	.	stem_loop	4426	4445	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116530	.	stop_codon	4452	4452	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116530	.	engineered_region	4419	4459	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116530	.	CDS	154	873	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K116530	.	CDS	154	873	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K116530	.	terminator	882	961	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K116530	.	stem_loop	893	936	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116530	.	engineered_region	882	961	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116530	.	promoter	5014	5213	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K116530	.	promoter	5174	5179	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K116530	.	CDS	5014	5101	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K116530	.	promoter	5150	5155	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K116530	.	non_covalent_binding_site	5102	5139	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K116530	.	start_codon	5186	5186	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K116530	.	non_covalent_binding_site	5179	5213	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K116530	.	engineered_region	5014	5213	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K116530	.	promoter	1	126	.	+	0	ID=BBa_K116500;Name=BBa_K116500
BBa_K116530	.	non_covalent_binding_site	27	88	.	+	0	ID=annotation1994040;Name=OmpR binding;Parent=BBa_K116500
BBa_K116530	.	terminator	3246	3325	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K116530	.	stem_loop	3257	3300	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116530	.	engineered_region	3246	3325	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116530	.	ribosome_entry_site	5222	5233	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K116530	.	conserved_region	5226	5229	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K116530	.	ribosome_entry_site	4676	4687	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K116530	.	conserved_region	4680	4683	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K116530	.	CDS	4694	5005	.	+	0	ID=BBa_J36802;Name=BBa_J36802
BBa_K116530	.	terminator	4331	4410	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K116530	.	stem_loop	4342	4385	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116530	.	engineered_region	4331	4410	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116530	.	terminator	970	1010	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K116530	.	polyA_site	997	1010	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116530	.	stem_loop	977	996	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116530	.	stop_codon	1003	1003	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116530	.	engineered_region	970	1010	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116530	.	ribosome_entry_site	3445	3456	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K116530	.	conserved_region	3449	3452	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K116530	.	terminator	1857	1936	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K116530	.	stem_loop	1868	1911	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116530	.	engineered_region	1857	1936	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116530	.	CDS	3465	4322	.	+	0	ID=BBa_J36801;Name=BBa_J36801
BBa_K116530	.	ribosome_entry_site	2056	2067	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K116530	.	conserved_region	2060	2063	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K116530	.	ribosome_entry_site	135	147	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K116530	.	engineered_region	135	147	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K116530	.	ribosome_entry_site	141	144	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K116530	.	ribosome_entry_site	135	147	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K116530	.	promoter	1994	2047	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K116530	.	promoter	2036	2041	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K116530	.	non_covalent_binding_site	2019	2037	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K116530	.	promoter	2013	2018	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K116530	.	engineered_region	1994	2047	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K116530	.	non_covalent_binding_site	1994	2012	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K116530	.	promoter	4468	4667	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K116530	.	promoter	4628	4633	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K116530	.	CDS	4468	4555	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K116530	.	promoter	4604	4609	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K116530	.	non_covalent_binding_site	4556	4593	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K116530	.	start_codon	4640	4640	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K116530	.	non_covalent_binding_site	4633	4667	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K116530	.	engineered_region	4468	4667	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K116530	.	CDS	1099	1848	.	+	0	ID=BBa_K116501;Name=BBa_K116501
BBa_K116530	.	terminator	3334	3374	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K116530	.	polyA_site	3361	3374	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116530	.	stem_loop	3341	3360	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116530	.	stop_codon	3367	3367	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116530	.	engineered_region	3334	3374	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116530	.	ribosome_entry_site	1081	1092	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K116530	.	conserved_region	1085	1088	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K116530	.	CDS	5240	6802	.	+	0	ID=BBa_J36804;Name=BBa_J36804
BBa_K116530	.	terminator	1945	1985	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K116530	.	polyA_site	1972	1985	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116530	.	stem_loop	1952	1971	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116530	.	stop_codon	1978	1978	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116530	.	engineered_region	1945	1985	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116530	.	promoter	3383	3436	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K116530	.	promoter	3425	3430	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K116530	.	non_covalent_binding_site	3408	3426	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K116530	.	promoter	3402	3407	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K116530	.	engineered_region	3383	3436	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K116530	.	non_covalent_binding_site	3383	3401	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K116530	.	CDS	2074	3237	.	+	0	ID=BBa_K116502;Name=BBa_K116502
>BBa_K116530
gacggtgttcacaaagttccttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgta
gcactttcacggtagcgaaacgttagtttgaatggaaagatgcctgctactagagtcacacaggaaagtactagatgcg
taaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttct
gtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctg
ttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagca
tgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaag
acacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatg
gaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatgg
aatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatact
ccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacg
aaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaata
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatca
gtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaacc
ccgcatcctcgtgatcgatgatgactcagccatcttggagctggtcgccgtcaatctggagatgtctggctatgacgta
cgcaaagctgaggacggcattaaaggtcaggctttagctgttcagctagttcccgacctgatcatgctggatctaatgc
tgccgcgggttgatggctttaccgtctgtcagcgactgcggcgcgatgagcgtactgccgaaattccggtgctgatgct
gaccgccctcggacagactcaggataaggttgaaggcttcaacgcgggtgctgacgattatctgactaagcccttcgaa
gttgaagagatgctggcccgcgtgcgtgccttgctgcggcgcaccgatcgcattccccatgcagcccgccatagcgaaa
ttctcagctacggtccgctgaccctgattcccgagcggtttgaggccatttggttcaaccgcacggtcaagctgactca
cttggaatttgagttgttgcactgcctgttgcaacgccacggccaaacggttgcgccgagcgaaatcctcaaagaagtc
tggggctatgatcccgacgatgacatcgagacgattcgcgtccacatccgtcatctgcgcaccaagctcgagcccgatc
cccggcacccgcgctacatcaaaacggtctatggagcgggctactgccttgagctgccggccgagacggaactccacca
acacgccgatcagtttccttcggcgtcctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactaga
gaaagaggagaaatactagatgggagagtctctgtcaccacaagcactggctcaaccgttgctactgcaactgttcgtc
gatacccggcccctgtcacagcacattgtgcagcgggttaaaaatattttggcagcagtagaggcaaccgtccccatca
gcttgcaggtgatcaatgtggcggaccagccacaactggtggagtactaccgcctagtcgtcacgcctgccctggttaa
aattggtccaggctctcgccaagttctgagtggcatcgacctcaccgatcaattagccaaccagttgccccagtggctg
gttcagcaagaggccttttttgccgatcgagagccacctgaagtcaacattccgttcacggagctaggccaacccgaga
cccccgcgttgcagcaggctgatgccttttttcagcttcagcaacaatacgctgatctctcggagcggacaaaattcct
tgagcaggtcattgctctcgtcgcccacgacctccgcaatccgctaaccgctgctcttttggcggtcgacaccattcaa
atccgcagtcaatccttttctgtggcgacagccaaggaaatgcagggactgtgcagtctgttcgatcaggcacgatcgc
aattgcgtgaaatcgagcgcatgattgccgagattttggaggcaactcgccactctggcgaaagcttgcggatcaatcc
ccgcgaagtcgtctttgagccgctcttacaacaggttctggaacagttgcatgaacgctggcggagtaagcagcagcag
ttgatcacggatgttccaggcgacctgcccacgctctacgccgaccctgaccgtctgcggcaggttctggtcaatctgc
tagacaacgccatcaaatacactccgcccgggggcacaatcacgatcgcagcgctccatcgcacgagccagaaagtgca
gatcagcatcagcgacacgggctctggcatccctcgcgaccagctcagcgtcattttcaagaacttggtgcggctttcc
cgcgatagcagccaagagggctacggcattggactatcggtttgccagcgaattgttcaggcccactttggccgcatct
gggttgcttcggaactgggccaaggcagcaccttccacttcacgatgccggtttatcgctacaccatgccctgctaata
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgata
gagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagaggtgctctcgcaa
attgcaatctgcatttgggtggaatcgacggcaattttgcaggattgccagcgggcgctgtcggccgatcgctatcaac
tccaagtctgtgagtctggcgaaatgctcttggagtatgcccaaacccatcgtgaccaaatcgactgcctgattttagt
ggcagccaatcccagcttcagggcagttgttcagcagctctgctttgagggagtggtggtaccagcgattgtcgtaggc
gatcgcgacagtgaggatcccgatgaaccagccaaagaacagctctatcacagcgctgaactgcacctcggtatccatc
agctcgagcaattgccctaccaagttgatgctgcactggctgaatttctgcgcttagccccggtcgagaccatggccga
ccacatcatgctgatgggggccaaccacgatcccgagctatcgagccagcagcgggacctcgctcagcgactacaagag
cgcctaggctatctcggggtctactacaagcgtgatcccgatcgctttctgcgcaacctacccgcctacgaaagccaaa
agctgcaccaagcgatgcagactagctatcgtgaaatcgttttgagctatttttcgccgaatagcaacctcaaccagag
cattgacaacttcgtcaacatggctttctttgccgatgttccagtcaccaaagtggtagaaattcacatggagctgatg
gacgagtttgccaagaagctccgcgtagagggacgttcagaggacattttgctggattatcggctgactttaattgatg
taattgcacatctttgtgagatgtatcgacggtctatcccacgagaaacctgatgatactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccga
ttcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagct
cactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaattt
cacacatactagagaaagaggagaaatactagatgagccctcgtaaaacctacattctcaagctctacgtcgccggcaa
tactccaaactcagtccgtgccctcaaaacgctcaagaacattctcgaagttgaatttcaaggtgtttatgctctaaag
gtgatcgatgttctcaaaaatcctcagttggcagaagaggataaaatcctagcgacgccaaccctcgccaaggttctac
cactgcctgtccgacggattattggtgatttatccgaccgtgagaaagttttgattggccttgatttactctacggcga
acttcaagattccgacgacttctaataatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcatta
atgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcat
taggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacat
actagagaaagaggagaaatactagatgacttccgctgagatgactagccctaataataattctgagcaccaagccatc
gctaagatgcgcacgatgattgaaggctttgatgatattagtcatggcggtcttccaatcgggcgatcgaccctcgtta
gtggtacttcaggaaccggcaagacccttttttctattcaatttctctataacggtattatcgagtttgatgagcctgg
ggttttcgttactttcgaagaaaccccgcaagatatcattaaaaacgcccgtagttttggctgggatttagccaagctg
gtcgatgagggcaaactatttattcttgatgcttcacccgatccagaaggtcaagaggttgttggcggcttcgatctct
ctgctctgattgagcggattaattatgcaattcaaaagtatcgagcgcggcgggtttcaattgactcggtcacgtccgt
tttccagcaatatgatgcctcttctgtggttcgccgcgaactctttcggttggtagctcgcctaaaacaaattggggca
actacggtcatgaccaccgagcgtatcgaggaatatggcccgatcgctcgttacggtgttgaggaatttgtctccgata
acgtcgtgattctccgcaacgttttggaaggggagcgccgtcgccgcaccctcgaaatcctcaagctacgtggcaccag
ccacatgaaaggggaatatccgttcacgattacggatcatggcatcaatatcttcccgctcggggcaatgcgccttacg
cagcgatcgtcgaacgtgcgtgtttcatctggtgtcgtccgactcgatgaaatgtgtggtgggggcttctttaaggact
caatcattctggcaactggcgctacaggcactggtaaaactctgttagttagccgtttcgttgagaatgcttgtgctaa
caaagagcgggcgattctgttcgcttatgaagagtcacgagctcagctgctccgcaatgcctattcatggggaatggac
tttgaggagatggagcgccaaaacctcctcaaaattgtttgcgcctatcctgaatccgcaggtcttgaagaccatttgc
agattattaagtcggagatcaatgactttaagccagctcgtattgcaatcgactccctctctgctttggcgcggggcgt
tagcaacaatgccttccgccaatttgtaattggtgtcactggctacgcgaaacaagaagaaatcacgggactattcaca
aataccagtgatcaatttatgggagcgcattcgattactgactcccatatctcaacaattacggatacgattatcttgc
tccaatacgtcgagattcgtggcgaaatgtcccgcgccattaacgtcttcaagatgcgcggatcttggcatgacaaagc
aatccgcgagttcatgatcagcgacaaagggccggacatcaaggattctttccggaactttgagcggattatttcaggt
tcgccaacacggattaccgtcgatgagaaaagcgaactctcgcgaattgtgcgcggcgttcaagaaaaagggccggaga
gctagtag