BBa_K116530
type: ComponentDefinition
persistent identity: BBa K116530
display id: BBa_K116530
version: 1
was generated by: iGEM to SBOL conversion
description: cyanobacterial KaiABC oscillator with pOmpF as output
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:33Z
mutable provenance: BBa_K116520 BBa_B0015 BBa_R0040 BBa_K116514
owned by: myers
owned by: james
mutable description: cyanobacterial KaiABC oscillator with pOmpF as output
discontinued: false
dominant: false
group u list: _210_
m user id: 0
owner id: 2083
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N. Takai et al., Proc Natl Acad Sci U S A 103, 12109 (Aug 8, 2006).
star: false
creator: Chun-Ju Yang
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_R0010
component: BBa_B0012
component: BBa_B0010
component: BBa_J36801
component: BBa_B0034
component: BBa_J36802
component: BBa_K116502
component: BBa_R0040
component: BBa_J36804
component: BBa_E0040
component: BBa_B0032
component: BBa_K116501
component: BBa_K116500
sequence annotation: BBa_K116500
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_K116501
sequence annotation: BBa_K116502
sequence annotation: BBa_J36801
sequence annotation: BBa_R0010
sequence annotation: BBa_J36802
sequence annotation: BBa_J36804

BBa_R0010
type: Component
persistent identity: component1995209
display id: component1995209
version: 1
top level: BBa_K116530
definition: LacI
access: public

BBa_B0012
type: Component
persistent identity: component1995205
display id: component1995205
version: 1
top level: BBa_K116530
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1995203
display id: component1995203
version: 1
top level: BBa_K116530
definition: BBa_B0010
access: public

BBa_J36801
type: Component
persistent identity: component1995202
display id: component1995202
version: 1
top level: BBa_K116530
definition: KaiA
access: public

BBa_B0034
type: Component
persistent identity: component1995227
display id: component1995227
version: 1
top level: BBa_K116530
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component1995219
display id: component1995219
version: 1
top level: BBa_K116530
definition: LacI
access: public

BBa_J36802
type: Component
persistent identity: component1995218
display id: component1995218
version: 1
top level: BBa_K116530
definition: KaiB
access: public

BBa_B0034
type: Component
persistent identity: component1995217
display id: component1995217
version: 1
top level: BBa_K116530
definition: BBa_B0034
access: public

BBa_K116502
type: Component
persistent identity: component1995188
display id: component1995188
version: 1
top level: BBa_K116530
definition: SasA
access: public

BBa_B0034
type: Component
persistent identity: component1995187
display id: component1995187
version: 1
top level: BBa_K116530
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component1995181
display id: component1995181
version: 1
top level: BBa_K116530
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component1995177
display id: component1995177
version: 1
top level: BBa_K116530
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component1995201
display id: component1995201
version: 1
top level: BBa_K116530
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component1995195
display id: component1995195
version: 1
top level: BBa_K116530
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component1995191
display id: component1995191
version: 1
top level: BBa_K116530
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1995189
display id: component1995189
version: 1
top level: BBa_K116530
definition: BBa_B0010
access: public

BBa_J36804
type: Component
persistent identity: component1995228
display id: component1995228
version: 1
top level: BBa_K116530
definition: KaiC
access: public

BBa_B0012
type: Component
persistent identity: component1995163
display id: component1995163
version: 1
top level: BBa_K116530
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1995161
display id: component1995161
version: 1
top level: BBa_K116530
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1995160
display id: component1995160
version: 1
top level: BBa_K116530
definition: GFP
access: public

BBa_B0032
type: Component
persistent identity: component1995157
display id: component1995157
version: 1
top level: BBa_K116530
definition: BBa_B0032
access: public

BBa_B0010
type: Component
persistent identity: component1995175
display id: component1995175
version: 1
top level: BBa_K116530
definition: BBa_B0010
access: public

BBa_K116501
type: Component
persistent identity: component1995174
display id: component1995174
version: 1
top level: BBa_K116530
definition: BBa_K116501
access: public

BBa_B0034
type: Component
persistent identity: component1995173
display id: component1995173
version: 1
top level: BBa_K116530
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component1995167
display id: component1995167
version: 1
top level: BBa_K116530
definition: p(tetR)
access: public

BBa_K116500
type: Component
persistent identity: component1995155
display id: component1995155
version: 1
top level: BBa_K116530
definition: BBa_K116500
access: public

BBa_K116500
type: SequenceAnnotation
persistent identity: annotation1995155
display id: annotation1995155
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995155/range1995155/1
component: BBa_K116500

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type: Range
persistent identity: range1995155
display id: range1995155
version: 1
top level: BBa_K116530
direction: direction/reverse
start: 1
end: 126
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1995157
display id: annotation1995157
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995157/range1995157/1
component: BBa_B0032

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type: Range
persistent identity: range1995157
display id: range1995157
version: 1
top level: BBa_K116530
direction: direction/forward
start: 135
end: 147
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1995160
display id: annotation1995160
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995160/range1995160/1
component: BBa_E0040

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type: Range
persistent identity: range1995160
display id: range1995160
version: 1
top level: BBa_K116530
direction: direction/reverse
start: 154
end: 873
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1995161
display id: annotation1995161
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995161/range1995161/1
component: BBa_B0010

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type: Range
persistent identity: range1995161
display id: range1995161
version: 1
top level: BBa_K116530
direction: direction/forward
start: 882
end: 961
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1995163
display id: annotation1995163
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995163/range1995163/1
component: BBa_B0012

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type: Range
persistent identity: range1995163
display id: range1995163
version: 1
top level: BBa_K116530
direction: direction/forward
start: 970
end: 1010
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1995167
display id: annotation1995167
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995167/range1995167/1
component: BBa_R0040

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type: Range
persistent identity: range1995167
display id: range1995167
version: 1
top level: BBa_K116530
direction: direction/forward
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end: 1072
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1995173
display id: annotation1995173
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995173/range1995173/1
component: BBa_B0034

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type: Range
persistent identity: range1995173
display id: range1995173
version: 1
top level: BBa_K116530
direction: direction/reverse
start: 1081
end: 1092
orientation: inline

BBa_K116501
type: SequenceAnnotation
persistent identity: annotation1995174
display id: annotation1995174
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995174/range1995174/1
component: BBa_K116501

https://synbiohub.org/public/igem/BBa_K116530/annotation1995174/range1995174/1
type: Range
persistent identity: range1995174
display id: range1995174
version: 1
top level: BBa_K116530
direction: direction/reverse
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end: 1848
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1995175
display id: annotation1995175
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995175/range1995175/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K116530/annotation1995175/range1995175/1
type: Range
persistent identity: range1995175
display id: range1995175
version: 1
top level: BBa_K116530
direction: direction/forward
start: 1857
end: 1936
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1995177
display id: annotation1995177
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995177/range1995177/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K116530/annotation1995177/range1995177/1
type: Range
persistent identity: range1995177
display id: range1995177
version: 1
top level: BBa_K116530
direction: direction/forward
start: 1945
end: 1985
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1995181
display id: annotation1995181
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995181/range1995181/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K116530/annotation1995181/range1995181/1
type: Range
persistent identity: range1995181
display id: range1995181
version: 1
top level: BBa_K116530
direction: direction/forward
start: 1994
end: 2047
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1995187
display id: annotation1995187
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995187/range1995187/1
component: BBa_B0034

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type: Range
persistent identity: range1995187
display id: range1995187
version: 1
top level: BBa_K116530
direction: direction/reverse
start: 2056
end: 2067
orientation: inline

BBa_K116502
type: SequenceAnnotation
persistent identity: annotation1995188
display id: annotation1995188
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995188/range1995188/1
component: BBa_K116502

https://synbiohub.org/public/igem/BBa_K116530/annotation1995188/range1995188/1
type: Range
persistent identity: range1995188
display id: range1995188
version: 1
top level: BBa_K116530
direction: direction/reverse
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end: 3237
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1995189
display id: annotation1995189
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995189/range1995189/1
component: BBa_B0010

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type: Range
persistent identity: range1995189
display id: range1995189
version: 1
top level: BBa_K116530
direction: direction/forward
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end: 3325
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1995191
display id: annotation1995191
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995191/range1995191/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K116530/annotation1995191/range1995191/1
type: Range
persistent identity: range1995191
display id: range1995191
version: 1
top level: BBa_K116530
direction: direction/forward
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end: 3374
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1995195
display id: annotation1995195
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995195/range1995195/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K116530/annotation1995195/range1995195/1
type: Range
persistent identity: range1995195
display id: range1995195
version: 1
top level: BBa_K116530
direction: direction/forward
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end: 3436
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1995201
display id: annotation1995201
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995201/range1995201/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K116530/annotation1995201/range1995201/1
type: Range
persistent identity: range1995201
display id: range1995201
version: 1
top level: BBa_K116530
direction: direction/reverse
start: 3445
end: 3456
orientation: inline

BBa_J36801
type: SequenceAnnotation
persistent identity: annotation1995202
display id: annotation1995202
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995202/range1995202/1
component: BBa_J36801

https://synbiohub.org/public/igem/BBa_K116530/annotation1995202/range1995202/1
type: Range
persistent identity: range1995202
display id: range1995202
version: 1
top level: BBa_K116530
direction: direction/reverse
start: 3465
end: 4322
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1995203
display id: annotation1995203
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995203/range1995203/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K116530/annotation1995203/range1995203/1
type: Range
persistent identity: range1995203
display id: range1995203
version: 1
top level: BBa_K116530
direction: direction/forward
start: 4331
end: 4410
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1995205
display id: annotation1995205
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995205/range1995205/1
component: BBa_B0012

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type: Range
persistent identity: range1995205
display id: range1995205
version: 1
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direction: direction/forward
start: 4419
end: 4459
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1995209
display id: annotation1995209
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995209/range1995209/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K116530/annotation1995209/range1995209/1
type: Range
persistent identity: range1995209
display id: range1995209
version: 1
top level: BBa_K116530
direction: direction/forward
start: 4468
end: 4667
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1995217
display id: annotation1995217
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995217/range1995217/1
component: BBa_B0034

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type: Range
persistent identity: range1995217
display id: range1995217
version: 1
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direction: direction/reverse
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end: 4687
orientation: inline

BBa_J36802
type: SequenceAnnotation
persistent identity: annotation1995218
display id: annotation1995218
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995218/range1995218/1
component: BBa_J36802

https://synbiohub.org/public/igem/BBa_K116530/annotation1995218/range1995218/1
type: Range
persistent identity: range1995218
display id: range1995218
version: 1
top level: BBa_K116530
direction: direction/reverse
start: 4694
end: 5005
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BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1995219
display id: annotation1995219
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995219/range1995219/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K116530/annotation1995219/range1995219/1
type: Range
persistent identity: range1995219
display id: range1995219
version: 1
top level: BBa_K116530
direction: direction/forward
start: 5014
end: 5213
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1995227
display id: annotation1995227
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995227/range1995227/1
component: BBa_B0034

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type: Range
persistent identity: range1995227
display id: range1995227
version: 1
top level: BBa_K116530
direction: direction/reverse
start: 5222
end: 5233
orientation: inline

BBa_J36804
type: SequenceAnnotation
persistent identity: annotation1995228
display id: annotation1995228
version: 1
top level: BBa_K116530
location: https://synbiohub.org/public/igem/BBa_K116530/annotation1995228/range1995228/1
component: BBa_J36804

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type: Range
persistent identity: range1995228
display id: range1995228
version: 1
top level: BBa_K116530
direction: direction/reverse
start: 5240
end: 6802
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

SasA
type: ComponentDefinition
persistent identity: BBa K116502
display id: BBa_K116502
version: 1
was generated by: iGEM to SBOL conversion
description: SasA (Synechococcus adaptive sensor A)
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:33Z
mutable provenance: Synechococcus elongatus PCC7942
owned by: james
owned by: myers
mutable description: The SasA???RpaA signal transduction system represents an activation output pathway from the cyanobacteria Kai oscillator. SasA is transfer its phosphoryl group to RpaA, which is predicted to activate this RR.
discontinued: false
dominant: false
experience: experience/None
group u list: _210_
m user id: 0
owner id: 2083
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The pst I enzyme site at 718bp - 723bp was mutated (CTGCAG->TTACAA). This mutated construct is convenient for us to clone in standard biobrick part.
star: false
creator: Chun-Ju Yang
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K116502_sequence/1

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_K116500
type: ComponentDefinition
persistent identity: BBa K116500
display id: BBa_K116500
version: 1
was generated by: iGEM to SBOL conversion
description: OmpF promoter that is activated or repressesed by OmpR according to osmolarity.
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:33Z
mutable provenance: Promoter OmpF is activated by phosphorylated OmpR at low osmolarity, and is repressesed by phosphorylated OmpR at high osmolarity.In Escherichia coli, osmoregulation is mediated in part by the actions of such a two-component system consisting of EnvZ and OmpR. These proteins act to control the relative levels of the outer membrane porin genes, ompF and ompC. At low osmo larity, OmpF predominates in the outer membrane, while at high osmolarity the OmpF porin is replaced by OmpC.OmpF has a larger pore and a faster ??ow rate than OmpC.
owned by: james
owned by: myers
mutable description: Promoter OmpF is activated by phosphorylated OmpR at low osmolarity, and is repressesed by phosphorylated OmpR at high osmolarity.In Escherichia coli, osmoregulation is mediated in part by the actions of such a two-component system consisting of EnvZ and OmpR. These proteins act to control the relative levels of the outer membrane porin genes, ompF and ompC. At low osmo larity, OmpF predominates in the outer membrane, while at high osmolarity the OmpF porin is replaced by OmpC.OmpF has a larger pore and a faster ??ow rate than OmpC. Reference: K. Mattison, R. Oropeza, N. Byers, L. J. Kenney, J Mol Biol 315, 497 (Jan 25, 2002).
discontinued: false
dominant: false
group u list: _210_
m user id: 0
owner id: 2083
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The osmolarity range of OmpF sensing still have to be confirmed
star: false
creator: Chun-Ju Yang
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OmpR binding
sequence: https://synbiohub.org/public/igem/BBa_K116500_sequence/1

OmpR binding
type: SequenceAnnotation
persistent identity: annotation1994040
display id: annotation1994040
version: 1
top level: BBa_K116500
location: https://synbiohub.org/public/igem/BBa_K116500/annotation1994040/range1994040/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K116500/annotation1994040/range1994040/1
type: Range
persistent identity: range1994040
display id: range1994040
version: 1
top level: BBa_K116500
direction: direction/forward
start: 27
end: 88
orientation: inline

BBa_K116501
type: ComponentDefinition
persistent identity: BBa K116501
display id: BBa_K116501
version: 1
was generated by: iGEM to SBOL conversion
description: RpaA(regulator of phycobilisome-associated)
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:33Z
mutable provenance: Synechococcus elongatus PCC7942
owned by: james
owned by: myers
mutable description: The SasA???RpaA signal transduction system represents an activation output pathway from the cyanobacteria Kai oscillator. SasA is transfer its phosphoryl group to RpaA, which is predicted to activate this RR.
discontinued: false
dominant: false
group u list: _210_
m user id: 0
owner id: 2083
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The binding affinity to DNA still have to be confirmed
star: false
creator: Chun-Ju Yang
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K116501_sequence/1

KaiA
type: ComponentDefinition
persistent identity: BBa J36801
display id: BBa_J36801
version: 1
was generated by: iGEM to SBOL conversion
description: KaiA coding region
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:47Z
mutable provenance: synthetic; GeneArt AG
owned by: james
owned by: myers
mutable description: KaiA coding region with an extra stop codon at the end and two silent mutations at the EcoRI and PstI cut sites. From Prochlorococcus (Synechococcus) elongatus PCC7942 genome. The actual DNA is synthetic in nature; was synthesized from GeneArt AG. Note that the coding region is bounded by the BioBricks Primers and is on a non-BioBrick vector pGA4 (ampR). The biobricks primers can be cut out along with the KaiA coding sequence with HindIII and SacI. Length of entire plasmid is 3802bp.
discontinued: false
dominant: false
experience: experience/Works
group u list: _65_
m user id: 0
owner id: 1189
owning group id: 65
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -none-
star: false
creator: Zhipeng Sun
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_J36801_sequence/1

KaiB
type: ComponentDefinition
persistent identity: BBa J36802
display id: BBa_J36802
version: 1
was generated by: iGEM to SBOL conversion
description: KaiB coding region
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:47Z
mutable provenance: synthetic; GeneArt AG
owned by: james
owned by: myers
mutable description: KaiB coding region with an extra stop codon at the end. From Prochlorococcus (Synechococcus) elongatus PCC7942 genome. The actual DNA is synthetic in nature; was synthesized from GeneArt AG. Note that the coding region is bounded by the BioBricks Primers and is on a non-BioBrick vector pPCR-Script (ampR). The biobricks primers can be cut out along with the KaiB coding sequence with KpnI and SacI. The length of the entire plasmid is 3220bp.
discontinued: false
dominant: false
experience: experience/None
group u list: _65_
m user id: 0
owner id: 1189
owning group id: 65
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -none-
star: false
creator: Zhipeng Sun, Hetmann Hsieh, Jeffrey Lau, David Ramos
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_J36802_sequence/1

KaiC
type: ComponentDefinition
persistent identity: BBa J36804
display id: BBa_J36804
version: 1
was generated by: iGEM to SBOL conversion
description: KaiC coding region
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:47Z
mutable provenance: synthetic; GeneArt AG
owned by: james
owned by: myers
mutable description: KaiC coding region with an extra stop codon at the end. Two silent point mutations at EcoRI and PstI site. From Prochlorococcus (Synechococcus) elongatus PCC7942 genome. The actual DNA is synthetic in nature; was synthesized from GeneArt AG. Note that the coding region is bounded by the BioBricks Primers and is on a non-BioBrick vector pGA4(ampR). The biobricks primers can be cut out along with the KaiCcoding sequence with KpnI and XhoI. The length of the entire plasmid is 4558bp.
discontinued: false
dominant: false
experience: experience/Works
group u list: _65_
m user id: 0
owner id: 1189
owning group id: 65
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -none-
star: false
creator: Zhipeng Sun, Hetmann Hsieh, Jeffery Lau, Dave Ramos
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_J36804_sequence/1

https://synbiohub.org/public/igem/BBa_K116530_sequence/1
type: Sequence
persistent identity: BBa K116530 sequence
display id: BBa_K116530_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacggtgttcacaaagttccttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaaacgttagtttgaatggaaagatgcctgctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaccccgcatcctcgtgatcgatgatgactcagccatcttggagctggtcgccgtcaatctggagatgtctggctatgacgtacgcaaagctgaggacggcattaaaggtcaggctttagctgttcagctagttcccgacctgatcatgctggatctaatgctgccgcgggttgatggctttaccgtctgtcagcgactgcggcgcgatgagcgtactgccgaaattccggtgctgatgctgaccgccctcggacagactcaggataaggttgaaggcttcaacgcgggtgctgacgattatctgactaagcccttcgaagttgaagagatgctggcccgcgtgcgtgccttgctgcggcgcaccgatcgcattccccatgcagcccgccatagcgaaattctcagctacggtccgctgaccctgattcccgagcggtttgaggccatttggttcaaccgcacggtcaagctgactcacttggaatttgagttgttgcactgcctgttgcaacgccacggccaaacggttgcgccgagcgaaatcctcaaagaagtctggggctatgatcccgacgatgacatcgagacgattcgcgtccacatccgtcatctgcgcaccaagctcgagcccgatccccggcacccgcgctacatcaaaacggtctatggagcgggctactgccttgagctgccggccgagacggaactccaccaacacgccgatcagtttccttcggcgtcctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K116502_sequence/1
type: Sequence
persistent identity: BBa K116502 sequence
display id: BBa_K116502_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggagagtctctgtcaccacaagcactggctcaaccgttgctactgcaactgttcgtcgatacccggcccctgtcacagcacattgtgcagcgggttaaaaatattttggcagcagtagaggcaaccgtccccatcagcttgcaggtgatcaatgtggcggaccagccacaactggtggagtactaccgcctagtcgtcacgcctgccctggttaaaattggtccaggctctcgccaagttctgagtggcatcgacctcaccgatcaattagccaaccagttgccccagtggctggttcagcaagaggccttttttgccgatcgagagccacctgaagtcaacattccgttcacggagctaggccaacccgagacccccgcgttgcagcaggctgatgccttttttcagcttcagcaacaatacgctgatctctcggagcggacaaaattccttgagcaggtcattgctctcgtcgcccacgacctccgcaatccgctaaccgctgctcttttggcggtcgacaccattcaaatccgcagtcaatccttttctgtggcgacagccaaggaaatgcagggactgtgcagtctgttcgatcaggcacgatcgcaattgcgtgaaatcgagcgcatgattgccgagattttggaggcaactcgccactctggcgaaagcttgcggatcaatccccgcgaagtcgtctttgagccgctcttacaacaggttctggaacagttgcatgaacgctggcggagtaagcagcagcagttgatcacggatgttccaggcgacctgcccacgctctacgccgaccctgaccgtctgcggcaggttctggtcaatctgctagacaacgccatcaaatacactccgcccgggggcacaatcacgatcgcagcgctccatcgcacgagccagaaagtgcagatcagcatcagcgacacgggctctggcatccctcgcgaccagctcagcgtcattttcaagaacttggtgcggctttcccgcgatagcagccaagagggctacggcattggactatcggtttgccagcgaattgttcaggcccactttggccgcatctgggttgcttcggaactgggccaaggcagcaccttccacttcacgatgccggtttatcgctacaccatgccctgctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K116500_sequence/1
type: Sequence
persistent identity: BBa K116500 sequence
display id: BBa_K116500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacggtgttcacaaagttccttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaaacgttagtttgaatggaaagatgcctgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K116501_sequence/1
type: Sequence
persistent identity: BBa K116501 sequence
display id: BBa_K116501_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccccgcatcctcgtgatcgatgatgactcagccatcttggagctggtcgccgtcaatctggagatgtctggctatgacgtacgcaaagctgaggacggcattaaaggtcaggctttagctgttcagctagttcccgacctgatcatgctggatctaatgctgccgcgggttgatggctttaccgtctgtcagcgactgcggcgcgatgagcgtactgccgaaattccggtgctgatgctgaccgccctcggacagactcaggataaggttgaaggcttcaacgcgggtgctgacgattatctgactaagcccttcgaagttgaagagatgctggcccgcgtgcgtgccttgctgcggcgcaccgatcgcattccccatgcagcccgccatagcgaaattctcagctacggtccgctgaccctgattcccgagcggtttgaggccatttggttcaaccgcacggtcaagctgactcacttggaatttgagttgttgcactgcctgttgcaacgccacggccaaacggttgcgccgagcgaaatcctcaaagaagtctggggctatgatcccgacgatgacatcgagacgattcgcgtccacatccgtcatctgcgcaccaagctcgagcccgatccccggcacccgcgctacatcaaaacggtctatggagcgggctactgccttgagctgccggccgagacggaactccaccaacacgccgatcagtttccttcggcgtcctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J36801_sequence/1
type: Sequence
persistent identity: BBa J36801 sequence
display id: BBa_J36801_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgctctcgcaaattgcaatctgcatttgggtggaatcgacggcaattttgcaggattgccagcgggcgctgtcggccgatcgctatcaactccaagtctgtgagtctggcgaaatgctcttggagtatgcccaaacccatcgtgaccaaatcgactgcctgattttagtggcagccaatcccagcttcagggcagttgttcagcagctctgctttgagggagtggtggtaccagcgattgtcgtaggcgatcgcgacagtgaggatcccgatgaaccagccaaagaacagctctatcacagcgctgaactgcacctcggtatccatcagctcgagcaattgccctaccaagttgatgctgcactggctgaatttctgcgcttagccccggtcgagaccatggccgaccacatcatgctgatgggggccaaccacgatcccgagctatcgagccagcagcgggacctcgctcagcgactacaagagcgcctaggctatctcggggtctactacaagcgtgatcccgatcgctttctgcgcaacctacccgcctacgaaagccaaaagctgcaccaagcgatgcagactagctatcgtgaaatcgttttgagctatttttcgccgaatagcaacctcaaccagagcattgacaacttcgtcaacatggctttctttgccgatgttccagtcaccaaagtggtagaaattcacatggagctgatggacgagtttgccaagaagctccgcgtagagggacgttcagaggacattttgctggattatcggctgactttaattgatgtaattgcacatctttgtgagatgtatcgacggtctatcccacgagaaacctgatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J36802_sequence/1
type: Sequence
persistent identity: BBa J36802 sequence
display id: BBa_J36802_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagccctcgtaaaacctacattctcaagctctacgtcgccggcaatactccaaactcagtccgtgccctcaaaacgctcaagaacattctcgaagttgaatttcaaggtgtttatgctctaaaggtgatcgatgttctcaaaaatcctcagttggcagaagaggataaaatcctagcgacgccaaccctcgccaaggttctaccactgcctgtccgacggattattggtgatttatccgaccgtgagaaagttttgattggccttgatttactctacggcgaacttcaagattccgacgacttctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J36804_sequence/1
type: Sequence
persistent identity: BBa J36804 sequence
display id: BBa_J36804_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacttccgctgagatgactagccctaataataattctgagcaccaagccatcgctaagatgcgcacgatgattgaaggctttgatgatattagtcatggcggtcttccaatcgggcgatcgaccctcgttagtggtacttcaggaaccggcaagacccttttttctattcaatttctctataacggtattatcgagtttgatgagcctggggttttcgttactttcgaagaaaccccgcaagatatcattaaaaacgcccgtagttttggctgggatttagccaagctggtcgatgagggcaaactatttattcttgatgcttcacccgatccagaaggtcaagaggttgttggcggcttcgatctctctgctctgattgagcggattaattatgcaattcaaaagtatcgagcgcggcgggtttcaattgactcggtcacgtccgttttccagcaatatgatgcctcttctgtggttcgccgcgaactctttcggttggtagctcgcctaaaacaaattggggcaactacggtcatgaccaccgagcgtatcgaggaatatggcccgatcgctcgttacggtgttgaggaatttgtctccgataacgtcgtgattctccgcaacgttttggaaggggagcgccgtcgccgcaccctcgaaatcctcaagctacgtggcaccagccacatgaaaggggaatatccgttcacgattacggatcatggcatcaatatcttcccgctcggggcaatgcgccttacgcagcgatcgtcgaacgtgcgtgtttcatctggtgtcgtccgactcgatgaaatgtgtggtgggggcttctttaaggactcaatcattctggcaactggcgctacaggcactggtaaaactctgttagttagccgtttcgttgagaatgcttgtgctaacaaagagcgggcgattctgttcgcttatgaagagtcacgagctcagctgctccgcaatgcctattcatggggaatggactttgaggagatggagcgccaaaacctcctcaaaattgtttgcgcctatcctgaatccgcaggtcttgaagaccatttgcagattattaagtcggagatcaatgactttaagccagctcgtattgcaatcgactccctctctgctttggcgcggggcgttagcaacaatgccttccgccaatttgtaattggtgtcactggctacgcgaaacaagaagaaatcacgggactattcacaaataccagtgatcaatttatgggagcgcattcgattactgactcccatatctcaacaattacggatacgattatcttgctccaatacgtcgagattcgtggcgaaatgtcccgcgccattaacgtcttcaagatgcgcggatcttggcatgacaaagcaatccgcgagttcatgatcagcgacaaagggccggacatcaaggattctttccggaactttgagcggattatttcaggttcgccaacacggattaccgtcgatgagaaaagcgaactctcgcgaattgtgcgcggcgttcaagaaaaagggccggagagctagtag
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z