LOCUS BBa_K116533 6784 bp DNA linear 26-Nov-2024 DEFINITION cyanobacterial KaiABC oscillator with pOmpC as output ACCESSION BBa_K116533 VERSION BBa_K116533.1 FEATURES Location/Qualifiers misc_feature 13..30 /label=C1 OmpR misc_feature 34..51 /label=C2 OmpR misc_feature 54..71 /label=C3 OmpR promoter 75..80 /label=-35 promoter 98..103 /label=-10 promoter 1..108 /label=BBa_R0082 RBS 117..129 /label=RBS-3\Weak RBS 123..126 /label=RBS misc_feature 117..129 /label=BBa_B0032 RBS 117..129 /label=BBa_B0032 CDS 136..855 /label=GFP protein CDS 136..855 /label=BBa_E0040 stem_loop 875..918 /label=stem_loop misc_feature 864..943 /label=BBa_B0010 terminator 864..943 /label=BBa_B0010 stem_loop 959..978 /label=T7 TE misc_feature 985..985 /label=stop polyA_site 979..992 /label=polya misc_feature 952..992 /label=BBa_B0012 terminator 952..992 /label=BBa_B0012 misc_feature 1001..1019 /label=TetR 1 misc_feature 1001..1054 /label=BBa_R0040 promoter 1020..1025 /label=-35 misc_feature 1026..1044 /label=TetR 2 promoter 1043..1048 /label=-10 promoter 1001..1054 /label=BBa_R0040 misc_feature 1067..1070 /label=conserved RBS 1063..1074 /label=BBa_B0034 CDS 1081..1830 /label=BBa_K116501 stem_loop 1850..1893 /label=stem_loop misc_feature 1839..1918 /label=BBa_B0010 terminator 1839..1918 /label=BBa_B0010 stem_loop 1934..1953 /label=T7 TE misc_feature 1960..1960 /label=stop polyA_site 1954..1967 /label=polya misc_feature 1927..1967 /label=BBa_B0012 terminator 1927..1967 /label=BBa_B0012 misc_feature 1976..1994 /label=TetR 1 misc_feature 1976..2029 /label=BBa_R0040 promoter 1995..2000 /label=-35 misc_feature 2001..2019 /label=TetR 2 promoter 2018..2023 /label=-10 promoter 1976..2029 /label=BBa_R0040 misc_feature 2042..2045 /label=conserved RBS 2038..2049 /label=BBa_B0034 CDS 2056..3219 /label=BBa_K116502 stem_loop 3239..3282 /label=stem_loop misc_feature 3228..3307 /label=BBa_B0010 terminator 3228..3307 /label=BBa_B0010 stem_loop 3323..3342 /label=T7 TE misc_feature 3349..3349 /label=stop polyA_site 3343..3356 /label=polya misc_feature 3316..3356 /label=BBa_B0012 terminator 3316..3356 /label=BBa_B0012 misc_feature 3365..3383 /label=TetR 1 misc_feature 3365..3418 /label=BBa_R0040 promoter 3384..3389 /label=-35 misc_feature 3390..3408 /label=TetR 2 promoter 3407..3412 /label=-10 promoter 3365..3418 /label=BBa_R0040 misc_feature 3431..3434 /label=conserved RBS 3427..3438 /label=BBa_B0034 CDS 3447..4304 /label=BBa_J36801 stem_loop 4324..4367 /label=stem_loop misc_feature 4313..4392 /label=BBa_B0010 terminator 4313..4392 /label=BBa_B0010 stem_loop 4408..4427 /label=T7 TE misc_feature 4434..4434 /label=stop polyA_site 4428..4441 /label=polya misc_feature 4401..4441 /label=BBa_B0012 terminator 4401..4441 /label=BBa_B0012 CDS 4450..4537 /label=end of LacI coding region (inactive) misc_feature 4450..4649 /label=BBa_R0010 misc_feature 4538..4575 /label=CAP binding site promoter 4586..4591 /label=-35 promoter 4610..4615 /label=-10 misc_feature 4615..4649 /label=LacI binding site misc_feature 4622..4622 /label=start promoter 4450..4649 /label=BBa_R0010 misc_feature 4662..4665 /label=conserved RBS 4658..4669 /label=BBa_B0034 CDS 4676..4987 /label=BBa_J36802 CDS 4996..5083 /label=end of LacI coding region (inactive) misc_feature 4996..5195 /label=BBa_R0010 misc_feature 5084..5121 /label=CAP binding site promoter 5132..5137 /label=-35 promoter 5156..5161 /label=-10 misc_feature 5161..5195 /label=LacI binding site misc_feature 5168..5168 /label=start promoter 4996..5195 /label=BBa_R0010 misc_feature 5208..5211 /label=conserved RBS 5204..5215 /label=BBa_B0034 CDS 5222..6784 /label=BBa_J36804 ORIGIN 1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta 61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagtcac 121 acaggaaagt actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt 181 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt 241 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt 301 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca 361 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa 421 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa 481 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac 541 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac 601 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc 661 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta 721 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga 781 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa 841 ctatacaaat aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 901 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 961 ctcaccttcg ggtgggcctt tctgcgttta tatactagag tccctatcag tgatagagat 1021 tgacatccct atcagtgata gagatactga gcactactag agaaagagga gaaatactag 1081 atgaaacccc gcatcctcgt gatcgatgat gactcagcca tcttggagct ggtcgccgtc 1141 aatctggaga tgtctggcta tgacgtacgc aaagctgagg acggcattaa aggtcaggct 1201 ttagctgttc agctagttcc cgacctgatc atgctggatc taatgctgcc gcgggttgat 1261 ggctttaccg tctgtcagcg actgcggcgc gatgagcgta ctgccgaaat tccggtgctg 1321 atgctgaccg ccctcggaca gactcaggat aaggttgaag gcttcaacgc gggtgctgac 1381 gattatctga ctaagccctt cgaagttgaa gagatgctgg cccgcgtgcg tgccttgctg 1441 cggcgcaccg atcgcattcc ccatgcagcc cgccatagcg aaattctcag ctacggtccg 1501 ctgaccctga ttcccgagcg gtttgaggcc atttggttca accgcacggt caagctgact 1561 cacttggaat ttgagttgtt gcactgcctg ttgcaacgcc acggccaaac ggttgcgccg 1621 agcgaaatcc tcaaagaagt ctggggctat gatcccgacg atgacatcga gacgattcgc 1681 gtccacatcc gtcatctgcg caccaagctc gagcccgatc cccggcaccc gcgctacatc 1741 aaaacggtct atggagcggg ctactgcctt gagctgccgg ccgagacgga actccaccaa 1801 cacgccgatc agtttccttc ggcgtcctga tactagagcc aggcatcaaa taaaacgaaa 1861 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 1921 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtccct 1981 atcagtgata gagattgaca tccctatcag tgatagagat actgagcact actagagaaa 2041 gaggagaaat actagatggg agagtctctg tcaccacaag cactggctca accgttgcta 2101 ctgcaactgt tcgtcgatac ccggcccctg tcacagcaca ttgtgcagcg ggttaaaaat 2161 attttggcag cagtagaggc aaccgtcccc atcagcttgc aggtgatcaa tgtggcggac 2221 cagccacaac tggtggagta ctaccgccta gtcgtcacgc ctgccctggt taaaattggt 2281 ccaggctctc gccaagttct gagtggcatc gacctcaccg atcaattagc caaccagttg 2341 ccccagtggc tggttcagca agaggccttt tttgccgatc gagagccacc tgaagtcaac 2401 attccgttca cggagctagg ccaacccgag acccccgcgt tgcagcaggc tgatgccttt 2461 tttcagcttc agcaacaata cgctgatctc tcggagcgga caaaattcct tgagcaggtc 2521 attgctctcg tcgcccacga cctccgcaat ccgctaaccg ctgctctttt ggcggtcgac 2581 accattcaaa tccgcagtca atccttttct gtggcgacag ccaaggaaat gcagggactg 2641 tgcagtctgt tcgatcaggc acgatcgcaa ttgcgtgaaa tcgagcgcat gattgccgag 2701 attttggagg caactcgcca ctctggcgaa agcttgcgga tcaatccccg cgaagtcgtc 2761 tttgagccgc tcttacaaca ggttctggaa cagttgcatg aacgctggcg gagtaagcag 2821 cagcagttga tcacggatgt tccaggcgac ctgcccacgc tctacgccga ccctgaccgt 2881 ctgcggcagg ttctggtcaa tctgctagac aacgccatca aatacactcc gcccgggggc 2941 acaatcacga tcgcagcgct ccatcgcacg agccagaaag tgcagatcag catcagcgac 3001 acgggctctg gcatccctcg cgaccagctc agcgtcattt tcaagaactt ggtgcggctt 3061 tcccgcgata gcagccaaga gggctacggc attggactat cggtttgcca gcgaattgtt 3121 caggcccact ttggccgcat ctgggttgct tcggaactgg gccaaggcag caccttccac 3181 ttcacgatgc cggtttatcg ctacaccatg ccctgctaat actagagcca ggcatcaaat 3241 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 3301 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact 3361 agagtcccta tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta 3421 ctagagaaag aggagaaata ctagaggtgc tctcgcaaat tgcaatctgc atttgggtgg 3481 aatcgacggc aattttgcag gattgccagc gggcgctgtc ggccgatcgc tatcaactcc 3541 aagtctgtga gtctggcgaa atgctcttgg agtatgccca aacccatcgt gaccaaatcg 3601 actgcctgat tttagtggca gccaatccca gcttcagggc agttgttcag cagctctgct 3661 ttgagggagt ggtggtacca gcgattgtcg taggcgatcg cgacagtgag gatcccgatg 3721 aaccagccaa agaacagctc tatcacagcg ctgaactgca cctcggtatc catcagctcg 3781 agcaattgcc ctaccaagtt gatgctgcac tggctgaatt tctgcgctta gccccggtcg 3841 agaccatggc cgaccacatc atgctgatgg gggccaacca cgatcccgag ctatcgagcc 3901 agcagcggga cctcgctcag cgactacaag agcgcctagg ctatctcggg gtctactaca 3961 agcgtgatcc cgatcgcttt ctgcgcaacc tacccgccta cgaaagccaa aagctgcacc 4021 aagcgatgca gactagctat cgtgaaatcg ttttgagcta tttttcgccg aatagcaacc 4081 tcaaccagag cattgacaac ttcgtcaaca tggctttctt tgccgatgtt ccagtcacca 4141 aagtggtaga aattcacatg gagctgatgg acgagtttgc caagaagctc cgcgtagagg 4201 gacgttcaga ggacattttg ctggattatc ggctgacttt aattgatgta attgcacatc 4261 tttgtgagat gtatcgacgg tctatcccac gagaaacctg atgatactag agccaggcat 4321 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 4381 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 4441 atactagagc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 4501 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt 4561 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg 4621 gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat actagatgag 4681 ccctcgtaaa acctacattc tcaagctcta cgtcgccggc aatactccaa actcagtccg 4741 tgccctcaaa acgctcaaga acattctcga agttgaattt caaggtgttt atgctctaaa 4801 ggtgatcgat gttctcaaaa atcctcagtt ggcagaagag gataaaatcc tagcgacgcc 4861 aaccctcgcc aaggttctac cactgcctgt ccgacggatt attggtgatt tatccgaccg 4921 tgagaaagtt ttgattggcc ttgatttact ctacggcgaa cttcaagatt ccgacgactt 4981 ctaataatac tagagcaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 5041 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 5101 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 5161 tgtgtggaat tgtgagcgga taacaatttc acacatacta gagaaagagg agaaatacta 5221 gatgacttcc gctgagatga ctagccctaa taataattct gagcaccaag ccatcgctaa 5281 gatgcgcacg atgattgaag gctttgatga tattagtcat ggcggtcttc caatcgggcg 5341 atcgaccctc gttagtggta cttcaggaac cggcaagacc cttttttcta ttcaatttct 5401 ctataacggt attatcgagt ttgatgagcc tggggttttc gttactttcg aagaaacccc 5461 gcaagatatc attaaaaacg cccgtagttt tggctgggat ttagccaagc tggtcgatga 5521 gggcaaacta tttattcttg atgcttcacc cgatccagaa ggtcaagagg ttgttggcgg 5581 cttcgatctc tctgctctga ttgagcggat taattatgca attcaaaagt atcgagcgcg 5641 gcgggtttca attgactcgg tcacgtccgt tttccagcaa tatgatgcct cttctgtggt 5701 tcgccgcgaa ctctttcggt tggtagctcg cctaaaacaa attggggcaa ctacggtcat 5761 gaccaccgag cgtatcgagg aatatggccc gatcgctcgt tacggtgttg aggaatttgt 5821 ctccgataac gtcgtgattc tccgcaacgt tttggaaggg gagcgccgtc gccgcaccct 5881 cgaaatcctc aagctacgtg gcaccagcca catgaaaggg gaatatccgt tcacgattac 5941 ggatcatggc atcaatatct tcccgctcgg ggcaatgcgc cttacgcagc gatcgtcgaa 6001 cgtgcgtgtt tcatctggtg tcgtccgact cgatgaaatg tgtggtgggg gcttctttaa 6061 ggactcaatc attctggcaa ctggcgctac aggcactggt aaaactctgt tagttagccg 6121 tttcgttgag aatgcttgtg ctaacaaaga gcgggcgatt ctgttcgctt atgaagagtc 6181 acgagctcag ctgctccgca atgcctattc atggggaatg gactttgagg agatggagcg 6241 ccaaaacctc ctcaaaattg tttgcgccta tcctgaatcc gcaggtcttg aagaccattt 6301 gcagattatt aagtcggaga tcaatgactt taagccagct cgtattgcaa tcgactccct 6361 ctctgctttg gcgcggggcg ttagcaacaa tgccttccgc caatttgtaa ttggtgtcac 6421 tggctacgcg aaacaagaag aaatcacggg actattcaca aataccagtg atcaatttat 6481 gggagcgcat tcgattactg actcccatat ctcaacaatt acggatacga ttatcttgct 6541 ccaatacgtc gagattcgtg gcgaaatgtc ccgcgccatt aacgtcttca agatgcgcgg 6601 atcttggcat gacaaagcaa tccgcgagtt catgatcagc gacaaagggc cggacatcaa 6661 ggattctttc cggaactttg agcggattat ttcaggttcg ccaacacgga ttaccgtcga 6721 tgagaaaagc gaactctcgc gaattgtgcg cggcgttcaa gaaaaagggc cggagagcta 6781 gtag //