##gff-version 3
##sequence-region BBa_K116533 1 6784
BBa_K116533	.	CDS	3447	4304	.	+	0	ID=BBa_J36801;Name=BBa_J36801
BBa_K116533	.	terminator	1839	1918	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K116533	.	engineered_region	1839	1918	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116533	.	stem_loop	1850	1893	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116533	.	terminator	3316	3356	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K116533	.	polyA_site	3343	3356	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116533	.	stop_codon	3349	3349	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116533	.	engineered_region	3316	3356	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116533	.	stem_loop	3323	3342	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116533	.	CDS	1081	1830	.	+	0	ID=BBa_K116501;Name=BBa_K116501
BBa_K116533	.	promoter	1001	1054	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K116533	.	engineered_region	1001	1054	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K116533	.	non_covalent_binding_site	1001	1019	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K116533	.	promoter	1043	1048	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K116533	.	non_covalent_binding_site	1026	1044	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K116533	.	promoter	1020	1025	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K116533	.	terminator	864	943	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K116533	.	engineered_region	864	943	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116533	.	stem_loop	875	918	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116533	.	CDS	5222	6784	.	+	0	ID=BBa_J36804;Name=BBa_J36804
BBa_K116533	.	ribosome_entry_site	5204	5215	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K116533	.	conserved_region	5208	5211	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K116533	.	promoter	4450	4649	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K116533	.	non_covalent_binding_site	4538	4575	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K116533	.	promoter	4610	4615	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K116533	.	promoter	4586	4591	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K116533	.	start_codon	4622	4622	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K116533	.	engineered_region	4450	4649	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K116533	.	CDS	4450	4537	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K116533	.	non_covalent_binding_site	4615	4649	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K116533	.	ribosome_entry_site	4658	4669	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K116533	.	conserved_region	4662	4665	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K116533	.	ribosome_entry_site	117	129	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K116533	.	ribosome_entry_site	117	129	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K116533	.	engineered_region	117	129	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K116533	.	ribosome_entry_site	123	126	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K116533	.	ribosome_entry_site	3427	3438	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K116533	.	conserved_region	3431	3434	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K116533	.	terminator	952	992	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K116533	.	polyA_site	979	992	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116533	.	stop_codon	985	985	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116533	.	engineered_region	952	992	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116533	.	stem_loop	959	978	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116533	.	CDS	2056	3219	.	+	0	ID=BBa_K116502;Name=BBa_K116502
BBa_K116533	.	terminator	1927	1967	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K116533	.	polyA_site	1954	1967	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116533	.	stop_codon	1960	1960	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116533	.	engineered_region	1927	1967	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116533	.	stem_loop	1934	1953	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116533	.	ribosome_entry_site	1063	1074	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K116533	.	conserved_region	1067	1070	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K116533	.	terminator	4401	4441	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K116533	.	polyA_site	4428	4441	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116533	.	stop_codon	4434	4434	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116533	.	engineered_region	4401	4441	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116533	.	stem_loop	4408	4427	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116533	.	CDS	136	855	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K116533	.	CDS	136	855	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K116533	.	promoter	3365	3418	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K116533	.	engineered_region	3365	3418	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K116533	.	non_covalent_binding_site	3365	3383	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K116533	.	promoter	3407	3412	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K116533	.	non_covalent_binding_site	3390	3408	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K116533	.	promoter	3384	3389	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K116533	.	terminator	4313	4392	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K116533	.	engineered_region	4313	4392	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116533	.	stem_loop	4324	4367	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116533	.	promoter	4996	5195	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K116533	.	non_covalent_binding_site	5084	5121	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K116533	.	promoter	5156	5161	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K116533	.	promoter	5132	5137	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K116533	.	start_codon	5168	5168	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K116533	.	engineered_region	4996	5195	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K116533	.	CDS	4996	5083	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K116533	.	non_covalent_binding_site	5161	5195	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K116533	.	terminator	3228	3307	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K116533	.	engineered_region	3228	3307	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116533	.	stem_loop	3239	3282	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116533	.	promoter	1976	2029	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K116533	.	engineered_region	1976	2029	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K116533	.	non_covalent_binding_site	1976	1994	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K116533	.	promoter	2018	2023	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K116533	.	non_covalent_binding_site	2001	2019	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K116533	.	promoter	1995	2000	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K116533	.	promoter	1	108	.	+	0	ID=BBa_R0082;Name=BBa_R0082
BBa_K116533	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_K116533	.	non_covalent_binding_site	54	71	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_K116533	.	promoter	98	103	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_K116533	.	promoter	75	80	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_K116533	.	non_covalent_binding_site	13	30	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_K116533	.	ribosome_entry_site	2038	2049	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K116533	.	conserved_region	2042	2045	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K116533	.	CDS	4676	4987	.	+	0	ID=BBa_J36802;Name=BBa_J36802
>BBa_K116533
tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttgga
ttattctgcatttttggggagaatggacttactagagtcacacaggaaagtactagatgcgtaaaggagaagaactttt
cactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaa
ggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttg
tcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgc
catgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaag
tttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacaca
aattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaa
aattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccct
gtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatgg
tccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatc
cctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaccccgcatcctcgtgatcga
tgatgactcagccatcttggagctggtcgccgtcaatctggagatgtctggctatgacgtacgcaaagctgaggacggc
attaaaggtcaggctttagctgttcagctagttcccgacctgatcatgctggatctaatgctgccgcgggttgatggct
ttaccgtctgtcagcgactgcggcgcgatgagcgtactgccgaaattccggtgctgatgctgaccgccctcggacagac
tcaggataaggttgaaggcttcaacgcgggtgctgacgattatctgactaagcccttcgaagttgaagagatgctggcc
cgcgtgcgtgccttgctgcggcgcaccgatcgcattccccatgcagcccgccatagcgaaattctcagctacggtccgc
tgaccctgattcccgagcggtttgaggccatttggttcaaccgcacggtcaagctgactcacttggaatttgagttgtt
gcactgcctgttgcaacgccacggccaaacggttgcgccgagcgaaatcctcaaagaagtctggggctatgatcccgac
gatgacatcgagacgattcgcgtccacatccgtcatctgcgcaccaagctcgagcccgatccccggcacccgcgctaca
tcaaaacggtctatggagcgggctactgccttgagctgccggccgagacggaactccaccaacacgccgatcagtttcc
ttcggcgtcctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgggagagtctctgtcaccacaagcactggctcaaccgttgctactgcaactgttcgtcgatacccggcccctgtca
cagcacattgtgcagcgggttaaaaatattttggcagcagtagaggcaaccgtccccatcagcttgcaggtgatcaatg
tggcggaccagccacaactggtggagtactaccgcctagtcgtcacgcctgccctggttaaaattggtccaggctctcg
ccaagttctgagtggcatcgacctcaccgatcaattagccaaccagttgccccagtggctggttcagcaagaggccttt
tttgccgatcgagagccacctgaagtcaacattccgttcacggagctaggccaacccgagacccccgcgttgcagcagg
ctgatgccttttttcagcttcagcaacaatacgctgatctctcggagcggacaaaattccttgagcaggtcattgctct
cgtcgcccacgacctccgcaatccgctaaccgctgctcttttggcggtcgacaccattcaaatccgcagtcaatccttt
tctgtggcgacagccaaggaaatgcagggactgtgcagtctgttcgatcaggcacgatcgcaattgcgtgaaatcgagc
gcatgattgccgagattttggaggcaactcgccactctggcgaaagcttgcggatcaatccccgcgaagtcgtctttga
gccgctcttacaacaggttctggaacagttgcatgaacgctggcggagtaagcagcagcagttgatcacggatgttcca
ggcgacctgcccacgctctacgccgaccctgaccgtctgcggcaggttctggtcaatctgctagacaacgccatcaaat
acactccgcccgggggcacaatcacgatcgcagcgctccatcgcacgagccagaaagtgcagatcagcatcagcgacac
gggctctggcatccctcgcgaccagctcagcgtcattttcaagaacttggtgcggctttcccgcgatagcagccaagag
ggctacggcattggactatcggtttgccagcgaattgttcaggcccactttggccgcatctgggttgcttcggaactgg
gccaaggcagcaccttccacttcacgatgccggtttatcgctacaccatgccctgctaatactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatc
agtgatagagatactgagcactactagagaaagaggagaaatactagaggtgctctcgcaaattgcaatctgcatttgg
gtggaatcgacggcaattttgcaggattgccagcgggcgctgtcggccgatcgctatcaactccaagtctgtgagtctg
gcgaaatgctcttggagtatgcccaaacccatcgtgaccaaatcgactgcctgattttagtggcagccaatcccagctt
cagggcagttgttcagcagctctgctttgagggagtggtggtaccagcgattgtcgtaggcgatcgcgacagtgaggat
cccgatgaaccagccaaagaacagctctatcacagcgctgaactgcacctcggtatccatcagctcgagcaattgccct
accaagttgatgctgcactggctgaatttctgcgcttagccccggtcgagaccatggccgaccacatcatgctgatggg
ggccaaccacgatcccgagctatcgagccagcagcgggacctcgctcagcgactacaagagcgcctaggctatctcggg
gtctactacaagcgtgatcccgatcgctttctgcgcaacctacccgcctacgaaagccaaaagctgcaccaagcgatgc
agactagctatcgtgaaatcgttttgagctatttttcgccgaatagcaacctcaaccagagcattgacaacttcgtcaa
catggctttctttgccgatgttccagtcaccaaagtggtagaaattcacatggagctgatggacgagtttgccaagaag
ctccgcgtagagggacgttcagaggacattttgctggattatcggctgactttaattgatgtaattgcacatctttgtg
agatgtatcgacggtctatcccacgagaaacctgatgatactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggc
acgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcacccca
ggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaag
aggagaaatactagatgagccctcgtaaaacctacattctcaagctctacgtcgccggcaatactccaaactcagtccg
tgccctcaaaacgctcaagaacattctcgaagttgaatttcaaggtgtttatgctctaaaggtgatcgatgttctcaaa
aatcctcagttggcagaagaggataaaatcctagcgacgccaaccctcgccaaggttctaccactgcctgtccgacgga
ttattggtgatttatccgaccgtgagaaagttttgattggccttgatttactctacggcgaacttcaagattccgacga
cttctaataatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacag
gtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggcttta
cactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaa
atactagatgacttccgctgagatgactagccctaataataattctgagcaccaagccatcgctaagatgcgcacgatg
attgaaggctttgatgatattagtcatggcggtcttccaatcgggcgatcgaccctcgttagtggtacttcaggaaccg
gcaagacccttttttctattcaatttctctataacggtattatcgagtttgatgagcctggggttttcgttactttcga
agaaaccccgcaagatatcattaaaaacgcccgtagttttggctgggatttagccaagctggtcgatgagggcaaacta
tttattcttgatgcttcacccgatccagaaggtcaagaggttgttggcggcttcgatctctctgctctgattgagcgga
ttaattatgcaattcaaaagtatcgagcgcggcgggtttcaattgactcggtcacgtccgttttccagcaatatgatgc
ctcttctgtggttcgccgcgaactctttcggttggtagctcgcctaaaacaaattggggcaactacggtcatgaccacc
gagcgtatcgaggaatatggcccgatcgctcgttacggtgttgaggaatttgtctccgataacgtcgtgattctccgca
acgttttggaaggggagcgccgtcgccgcaccctcgaaatcctcaagctacgtggcaccagccacatgaaaggggaata
tccgttcacgattacggatcatggcatcaatatcttcccgctcggggcaatgcgccttacgcagcgatcgtcgaacgtg
cgtgtttcatctggtgtcgtccgactcgatgaaatgtgtggtgggggcttctttaaggactcaatcattctggcaactg
gcgctacaggcactggtaaaactctgttagttagccgtttcgttgagaatgcttgtgctaacaaagagcgggcgattct
gttcgcttatgaagagtcacgagctcagctgctccgcaatgcctattcatggggaatggactttgaggagatggagcgc
caaaacctcctcaaaattgtttgcgcctatcctgaatccgcaggtcttgaagaccatttgcagattattaagtcggaga
tcaatgactttaagccagctcgtattgcaatcgactccctctctgctttggcgcggggcgttagcaacaatgccttccg
ccaatttgtaattggtgtcactggctacgcgaaacaagaagaaatcacgggactattcacaaataccagtgatcaattt
atgggagcgcattcgattactgactcccatatctcaacaattacggatacgattatcttgctccaatacgtcgagattc
gtggcgaaatgtcccgcgccattaacgtcttcaagatgcgcggatcttggcatgacaaagcaatccgcgagttcatgat
cagcgacaaagggccggacatcaaggattctttccggaactttgagcggattatttcaggttcgccaacacggattacc
gtcgatgagaaaagcgaactctcgcgaattgtgcgcggcgttcaagaaaaagggccggagagctagtag