BBa_K1166002 1 BBa_K1166002 HlyA-tag+Secretion system 2013-09-16T11:00:00Z 2015-05-08T01:09:33Z The HlyB and HlyD sequences come from the parts registry, while the HlyA from the registry had mayor problems, so we took it from the genomic sequence. This secretion device includes all the necessary elements (linker-HlyA secretion signal peptide and the expression cassette for HlyB and HlyD) for the secretion of expression cassettes cloned in the 5??? terminal of the device. This secretion device is usable by Silver standard fusion in its 5??? end for proteins with no stop codon. Codes for both the proteins HlyB and HlyD (native from uropathogenic strains of E.coli), which, along with the constitutively expressed TolC protein, form a secretion channel. This secretion system recognizes proteins tagged with the C-terminal secretion signal peptide HlyA, and secretes them to the exterior of the bacteria. false false _1478_ 0 13053 9 In stock false HlyA C terminal part has a 5' section compatible with Silver Fusion standard. Downstream, HlyB and HlyD are both under the AraC/pBAD promoter to be induced by arabinose. false Luis Mario Leal Garza & Luis Fernando Camarillo Guerrero annotation2351351 1 HlyB range2351351 1 1574 3694 annotation2351373 1 BBa_B0015 range2351373 1 5159 5287 annotation2351346 1 stop range2351346 1 211 216 annotation2351356 1 stop range2351356 1 5153 5158 annotation2351344 1 Linker range2351344 1 1 30 annotation2351353 1 BBa_B0034 range2351353 1 3701 3712 annotation2351355 1 HlyD range2351355 1 3719 5152 annotation2351345 1 HlyA range2351345 1 31 210 annotation2351347 1 BBa_B0015 range2351347 1 217 345 annotation2351349 1 BBa_B0034 range2351349 1 1556 1567 annotation2351348 1 AraC+pBAD range2351348 1 346 1555 annotation2351352 1 stop range2351352 1 3695 3700 annotation2351354 1 spacer range2351354 1 3713 3718 annotation2351350 1 spacer range2351350 1 1568 1573 BBa_K1166002_sequence 1 ggcggtggcggtagcggcggtggcggttctttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcataataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcaaagaggagaaatactagatggactcctgtcacaagatcgactacggcctgtatgcactggaaatcctggctcaataccacaacgtgtcggtcaacccggaagaaattaaacaccgctttgacacggatggtaccggcctgggtctgacgtcgtggctgctggccgcaaagagcctggaactgaaagtgaaacaggtgaagaagacgattgaccgcctgaactttatcagcctgccagctctggtttggcgcgaagatggtcgtcatttcatcctgacgaaagtgtcgaaggaagcaaatcgctatctgatcttcgacctggaacagcgcaatccgcgtgtgctggaacaaagcgaatttgaagcgctgtaccaaggtcacatcattctgattgcctcacgctccagcgttacgggcaaactggccaagttcgattttacctggtttattccggccatcattaaatatcgtaagattttcatcgaaaccctggtcgtgagcgtgtttctgcaactgttcgcactgattacgccgctgtttttccaggtggtcatggataaggtgctggtccatcgtggtttttcgaccctgaacgtgatcacggttgctctgtcagttgtggtggtgtttgaaattattctgagcggtctgcgtacctacatctttgcacactcgacctctcgtatcgacgttgaactgggtgcgaagctgttccgccacctgctggcactgccgattagctactttgaaagccgtcgtgttggtgataccgtggcccgcgtgcgtgaactggaccaaattcgtaattttctgaccggccaggccctgacgtccgtcctggatctgctgttctcgtttattttctttgccgttatgtggtattacagtccgaagctgacgctggtgattctgtttagtctgccgtgctatgcggcatggagcgtttttatcagcccgattctgcgtcgtcgcctggatgacaaatttagccgtaatgccgacaaccagtcatttctggtcgaatccgttaccgcaattaataccatcaaggctatggcagtttcaccgcaaatgaccaacatttgggataaacaactggcaggttacgttgcagcaggtttcaaggtgaccgttctggctaccattggccagcagggtattcaactgatccagaaaacggtgatgatcatcaacctgtggctgggtgctcatctggtgatttctggcgatctgagcattggtcaactgattgccttcaatatgctggcgggccagatcgtggccccggtgatccgtctggcacagatttggcaagacttccaacaggtcggtattagtgtgacgcgtctgggcgacgtgctgaacagcccgaccgaatcctatcatggcaaactggctctgccggaaatcaatggtaacattacgttccgcaatattcgtttccgctacaaaccggattcgccggtgattctggataacatcaacctgtctatcaaacagggtgaagtgattggtattgtgggtcgctccggttcaggtaagagtacgctgaccaaactgatccagcgtttttatatcccggaaaacggtcaagtcctgattgacggccatgatctggcactggcggacccgaactggctgcgccgtcaagtgggtgtggtgctgcaagacaatgtgctgctgaaccgcagcattattgacaatatcagtctggcaaatccgggcatgtcggtggaaaaggtcatctacgctgccaagctggcaggcgcccacgatttcattagcgaactgcgcgaaggctataacacgattgtgggcgaacagggtgcaggcctgtctggcggtcaacgtcagcgcattgcaattgctcgcgcgctggtcaacaatccgaaaattctgatttttgacgaagcgaccagtgccctggattatgaaagcgaacatattatcatgcgtaacatgcacaaaatctgcaagggccgcacggtcattatcattgcgcaccgtctgagtaccgtcaagaacgcggatcgtatcattgtgatggaaaagggtaaaatcgtggaacaaggcaagcataaggaactgctgtcggaaccggaaagcctgtactcgtatctgtatcaactgcaatcggattaataaaaagaggagaaatactagatgaagacgtggctgatgggctttagtgaatttctgctgcgttacaaactggtgtggtcggaaacctggaagattcgcaaacaactggataccccggtgcgcgaaaaagacgaaaacgaatttctgccagcgcatctggaactgattgaaacgccggtcagtcgtcgcccgcgtctggtggcgtattttatcatgggcttcctggttattgccgtcatcctgtccgttctgggtcaggtggaaattgttgcaaccgctaacggcaaactgacgctgagcggtcgctctaaagaaattaagccgatcgaaaatagcatcgtcaaagaaattatcgtgaaggaaggcgaaagcgtgcgtaaaggtgatgtcctgctgaagctgaccgccctgggcgcagaagcagacaccctgaaaacgcagagctctctgctgcaaacccgtctggaacagacgcgctatcaaattctgtcacgctcgatcgaactgaacaaactgccggaactgaagctgccggatgaaccgtactttcagaatgtgagcgaagaagaagtgctgcgtctgaccagcctgattaaagaacagttctctacgtggcagaaccaaaagtaccaaaaggaactgaatctggataaaaagcgtgcagaacgcctgaccattctggctcgtatcaaccgctacgaaaatctgtcacgtgtggaaaaatcgcgcctggatgactttcgtagtctgctgcataaacaggcaattgctaagcacgcagttctggaacaagaaaacaaatatgtcgaagcggccaatgaactgcgcgtgtacaagagccaactggaacaaattgaaagtgaaatcctgtccgccaaagaagaatatcaactggtgacccaactgttcaaaaatgaaattctggataagctgcgtcagaccacggacaacatcgaactgctgaccctggaactggaaaagaacgaagaacgtcagcaagcaagtgtgattcgtgctccggtttccggtaaagtccagcaactgaaggttcataccgaaggcggtgtggttaccacggcagaaacgctgatggtcatcgtgccggaagatgacaccctggaagtgacggctctggttcagaacaaagatattggctttatcaacgttggtcagaatgcgattatcaaggtcgaagccttcccgtacacccgctatggctacctggtcggtaaagtgaagaacattaatctggatgcgatcgaagaccagaaactgggcctggtgttcaatgttattgtctcagtggaagaaaacgatctgtcgaccggcaataaacacattccgctgagttccggtatggcggttaccgccgaaatcaaaacgggtatgcgttctgtcatctcctatctgctgtcgccgctggaagaatctgtcacggaatcgctgcatgaacgttaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z