BBa_K1166005 1 BBa_K1166005 TAT-Apoptin 2013-09-16T11:00:00Z 2015-05-08T01:09:33Z This part was synthesized by Genscript. Codes for the T7 expression cassette for Apoptin composed of a N-terminal TAT translocation peptide, followed by a linker (GGGGS), a histidine tag (6x) and by the same linker fused to Apoptin. Apoptin is a protein from the Chicken Anemia Virus known to cause apoptosis in human cancer cell lines only using a pathway independent from P53 by a phosphorilation in the Threonine 108. false false _1478_ 0 13053 9 In stock false A histidine tag was used for purification and TAT peptide was included for the toxin to penetrate mammalian cells. false Luis Mario Leal Garza & Luis Fernando Camarillo Guerrero annotation2351573 1 spacer range2351573 1 59 64 annotation2351577 1 start range2351577 1 65 67 annotation2351570 1 BBa_I712074 range2351570 1 1 46 annotation2351572 1 BBa_B0034 range2351572 1 47 58 annotation2351580 1 linker range2351580 1 149 178 annotation2351574 1 His tag range2351574 1 68 85 annotation2351578 1 linker range2351578 1 86 115 annotation2351579 1 TAT range2351579 1 116 148 annotation2351581 1 Apoptin range2351581 1 179 676 BBa_K1166005_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgcaaaagaggagaaatactagatgcatcaccatcaccatcacggcggtggcggtagcggcggtggcggttcttatggccgcaaaaagcgtcgccagcgtcgccgtggcggtggcggtagcggcggtggcggttctatgaacgcgctgcaagaagatacgccgccgggtccgtctaccgtctttcgtccgccgacgagctctcgcccgctggaaaccccgcattgccgtgaaattcgcatcggcattgcaggtatcacgattaccctgtccctgtgcggttgtgcaaatgcccgtgcaccgacgctgcgcagcgctaccgcggataacagtgaatccaccggcttcaaaaatgtgccggatctgcgtacggaccaaccgaagccgccgtcaaaaaagcgttcgtgtgacccgagcgaatatcgcgtttctgaactgaaagaaagtctgatcaccacgaccccgagccgtccgcgcaccgcaaagcgtcgcattcgcctgtaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z