BBa_K1166005
1
BBa_K1166005
TAT-Apoptin
2013-09-16T11:00:00Z
2015-05-08T01:09:33Z
This part was synthesized by Genscript.
Codes for the T7 expression cassette for Apoptin composed of a N-terminal TAT translocation peptide, followed by a linker (GGGGS), a histidine tag (6x) and by the same linker fused to Apoptin. Apoptin is a protein from the Chicken Anemia Virus known to cause apoptosis in human cancer cell lines only using a pathway independent from P53 by a phosphorilation in the Threonine 108.
false
false
_1478_
0
13053
9
In stock
false
A histidine tag was used for purification and TAT peptide was included for the toxin to penetrate mammalian cells.
false
Luis Mario Leal Garza & Luis Fernando Camarillo Guerrero
annotation2351573
1
spacer
range2351573
1
59
64
annotation2351577
1
start
range2351577
1
65
67
annotation2351570
1
BBa_I712074
range2351570
1
1
46
annotation2351572
1
BBa_B0034
range2351572
1
47
58
annotation2351580
1
linker
range2351580
1
149
178
annotation2351574
1
His tag
range2351574
1
68
85
annotation2351578
1
linker
range2351578
1
86
115
annotation2351579
1
TAT
range2351579
1
116
148
annotation2351581
1
Apoptin
range2351581
1
179
676
BBa_K1166005_sequence
1
taatacgactcactatagggaatacaagctacttgttctttttgcaaaagaggagaaatactagatgcatcaccatcaccatcacggcggtggcggtagcggcggtggcggttcttatggccgcaaaaagcgtcgccagcgtcgccgtggcggtggcggtagcggcggtggcggttctatgaacgcgctgcaagaagatacgccgccgggtccgtctaccgtctttcgtccgccgacgagctctcgcccgctggaaaccccgcattgccgtgaaattcgcatcggcattgcaggtatcacgattaccctgtccctgtgcggttgtgcaaatgcccgtgcaccgacgctgcgcagcgctaccgcggataacagtgaatccaccggcttcaaaaatgtgccggatctgcgtacggaccaaccgaagccgccgtcaaaaaagcgttcgtgtgacccgagcgaatatcgcgtttctgaactgaaagaaagtctgatcaccacgaccccgagccgtccgcgcaccgcaaagcgtcgcattcgcctgtaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z