LOCUS       BBa_K116631             2147 bp    DNA     linear       05-Nov-2024
DEFINITION  pRE + RBS + HtlB + T
ACCESSION   BBa_K116631
VERSION     BBa_K116631.1  
FEATURES             Location/Qualifiers
     promoter        1..48
                     /label=BBa_K116603
     RBS             57..69
                     /label=RBS-3\Weak
     RBS             63..66
                     /label=RBS
     misc_feature    57..69
                     /label=BBa_B0032
     RBS             57..69
                     /label=BBa_B0032
     CDS             76..2010
                     /label=BBa_K116601
     stem_loop       2030..2073
                     /label=stem_loop
     misc_feature    2019..2098
                     /label=BBa_B0010
     terminator      2019..2098
                     /label=BBa_B0010
     stem_loop       2114..2133
                     /label=T7 TE
     misc_feature    2140..2140
                     /label=stop
     polyA_site      2134..2147
                     /label=polya
     misc_feature    2107..2147
                     /label=BBa_B0012
     terminator      2107..2147
                     /label=BBa_B0012
ORIGIN
        1 agagcctcgt tgcgtttgtt tgcacgaacc atatgtaagt atttccttta ctagagtcac
       61 acaggaaagt actagatggc gaaaaaccta atactctggc tggtcattgc cgttgtgctg
      121 atgtcagtat tccagagctt tgggcccagc gagtctaatg gccgtaaggt ggattactct
      181 accttcctac aagaggtcaa taacgaccag gttcgtgaag cgcgtatcaa cggacgtgaa
      241 atcaacgtta ccaagaaaga tagtaaccgt tataccactt acattccggt tcaggatccg
      301 aaattactgg ataacctgtt gaccaagaac gtcaaggttg tcggtgaacc gcctgaagaa
      361 ccaagcctgc tggcttctat cttcatctcc tggttcccga tgctgttgct gattggtgtc
      421 tggatcttct tcatgcgtca aatgcagggc ggcggtggca aaggtgccat gtcgtttggt
      481 aagagcaaag cgcgcatgct gacggaagat cagatcaaaa cgacctttgc tgacgttgcg
      541 ggctgcgacg aagcaaaaga agaagttgct gaactggttg agtatctgcg cgagccgagc
      601 cgcttccaga aactcggcgg taagatcccg aaaggcgtct tgatggtcgg tcctccgggt
      661 accggtaaaa cgctgctggc gaaagcgatt gcaggcgaag cgaaagttcc gttctttact
      721 atctccggtt ctgacttcgt agaaatgttc gtcggtgtgg gtgcatcccg tgttcgtgac
      781 atgttcgaac aggcgaagaa agcggcaccg tgcatcatct ttatcgatga aatcgacgcc
      841 gtaggccgcc agcgtggcgc tggtctgggc ggtggtcacg atgaacgtga acagactctg
      901 aaccagatgc tggttgagat ggatggcttc gaaggtaacg aaggtatcat cgttatcgcc
      961 gcgactaacc gtccggacgt tctcgacccg gccctgctgc gtcctggccg tttcgaccgt
     1021 caggttgtgg tcggcttgcc agatgttcgc ggtcgtgagc agatcctgaa agttcacatg
     1081 cgtcgcgtac cattggcacc cgatatcgac gcggcaatca ttgcccgtgg tactcctggt
     1141 ttctccggtg ctgacctggc gaacctggtg aacgaagcgg cactgttcgc tgctcgtggc
     1201 aacaaacgcg ttgtgtcgat ggttgagttc gagaaagcga aagacaaaat catgatgggt
     1261 gcggaacgtc gctccatggt gatgacggaa gcgcagaaag aatcgacggc ttaccacgaa
     1321 gcgggtcatg cgattatcgg tcgcctggtg ccggaacacg atccggtgca caaagtgacg
     1381 attatcccac gcggtcgtgc gctgggtgtg actttcttct tgcctgaggg cgacgcaatc
     1441 agcgccagcc gtcagaaact ggaaagccag atttctacgc tgtacggtgg tcgtctggca
     1501 gaagagatca tctacgggcc ggaacatgta tctaccggtg cgtccaacga tattaaagtt
     1561 gcgaccaacc tggcacgtaa catggtgact cagtggggct tctctgagaa attgggtcca
     1621 ctgctgtacg cggaagaaga aggtgaagtg ttcctcggcc gtagcgtagc gaaagcgaaa
     1681 catatgtccg atgaaactgc acgtatcatc gaccaggaag tgaaagcact gattgagcgt
     1741 aactataatc gtgcgcgtca gcttctgacc gacaatatgg atattctgca tgcgatgaaa
     1801 gatgctctca tgaaatatga gactatcgac gcaccgcaga ttgatgacct gatggcacgt
     1861 cgcgatgtac gtccgccagc gggctgggaa gaaccaggcg cttctaacaa ttctggcgac
     1921 aatggtagtc caaaggctcc tcgtccggtt gatgaaccgc gtacgccgaa cccgggtaac
     1981 accatgtcag agcagttagg cgacaagtaa tactagagcc aggcatcaaa taaaacgaaa
     2041 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     2101 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
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