LOCUS       BBa_K116638             1007 bp    DNA     linear       22-Nov-2024
DEFINITION  pLac + RBS + LuxI + T
ACCESSION   BBa_K116638
VERSION     BBa_K116638.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     misc_feature    807..838
                     /label=LVA
     CDS             228..806
                     /label=luxI
     misc_feature    228..845
                     /label=BBa_C0061
     misc_feature    846..870
                     /label=Help:Barcodes
     CDS             228..845
                     /label=BBa_C0061
     stem_loop       890..933
                     /label=stem_loop
     misc_feature    879..958
                     /label=BBa_B0010
     terminator      879..958
                     /label=BBa_B0010
     stem_loop       974..993
                     /label=T7 TE
     misc_feature    1000..1000
                     /label=stop
     polyA_site      994..1007
                     /label=polya
     misc_feature    967..1007
                     /label=BBa_B0012
     terminator      967..1007
                     /label=BBa_B0012
     terminator      879..1007
                     /label=BBa_B0015
     misc_feature    228..1007
                     /label=BBa_K116615
     misc_feature    209..1007
                     /label=BBa_K116625
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg actataatga
      241 taaaaaaatc ggattttttg gcaattccat cggaggagta taaaggtatt ctaagtcttc
      301 gttatcaagt gtttaagcaa agacttgagt gggacttagt tgtagaaaat aaccttgaat
      361 cagatgagta tgataactca aatgcagaat atatttatgc ttgtgatgat actgaaaatg
      421 taagtggatg ctggcgttta ttacctacaa caggtgatta tatgctgaaa agtgtttttc
      481 ctgaattgct tggtcaacag agtgctccca aagatcctaa tatagtcgaa ttaagtcgtt
      541 ttgctgtagg taaaaatagc tcaaagataa ataactctgc tagtgaaatt acaatgaaac
      601 tatttgaagc tatatataaa cacgctgtta gtcaaggtat tacagaatat gtaacagtaa
      661 catcaacagc aatagagcga tttttaaagc gtattaaagt tccttgtcat cgtattggag
      721 acaaagaaat tcatgtatta ggtgatacta aatcggttgt attgtctatg cctattaatg
      781 aacagtttaa aaaagcagtc ttaaatgctg caaacgacga aaactacgct ttagtagctt
      841 aataactctg atagtgctag tgtagatctc tactagagcc aggcatcaaa taaaacgaaa
      901 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
      961 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//