LOCUS       BBa_K116639              454 bp    DNA     linear       26-Dec-2024
DEFINITION  Tuner: pLac + RBS + CIIICd + T
ACCESSION   BBa_K116639
VERSION     BBa_K116639.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     CDS             228..317
                     /label=BBa_K116609
     stem_loop       337..380
                     /label=stem_loop
     misc_feature    326..405
                     /label=BBa_B0010
     terminator      326..405
                     /label=BBa_B0010
     stem_loop       421..440
                     /label=T7 TE
     misc_feature    447..447
                     /label=stop
     polyA_site      441..454
                     /label=polya
     misc_feature    414..454
                     /label=BBa_B0012
     terminator      414..454
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg ccttccgaat
      241 ctttacttga acgaatcacc cgtaaattac gtgacggatg gaaacgcctt atcgacatac
      301 ttaatcagcc aggataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
      361 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
      421 ggctcacctt cgggtgggcc tttctgcgtt tata
//