##gff-version 3
##sequence-region BBa_K116643 1 1598
BBa_K116643	.	ribosome_entry_site	209	221	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K116643	.	ribosome_entry_site	209	221	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K116643	.	engineered_region	209	221	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K116643	.	ribosome_entry_site	215	218	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K116643	.	terminator	618	658	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K116643	.	polyA_site	645	658	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116643	.	stop_codon	651	651	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116643	.	stem_loop	625	644	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116643	.	engineered_region	618	658	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K116643	.	terminator	1470	1549	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K116643	.	stem_loop	1481	1524	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116643	.	engineered_region	1470	1549	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116643	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K116643	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K116643	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K116643	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K116643	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K116643	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K116643	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K116643	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K116643	.	ribosome_entry_site	723	735	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K116643	.	ribosome_entry_site	723	735	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K116643	.	engineered_region	723	735	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K116643	.	ribosome_entry_site	729	732	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K116643	.	terminator	530	609	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K116643	.	stem_loop	541	584	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K116643	.	engineered_region	530	609	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K116643	.	promoter	667	714	.	+	0	ID=BBa_K116603;Name=BBa_K116603
BBa_K116643	.	CDS	742	1461	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K116643	.	CDS	742	1461	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K116643	.	CDS	228	521	.	+	0	ID=BBa_K116602;Name=BBa_K116602
BBa_K116643	.	terminator	1558	1598	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K116643	.	polyA_site	1585	1598	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K116643	.	stop_codon	1591	1591	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K116643	.	stem_loop	1565	1584	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K116643	.	engineered_region	1558	1598	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K116643
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatggttcgtg
caaacaaacgcaacgaggctctacgaatcgagagtgcgttgcttaacaaaatcgcaatgcttggaactgagaagacagc
ggaagctgtgggcgttgataagtcgcagatcagcaggtggaagagggactggattccaaagttctcaatgctgcttgct
gttcttgaatggggggtcgttgacgacgacatggctcgattggcgcgacaagttgctgcgattctcaccaataaaaaac
gcccggcggcaaccgagcgttctgaacaaatccagatggagttctgatactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttatatactagagagagcctcgttgcgtttgtttgcacgaaccatatgtaagtatttc
ctttactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttg
aattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttac
ccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaa
tgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaa
gaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatag
aatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacac
aatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaa
gcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacct
gtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgct
gggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttata