LOCUS       BBa_K116645             1670 bp    DNA     linear       22-Dec-2024
DEFINITION  portable synchronizer
ACCESSION   BBa_K116645
VERSION     BBa_K116645.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     misc_feature    20..21
                     /label=prefix
     misc_feature    193..193
                     /label=T
     misc_feature    511..511
                     /label=A
     CDS             20..769
                     /label=luxR
     misc_feature    20..775
                     /label=BBa_C0062
     misc_feature    776..800
                     /label=Help:Barcodes
     CDS             20..775
                     /label=BBa_C0062
     misc_feature    1..800
                     /label=BBa_K116616
     RBS             809..821
                     /label=RBS-3\Weak
     RBS             815..818
                     /label=RBS
     misc_feature    809..821
                     /label=BBa_B0032
     RBS             809..821
                     /label=BBa_B0032
     misc_feature    1407..1438
                     /label=LVA
     CDS             828..1406
                     /label=luxI
     misc_feature    828..1445
                     /label=BBa_C0061
     misc_feature    1446..1470
                     /label=Help:Barcodes
     CDS             828..1445
                     /label=BBa_C0061
     stem_loop       1490..1533
                     /label=stem_loop
     misc_feature    1479..1558
                     /label=BBa_B0010
     terminator      1479..1558
                     /label=BBa_B0010
     stem_loop       1574..1593
                     /label=T7 TE
     misc_feature    1600..1600
                     /label=stop
     polyA_site      1594..1607
                     /label=polya
     misc_feature    1567..1607
                     /label=BBa_B0012
     terminator      1567..1607
                     /label=BBa_B0012
     terminator      1479..1607
                     /label=BBa_B0015
     misc_feature    828..1607
                     /label=BBa_K116615
     misc_feature    809..1607
                     /label=BBa_K116625
     misc_feature    1..1607
                     /label=BBa_K116634
     misc_feature    1616..1635
                     /label=LuxR/HSL
     promoter        1635..1640
                     /label=-35
     promoter        1657..1662
                     /label=-10
     misc_feature    1668..1668
                     /label=start
     misc_feature    1616..1670
                     /label=BBa_R0062
     promoter        1616..1670
                     /label=BBa_R0062
ORIGIN
        1 tcacacagga aagtactaga tgaaaaacat aaatgccgac gacacataca gaataattaa
       61 taaaattaaa gcttgtagaa gcaataatga tattaatcaa tgcttatctg atatgactaa
      121 aatggtacat tgtgaatatt atttactcgc gatcatttat cctcattcta tggttaaatc
      181 tgatatttca atcctagata attaccctaa aaaatggagg caatattatg atgacgctaa
      241 tttaataaaa tatgatccta tagtagatta ttctaactcc aatcattcac caattaattg
      301 gaatatattt gaaaacaatg ctgtaaataa aaaatctcca aatgtaatta aagaagcgaa
      361 aacatcaggt cttatcactg ggtttagttt ccctattcat acggctaaca atggcttcgg
      421 aatgcttagt tttgcacatt cagaaaaaga caactatata gatagtttat ttttacatgc
      481 gtgtatgaac ataccattaa ttgttccttc tctagttgat aattatcgaa aaataaatat
      541 agcaaataat aaatcaaaca acgatttaac caaaagagaa aaagaatgtt tagcgtgggc
      601 atgcgaagga aaaagctctt gggatatttc aaaaatatta ggttgcagtg agcgtactgt
      661 cactttccat ttaaccaatg cgcaaatgaa actcaataca acaaaccgct gccaaagtat
      721 ttctaaagca attttaacag gagcaattga ttgcccatac tttaaaaatt aataacactg
      781 atagtgctag tgtagatcac tactagagtc acacaggaaa gtactagatg actataatga
      841 taaaaaaatc ggattttttg gcaattccat cggaggagta taaaggtatt ctaagtcttc
      901 gttatcaagt gtttaagcaa agacttgagt gggacttagt tgtagaaaat aaccttgaat
      961 cagatgagta tgataactca aatgcagaat atatttatgc ttgtgatgat actgaaaatg
     1021 taagtggatg ctggcgttta ttacctacaa caggtgatta tatgctgaaa agtgtttttc
     1081 ctgaattgct tggtcaacag agtgctccca aagatcctaa tatagtcgaa ttaagtcgtt
     1141 ttgctgtagg taaaaatagc tcaaagataa ataactctgc tagtgaaatt acaatgaaac
     1201 tatttgaagc tatatataaa cacgctgtta gtcaaggtat tacagaatat gtaacagtaa
     1261 catcaacagc aatagagcga tttttaaagc gtattaaagt tccttgtcat cgtattggag
     1321 acaaagaaat tcatgtatta ggtgatacta aatcggttgt attgtctatg cctattaatg
     1381 aacagtttaa aaaagcagtc ttaaatgctg caaacgacga aaactacgct ttagtagctt
     1441 aataactctg atagtgctag tgtagatctc tactagagcc aggcatcaaa taaaacgaaa
     1501 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1561 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagacctg
     1621 taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataaa
//