LOCUS       BBa_K116646             1520 bp    DNA     linear       21-Nov-2024
DEFINITION  Intermediate part to construct a synchronizer
ACCESSION   BBa_K116646
VERSION     BBa_K116646.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     misc_feature    82..83
                     /label=prefix
     misc_feature    255..255
                     /label=T
     misc_feature    573..573
                     /label=A
     CDS             82..831
                     /label=luxR
     misc_feature    82..837
                     /label=BBa_C0062
     misc_feature    838..862
                     /label=Help:Barcodes
     CDS             82..837
                     /label=BBa_C0062
     misc_feature    63..862
                     /label=BBa_K116616
     stem_loop       882..925
                     /label=stem_loop
     misc_feature    871..950
                     /label=BBa_B0010
     terminator      871..950
                     /label=BBa_B0010
     stem_loop       966..985
                     /label=T7 TE
     misc_feature    992..992
                     /label=stop
     polyA_site      986..999
                     /label=polya
     misc_feature    959..999
                     /label=BBa_B0012
     terminator      959..999
                     /label=BBa_B0012
     terminator      871..999
                     /label=BBa_B0015
     misc_feature    63..999
                     /label=BBa_K116626
     misc_feature    1..999
                     /label=BBa_K116640
     misc_feature    1008..1027
                     /label=LuxR/HSL
     promoter        1027..1032
                     /label=-35
     promoter        1049..1054
                     /label=-10
     misc_feature    1060..1060
                     /label=start
     misc_feature    1008..1062
                     /label=BBa_R0062
     promoter        1008..1062
                     /label=BBa_R0062
     RBS             1071..1083
                     /label=RBS-3\Weak
     RBS             1077..1080
                     /label=RBS
     misc_feature    1071..1083
                     /label=BBa_B0032
     RBS             1071..1083
                     /label=BBa_B0032
     CDS             1090..1383
                     /label=BBa_K116602
     stem_loop       1403..1446
                     /label=stem_loop
     misc_feature    1392..1471
                     /label=BBa_B0010
     terminator      1392..1471
                     /label=BBa_B0010
     stem_loop       1487..1506
                     /label=T7 TE
     misc_feature    1513..1513
                     /label=stop
     polyA_site      1507..1520
                     /label=polya
     misc_feature    1480..1520
                     /label=BBa_B0012
     terminator      1480..1520
                     /label=BBa_B0012
     misc_feature    1008..1520
                     /label=BBa_K116637
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagtacta gatgaaaaac ataaatgccg acgacacata cagaataatt
      121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact
      181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa
      241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct
      301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat
      361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg
      421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc
      481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat
      541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat
      601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg
      661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact
      721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt
      781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac
      841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt
      901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg
     1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag tcacacagga
     1081 aagtactaga tggttcgtgc aaacaaacgc aacgaggctc tacgaatcga gagtgcgttg
     1141 cttaacaaaa tcgcaatgct tggaactgag aagacagcgg aagctgtggg cgttgataag
     1201 tcgcagatca gcaggtggaa gagggactgg attccaaagt tctcaatgct gcttgctgtt
     1261 cttgaatggg gggtcgttga cgacgacatg gctcgattgg cgcgacaagt tgctgcgatt
     1321 ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
     1381 tgatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1441 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     1501 tgggcctttc tgcgtttata
//